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Protein

Triosephosphate isomerase

Gene

Tpi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (LOC500959), Triosephosphate isomerase (LOC246267), Triosephosphate isomerase (Tpi1), Triosephosphate isomerase (LOC100911515), Triosephosphate isomerase (LOC500959)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12SubstrateBy similarity1
Binding sitei14SubstrateBy similarity1
Active sitei96ElectrophileBy similarity1
Active sitei166Proton acceptorBy similarity1

GO - Molecular functioni

  • triose-phosphate isomerase activity Source: RGD

GO - Biological processi

  • carbohydrate metabolic process Source: RGD
  • gluconeogenesis Source: GO_Central
  • glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
  • glycerol catabolic process Source: GO_Central
  • glycolytic process Source: RGD
  • pentose-phosphate shunt Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

ReactomeiR-RNO-70171. Glycolysis.
R-RNO-70263. Gluconeogenesis.
SABIO-RKP48500.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:Tpi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componentsi: Chromosome 4, Chromosome 9

Organism-specific databases

RGDi3896. Tpi1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176801.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000901201 – 249Triosephosphate isomeraseAdd BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14N6-acetyllysineBy similarity1
Modified residuei68Nitrated tyrosineBy similarity1
Modified residuei80PhosphoserineBy similarity1
Modified residuei106PhosphoserineCombined sources1
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei149N6-succinyllysineBy similarity1
Modified residuei156N6-acetyllysine; alternateBy similarity1
Modified residuei156N6-succinyllysine; alternateBy similarity1
Modified residuei173PhosphothreonineBy similarity1
Modified residuei194N6-acetyllysine; alternateBy similarity1
Modified residuei194N6-methyllysine; alternateBy similarity1
Modified residuei194N6-succinyllysine; alternateBy similarity1
Modified residuei198PhosphoserineCombined sources1
Modified residuei209Nitrated tyrosineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei214PhosphothreonineBy similarity1
Modified residuei223PhosphoserineBy similarity1
Modified residuei238N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP48500.
PRIDEiP48500.

2D gel databases

World-2DPAGE0004:P48500.

PTM databases

iPTMnetiP48500.
PhosphoSitePlusiP48500.

Expressioni

Gene expression databases

BgeeiENSRNOG00000015290.
ExpressionAtlasiP48500. differential.
GenevisibleiP48500. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi246967. 3 interactors.
IntActiP48500. 2 interactors.
MINTiMINT-4575682.
STRINGi10116.ENSRNOP00000067409.

Structurei

3D structure databases

ProteinModelPortaliP48500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
HOVERGENiHBG002599.
InParanoidiP48500.
KOiK01803.
OMAiMSARKFC.
OrthoDBiEOG091G0LEZ.
PhylomeDBiP48500.
TreeFamiTF300829.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48500-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSRKFFVG GNWKMNGRKK CLGELICTLN AAKLPADTEV VCAPPTAYID
60 70 80 90 100
FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDLGAT WVVLGHSERR
110 120 130 140 150
HIFGESDELI GQKVNHALSE GLGVIACIGE KLDEREAGIT EKVVFEQTKA
160 170 180 190 200
IADNVKDWCK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKCNVSEG
210 220 230 240
VAQCTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
Length:249
Mass (Da):26,849
Last modified:January 23, 2007 - v2
Checksum:iBF8AFB389EC8ABD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123G → E in AAA42278 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36250 mRNA. Translation: AAA42278.1.
BC061781 mRNA. Translation: AAH61781.1.
BC126087 mRNA. Translation: AAI26088.1.
RefSeqiNP_075211.2. NM_022922.2.
UniGeneiRn.144555.
Rn.37838.

Genome annotation databases

EnsembliENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290.
ENSRNOT00000071439; ENSRNOP00000067409; ENSRNOG00000050669.
GeneIDi24849.
KEGGirno:24849.
UCSCiRGD:3896. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36250 mRNA. Translation: AAA42278.1.
BC061781 mRNA. Translation: AAH61781.1.
BC126087 mRNA. Translation: AAI26088.1.
RefSeqiNP_075211.2. NM_022922.2.
UniGeneiRn.144555.
Rn.37838.

3D structure databases

ProteinModelPortaliP48500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246967. 3 interactors.
IntActiP48500. 2 interactors.
MINTiMINT-4575682.
STRINGi10116.ENSRNOP00000067409.

Chemistry databases

ChEMBLiCHEMBL2176801.

PTM databases

iPTMnetiP48500.
PhosphoSitePlusiP48500.

2D gel databases

World-2DPAGE0004:P48500.

Proteomic databases

PaxDbiP48500.
PRIDEiP48500.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290.
ENSRNOT00000071439; ENSRNOP00000067409; ENSRNOG00000050669.
GeneIDi24849.
KEGGirno:24849.
UCSCiRGD:3896. rat.

Organism-specific databases

CTDi7167.
RGDi3896. Tpi1.

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
HOVERGENiHBG002599.
InParanoidiP48500.
KOiK01803.
OMAiMSARKFC.
OrthoDBiEOG091G0LEZ.
PhylomeDBiP48500.
TreeFamiTF300829.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
ReactomeiR-RNO-70171. Glycolysis.
R-RNO-70263. Gluconeogenesis.
SABIO-RKP48500.

Miscellaneous databases

PROiP48500.

Gene expression databases

BgeeiENSRNOG00000015290.
ExpressionAtlasiP48500. differential.
GenevisibleiP48500. RN.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPIS_RAT
AccessioniPrimary (citable) accession number: P48500
Secondary accession number(s): A0JN18, Q6P793
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.