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P48482 (PP12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP1 isozyme 2

EC=3.1.3.16
Gene names
Name:TOPP2
Ordered Locus Names:At5g59160
ORF Names:MNC17.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 312311Serine/threonine-protein phosphatase PP1 isozyme 2
PRO_0000058798

Sites

Active site1311Proton donor By similarity
Metal binding701Iron By similarity
Metal binding721Iron By similarity
Metal binding981Iron By similarity
Metal binding981Manganese By similarity
Metal binding1301Manganese By similarity
Metal binding1791Manganese By similarity
Metal binding2541Manganese By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.8

Experimental info

Sequence conflict181 – 1822GL → AI in CAA78153. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P48482 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: DF71C23B5CB53CC2

FASTA31235,532
        10         20         30         40         50         60 
MAQQGQGSMD PAALDDIIRR LLDYRNPKPG TKQAMLNESE IRQLCIVSRE IFLQQPNLLE 

        70         80         90        100        110        120 
LEAPIKICGD IHGQYSDLLR LFEYGGFPPT ANYLFLGDYV DRGKQSLETI CLLLAYKIKY 

       130        140        150        160        170        180 
PENFFLLRGN HECASINRIY GFYDECKRRF SVRLWKVFTD SFNCLPVAAV IDDKILCMHG 

       190        200        210        220        230        240 
GLSPDLTNVE QIKNIKRPTD VPDSGLLCDL LWSDPSKDVK GWGMNDRGVS YTFGPDKVAE 

       250        260        270        280        290        300 
FLIKNDMDLI CRAHQVVEDG YEFFADRQLV TIFSAPNYCG EFDNAGAMMS VDESLMCSFQ 

       310 
ILKPADRKPR FL 

« Hide

References

« Hide 'large scale' references
[1]"Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana."
Smith R.D., Walker J.C.
Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A protein phosphatase 1 from Arabidopsis thaliana restores temperature sensitivity of a Schizosaccharomyces pombe cdc25ts/wee1-double mutant."
Ferreira P.C.G., Hemerly A.S., van Montagu M., Inze D.
Plant J. 4:81-87(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Arabidopsis ORF clones."
Kim C.J., Chen H., Shinn P., Ecker J.R.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93409 mRNA. Translation: AAA32837.1.
Z12163 mRNA. Translation: CAA78153.1.
AB016890 Genomic DNA. Translation: BAB09762.1.
CP002688 Genomic DNA. Translation: AED97150.1.
CP002688 Genomic DNA. Translation: AED97151.1.
CP002688 Genomic DNA. Translation: AED97152.1.
AY042840 mRNA. Translation: AAK68780.1.
BT002401 mRNA. Translation: AAO00761.1.
BT024861 mRNA. Translation: ABD65592.1.
PIRS24264.
S31086.
RefSeqNP_001032103.1. NM_001037026.1.
NP_200724.1. NM_125306.2.
NP_851218.1. NM_180887.4.
UniGeneAt.20932.
At.311.

3D structure databases

ProteinModelPortalP48482.
SMRP48482. Positions 14-305.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP48482.
PRIDEP48482.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G59160.1; AT5G59160.1; AT5G59160.
AT5G59160.2; AT5G59160.2; AT5G59160.
AT5G59160.3; AT5G59160.3; AT5G59160.
GeneID836034.
KEGGath:AT5G59160.

Organism-specific databases

TAIRAT5G59160.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172697.
InParanoidP48482.
KOK06269.
OMAYLSSACY.
PhylomeDBP48482.
ProtClustDBCLSN2685131.

Enzyme and pathway databases

BioCycARA:AT5G59160-MONOMER.
ARA:GQT-2764-MONOMER.
ARA:GQT-2765-MONOMER.

Gene expression databases

GenevestigatorP48482.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP12_ARATH
AccessionPrimary (citable) accession number: P48482
Secondary accession number(s): Q24JM8, Q94B57
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names