P48487 (PP1_BRAOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1
EC=3.1.3.16

Gene names

Name:

PP1

Organism

Brassica oleracea (Wild cabbage)

Taxonomic identifier

3712 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Serine/threonine-protein phosphatase PP1		PRO_0000058806
Sites
	Active site	140	1	Proton donor By similarity
	Metal binding	79	1	Iron By similarity
	Metal binding	81	1	Iron By similarity
	Metal binding	107	1	Iron By similarity
	Metal binding	107	1	Manganese By similarity
	Metal binding	139	1	Manganese By similarity
	Metal binding	187	1	Manganese By similarity
	Metal binding	262	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48487 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 66D5131A3ECA4DF0	FASTA	316	35,902
	10 20 30 40 50 60 MAEKPAPEQE QEQTRAMEPA VLDDIIRRLV EFRNTRPGSG KQVHLSEGEI RQLCAVSKDI 70 80 90 100 110 120 FLQQPILLEL EAPIKICGDI HGQYSDLLRL FEYGGFPPDA NYLFLGDYVD RGKQSLETIC 130 140 150 160 170 180 FLLAYKIKYP ENFFLLRGNH ECASINRIYG FYDECKRRFN VRLWKIFTDC FNCLPVAALI 190 200 210 220 230 240 DRILCMHGGI SPELMSLDQI RSISRPLDIP DSGLVCDLLW SDPSGDVKGW GANDRGVSYT 250 260 270 280 290 300 FGADTVAEFL QKNDMDLICR AHQVVEDGYE FFAERQVVTV FSAPNYCGEF DNAGAMMSID 310 ESLMCSFQIL KPSEKK

References

[1]	"Molecular characterization of type 1 serine/threonine phosphatases from Brassica oleracea." Rundle S.J., Nasrallah J.B. Plant Mol. Biol. 20:367-375(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Flower.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63558 mRNA. Translation: CAA45119.1.
PIR	S26225.
3D structure databases
ProteinModelPortal	P48487.
SMR	P48487. Positions 22-316.
ModBase	Search...
Proteomic databases
PRIDE	P48487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP1_BRAOL

Accession

Primary (citable) accession number: P48487

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program