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Protein

Ubiquitin domain-containing protein DSK2

Gene

DSK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved, with RAD23 in spindle pole body duplication. Involved in the ubiquitin-proteasome proteolytic pathway.

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • spindle pole body duplication Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-32947-MONOMER.
ReactomeiR-SCE-8856825. Cargo recognition for clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin domain-containing protein DSK2
Gene namesi
Name:DSK2
Synonyms:SHE4
Ordered Locus Names:YMR276W
ORF Names:YM8021.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR276W.
SGDiS000004889. DSK2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149001 – 373Ubiquitin domain-containing protein DSK2Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki13Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki76Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP48510.
PRIDEiP48510.

PTM databases

iPTMnetiP48510.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi35455. 126 interactors.
DIPiDIP-4398N.
IntActiP48510. 32 interactors.
MINTiMINT-548016.
STRINGi4932.YMR276W.

Structurei

Secondary structure

1373
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 9Combined sources9
Beta strandi12 – 18Combined sources7
Helixi24 – 35Combined sources12
Helixi39 – 41Combined sources3
Beta strandi42 – 46Combined sources5
Helixi58 – 60Combined sources3
Beta strandi67 – 72Combined sources6
Helixi328 – 331Combined sources4
Helixi333 – 341Combined sources9
Helixi347 – 357Combined sources11
Helixi361 – 368Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WR1NMR-B328-373[»]
2BWBX-ray2.30A/B/C/D/E/F/G/H/I328-371[»]
2BWEX-ray3.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R328-373[»]
S/T/U1-75[»]
2BWFX-ray1.15A/B1-75[»]
3M63X-ray2.40B1-75[»]
4UN2X-ray1.51B328-373[»]
ProteinModelPortaliP48510.
SMRiP48510.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48510.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76
Domaini327 – 371UBAPROSITE-ProRule annotationAdd BLAST45

Phylogenomic databases

GeneTreeiENSGT00390000005720.
HOGENOMiHOG000216678.
InParanoidiP48510.
KOiK04523.
OMAiIQQMLQA.
OrthoDBiEOG092C5V3D.

Family and domain databases

InterProiView protein in InterPro
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR015496. Ubiquilin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PANTHERiPTHR10677. PTHR10677. 1 hit.
PfamiView protein in Pfam
PF00627. UBA. 1 hit.
PF00240. ubiquitin. 1 hit.
SMARTiView protein in SMART
SM00727. STI1. 2 hits.
SM00165. UBA. 1 hit.
SM00213. UBQ. 1 hit.
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50030. UBA. 1 hit.
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P48510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLNIHIKSG QDKWEVNVAP ESTVLQFKEA INKANGIPVA NQRLIYSGKI
60 70 80 90 100
LKDDQTVESY HIQDGHSVHL VKSQPKPQTA SAAGANNATA TGAAAGTGAT
110 120 130 140 150
PNMSSGQSAG FNPLADLTSA RYAGYLNMPS ADMFGPDGGA LNNDSNNQDE
160 170 180 190 200
LLRMMENPIF QSQMNEMLSN PQMLDFMIQS NPQLQAMGPQ ARQMLQSPMF
210 220 230 240 250
RQMLTNPDMI RQSMQFARMM DPNAGMGSAG GAASAFPAPG GDAPEEGSNT
260 270 280 290 300
NTTSSSNTGN NAGTNAGTNA GANTAANPFA SLLNPALNPF ANAGNAASTG
310 320 330 340 350
MPAFDPALLA SMFQPPVQAS QAEDTRPPEE RYEHQLRQLN DMGFFDFDRN
360 370
VAALRRSGGS VQGALDSLLN GDV
Length:373
Mass (Da):39,346
Last modified:October 5, 2010 - v2
Checksum:i3F8691BB95E46569
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109A → R in AAB07267 (PubMed:8682868).Curated1
Sequence conflicti296A → R in AAB07267 (PubMed:8682868).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40587 Genomic DNA. Translation: AAB07267.1.
Z49704 Genomic DNA. Translation: CAA89774.1.
BK006946 Genomic DNA. Translation: DAA10177.1.
PIRiS54583.
RefSeqiNP_014003.1. NM_001182783.1.

Genome annotation databases

EnsemblFungiiYMR276W; YMR276W; YMR276W.
GeneIDi855319.
KEGGisce:YMR276W.

Similar proteinsi

Entry informationi

Entry nameiDSK2_YEAST
AccessioniPrimary (citable) accession number: P48510
Secondary accession number(s): D6W0A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 5, 2010
Last modified: September 27, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names