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P48510 (DSK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin domain-containing protein DSK2
Gene names
Name:DSK2
Synonyms:SHE4
Ordered Locus Names:YMR276W
ORF Names:YM8021.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved, with RAD23 in spindle pole body duplication. Involved in the ubiquitin-proteasome proteolytic pathway.

Subcellular location

Nucleus Probable.

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 UBA domain.

Contains 1 ubiquitin-like domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ADRM1Q161864EBI-6174,EBI-954387From a different organism.
PSMD4P550362EBI-6174,EBI-359318From a different organism.
PTH2P3422211EBI-6174,EBI-2345448
RPN10P388867EBI-6174,EBI-15949
RPN13O1356310EBI-6174,EBI-32948
RPN13O487264EBI-6174,EBI-7710745From a different organism.
RPT1P332993EBI-6174,EBI-13910
UBCP0CG482EBI-6174,EBI-3390054From a different organism.
UBI4P0CG633EBI-6174,EBI-7000452

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Ubiquitin domain-containing protein DSK2
PRO_0000114900

Regions

Domain1 – 7676Ubiquitin-like
Domain327 – 37145UBA

Experimental info

Sequence conflict1091A → R in AAB07267. Ref.1
Sequence conflict2961A → R in AAB07267. Ref.1

Secondary structure

..................... 373
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P48510 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 3F8691BB95E46569

FASTA37339,346
        10         20         30         40         50         60 
MSLNIHIKSG QDKWEVNVAP ESTVLQFKEA INKANGIPVA NQRLIYSGKI LKDDQTVESY 

        70         80         90        100        110        120 
HIQDGHSVHL VKSQPKPQTA SAAGANNATA TGAAAGTGAT PNMSSGQSAG FNPLADLTSA 

       130        140        150        160        170        180 
RYAGYLNMPS ADMFGPDGGA LNNDSNNQDE LLRMMENPIF QSQMNEMLSN PQMLDFMIQS 

       190        200        210        220        230        240 
NPQLQAMGPQ ARQMLQSPMF RQMLTNPDMI RQSMQFARMM DPNAGMGSAG GAASAFPAPG 

       250        260        270        280        290        300 
GDAPEEGSNT NTTSSSNTGN NAGTNAGTNA GANTAANPFA SLLNPALNPF ANAGNAASTG 

       310        320        330        340        350        360 
MPAFDPALLA SMFQPPVQAS QAEDTRPPEE RYEHQLRQLN DMGFFDFDRN VAALRRSGGS 

       370 
VQGALDSLLN GDV 

« Hide

References

« Hide 'large scale' references
[1]"Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center."
Biggins S., Ivanovska I., Rose M.D.
J. Cell Biol. 133:1331-1346(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition."
Ohno A., Jee J., Fujiwara K., Tenno T., Goda N., Tochio H., Kobayashi H., Hiroaki H., Shirakawa M.
Structure 13:521-532(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 328-373.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L40587 Genomic DNA. Translation: AAB07267.1.
Z49704 Genomic DNA. Translation: CAA89774.1.
BK006946 Genomic DNA. Translation: DAA10177.1.
PIRS54583.
RefSeqNP_014003.1. NM_001182783.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WR1NMR-B328-373[»]
2BWBX-ray2.30A/B/C/D/E/F/G/H/I328-371[»]
2BWEX-ray3.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R328-373[»]
S/T/U1-75[»]
2BWFX-ray1.15A/B1-75[»]
3M63X-ray2.40B1-75[»]
ProteinModelPortalP48510.
SMRP48510. Positions 1-108, 328-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35455. 258 interactions.
DIPDIP-4398N.
IntActP48510. 24 interactions.
MINTMINT-548016.
STRING4932.YMR276W.

Proteomic databases

PaxDbP48510.
PeptideAtlasP48510.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR276W; YMR276W; YMR276W.
GeneID855319.
KEGGsce:YMR276W.

Organism-specific databases

SGDS000004889. DSK2.

Phylogenomic databases

eggNOGCOG5272.
GeneTreeENSGT00390000005720.
HOGENOMHOG000216678.
KOK04523.
OMAPEENRNN.
OrthoDBEOG71CFWG.

Enzyme and pathway databases

BioCycYEAST:G3O-32947-MONOMER.

Gene expression databases

GenevestigatorP48510.

Family and domain databases

InterProIPR006636. STI1_HS-bd.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR015496. Ubiquilin.
IPR000626. Ubiquitin-like.
IPR019954. Ubiquitin_CS.
[Graphical view]
PANTHERPTHR10677. PTHR10677. 1 hit.
PfamPF00627. UBA. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTSM00727. STI1. 2 hits.
SM00165. UBA. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS50030. UBA. 1 hit.
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP48510.
NextBio979020.

Entry information

Entry nameDSK2_YEAST
AccessionPrimary (citable) accession number: P48510
Secondary accession number(s): D6W0A3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 5, 2010
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references