P48484 (PP14_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 4
EC=3.1.3.16

Gene names

Name:	TOPP4
Ordered Locus Names:	At2g39840
ORF Names:	T5I7.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Traceable author statement Ref.1. Source: TAIR
Cellular_component	protein phosphatase type 1 complex Inferred from sequence or structural similarity Ref.1. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 21222654. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Serine/threonine-protein phosphatase PP1 isozyme 4		PRO_0000058800
Sites
	Active site	135	1	Proton donor By similarity
	Metal binding	74	1	Iron By similarity
	Metal binding	76	1	Iron By similarity
	Metal binding	102	1	Iron By similarity
	Metal binding	102	1	Manganese By similarity
	Metal binding	134	1	Manganese By similarity
	Metal binding	183	1	Manganese By similarity
	Metal binding	258	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48484 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9E96830AB996FFB3	FASTA	321	36,372
	10 20 30 40 50 60 MATTTTTQGQ QTAIDSAVLD DIIRRLTEVR LARPGKQVQL SEAEIKQLCT TARDIFLQQP 70 80 90 100 110 120 NLLELEAPIK ICGDIHGQYS DLLRLFEYGG FPPSANYLFL GDYVDRGKQS LETICLLLAY 130 140 150 160 170 180 KIKYPGNFFL LRGNHECASI NRIYGFYDEC KRRFNVRVWK VFTDCFNCLP VAALIDDKIL 190 200 210 220 230 240 CMHGGLSPDL DHLDEIRNLP RPTMIPDTGL LCDLLWSDPG KDVKGWGMND RGVSYTFGPD 250 260 270 280 290 300 KVSEFLTKHD LDLVCRAHQV VEDGYEFFAD RQLVTVFSAP NYCGEFDNAG AMMSVDENLM 310 320 CSFQILKPAE KKTKFMMSTK I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana." Smith R.D., Walker J.C. Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M93411 mRNA. Translation: AAA32839.1. AC003000 Genomic DNA. Translation: AAB87136.1. CP002685 Genomic DNA. Translation: AEC09737.1. BT026118 mRNA. Translation: ABG48474.1. AK229028 mRNA. Translation: BAF00914.1.
IPI	IPI00532673.
PIR	S31088.
RefSeq	NP_181514.1. NM_129543.2.
UniGene	At.310.
3D structure databases
ProteinModelPortal	P48484.
SMR	P48484. Positions 19-309.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G39840.1-P.
Proteomic databases
PaxDb	P48484.
PRIDE	P48484.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G39840.1; AT2G39840.1; AT2G39840.
GeneID	818571.
KEGG	ath:AT2G39840.
Organism-specific databases
TAIR	At2g39840.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172697.
InParanoid	P48484.
KO	K06269.
OMA	PKADSAN.
PhylomeDB	P48484.
ProtClustDB	CLSN2683376.
Gene expression databases
ArrayExpress	P48484.
Genevestigator	P48484.
GermOnline	AT2G39840. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP14_ARATH

Accession

Primary (citable) accession number: P48484
Secondary accession number(s): Q147H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program