Reviewed, UniProtKB/Swiss-Prot P48496 (TPIC_SPIOL)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase, chloroplastic
      Short name=TIM
      Short name=Triose-phosphate isomerase
    EC=5.3.1.1

Gene names

Name:

TPIP1

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast.
Miscellaneous	In plants, there are two types of TPIS, cytosolic and plastid.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 67	67	Chloroplast
	Chain	68 – 322	255	Triosephosphate isomerase, chloroplastic		PRO_0000035652
Sites
	Active site	162	1	Electrophile By similarity
	Active site	232	1	Proton acceptor By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	80	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48496-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CB47803C402DDDDE	FASTA	322	34,409
	10 20 30 40 50 60 MAVVSTSLAS QITNPNSAVS TQFSGLRRSF LKLENSVSTQ SSFFQNVDSH LRLSSSSRRC 70 80 90 100 110 120 PRGVVAMAGS GKFFVGGNWK CNGTKESITK LVSDLNSATL EADVDVVVAP PFVYIDQVKS 130 140 150 160 170 180 SLTGRVEISA QNCWIGKGGA FTGEISVEQL KDLGCQWVIL GHSERRHVIG EQNEFIGKKA 190 200 210 220 230 240 AYALNQGVGV IACIGELLEE REAGKTFDVC YQQLKAFADA LPSWDNVVVA YEPVWAIGTG 250 260 270 280 290 300 KVASPDQAQE VHVAVRDWLK KNVSEEVASK TRIIYGGSVN GGNCAELAKQ EDIDGFLVGG 310 320 ASLKGPEFAT IVNSVTAKKV AA

References

[1]

"Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme." Henze K., Schnarrenberger C., Kellermann J., Martin W. Plant Mol. Biol. 26:1961-1973(1994) [PubMed: 7858230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Monnopa. Tissue: Leaf and Seedling.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L36387 mRNA. Translation: AAA66289.1.
PIR	S52032.
3D structure databases
HSSP	HSSP built from PDB template 1TPH based on UniProtKB P00940.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.3.1.1. 286.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIC_SPIOL

Accession

Primary (citable) accession number: P48496

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)