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P48510

- DSK2_YEAST

UniProt

P48510 - DSK2_YEAST

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Protein
Ubiquitin domain-containing protein DSK2
Gene
DSK2, SHE4, YMR276W, YM8021.02
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved, with RAD23 in spindle pole body duplication. Involved in the ubiquitin-proteasome proteolytic pathway.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein binding, bridging Source: SGD

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  2. spindle pole body duplication Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32947-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin domain-containing protein DSK2
Gene namesi
Name:DSK2
Synonyms:SHE4
Ordered Locus Names:YMR276W
ORF Names:YM8021.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004889. DSK2.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Ubiquitin domain-containing protein DSK2
PRO_0000114900Add
BLAST

Proteomic databases

MaxQBiP48510.
PaxDbiP48510.
PeptideAtlasiP48510.

Expressioni

Gene expression databases

GenevestigatoriP48510.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ADRM1Q161864EBI-6174,EBI-954387From a different organism.
PSMD4P550362EBI-6174,EBI-359318From a different organism.
PTH2P3422211EBI-6174,EBI-2345448
RPN10P388867EBI-6174,EBI-15949
RPN13O1356310EBI-6174,EBI-32948
RPN13O487264EBI-6174,EBI-7710745From a different organism.
RPT1P332993EBI-6174,EBI-13910
UBCP0CG482EBI-6174,EBI-3390054From a different organism.
UBI4P0CG633EBI-6174,EBI-7000452

Protein-protein interaction databases

BioGridi35455. 256 interactions.
DIPiDIP-4398N.
IntActiP48510. 24 interactions.
MINTiMINT-548016.
STRINGi4932.YMR276W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 99
Beta strandi12 – 187
Helixi24 – 3512
Helixi39 – 413
Beta strandi42 – 465
Helixi58 – 603
Beta strandi67 – 726
Helixi328 – 3314
Helixi333 – 3419
Helixi347 – 35711
Helixi361 – 3699

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WR1NMR-B328-373[»]
2BWBX-ray2.30A/B/C/D/E/F/G/H/I328-371[»]
2BWEX-ray3.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R328-373[»]
S/T/U1-75[»]
2BWFX-ray1.15A/B1-75[»]
3M63X-ray2.40B1-75[»]
ProteinModelPortaliP48510.
SMRiP48510. Positions 1-108, 328-373.

Miscellaneous databases

EvolutionaryTraceiP48510.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7676Ubiquitin-like
Add
BLAST
Domaini327 – 37145UBA
Add
BLAST

Sequence similaritiesi

Contains 1 UBA domain.

Phylogenomic databases

eggNOGiCOG5272.
GeneTreeiENSGT00390000005720.
HOGENOMiHOG000216678.
KOiK04523.
OMAiHLGFGAM.
OrthoDBiEOG71CFWG.

Family and domain databases

InterProiIPR006636. STI1_HS-bd.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR015496. Ubiquilin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PANTHERiPTHR10677. PTHR10677. 1 hit.
PfamiPF00627. UBA. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00727. STI1. 2 hits.
SM00165. UBA. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48510-1 [UniParc]FASTAAdd to Basket

« Hide

MSLNIHIKSG QDKWEVNVAP ESTVLQFKEA INKANGIPVA NQRLIYSGKI    50
LKDDQTVESY HIQDGHSVHL VKSQPKPQTA SAAGANNATA TGAAAGTGAT 100
PNMSSGQSAG FNPLADLTSA RYAGYLNMPS ADMFGPDGGA LNNDSNNQDE 150
LLRMMENPIF QSQMNEMLSN PQMLDFMIQS NPQLQAMGPQ ARQMLQSPMF 200
RQMLTNPDMI RQSMQFARMM DPNAGMGSAG GAASAFPAPG GDAPEEGSNT 250
NTTSSSNTGN NAGTNAGTNA GANTAANPFA SLLNPALNPF ANAGNAASTG 300
MPAFDPALLA SMFQPPVQAS QAEDTRPPEE RYEHQLRQLN DMGFFDFDRN 350
VAALRRSGGS VQGALDSLLN GDV 373
Length:373
Mass (Da):39,346
Last modified:October 5, 2010 - v2
Checksum:i3F8691BB95E46569
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091A → R in AAB07267. 1 Publication
Sequence conflicti296 – 2961A → R in AAB07267. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40587 Genomic DNA. Translation: AAB07267.1.
Z49704 Genomic DNA. Translation: CAA89774.1.
BK006946 Genomic DNA. Translation: DAA10177.1.
PIRiS54583.
RefSeqiNP_014003.1. NM_001182783.1.

Genome annotation databases

EnsemblFungiiYMR276W; YMR276W; YMR276W.
GeneIDi855319.
KEGGisce:YMR276W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40587 Genomic DNA. Translation: AAB07267.1 .
Z49704 Genomic DNA. Translation: CAA89774.1 .
BK006946 Genomic DNA. Translation: DAA10177.1 .
PIRi S54583.
RefSeqi NP_014003.1. NM_001182783.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WR1 NMR - B 328-373 [» ]
2BWB X-ray 2.30 A/B/C/D/E/F/G/H/I 328-371 [» ]
2BWE X-ray 3.10 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R 328-373 [» ]
S/T/U 1-75 [» ]
2BWF X-ray 1.15 A/B 1-75 [» ]
3M63 X-ray 2.40 B 1-75 [» ]
ProteinModelPortali P48510.
SMRi P48510. Positions 1-108, 328-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35455. 256 interactions.
DIPi DIP-4398N.
IntActi P48510. 24 interactions.
MINTi MINT-548016.
STRINGi 4932.YMR276W.

Proteomic databases

MaxQBi P48510.
PaxDbi P48510.
PeptideAtlasi P48510.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR276W ; YMR276W ; YMR276W .
GeneIDi 855319.
KEGGi sce:YMR276W.

Organism-specific databases

SGDi S000004889. DSK2.

Phylogenomic databases

eggNOGi COG5272.
GeneTreei ENSGT00390000005720.
HOGENOMi HOG000216678.
KOi K04523.
OMAi HLGFGAM.
OrthoDBi EOG71CFWG.

Enzyme and pathway databases

BioCyci YEAST:G3O-32947-MONOMER.

Miscellaneous databases

EvolutionaryTracei P48510.
NextBioi 979020.

Gene expression databases

Genevestigatori P48510.

Family and domain databases

InterProi IPR006636. STI1_HS-bd.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR015496. Ubiquilin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view ]
PANTHERi PTHR10677. PTHR10677. 1 hit.
Pfami PF00627. UBA. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view ]
SMARTi SM00727. STI1. 2 hits.
SM00165. UBA. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50030. UBA. 1 hit.
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center."
    Biggins S., Ivanovska I., Rose M.D.
    J. Cell Biol. 133:1331-1346(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition."
    Ohno A., Jee J., Fujiwara K., Tenno T., Goda N., Tochio H., Kobayashi H., Hiroaki H., Shirakawa M.
    Structure 13:521-532(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 328-373.

Entry informationi

Entry nameiDSK2_YEAST
AccessioniPrimary (citable) accession number: P48510
Secondary accession number(s): D6W0A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 5, 2010
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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