Reviewed, UniProtKB/Swiss-Prot P48494 (TPIS_ORYSJ)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase, cytosolic
      Short name=TIM
      Short name=Triose-phosphate isomerase
    EC=5.3.1.1

Gene names

Name:	TPI
Ordered Locus Names:	Os01g0147900, LOC_Os01g05490
ORF Names:	P0416D03.45, P0434B04.9

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Miscellaneous	In plants, there are two types of TPIS, cytosolic and plastid.
Sequence similarities	Belongs to the triosephosphate isomerase family.
Sequence caution	The sequence AAB63603.1 differs from that shown. Reason: Frameshift at position 242.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Gluconeogenesis Glycolysis Lipid synthesis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 253	252	Triosephosphate isomerase, cytosolic		PRO_0000090151
Sites
	Active site	96	1	Electrophile By similarity
	Active site	166	1	Proton acceptor By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	12	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48494-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EF3DE09B89A2A787	FASTA	253	27,063
	10 20 30 40 50 60 MGRKFFVGGN WKCNGTTDQV DKIVKILNEG QIASTDVVEV VVSPPYVFLP VVKSQLRPEI 70 80 90 100 110 120 QVAAQNCWVK KGGAFTGEVS AEMLVNLSIP WVILGHSERR SLLGESNEFV GDKVAYALSQ 130 140 150 160 170 180 GLKVIACVGE TLEQRESGST MDVVAAQTKA ISERIKDWTN VVVAYEPVWA IGTGKVATPD 190 200 210 220 230 240 QAQEVHDGLR KWLAANVSAE VAESTRIIYG GSVTGANCKE LAAKPDVDGF LVGGASLKPE 250 FIDIINSATV KSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cytosolic triosephosphate isomerase is a single gene in rice." Xu Y., Hall T.C. Plant Physiol. 101:683-687(1993) [PubMed: 8278510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lemont. Tissue: Root.
[2]	"Nuclear gene encoding cytosolic triosephosphate isomerase from rice (Oryza sativa L.)." Xu Y., Harris-Haller L.W., McCollum J.C., Hardin S.H., Hall T.C. Plant Physiol. 102:697-697(1993) [PubMed: 8108523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Gulfmont. Tissue: Leaf.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Strain: cv. Nipponbare. Tissue: Anther, Embryo and Stem.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M87064 mRNA. Translation: AAA18541.1. L04967 Genomic DNA. Translation: AAB63603.1. Frameshift. AP002872 Genomic DNA. Translation: BAB21144.1. AP002540 Genomic DNA. Translation: BAB43989.1.
PIR	JQ2255.
RefSeq	NP_001042016.1.
UniGene	Os.14506
3D structure databases
ModBase	Search...
2-D gel databases
ANU-2DPAGE	P48494.
Genome annotation databases
GeneID	4325211.
KEGG	osa:4325211.
Organism-specific databases
Gramene	P48494.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ORYSJ

Accession

Primary (citable) accession number: P48494
Secondary accession number(s): Q7F7B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)