Ubiquitin domain-containing protein DSK2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P48510 (DSK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin domain-containing protein DSK2

Gene names

Name:	DSK2
Synonyms:	SHE4
Ordered Locus Names:	YMR276W
ORF Names:	YM8021.02

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved, with RAD23 in spindle pole body duplication. Involved in the ubiquitin-proteasome proteolytic pathway.
Subcellular location	Nucleus Probable.
Miscellaneous	Present with 3930 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 UBA domain. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from mutant phenotype PubMed 15167887. Source: SGD spindle pole body duplication associated with nuclear envelope Inferred from genetic interaction PubMed 8070654. Source: SGD
Cellular_component	nucleus Inferred by curator PubMed 8070654. Source: SGD
Molecular_function	protein binding, bridging Inferred from mutant phenotype PubMed 11805328. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 373

373

Ubiquitin domain-containing protein DSK2

PRO_0000114900

Regions

Domain

1 – 76

Ubiquitin-like

Domain

327 – 371

UBA

Amino acid modifications

Modified residue

197

Phosphoserine Ref.5

Modified residue

205

Phosphothreonine Ref.5

Modified residue

357

Phosphoserine Ref.5

Experimental info

Sequence conflict

109

A → R in AAB07267. Ref.1

Sequence conflict

296

A → R in AAB07267. Ref.1

Secondary structure

373

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P48510 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 3F8691BB95E46569
Show »

FASTA

373

39,346

        10         20         30         40         50         60 
MSLNIHIKSG QDKWEVNVAP ESTVLQFKEA INKANGIPVA NQRLIYSGKI LKDDQTVESY 

        70         80         90        100        110        120 
HIQDGHSVHL VKSQPKPQTA SAAGANNATA TGAAAGTGAT PNMSSGQSAG FNPLADLTSA 

       130        140        150        160        170        180 
RYAGYLNMPS ADMFGPDGGA LNNDSNNQDE LLRMMENPIF QSQMNEMLSN PQMLDFMIQS 

       190        200        210        220        230        240 
NPQLQAMGPQ ARQMLQSPMF RQMLTNPDMI RQSMQFARMM DPNAGMGSAG GAASAFPAPG 

       250        260        270        280        290        300 
GDAPEEGSNT NTTSSSNTGN NAGTNAGTNA GANTAANPFA SLLNPALNPF ANAGNAASTG 

       310        320        330        340        350        360 
MPAFDPALLA SMFQPPVQAS QAEDTRPPEE RYEHQLRQLN DMGFFDFDRN VAALRRSGGS 

       370 
VQGALDSLLN GDV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center." Biggins S., Ivanovska I., Rose M.D. J. Cell Biol. 133:1331-1346(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197; THR-205 AND SER-357, MASS SPECTROMETRY.
[6]	"Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition." Ohno A., Jee J., Fujiwara K., Tenno T., Goda N., Tochio H., Kobayashi H., Hiroaki H., Shirakawa M. Structure 13:521-532(2005) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 328-373.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L40587 Genomic DNA. Translation: AAB07267.1.
Z49704 Genomic DNA. Translation: CAA89774.1.
BK006946 Genomic DNA. Translation: DAA10177.1.

PIR

S54583.

RefSeq

NP_014003.1. NM_001182783.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WR1	NMR	-	B	328-373	[»]
2BWB	X-ray	2.30	A/B/C/D/E/F/G/H/I	328-371	[»]
2BWE	X-ray	3.10	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R	328-373	[»]
			S/T/U	1-75	[»]
2BWF	X-ray	1.15	A/B	1-75	[»]
3M63	X-ray	2.40	B	1-75	[»]

ProteinModelPortal

P48510.

SMR

P48510. Positions 1-108, 328-373.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4398N.

IntAct

P48510. 11 interactions.

MINT

MINT-548016.

STRING

4932.YMR276W.

Proteomic databases

PaxDb

P48510.

PeptideAtlas

P48510.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YMR276W; YMR276W; YMR276W.

GeneID

855319.

KEGG

sce:YMR276W.

Organism-specific databases

SGD

S000004889. DSK2.

Phylogenomic databases

eggNOG

COG5272.

GeneTree

ENSGT00390000005720.

HOGENOM

HOG000216678.

K04523.

OMA

PSASMFG.

OrthoDB

EOG4T7CCF.

Gene expression databases

Genevestigator

P48510.

GermOnline

YMR276W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR006636. STI1_HS-bd.
IPR009060. UBA-like.
IPR000449. UBA/transl_elong_EF1B_N.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR015496. Ubiquilin.
IPR000626. Ubiquitin.
IPR019954. Ubiquitin_CS.
IPR019955. Ubiquitin_supergroup.
[Graphical view]

PANTHER

PTHR10677. PTHR10677. 1 hit.

Pfam

PF00627. UBA. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]

SMART

SM00727. STI1. 2 hits.
SM00165. UBA. 1 hit.
SM00213. UBQ. 1 hit.
[Graphical view]

SUPFAM

SSF46934. UBA_like. 1 hit.

PROSITE

PS50030. UBA. 1 hit.
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P48510.

NextBio

979020.

Entry information

Entry name

DSK2_YEAST

Accession

Primary (citable) accession number: P48510
Secondary accession number(s): D6W0A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents