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P48485 (PP15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP1 isozyme 5

EC=3.1.3.16
Gene names
Name:TOPP5
Ordered Locus Names:At3g46820
ORF Names:T6H20.150
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 312311Serine/threonine-protein phosphatase PP1 isozyme 5
PRO_0000058801

Sites

Active site1311Proton donor By similarity
Metal binding701Manganese 1 By similarity
Metal binding721Manganese 1 By similarity
Metal binding981Manganese 1 By similarity
Metal binding981Manganese 2 By similarity
Metal binding1301Manganese 2 By similarity
Metal binding1791Manganese 2 By similarity
Metal binding2541Manganese 2 By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.8

Experimental info

Sequence conflict245 – 2462ND → IP in CAA78152. Ref.6
Sequence conflict2881L → M in CAA78152. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P48485 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 937084D580708A68

FASTA31235,519
        10         20         30         40         50         60 
MAQQGQGSMD PAVLDDIIRR LLDYRNPKAG TKQAMLNDSE IRQLCFVSRE IFLQQPCLLE 

        70         80         90        100        110        120 
LAAPVKICGD IHGQYSDLLR LFEYGGFPPA ANYLFLGDYV DRGKQSLETI CLLLAYKIKY 

       130        140        150        160        170        180 
PENFFLLRGN HECASINRIY GFYDECKRRF NVKLWKVFTD TFNCLPVAAV IDEKILCMHG 

       190        200        210        220        230        240 
GLSPELINVE QIKNIERPTD VPDAGLLCDL LWSDPSKDVK GWGMNDRGVS YTFGADKVAE 

       250        260        270        280        290        300 
FLIKNDMDLV CRAHQVVEDG YEFFADRQLV TMFSAPNYCG EFDNAGALMS VDESLMCSFQ 

       310 
ILKPVDRRSR FF 

« Hide

References

« Hide 'large scale' references
[1]"Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana."
Smith R.D., Walker J.C.
Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Ecker J.R.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"A protein phosphatase 1 from Arabidopsis thaliana restores temperature sensitivity of a Schizosaccharomyces pombe cdc25ts/wee1-double mutant."
Ferreira P.C.G., Hemerly A.S., van Montagu M., Inze D.
Plant J. 4:81-87(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 245-312.
[7]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93412 mRNA. Translation: AAA32840.1.
AL096859 Genomic DNA. Translation: CAB51183.1.
CP002686 Genomic DNA. Translation: AEE78208.1.
BT024715 mRNA. Translation: ABD59053.1.
AK220755 mRNA. Translation: BAD93940.1.
AK227072 mRNA. Translation: BAE99129.1.
Z12162 mRNA. Translation: CAA78152.1.
PIRS25532.
S31089.
RefSeqNP_190266.1. NM_114549.3.
UniGeneAt.311.

3D structure databases

ProteinModelPortalP48485.
SMRP48485. Positions 14-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8060796.

Proteomic databases

PaxDbP48485.
PRIDEP48485.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G46820.1; AT3G46820.1; AT3G46820.
GeneID823835.
KEGGath:AT3G46820.

Organism-specific databases

TAIRAT3G46820.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172697.
InParanoidP48485.
KOK06269.
OMAQIKNIER.
PhylomeDBP48485.

Enzyme and pathway databases

BioCycARA:AT3G46820-MONOMER.

Gene expression databases

GenevestigatorP48485.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP15_ARATH
AccessionPrimary (citable) accession number: P48485
Secondary accession number(s): Q29Q41, Q570F3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names