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P48484

- PP14_ARATH

UniProt

P48484 - PP14_ARATH

Protein

Serine/threonine-protein phosphatase PP1 isozyme 4

Gene

TOPP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi74 – 741Manganese 1By similarity
    Metal bindingi76 – 761Manganese 1By similarity
    Metal bindingi102 – 1021Manganese 1By similarity
    Metal bindingi102 – 1021Manganese 2By similarity
    Metal bindingi134 – 1341Manganese 2By similarity
    Active sitei135 – 1351Proton donorBy similarity
    Metal bindingi183 – 1831Manganese 2By similarity
    Metal bindingi258 – 2581Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. protein dephosphorylation Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G39840-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP1 isozyme 4 (EC:3.1.3.16)
    Gene namesi
    Name:TOPP4
    Ordered Locus Names:At2g39840
    ORF Names:T5I7.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G39840.

    Subcellular locationi

    GO - Cellular componenti

    1. protein phosphatase type 1 complex Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 321320Serine/threonine-protein phosphatase PP1 isozyme 4PRO_0000058800Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP48484.
    PRIDEiP48484.

    Expressioni

    Gene expression databases

    ArrayExpressiP48484.
    GenevestigatoriP48484.

    Interactioni

    Protein-protein interaction databases

    BioGridi3909. 2 interactions.
    STRINGi3702.AT2G39840.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP48484.
    SMRiP48484. Positions 19-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172697.
    InParanoidiP48484.
    KOiK06269.
    OMAiDEKICCH.
    PhylomeDBiP48484.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48484-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTTTTQGQ QTAIDSAVLD DIIRRLTEVR LARPGKQVQL SEAEIKQLCT    50
    TARDIFLQQP NLLELEAPIK ICGDIHGQYS DLLRLFEYGG FPPSANYLFL 100
    GDYVDRGKQS LETICLLLAY KIKYPGNFFL LRGNHECASI NRIYGFYDEC 150
    KRRFNVRVWK VFTDCFNCLP VAALIDDKIL CMHGGLSPDL DHLDEIRNLP 200
    RPTMIPDTGL LCDLLWSDPG KDVKGWGMND RGVSYTFGPD KVSEFLTKHD 250
    LDLVCRAHQV VEDGYEFFAD RQLVTVFSAP NYCGEFDNAG AMMSVDENLM 300
    CSFQILKPAE KKTKFMMSTK I 321
    Length:321
    Mass (Da):36,372
    Last modified:February 1, 1996 - v1
    Checksum:i9E96830AB996FFB3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93411 mRNA. Translation: AAA32839.1.
    AC003000 Genomic DNA. Translation: AAB87136.1.
    CP002685 Genomic DNA. Translation: AEC09737.1.
    BT026118 mRNA. Translation: ABG48474.1.
    AK229028 mRNA. Translation: BAF00914.1.
    PIRiS31088.
    RefSeqiNP_181514.1. NM_129543.2.
    UniGeneiAt.310.

    Genome annotation databases

    EnsemblPlantsiAT2G39840.1; AT2G39840.1; AT2G39840.
    GeneIDi818571.
    KEGGiath:AT2G39840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93411 mRNA. Translation: AAA32839.1 .
    AC003000 Genomic DNA. Translation: AAB87136.1 .
    CP002685 Genomic DNA. Translation: AEC09737.1 .
    BT026118 mRNA. Translation: ABG48474.1 .
    AK229028 mRNA. Translation: BAF00914.1 .
    PIRi S31088.
    RefSeqi NP_181514.1. NM_129543.2.
    UniGenei At.310.

    3D structure databases

    ProteinModelPortali P48484.
    SMRi P48484. Positions 19-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 3909. 2 interactions.
    STRINGi 3702.AT2G39840.1-P.

    Proteomic databases

    PaxDbi P48484.
    PRIDEi P48484.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G39840.1 ; AT2G39840.1 ; AT2G39840 .
    GeneIDi 818571.
    KEGGi ath:AT2G39840.

    Organism-specific databases

    TAIRi AT2G39840.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172697.
    InParanoidi P48484.
    KOi K06269.
    OMAi DEKICCH.
    PhylomeDBi P48484.

    Enzyme and pathway databases

    BioCyci ARA:AT2G39840-MONOMER.

    Gene expression databases

    ArrayExpressi P48484.
    Genevestigatori P48484.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana."
      Smith R.D., Walker J.C.
      Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPP14_ARATH
    AccessioniPrimary (citable) accession number: P48484
    Secondary accession number(s): Q147H5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3