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P48464

- TYSY_SHIFL

UniProt

P48464 - TYSY_SHIFL

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Protein

Thymidylate synthase

Gene

thyA

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461UniRule annotation

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:SF2837, S3035
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Thymidylate synthasePRO_0000141016Add
BLAST

Proteomic databases

PaxDbiP48464.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198214.SF2837.

Structurei

3D structure databases

ProteinModelPortaliP48464.
SMRiP48464. Positions 1-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiWRSWATA.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48464-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC
60 70 80 90 100
HLRSIIHELL WFLQGDTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRA
110 120 130 140 150
WPTPDGRHID QITTVLNQLK NDPDSRRIIV SAWNVGELDK MALAPCHAFF
160 170 180 190 200
QFYVADGKLS CQLYQRSCDV FLGLPFNIAS YALLVHMMAQ QCDLEVGDFV
210 220 230 240 250
WTGGDTHLYS NHMDQTHLQL SREPRPLPKL IIKRKPESIF DYRFEDFEIE
260
GYDPHPGVKA PVAI
Length:264
Mass (Da):30,466
Last modified:January 10, 2003 - v2
Checksum:i0E6D88ED98D25FB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701A → G in AAP31579. (PubMed:7935051)Curated
Sequence conflicti82 – 821E → D in AAP31579. (PubMed:7935051)Curated
Sequence conflicti94 – 941Y → F in AAP31579. (PubMed:7935051)Curated
Sequence conflicti182 – 1821A → T in AAP31579. (PubMed:7935051)Curated
Sequence conflicti210 – 2101S → T in AAP31579. (PubMed:7935051)Curated
Sequence conflicti258 – 2581V → I in AAP31579. (PubMed:7935051)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S75211 mRNA. Translation: AAP31579.1.
AE005674 Genomic DNA. Translation: AAN44323.1.
AE014073 Genomic DNA. Translation: AAP18149.1.
RefSeqiNP_708616.1. NC_004337.2.
NP_838339.1. NC_004741.1.
WP_000816233.1. NZ_KL503836.1.

Genome annotation databases

EnsemblBacteriaiAAN44323; AAN44323; SF2837.
AAP18149; AAP18149; S3035.
GeneIDi1025814.
1079305.
KEGGisfl:SF2837.
sfx:S3035.
PATRICi18707758. VBIShiFle31049_3331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S75211 mRNA. Translation: AAP31579.1 .
AE005674 Genomic DNA. Translation: AAN44323.1 .
AE014073 Genomic DNA. Translation: AAP18149.1 .
RefSeqi NP_708616.1. NC_004337.2.
NP_838339.1. NC_004741.1.
WP_000816233.1. NZ_KL503836.1.

3D structure databases

ProteinModelPortali P48464.
SMRi P48464. Positions 1-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF2837.

Proteomic databases

PaxDbi P48464.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN44323 ; AAN44323 ; SF2837 .
AAP18149 ; AAP18149 ; S3035 .
GeneIDi 1025814.
1079305.
KEGGi sfl:SF2837.
sfx:S3035.
PATRICi 18707758. VBIShiFle31049_3331.

Phylogenomic databases

eggNOGi COG0207.
HOGENOMi HOG000257899.
KOi K00560.
OMAi WRSWATA.
OrthoDBi EOG6K6V53.

Enzyme and pathway databases

UniPathwayi UPA00575 .

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 2 hits.
PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the wild-type and mutant thyA gene from Shigella flexneri Y."
    Nur-E-Kamal M.S., Al-Mamun A.A., Ahmed Z.U.
    Microbiol. Immunol. 38:309-312(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiTYSY_SHIFL
AccessioniPrimary (citable) accession number: P48464
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 10, 2003
Last modified: October 29, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3