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Protein

Serine/threonine-protein phosphatase PP1 isozyme 2

Gene

TOPP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Phosphatase activity is strongly reduced by the protein phosphatase inhibitor 2 (I-2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Manganese 1By similarity1
Metal bindingi72Manganese 1By similarity1
Metal bindingi98Manganese 1By similarity1
Metal bindingi98Manganese 2By similarity1
Metal bindingi130Manganese 2By similarity1
Active sitei131Proton donorBy similarity1
Metal bindingi179Manganese 2By similarity1
Metal bindingi254Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • protein dephosphorylation Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 2Curated (EC:3.1.3.161 Publication)
Alternative name(s):
Type one protein phosphatase 21 Publication
Gene namesi
Name:TOPP21 Publication
Ordered Locus Names:At5g59160Imported
ORF Names:MNC17.7Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G59160.
TAIRilocus:2168484. AT5G59160.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000587982 – 312Serine/threonine-protein phosphatase PP1 isozyme 2Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP48482.
PRIDEiP48482.

PTM databases

iPTMnetiP48482.

Expressioni

Gene expression databases

ExpressionAtlasiP48482. baseline and differential.
GenevisibleiP48482. AT.

Interactioni

Subunit structurei

Interacts with SRK2D/SNRK2.2 and SRK2E/SNRK2.6.1 Publication

Protein-protein interaction databases

BioGridi21278. 1 interactor.
STRINGi3702.AT5G59160.1.

Structurei

3D structure databases

ProteinModelPortaliP48482.
SMRiP48482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000172697.
InParanoidiP48482.
KOiK06269.
OMAiWGENDER.
OrthoDBiEOG09360F05.
PhylomeDBiP48482.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQGQGSMD PAALDDIIRR LLDYRNPKPG TKQAMLNESE IRQLCIVSRE
60 70 80 90 100
IFLQQPNLLE LEAPIKICGD IHGQYSDLLR LFEYGGFPPT ANYLFLGDYV
110 120 130 140 150
DRGKQSLETI CLLLAYKIKY PENFFLLRGN HECASINRIY GFYDECKRRF
160 170 180 190 200
SVRLWKVFTD SFNCLPVAAV IDDKILCMHG GLSPDLTNVE QIKNIKRPTD
210 220 230 240 250
VPDSGLLCDL LWSDPSKDVK GWGMNDRGVS YTFGPDKVAE FLIKNDMDLI
260 270 280 290 300
CRAHQVVEDG YEFFADRQLV TIFSAPNYCG EFDNAGAMMS VDESLMCSFQ
310
ILKPADRKPR FL
Length:312
Mass (Da):35,532
Last modified:February 1, 1996 - v1
Checksum:iDF71C23B5CB53CC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181 – 182GL → AI in CAA78153 (PubMed:8220477).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93409 mRNA. Translation: AAA32837.1.
Z12163 mRNA. Translation: CAA78153.1.
AB016890 Genomic DNA. Translation: BAB09762.1.
CP002688 Genomic DNA. Translation: AED97150.1.
CP002688 Genomic DNA. Translation: AED97151.1.
CP002688 Genomic DNA. Translation: AED97152.1.
AY042840 mRNA. Translation: AAK68780.1.
BT002401 mRNA. Translation: AAO00761.1.
BT024861 mRNA. Translation: ABD65592.1.
PIRiS24264.
S31086.
RefSeqiNP_001032103.1. NM_001037026.1.
NP_200724.1. NM_125306.2.
NP_851218.1. NM_180887.5.
UniGeneiAt.20932.
At.311.

Genome annotation databases

EnsemblPlantsiAT5G59160.1; AT5G59160.1; AT5G59160.
AT5G59160.2; AT5G59160.2; AT5G59160.
AT5G59160.3; AT5G59160.3; AT5G59160.
GeneIDi836034.
GrameneiAT5G59160.1; AT5G59160.1; AT5G59160.
AT5G59160.2; AT5G59160.2; AT5G59160.
AT5G59160.3; AT5G59160.3; AT5G59160.
KEGGiath:AT5G59160.

Similar proteinsi

Entry informationi

Entry nameiPP12_ARATH
AccessioniPrimary (citable) accession number: P48482
Secondary accession number(s): Q24JM8, Q94B57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 22, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families