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Protein

Serine/threonine-protein phosphatase PP1 isozyme 4

Gene

TOPP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Manganese 1By similarity
Metal bindingi76 – 761Manganese 1By similarity
Metal bindingi102 – 1021Manganese 1By similarity
Metal bindingi102 – 1021Manganese 2By similarity
Metal bindingi134 – 1341Manganese 2By similarity
Active sitei135 – 1351Proton donorBy similarity
Metal bindingi183 – 1831Manganese 2By similarity
Metal bindingi258 – 2581Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. protein dephosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G39840-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 4 (EC:3.1.3.16)
Gene namesi
Name:TOPP4
Ordered Locus Names:At2g39840
ORF Names:T5I7.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G39840.

Subcellular locationi

GO - Cellular componenti

  1. protein phosphatase type 1 complex Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 321320Serine/threonine-protein phosphatase PP1 isozyme 4PRO_0000058800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP48484.
PRIDEiP48484.

Expressioni

Gene expression databases

ExpressionAtlasiP48484. baseline and differential.
GenevestigatoriP48484.

Interactioni

Protein-protein interaction databases

BioGridi3909. 2 interactions.
STRINGi3702.AT2G39840.1-P.

Structurei

3D structure databases

ProteinModelPortaliP48484.
SMRiP48484. Positions 19-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172697.
InParanoidiP48484.
KOiK06269.
OMAiCVSSRDI.
PhylomeDBiP48484.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTTTTQGQ QTAIDSAVLD DIIRRLTEVR LARPGKQVQL SEAEIKQLCT
60 70 80 90 100
TARDIFLQQP NLLELEAPIK ICGDIHGQYS DLLRLFEYGG FPPSANYLFL
110 120 130 140 150
GDYVDRGKQS LETICLLLAY KIKYPGNFFL LRGNHECASI NRIYGFYDEC
160 170 180 190 200
KRRFNVRVWK VFTDCFNCLP VAALIDDKIL CMHGGLSPDL DHLDEIRNLP
210 220 230 240 250
RPTMIPDTGL LCDLLWSDPG KDVKGWGMND RGVSYTFGPD KVSEFLTKHD
260 270 280 290 300
LDLVCRAHQV VEDGYEFFAD RQLVTVFSAP NYCGEFDNAG AMMSVDENLM
310 320
CSFQILKPAE KKTKFMMSTK I
Length:321
Mass (Da):36,372
Last modified:February 1, 1996 - v1
Checksum:i9E96830AB996FFB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93411 mRNA. Translation: AAA32839.1.
AC003000 Genomic DNA. Translation: AAB87136.1.
CP002685 Genomic DNA. Translation: AEC09737.1.
BT026118 mRNA. Translation: ABG48474.1.
AK229028 mRNA. Translation: BAF00914.1.
PIRiS31088.
RefSeqiNP_181514.1. NM_129543.2.
UniGeneiAt.310.

Genome annotation databases

EnsemblPlantsiAT2G39840.1; AT2G39840.1; AT2G39840.
GeneIDi818571.
KEGGiath:AT2G39840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93411 mRNA. Translation: AAA32839.1.
AC003000 Genomic DNA. Translation: AAB87136.1.
CP002685 Genomic DNA. Translation: AEC09737.1.
BT026118 mRNA. Translation: ABG48474.1.
AK229028 mRNA. Translation: BAF00914.1.
PIRiS31088.
RefSeqiNP_181514.1. NM_129543.2.
UniGeneiAt.310.

3D structure databases

ProteinModelPortaliP48484.
SMRiP48484. Positions 19-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3909. 2 interactions.
STRINGi3702.AT2G39840.1-P.

Proteomic databases

PaxDbiP48484.
PRIDEiP48484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G39840.1; AT2G39840.1; AT2G39840.
GeneIDi818571.
KEGGiath:AT2G39840.

Organism-specific databases

TAIRiAT2G39840.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172697.
InParanoidiP48484.
KOiK06269.
OMAiCVSSRDI.
PhylomeDBiP48484.

Enzyme and pathway databases

BioCyciARA:AT2G39840-MONOMER.

Miscellaneous databases

PROiP48484.

Gene expression databases

ExpressionAtlasiP48484. baseline and differential.
GenevestigatoriP48484.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana."
    Smith R.D., Walker J.C.
    Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP14_ARATH
AccessioniPrimary (citable) accession number: P48484
Secondary accession number(s): Q147H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 29, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.