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Protein

Triosephosphate isomerase

Gene

Tpi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (LOC500959), Triosephosphate isomerase (LOC246267), Triosephosphate isomerase (Tpi1), Triosephosphate isomerase (LOC100911515), Triosephosphate isomerase (LOC500959)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12SubstrateBy similarity1
Binding sitei14SubstrateBy similarity1
Active sitei96ElectrophileBy similarity1
Active sitei166Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

ReactomeiR-RNO-70171 Glycolysis
R-RNO-70263 Gluconeogenesis
SABIO-RKiP48500
UniPathwayiUPA00109; UER00189
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:Tpi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componentsi: Chromosome 4, Chromosome 9

Organism-specific databases

RGDi3896 Tpi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176801

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000901201 – 249Triosephosphate isomeraseAdd BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14N6-acetyllysineBy similarity1
Modified residuei68Nitrated tyrosineBy similarity1
Modified residuei80PhosphoserineBy similarity1
Modified residuei106PhosphoserineCombined sources1
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei149N6-succinyllysineBy similarity1
Modified residuei156N6-acetyllysine; alternateBy similarity1
Modified residuei156N6-succinyllysine; alternateBy similarity1
Modified residuei173PhosphothreonineBy similarity1
Modified residuei194N6-acetyllysine; alternateBy similarity1
Modified residuei194N6-methyllysine; alternateBy similarity1
Modified residuei194N6-succinyllysine; alternateBy similarity1
Modified residuei198PhosphoserineCombined sources1
Modified residuei209Nitrated tyrosineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei214PhosphothreonineBy similarity1
Modified residuei223PhosphoserineBy similarity1
Modified residuei238N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP48500
PRIDEiP48500

2D gel databases

World-2DPAGEi0004:P48500

PTM databases

iPTMnetiP48500
PhosphoSitePlusiP48500

Expressioni

Gene expression databases

BgeeiENSRNOG00000015290
ExpressionAtlasiP48500 baseline
GenevisibleiP48500 RN

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246967, 3 interactors
CORUMiP48500
IntActiP48500, 2 interactors
STRINGi10116.ENSRNOP00000067409

Structurei

3D structure databases

ProteinModelPortaliP48500
SMRiP48500
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643 Eukaryota
COG0149 LUCA
GeneTreeiENSGT00390000013354
HOGENOMiHOG000226413
HOVERGENiHBG002599
InParanoidiP48500
KOiK01803
OMAiIADCFGT
OrthoDBiEOG091G0LEZ
PhylomeDBiP48500
TreeFamiTF300829

Family and domain databases

CDDicd00311 TIM, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00147_B TIM_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035990 TIM_sf
IPR022896 TrioseP_Isoase_bac/euk
IPR000652 Triosephosphate_isomerase
IPR020861 Triosephosphate_isomerase_AS
PANTHERiPTHR21139 PTHR21139, 1 hit
PfamiView protein in Pfam
PF00121 TIM, 1 hit
SUPFAMiSSF51351 SSF51351, 1 hit
TIGRFAMsiTIGR00419 tim, 1 hit
PROSITEiView protein in PROSITE
PS00171 TIM_1, 1 hit
PS51440 TIM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P48500-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSRKFFVG GNWKMNGRKK CLGELICTLN AAKLPADTEV VCAPPTAYID
60 70 80 90 100
FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDLGAT WVVLGHSERR
110 120 130 140 150
HIFGESDELI GQKVNHALSE GLGVIACIGE KLDEREAGIT EKVVFEQTKA
160 170 180 190 200
IADNVKDWCK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKCNVSEG
210 220 230 240
VAQCTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
Length:249
Mass (Da):26,849
Last modified:January 23, 2007 - v2
Checksum:iBF8AFB389EC8ABD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123G → E in AAA42278 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36250 mRNA Translation: AAA42278.1
BC061781 mRNA Translation: AAH61781.1
BC126087 mRNA Translation: AAI26088.1
RefSeqiNP_075211.2, NM_022922.2
UniGeneiRn.144555
Rn.37838

Genome annotation databases

EnsembliENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290
ENSRNOT00000071439; ENSRNOP00000067409; ENSRNOG00000050669
GeneIDi24849
KEGGirno:24849
UCSCiRGD:3896 rat

Similar proteinsi

Entry informationi

Entry nameiTPIS_RAT
AccessioniPrimary (citable) accession number: P48500
Secondary accession number(s): A0JN18, Q6P793
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health