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Protein

Serine/threonine-protein phosphatase PP1 isozyme 6

Gene

TOPP6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Phosphatase activity is strongly reduced by the protein phosphatase inhibitor 2 (I-2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Manganese 1By similarity1
Metal bindingi63Manganese 1By similarity1
Metal bindingi89Manganese 1By similarity1
Metal bindingi89Manganese 2By similarity1
Metal bindingi121Manganese 2By similarity1
Active sitei122Proton donorBy similarity1
Metal bindingi170Manganese 2By similarity1
Metal bindingi245Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 6Curated (EC:3.1.3.161 Publication)
Alternative name(s):
Type one protein phosphatase 61 Publication
Gene namesi
Name:TOPP61 Publication
Synonyms:PP1BGImported, TOPP71 Publication
Ordered Locus Names:At4g11240Imported
ORF Names:F8L21.30Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G11240.
TAIRilocus:2128258. AT4G11240.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588021 – 322Serine/threonine-protein phosphatase PP1 isozyme 6Add BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP48486.
PRIDEiP48486.

PTM databases

iPTMnetiP48486.

Expressioni

Tissue specificityi

Strongly up-regulated within developing flowers, especially in the tapetum, the developing and mature pollen and in the ovaries.

Gene expression databases

ExpressionAtlasiP48486. baseline and differential.
GenevisibleiP48486. AT.

Interactioni

Protein-protein interaction databases

BioGridi12025. 3 interactors.
IntActiP48486. 2 interactors.
STRINGi3702.AT4G11240.1.

Structurei

3D structure databases

ProteinModelPortaliP48486.
SMRiP48486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000172697.
InParanoidiP48486.
KOiK06269.
OMAiQRGYEFF.
OrthoDBiEOG09360F05.
PhylomeDBiP48486.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P48486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDETLLDDII RRLLATNNGR TVKQAQITET EIRQLCLASK EVFLSQPNLL
60 70 80 90 100
ELEAPIKICG DVHGQFPDLL RLFEYGGYPP AANYLFLGDY VDRGKQSIET
110 120 130 140 150
ICLLLAYKVK YKFNFFLLRG NHECASINRV YGFYDECKRR YNVRLWKTFT
160 170 180 190 200
ECFNCLPVSA LIDDKILCMH GGLSPDIKSL DDIRRIPRPI DVPDQGILCD
210 220 230 240 250
LLWADPDREI QGWGENDRGV SYTFGADKVA EFLQTHDLDL ICRAHQVVED
260 270 280 290 300
GYEFFAKRQL VTIFSAPNYC GEFDNAGALM SVDDSLTCSF QILKASEKKG
310 320
RFGFNNNVPR PGTPPHKGGK GR
Length:322
Mass (Da):36,567
Last modified:May 29, 2007 - v2
Checksum:iB2320EB8E472AEDF
GO

Sequence cautioni

The sequence CAB51408 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81225 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248V → D in CAA86339 (PubMed:7773310).Curated1
Sequence conflicti248V → D in AAC39460 (PubMed:9617814).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46253 mRNA. Translation: CAA86339.1.
U80921 Genomic DNA. Translation: AAC39460.1.
AL096882 Genomic DNA. Translation: CAB51408.1. Sequence problems.
AL161531 Genomic DNA. Translation: CAB81225.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82988.1.
AY090365 mRNA. Translation: AAL91268.1.
AY122904 mRNA. Translation: AAM67437.1.
AY086060 mRNA. Translation: AAM63269.1.
PIRiT13015.
RefSeqiNP_567375.1. NM_117195.5.
UniGeneiAt.3651.

Genome annotation databases

EnsemblPlantsiAT4G11240.1; AT4G11240.1; AT4G11240.
GeneIDi826726.
GrameneiAT4G11240.1; AT4G11240.1; AT4G11240.
KEGGiath:AT4G11240.

Similar proteinsi

Entry informationi

Entry nameiPP16_ARATH
AccessioniPrimary (citable) accession number: P48486
Secondary accession number(s): Q8RX64, Q9SUT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 29, 2007
Last modified: November 22, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families