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Protein

Triosephosphate isomerase

Gene

Tpi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (LOC500959), Triosephosphate isomerase (LOC246267), Triosephosphate isomerase (Tpi1), Triosephosphate isomerase (LOC100911515), Triosephosphate isomerase (LOC500959)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121SubstrateBy similarity
Binding sitei14 – 141SubstrateBy similarity
Active sitei96 – 961ElectrophileBy similarity
Active sitei166 – 1661Proton acceptorBy similarity

GO - Molecular functioni

  • triose-phosphate isomerase activity Source: RGD

GO - Biological processi

  • carbohydrate metabolic process Source: RGD
  • gluconeogenesis Source: UniProtKB-UniPathway
  • glycolytic process Source: RGD
  • pentose-phosphate shunt Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

ReactomeiR-RNO-70171. Glycolysis.
R-RNO-70263. Gluconeogenesis.
SABIO-RKP48500.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:Tpi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componentsi: Chromosome 4, Chromosome 9

Organism-specific databases

RGDi3896. Tpi1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2176801.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Triosephosphate isomerasePRO_0000090120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141N6-acetyllysineBy similarity
Modified residuei68 – 681Nitrated tyrosineBy similarity
Modified residuei80 – 801PhosphoserineBy similarity
Modified residuei106 – 1061PhosphoserineCombined sources
Modified residuei149 – 1491N6-succinyllysineBy similarity
Modified residuei156 – 1561N6-acetyllysine; alternateBy similarity
Modified residuei156 – 1561N6-succinyllysine; alternateBy similarity
Modified residuei173 – 1731PhosphothreonineBy similarity
Modified residuei194 – 1941N6-acetyllysine; alternateBy similarity
Modified residuei194 – 1941N6-succinyllysine; alternateBy similarity
Modified residuei198 – 1981PhosphoserineCombined sources
Modified residuei209 – 2091Nitrated tyrosineBy similarity
Modified residuei212 – 2121PhosphoserineBy similarity
Modified residuei214 – 2141PhosphothreonineBy similarity
Modified residuei223 – 2231PhosphoserineBy similarity
Modified residuei238 – 2381N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP48500.
PRIDEiP48500.

2D gel databases

World-2DPAGE0004:P48500.

PTM databases

iPTMnetiP48500.
PhosphoSiteiP48500.

Expressioni

Gene expression databases

ExpressionAtlasiP48500. baseline.
GenevisibleiP48500. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi246967. 3 interactions.
IntActiP48500. 2 interactions.
MINTiMINT-4575682.
STRINGi10116.ENSRNOP00000067409.

Structurei

3D structure databases

ProteinModelPortaliP48500.
SMRiP48500. Positions 3-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
HOVERGENiHBG002599.
InParanoidiP48500.
KOiK01803.
OMAiIAEICKV.
OrthoDBiEOG76DTT8.
PhylomeDBiP48500.
TreeFamiTF300829.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48500-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSRKFFVG GNWKMNGRKK CLGELICTLN AAKLPADTEV VCAPPTAYID
60 70 80 90 100
FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDLGAT WVVLGHSERR
110 120 130 140 150
HIFGESDELI GQKVNHALSE GLGVIACIGE KLDEREAGIT EKVVFEQTKA
160 170 180 190 200
IADNVKDWCK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKCNVSEG
210 220 230 240
VAQCTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
Length:249
Mass (Da):26,849
Last modified:January 23, 2007 - v2
Checksum:iBF8AFB389EC8ABD1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1231G → E in AAA42278 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36250 mRNA. Translation: AAA42278.1.
BC061781 mRNA. Translation: AAH61781.1.
BC126087 mRNA. Translation: AAI26088.1.
RefSeqiNP_075211.2. NM_022922.2.
UniGeneiRn.144555.
Rn.37838.

Genome annotation databases

EnsembliENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290.
ENSRNOT00000071439; ENSRNOP00000067409; ENSRNOG00000050669.
GeneIDi24849.
KEGGirno:24849.
UCSCiRGD:3896. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36250 mRNA. Translation: AAA42278.1.
BC061781 mRNA. Translation: AAH61781.1.
BC126087 mRNA. Translation: AAI26088.1.
RefSeqiNP_075211.2. NM_022922.2.
UniGeneiRn.144555.
Rn.37838.

3D structure databases

ProteinModelPortaliP48500.
SMRiP48500. Positions 3-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246967. 3 interactions.
IntActiP48500. 2 interactions.
MINTiMINT-4575682.
STRINGi10116.ENSRNOP00000067409.

Chemistry

ChEMBLiCHEMBL2176801.

PTM databases

iPTMnetiP48500.
PhosphoSiteiP48500.

2D gel databases

World-2DPAGE0004:P48500.

Proteomic databases

PaxDbiP48500.
PRIDEiP48500.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290.
ENSRNOT00000071439; ENSRNOP00000067409; ENSRNOG00000050669.
GeneIDi24849.
KEGGirno:24849.
UCSCiRGD:3896. rat.

Organism-specific databases

CTDi7167.
RGDi3896. Tpi1.

Phylogenomic databases

eggNOGiKOG1643. Eukaryota.
COG0149. LUCA.
GeneTreeiENSGT00390000013354.
HOGENOMiHOG000226413.
HOVERGENiHBG002599.
InParanoidiP48500.
KOiK01803.
OMAiIAEICKV.
OrthoDBiEOG76DTT8.
PhylomeDBiP48500.
TreeFamiTF300829.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
ReactomeiR-RNO-70171. Glycolysis.
R-RNO-70263. Gluconeogenesis.
SABIO-RKP48500.

Miscellaneous databases

PROiP48500.

Gene expression databases

ExpressionAtlasiP48500. baseline.
GenevisibleiP48500. RN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Yoon K.L., Guidotti G.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Prostate.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 6-14; 34-53; 60-131; 136-188 AND 195-248, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPIS_RAT
AccessioniPrimary (citable) accession number: P48500
Secondary accession number(s): A0JN18, Q6P793
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.