Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase PP1 isozyme 4

Gene

TOPP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro (PubMed:21222654). Acts as positive regulator in the gibberellin (GA) signaling pathway to regulate plant growth and development. Promotes the GA-induced and proteasomal-dependent degradation of the DELLA proteins RGA and GAI by directly binding and dephosphorylating these proteins (PubMed:25010794). Involved in the regulation of phytochrome B (phyB) signaling pathway that controls photomorphogenesis. Promotes the proteasomal-dependent degradation of PIF5 factor by directly binding and dephosphorylating this protein (PubMed:26704640). Involved in the regulation of pavement cell (PC) interdigitation by modulating the auxin efflux carrier PIN1 polarity and endocytic trafficking. Regulates PIN1 polar targeting through direct binding and dephosphorylation. Acts antagonistically with PID in regulating PC development (PubMed:25560878).4 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Phosphatase activity is strongly reduced by the protein phosphatase inhibitor 2 (I-2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74Manganese 1By similarity1
Metal bindingi76Manganese 1By similarity1
Metal bindingi102Manganese 1By similarity1
Metal bindingi102Manganese 2By similarity1
Metal bindingi134Manganese 2By similarity1
Active sitei135Proton donorBy similarity1
Metal bindingi183Manganese 2By similarity1
Metal bindingi258Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • protein dephosphorylation Source: TAIR
  • protein phosphorylation Source: TAIR
  • red light signaling pathway Source: TAIR
  • regulation of growth Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processGrowth regulation
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 4Curated (EC:3.1.3.161 Publication)
Alternative name(s):
Type one protein phosphatase 41 Publication
Gene namesi
Name:TOPP41 Publication
Ordered Locus Names:At2g39840Imported
ORF Names:T5I7.14Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G39840.
TAIRilocus:2063942. AT2G39840.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Dwarf phenotype and decreased fertility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000588002 – 321Serine/threonine-protein phosphatase PP1 isozyme 4Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP48484.
PRIDEiP48484.

PTM databases

iPTMnetiP48484.

Expressioni

Tissue specificityi

Expressed in the vasculature of roots and cotyledons, tips of leaves, guard cells, bases of trichomes, pistils and stamen filaments.1 Publication

Gene expression databases

ExpressionAtlasiP48484. baseline and differential.
GenevisibleiP48484. AT.

Interactioni

Subunit structurei

Interacts with the DELLA proteins RGA and GAI (PubMed:25010794). Interacts with PIF3 and PIF5 (PubMed:26704640). Interacts with the auxin efflux carrier PIN1 (PubMed:25560878).3 Publications

Protein-protein interaction databases

BioGridi3909. 7 interactors.
STRINGi3702.AT2G39840.1.

Structurei

3D structure databases

ProteinModelPortaliP48484.
SMRiP48484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000172697.
InParanoidiP48484.
KOiK06269.
OMAiQRGYEFF.
OrthoDBiEOG09360F05.
PhylomeDBiP48484.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTTTTQGQ QTAIDSAVLD DIIRRLTEVR LARPGKQVQL SEAEIKQLCT
60 70 80 90 100
TARDIFLQQP NLLELEAPIK ICGDIHGQYS DLLRLFEYGG FPPSANYLFL
110 120 130 140 150
GDYVDRGKQS LETICLLLAY KIKYPGNFFL LRGNHECASI NRIYGFYDEC
160 170 180 190 200
KRRFNVRVWK VFTDCFNCLP VAALIDDKIL CMHGGLSPDL DHLDEIRNLP
210 220 230 240 250
RPTMIPDTGL LCDLLWSDPG KDVKGWGMND RGVSYTFGPD KVSEFLTKHD
260 270 280 290 300
LDLVCRAHQV VEDGYEFFAD RQLVTVFSAP NYCGEFDNAG AMMSVDENLM
310 320
CSFQILKPAE KKTKFMMSTK I
Length:321
Mass (Da):36,372
Last modified:February 1, 1996 - v1
Checksum:i9E96830AB996FFB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93411 mRNA. Translation: AAA32839.1.
AC003000 Genomic DNA. Translation: AAB87136.1.
CP002685 Genomic DNA. Translation: AEC09737.1.
BT026118 mRNA. Translation: ABG48474.1.
AK229028 mRNA. Translation: BAF00914.1.
PIRiS31088.
RefSeqiNP_181514.1. NM_129543.3.
UniGeneiAt.310.

Genome annotation databases

EnsemblPlantsiAT2G39840.1; AT2G39840.1; AT2G39840.
GeneIDi818571.
GrameneiAT2G39840.1; AT2G39840.1; AT2G39840.
KEGGiath:AT2G39840.

Similar proteinsi

Entry informationi

Entry nameiPP14_ARATH
AccessioniPrimary (citable) accession number: P48484
Secondary accession number(s): Q147H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome