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Protein

Serine/threonine-protein phosphatase PP1 isozyme 5

Gene

TOPP5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Phosphatase activity is strongly reduced by the protein phosphatase inhibitor 2 (I-2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Manganese 1By similarity1
Metal bindingi72Manganese 1By similarity1
Metal bindingi98Manganese 1By similarity1
Metal bindingi98Manganese 2By similarity1
Metal bindingi130Manganese 2By similarity1
Active sitei131Proton donorBy similarity1
Metal bindingi179Manganese 2By similarity1
Metal bindingi254Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 5Curated (EC:3.1.3.161 Publication)
Alternative name(s):
Type one protein phosphatase 51 Publication
Gene namesi
Name:TOPP51 Publication
Ordered Locus Names:At3g46820Imported
ORF Names:T6H20.150Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G46820
TAIRilocus:2102762 AT3G46820

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000588012 – 312Serine/threonine-protein phosphatase PP1 isozyme 5Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP48485
PRIDEiP48485

PTM databases

iPTMnetiP48485

Expressioni

Gene expression databases

ExpressionAtlasiP48485 baseline and differential
GenevisibleiP48485 AT

Interactioni

Protein-protein interaction databases

BioGridi9155, 3 interactors
STRINGi3702.AT3G46820.1

Structurei

3D structure databases

ProteinModelPortaliP48485
SMRiP48485
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0374 Eukaryota
COG0639 LUCA
HOGENOMiHOG000172697
InParanoidiP48485
KOiK06269
OMAiAGTKQAM
OrthoDBiEOG09360F05
PhylomeDBiP48485

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
IPR031675 STPPase_N
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF16891 STPPase_N, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQGQGSMD PAVLDDIIRR LLDYRNPKAG TKQAMLNDSE IRQLCFVSRE
60 70 80 90 100
IFLQQPCLLE LAAPVKICGD IHGQYSDLLR LFEYGGFPPA ANYLFLGDYV
110 120 130 140 150
DRGKQSLETI CLLLAYKIKY PENFFLLRGN HECASINRIY GFYDECKRRF
160 170 180 190 200
NVKLWKVFTD TFNCLPVAAV IDEKILCMHG GLSPELINVE QIKNIERPTD
210 220 230 240 250
VPDAGLLCDL LWSDPSKDVK GWGMNDRGVS YTFGADKVAE FLIKNDMDLV
260 270 280 290 300
CRAHQVVEDG YEFFADRQLV TMFSAPNYCG EFDNAGALMS VDESLMCSFQ
310
ILKPVDRRSR FF
Length:312
Mass (Da):35,519
Last modified:February 1, 1996 - v1
Checksum:i937084D580708A68
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245 – 246ND → IP in CAA78152 (PubMed:8220477).Curated2
Sequence conflicti288L → M in CAA78152 (PubMed:8220477).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93412 mRNA Translation: AAA32840.1
AL096859 Genomic DNA Translation: CAB51183.1
CP002686 Genomic DNA Translation: AEE78208.1
BT024715 mRNA Translation: ABD59053.1
AK220755 mRNA Translation: BAD93940.1
AK227072 mRNA Translation: BAE99129.1
Z12162 mRNA Translation: CAA78152.1
PIRiS25532
S31089
RefSeqiNP_190266.1, NM_114549.4
UniGeneiAt.311

Genome annotation databases

EnsemblPlantsiAT3G46820.1; AT3G46820.1; AT3G46820
GeneIDi823835
GrameneiAT3G46820.1; AT3G46820.1; AT3G46820
KEGGiath:AT3G46820

Similar proteinsi

Entry informationi

Entry nameiPP15_ARATH
AccessioniPrimary (citable) accession number: P48485
Secondary accession number(s): Q29Q41, Q570F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health