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P48491 (TPIS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Triosephosphate isomerase, cytosolic
Short name=TIM
Short name=Triose-phosphate isomerase
EC=5.3.1.1
Gene names
Name:CTIMC
Ordered Locus Names:At3g55440
ORF Names:T22E16.100
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Probable.

Miscellaneous

In plants, there are two types of TPIS, cytosolic and plastid.

Sequence similarities

Belongs to the triosephosphate isomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Triosephosphate isomerase, cytosolic
PRO_0000090144

Sites

Active site961Electrophile By similarity
Active site1661Proton acceptor By similarity
Binding site101Substrate By similarity
Binding site121Substrate By similarity

Experimental info

Sequence conflict2511K → N in AAA03449. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48491 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 428023AFEB3269B4

FASTA25427,169
        10         20         30         40         50         60 
MARKFFVGGN WKCNGTAEEV KKIVNTLNEA QVPSQDVVEV VVSPPYVFLP LVKSTLRSDF 

        70         80         90        100        110        120 
FVAAQNCWVK KGGAFTGEVS AEMLVNLDIP WVILGHSERR AILNESSEFV GDKVAYALAQ 

       130        140        150        160        170        180 
GLKVIACVGE TLEEREAGST MDVVAAQTKA IADRVTNWSN VVIAYEPVWA IGTGKVASPA 

       190        200        210        220        230        240 
QAQEVHDELR KWLAKNVSAD VAATTRIIYG GSVNGGNCKE LGGQADVDGF LVGGASLKPE 

       250 
FIDIIKAAEV KKSA

« Hide

References

« Hide 'large scale' references
[1]"Structure and regulation of nuclear genes encoding chloroplast and cytosolic triosephosphate isomerase in Arabidopsis thaliana."
Shih M.-C.
Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Columbia.
Tissue: Leaf.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U02949 mRNA. Translation: AAA03449.1.
AL132975 Genomic DNA. Translation: CAB75902.1.
CP002686 Genomic DNA. Translation: AEE79384.1.
AF375426 mRNA. Translation: AAK53010.1.
AY074822 mRNA. Translation: AAL69518.1.
IPIIPI00549121.
PIRT47683.
T50646.
RefSeqNP_191104.1. NM_115402.3.
UniGeneAt.24563.
At.31873.
At.74726.

3D structure databases

ProteinModelPortalP48491.
SMRP48491. Positions 3-247.
ModBaseSearch...

Protein-protein interaction databases

IntActP48491. 2 interactions.
STRINGP48491.

2D gel databases

SWISS-2DPAGEP48491.
World-2DPAGE0003:P48491.

Proteomic databases

PRIDEP48491.
ProMEXP48491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G55440.1; AT3G55440.1; AT3G55440.
GeneID824710.
GenomeReviewsGene locus AT3G55440 in contig BA000014_GR.
KEGGath:AT3G55440.
NMPDRfig|3702.1.peg.16861.

Organism-specific databases

TAIRAt3g55440.

Phylogenomic databases

eggNOGKOG1643.
GeneTreeEPGT00070000029036.
HOGENOMHBG708281.
InParanoidP48491.
OMAISGQWPR.
PhylomeDBP48491.
ProtClustDBPLN02561.

Enzyme and pathway databases

BioCycARA:AT3G55440-MONOMER.
MetaCyc:AT3G55440-MONOMER.

Gene expression databases

ArrayExpressP48491.
GenevestigatorP48491.
GermOnlineAT3G55440. Arabidopsis thaliana.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01803.
PANTHERPTHR21139. Triophos_ismrse. 1 hit.
PfamPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMSSF51351. Triophos_ismrse. 1 hit.
TIGRFAMsTIGR00419. Tim. 1 hit.
PROSITEPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTPIS_ARATH
AccessionPrimary (citable) accession number: P48491
Secondary accession number(s): Q9M2T5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 27, 2001
Last modified: November 16, 2011
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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