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P48510

- DSK2_YEAST

UniProt

P48510 - DSK2_YEAST

Protein

Ubiquitin domain-containing protein DSK2

Gene

DSK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Involved, with RAD23 in spindle pole body duplication. Involved in the ubiquitin-proteasome proteolytic pathway.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein binding, bridging Source: SGD

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    2. spindle pole body duplication Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32947-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin domain-containing protein DSK2
    Gene namesi
    Name:DSK2
    Synonyms:SHE4
    Ordered Locus Names:YMR276W
    ORF Names:YM8021.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004889. DSK2.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Ubiquitin domain-containing protein DSK2PRO_0000114900Add
    BLAST

    Proteomic databases

    MaxQBiP48510.
    PaxDbiP48510.
    PeptideAtlasiP48510.

    Expressioni

    Gene expression databases

    GenevestigatoriP48510.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ADRM1Q161864EBI-6174,EBI-954387From a different organism.
    PSMD4P550362EBI-6174,EBI-359318From a different organism.
    PTH2P3422211EBI-6174,EBI-2345448
    RPN10P388867EBI-6174,EBI-15949
    RPN13O1356310EBI-6174,EBI-32948
    RPN13O487264EBI-6174,EBI-7710745From a different organism.
    RPT1P332993EBI-6174,EBI-13910
    UBCP0CG482EBI-6174,EBI-3390054From a different organism.
    UBI4P0CG633EBI-6174,EBI-7000452

    Protein-protein interaction databases

    BioGridi35455. 256 interactions.
    DIPiDIP-4398N.
    IntActiP48510. 24 interactions.
    MINTiMINT-548016.
    STRINGi4932.YMR276W.

    Structurei

    Secondary structure

    373
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1 – 99
    Beta strandi12 – 187
    Helixi24 – 3512
    Helixi39 – 413
    Beta strandi42 – 465
    Helixi58 – 603
    Beta strandi67 – 726
    Helixi328 – 3314
    Helixi333 – 3419
    Helixi347 – 35711
    Helixi361 – 3699

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WR1NMR-B328-373[»]
    2BWBX-ray2.30A/B/C/D/E/F/G/H/I328-371[»]
    2BWEX-ray3.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R328-373[»]
    S/T/U1-75[»]
    2BWFX-ray1.15A/B1-75[»]
    3M63X-ray2.40B1-75[»]
    ProteinModelPortaliP48510.
    SMRiP48510. Positions 1-108, 328-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP48510.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 7676Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini327 – 37145UBAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 UBA domain.PROSITE-ProRule annotation
    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5272.
    GeneTreeiENSGT00390000005720.
    HOGENOMiHOG000216678.
    KOiK04523.
    OMAiHLGFGAM.
    OrthoDBiEOG71CFWG.

    Family and domain databases

    InterProiIPR006636. STI1_HS-bd.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR015496. Ubiquilin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view]
    PANTHERiPTHR10677. PTHR10677. 1 hit.
    PfamiPF00627. UBA. 1 hit.
    PF00240. ubiquitin. 1 hit.
    [Graphical view]
    SMARTiSM00727. STI1. 2 hits.
    SM00165. UBA. 1 hit.
    SM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50030. UBA. 1 hit.
    PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48510-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLNIHIKSG QDKWEVNVAP ESTVLQFKEA INKANGIPVA NQRLIYSGKI    50
    LKDDQTVESY HIQDGHSVHL VKSQPKPQTA SAAGANNATA TGAAAGTGAT 100
    PNMSSGQSAG FNPLADLTSA RYAGYLNMPS ADMFGPDGGA LNNDSNNQDE 150
    LLRMMENPIF QSQMNEMLSN PQMLDFMIQS NPQLQAMGPQ ARQMLQSPMF 200
    RQMLTNPDMI RQSMQFARMM DPNAGMGSAG GAASAFPAPG GDAPEEGSNT 250
    NTTSSSNTGN NAGTNAGTNA GANTAANPFA SLLNPALNPF ANAGNAASTG 300
    MPAFDPALLA SMFQPPVQAS QAEDTRPPEE RYEHQLRQLN DMGFFDFDRN 350
    VAALRRSGGS VQGALDSLLN GDV 373
    Length:373
    Mass (Da):39,346
    Last modified:October 5, 2010 - v2
    Checksum:i3F8691BB95E46569
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091A → R in AAB07267. (PubMed:8682868)Curated
    Sequence conflicti296 – 2961A → R in AAB07267. (PubMed:8682868)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40587 Genomic DNA. Translation: AAB07267.1.
    Z49704 Genomic DNA. Translation: CAA89774.1.
    BK006946 Genomic DNA. Translation: DAA10177.1.
    PIRiS54583.
    RefSeqiNP_014003.1. NM_001182783.1.

    Genome annotation databases

    EnsemblFungiiYMR276W; YMR276W; YMR276W.
    GeneIDi855319.
    KEGGisce:YMR276W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40587 Genomic DNA. Translation: AAB07267.1 .
    Z49704 Genomic DNA. Translation: CAA89774.1 .
    BK006946 Genomic DNA. Translation: DAA10177.1 .
    PIRi S54583.
    RefSeqi NP_014003.1. NM_001182783.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WR1 NMR - B 328-373 [» ]
    2BWB X-ray 2.30 A/B/C/D/E/F/G/H/I 328-371 [» ]
    2BWE X-ray 3.10 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R 328-373 [» ]
    S/T/U 1-75 [» ]
    2BWF X-ray 1.15 A/B 1-75 [» ]
    3M63 X-ray 2.40 B 1-75 [» ]
    ProteinModelPortali P48510.
    SMRi P48510. Positions 1-108, 328-373.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35455. 256 interactions.
    DIPi DIP-4398N.
    IntActi P48510. 24 interactions.
    MINTi MINT-548016.
    STRINGi 4932.YMR276W.

    Proteomic databases

    MaxQBi P48510.
    PaxDbi P48510.
    PeptideAtlasi P48510.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR276W ; YMR276W ; YMR276W .
    GeneIDi 855319.
    KEGGi sce:YMR276W.

    Organism-specific databases

    SGDi S000004889. DSK2.

    Phylogenomic databases

    eggNOGi COG5272.
    GeneTreei ENSGT00390000005720.
    HOGENOMi HOG000216678.
    KOi K04523.
    OMAi HLGFGAM.
    OrthoDBi EOG71CFWG.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32947-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P48510.
    NextBioi 979020.

    Gene expression databases

    Genevestigatori P48510.

    Family and domain databases

    InterProi IPR006636. STI1_HS-bd.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR015496. Ubiquilin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view ]
    PANTHERi PTHR10677. PTHR10677. 1 hit.
    Pfami PF00627. UBA. 1 hit.
    PF00240. ubiquitin. 1 hit.
    [Graphical view ]
    SMARTi SM00727. STI1. 2 hits.
    SM00165. UBA. 1 hit.
    SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50030. UBA. 1 hit.
    PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center."
      Biggins S., Ivanovska I., Rose M.D.
      J. Cell Biol. 133:1331-1346(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition."
      Ohno A., Jee J., Fujiwara K., Tenno T., Goda N., Tochio H., Kobayashi H., Hiroaki H., Shirakawa M.
      Structure 13:521-532(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 328-373.

    Entry informationi

    Entry nameiDSK2_YEAST
    AccessioniPrimary (citable) accession number: P48510
    Secondary accession number(s): D6W0A3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3930 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3