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P48482

- PP12_ARATH

UniProt

P48482 - PP12_ARATH

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Protein
Serine/threonine-protein phosphatase PP1 isozyme 2
Gene
TOPP2, At5g59160, MNC17.7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Manganese 1 By similarity
Metal bindingi72 – 721Manganese 1 By similarity
Metal bindingi98 – 981Manganese 1 By similarity
Metal bindingi98 – 981Manganese 2 By similarity
Metal bindingi130 – 1301Manganese 2 By similarity
Active sitei131 – 1311Proton donor By similarity
Metal bindingi179 – 1791Manganese 2 By similarity
Metal bindingi254 – 2541Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. protein dephosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G59160-MONOMER.
ARA:GQT-2764-MONOMER.
ARA:GQT-2765-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 2 (EC:3.1.3.16)
Gene namesi
Name:TOPP2
Ordered Locus Names:At5g59160
ORF Names:MNC17.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G59160.

Subcellular locationi

GO - Cellular componenti

  1. protein phosphatase type 1 complex Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 312311Serine/threonine-protein phosphatase PP1 isozyme 2
PRO_0000058798Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP48482.
PRIDEiP48482.

Expressioni

Gene expression databases

GenevestigatoriP48482.

Structurei

3D structure databases

ProteinModelPortaliP48482.
SMRiP48482. Positions 14-305.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172697.
InParanoidiP48482.
KOiK06269.
OMAiYLSSACY.
PhylomeDBiP48482.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48482-1 [UniParc]FASTAAdd to Basket

« Hide

MAQQGQGSMD PAALDDIIRR LLDYRNPKPG TKQAMLNESE IRQLCIVSRE    50
IFLQQPNLLE LEAPIKICGD IHGQYSDLLR LFEYGGFPPT ANYLFLGDYV 100
DRGKQSLETI CLLLAYKIKY PENFFLLRGN HECASINRIY GFYDECKRRF 150
SVRLWKVFTD SFNCLPVAAV IDDKILCMHG GLSPDLTNVE QIKNIKRPTD 200
VPDSGLLCDL LWSDPSKDVK GWGMNDRGVS YTFGPDKVAE FLIKNDMDLI 250
CRAHQVVEDG YEFFADRQLV TIFSAPNYCG EFDNAGAMMS VDESLMCSFQ 300
ILKPADRKPR FL 312
Length:312
Mass (Da):35,532
Last modified:February 1, 1996 - v1
Checksum:iDF71C23B5CB53CC2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1822GL → AI in CAA78153. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93409 mRNA. Translation: AAA32837.1.
Z12163 mRNA. Translation: CAA78153.1.
AB016890 Genomic DNA. Translation: BAB09762.1.
CP002688 Genomic DNA. Translation: AED97150.1.
CP002688 Genomic DNA. Translation: AED97151.1.
CP002688 Genomic DNA. Translation: AED97152.1.
AY042840 mRNA. Translation: AAK68780.1.
BT002401 mRNA. Translation: AAO00761.1.
BT024861 mRNA. Translation: ABD65592.1.
PIRiS24264.
S31086.
RefSeqiNP_001032103.1. NM_001037026.1.
NP_200724.1. NM_125306.2.
NP_851218.1. NM_180887.4.
UniGeneiAt.20932.
At.311.

Genome annotation databases

EnsemblPlantsiAT5G59160.1; AT5G59160.1; AT5G59160.
AT5G59160.2; AT5G59160.2; AT5G59160.
AT5G59160.3; AT5G59160.3; AT5G59160.
GeneIDi836034.
KEGGiath:AT5G59160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93409 mRNA. Translation: AAA32837.1 .
Z12163 mRNA. Translation: CAA78153.1 .
AB016890 Genomic DNA. Translation: BAB09762.1 .
CP002688 Genomic DNA. Translation: AED97150.1 .
CP002688 Genomic DNA. Translation: AED97151.1 .
CP002688 Genomic DNA. Translation: AED97152.1 .
AY042840 mRNA. Translation: AAK68780.1 .
BT002401 mRNA. Translation: AAO00761.1 .
BT024861 mRNA. Translation: ABD65592.1 .
PIRi S24264.
S31086.
RefSeqi NP_001032103.1. NM_001037026.1.
NP_200724.1. NM_125306.2.
NP_851218.1. NM_180887.4.
UniGenei At.20932.
At.311.

3D structure databases

ProteinModelPortali P48482.
SMRi P48482. Positions 14-305.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P48482.
PRIDEi P48482.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G59160.1 ; AT5G59160.1 ; AT5G59160 .
AT5G59160.2 ; AT5G59160.2 ; AT5G59160 .
AT5G59160.3 ; AT5G59160.3 ; AT5G59160 .
GeneIDi 836034.
KEGGi ath:AT5G59160.

Organism-specific databases

TAIRi AT5G59160.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172697.
InParanoidi P48482.
KOi K06269.
OMAi YLSSACY.
PhylomeDBi P48482.

Enzyme and pathway databases

BioCyci ARA:AT5G59160-MONOMER.
ARA:GQT-2764-MONOMER.
ARA:GQT-2765-MONOMER.

Gene expression databases

Genevestigatori P48482.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana."
    Smith R.D., Walker J.C.
    Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A protein phosphatase 1 from Arabidopsis thaliana restores temperature sensitivity of a Schizosaccharomyces pombe cdc25ts/wee1-double mutant."
    Ferreira P.C.G., Hemerly A.S., van Montagu M., Inze D.
    Plant J. 4:81-87(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Shinn P., Ecker J.R.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP12_ARATH
AccessioniPrimary (citable) accession number: P48482
Secondary accession number(s): Q24JM8, Q94B57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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