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Protein

Glutamate--cysteine ligase regulatory subunit

Gene

Gclm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (Gclm), Glutamate--cysteine ligase catalytic subunit (Gclc)
  2. Glutathione synthetase (Gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • enzyme regulator activity Source: GO_Central
  • glutamate-cysteine ligase activity Source: RGD
  • glutamate-cysteine ligase catalytic subunit binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • apoptotic mitochondrial changes Source: Ensembl
  • cellular response to fibroblast growth factor stimulus Source: RGD
  • cellular response to follicle-stimulating hormone stimulus Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to hepatocyte growth factor stimulus Source: RGD
  • cellular response to leukemia inhibitory factor Source: Ensembl
  • cellular response to thyroxine stimulus Source: RGD
  • cysteine metabolic process Source: Ensembl
  • glutamate metabolic process Source: UniProtKB
  • glutathione biosynthetic process Source: RGD
  • hepatic stellate cell activation Source: RGD
  • negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
  • negative regulation of neuron apoptotic process Source: RGD
  • positive regulation of glutamate-cysteine ligase activity Source: RGD
  • regulation of blood vessel size Source: UniProtKB
  • regulation of mitochondrial depolarization Source: Ensembl
  • response to activity Source: RGD
  • response to drug Source: UniProtKB
  • response to human chorionic gonadotropin Source: RGD
  • response to nitrosative stress Source: RGD
  • response to nutrient Source: RGD
  • response to oxidative stress Source: RGD

Keywordsi

Biological processGlutathione biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10025.
BRENDAi6.3.2.2. 5301.
ReactomeiR-RNO-174403. Glutathione synthesis and recycling.
SABIO-RKiP48508.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase regulatory subunit
Alternative name(s):
GCS light chain
Gamma-ECS regulatory subunit
Gamma-glutamylcysteine synthetase regulatory subunit
Glutamate--cysteine ligase modifier subunit
Gene namesi
Name:Gclm
Synonyms:Glclr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi619871. Gclm.

Subcellular locationi

GO - Cellular componenti

  • glutamate-cysteine ligase complex Source: RGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925751 – 274Glutamate--cysteine ligase regulatory subunitAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59PhosphoserineCombined sources1
Modified residuei263N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48508.
PRIDEiP48508.

PTM databases

iPTMnetiP48508.
PhosphoSitePlusiP48508.

Expressioni

Tissue specificityi

Most abundant in kidney. Also found in liver and testis.

Gene expression databases

BgeeiENSRNOG00000013409.
GenevisibleiP48508. RN.

Interactioni

Subunit structurei

Heterodimer of a catalytic heavy chain and a regulatory light chain.

GO - Molecular functioni

  • glutamate-cysteine ligase catalytic subunit binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi248351. 1 interactor.
STRINGi10116.ENSRNOP00000018343.

Structurei

3D structure databases

ProteinModelPortaliP48508.
SMRiP48508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3023. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00510000047658.
HOGENOMiHOG000007111.
HOVERGENiHBG005923.
InParanoidiP48508.
KOiK11205.
OMAiEYLQPYW.
OrthoDBiEOG091G0DYU.
PhylomeDBiP48508.
TreeFamiTF105986.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiView protein in InterPro
IPR032963. Gclm.
IPR023210. NADP_OxRdtase_dom.
PANTHERiPTHR13295. PTHR13295. 1 hit.
PfamiView protein in Pfam
PF00248. Aldo_ket_red. 1 hit.
SUPFAMiSSF51430. SSF51430. 1 hit.

Sequencei

Sequence statusi: Complete.

P48508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTDSRAAGA LLARASTLHL QTGNLLNWGR LRKKCPSTHS EELRDCIQKT
60 70 80 90 100
LNEWSSQISP DLVREFPDVL ECTMSHAVEK INPDEREEMK VSAKLFIVGS
110 120 130 140 150
NSSSSTRNAV DMACSVLGVA QLDSVIMASP PIEDGVNLSL EHLQPYWEEL
160 170 180 190 200
ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL
210 220 230 240 250
TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIEAQ EWVPLWLLRY
260 270
SVIVKSRGII KSKGYILQAK RKGS
Length:274
Mass (Da):30,548
Last modified:February 1, 1996 - v1
Checksum:iC99E35591C96C9CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S65555 mRNA. Translation: AAB28225.1.
L22191 mRNA. Translation: AAA41543.1.
BC078867 mRNA. Translation: AAH78867.1.
PIRiA48019.
RefSeqiNP_059001.1. NM_017305.2.
UniGeneiRn.2460.

Genome annotation databases

EnsembliENSRNOT00000018343; ENSRNOP00000018343; ENSRNOG00000013409.
GeneIDi29739.
KEGGirno:29739.
UCSCiRGD:619871. rat.

Similar proteinsi

Entry informationi

Entry nameiGSH0_RAT
AccessioniPrimary (citable) accession number: P48508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: August 30, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families