P48488 (PP1_MEDSV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1
EC=3.1.3.16

Gene names

Name:

PP1

Organism

Medicago sativa subsp. varia (Alfalfa) (Medicago varia)

Taxonomic identifier

36902 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago › Medicago sativa

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Serine/threonine-protein phosphatase PP1		PRO_0000058807
Sites
	Active site	122	1	Proton donor By similarity
	Metal binding	61	1	Iron By similarity
	Metal binding	63	1	Iron By similarity
	Metal binding	89	1	Iron By similarity
	Metal binding	89	1	Manganese By similarity
	Metal binding	121	1	Manganese By similarity
	Metal binding	170	1	Manganese By similarity
	Metal binding	245	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48488 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: B2528E7BFCA0CA38	FASTA	321	36,248
	10 20 30 40 50 60 MDDTVLDDII KKLVSAKNGR TTKQVHLTEA DIRQLCTSAK EIFLSQPNLL ELEAPIKICG 70 80 90 100 110 120 DVHGQFSDLL RLFEYGGYPP EANYLFLGDY VDRGKQSIET ICLLLAYKIK YKENFFLLRG 130 140 150 160 170 180 NHECASINRI YGFYDECKRR YNVRLWKTFT DCFNCLPVAA LVDEKILCMH GGLSPELKNL 190 200 210 220 230 240 DQIRNIARPI DVPDHGLLCD LLWADPDKDL EGWGENDRGV SFTFGADKVV EFLEHHDLDL 250 260 270 280 290 300 ICRAHQVVED GYEFFAKRKL VTVFSAPNYC GEFDNAGAMM SVDDSLTCSF QILKSSDKKG 310 320 KVGFGNNSSR PGTPPHKGGK N

References

[1]	"Isolation and characterization of phosphoprotein phosphatase 1 from alfalfa." Pay A., Pirck M., Boerge L., Hirt H., Heberle-Bors E. Mol. Gen. Genet. 244:176-182(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80788 mRNA. Translation: CAA56766.1.
PIR	S46282.
3D structure databases
ProteinModelPortal	P48488.
SMR	P48488. Positions 6-305.
ModBase	Search...
Proteomic databases
PRIDE	P48488.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP1_MEDSV

Accession

Primary (citable) accession number: P48488

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program