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P48483

- PP13_ARATH

UniProt

P48483 - PP13_ARATH

Protein

Serine/threonine-protein phosphatase PP1 isozyme 3

Gene

TOPP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611Manganese 1By similarity
    Metal bindingi63 – 631Manganese 1By similarity
    Metal bindingi89 – 891Manganese 1By similarity
    Metal bindingi89 – 891Manganese 2By similarity
    Metal bindingi121 – 1211Manganese 2By similarity
    Active sitei122 – 1221Proton donorBy similarity
    Metal bindingi170 – 1701Manganese 2By similarity
    Metal bindingi245 – 2451Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. protein dephosphorylation Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G64040-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP1 isozyme 3 (EC:3.1.3.16)
    Gene namesi
    Name:TOPP3
    Ordered Locus Names:At1g64040
    ORF Names:F22C12.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G64040.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. nucleolus Source: TAIR
    3. nucleus Source: TAIR
    4. protein phosphatase type 1 complex Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322Serine/threonine-protein phosphatase PP1 isozyme 3PRO_0000058799Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP48483.
    PRIDEiP48483.

    Expressioni

    Gene expression databases

    GenevestigatoriP48483.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G64040.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP48483.
    SMRiP48483. Positions 6-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172697.
    InParanoidiP48483.
    KOiK06269.
    OMAiDECKKRY.
    PhylomeDBiP48483.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48483-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDSVVDDVI KRLLGAKNGK TTKQVQLTEA EIKHLCSTAK QIFLTQPNLL    50
    ELEAPIKICG DTHGQFSDLL RLFEYGGYPP AANYLFLGDY VDRGKQSVET 100
    ICLLLAYKIK YKENFFLLRG NHECASINRI YGFYDECKKR YSVRVWKIFT 150
    DCFNCLPVAA LIDEKILCMH GGLSPELKHL DEIRNIPRPA DIPDHGLLCD 200
    LLWSDPDKDI EGWGENDRGV SYTFGADKVE EFLQTHDLDL ICRAHQVVED 250
    GYEFFANRQL VTIFSAPNYC GEFDNAGAMM SVDDSLTCSF QILKASEKKG 300
    NFGFGKNAGR RGTPPRKGGG KG 322
    Length:322
    Mass (Da):36,215
    Last modified:February 1, 1996 - v1
    Checksum:i644A70BB9F63215C
    GO

    Sequence cautioni

    The sequence AAF24566.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93410 mRNA. Translation: AAA32838.1.
    AC007764 Genomic DNA. Translation: AAF24566.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34183.1.
    AY081338 mRNA. Translation: AAL91227.1.
    AY128830 mRNA. Translation: AAM91230.1.
    PIRiC96665.
    S31087.
    RefSeqiNP_176587.1. NM_105077.3.
    UniGeneiAt.309.

    Genome annotation databases

    EnsemblPlantsiAT1G64040.1; AT1G64040.1; AT1G64040.
    GeneIDi842708.
    KEGGiath:AT1G64040.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93410 mRNA. Translation: AAA32838.1 .
    AC007764 Genomic DNA. Translation: AAF24566.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34183.1 .
    AY081338 mRNA. Translation: AAL91227.1 .
    AY128830 mRNA. Translation: AAM91230.1 .
    PIRi C96665.
    S31087.
    RefSeqi NP_176587.1. NM_105077.3.
    UniGenei At.309.

    3D structure databases

    ProteinModelPortali P48483.
    SMRi P48483. Positions 6-294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G64040.1-P.

    Proteomic databases

    PaxDbi P48483.
    PRIDEi P48483.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G64040.1 ; AT1G64040.1 ; AT1G64040 .
    GeneIDi 842708.
    KEGGi ath:AT1G64040.

    Organism-specific databases

    TAIRi AT1G64040.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172697.
    InParanoidi P48483.
    KOi K06269.
    OMAi DECKKRY.
    PhylomeDBi P48483.

    Enzyme and pathway databases

    BioCyci ARA:AT1G64040-MONOMER.

    Gene expression databases

    Genevestigatori P48483.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana."
      Smith R.D., Walker J.C.
      Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPP13_ARATH
    AccessioniPrimary (citable) accession number: P48483
    Secondary accession number(s): Q9SH54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3