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Protein

Serine/threonine-protein phosphatase beta isoform

Gene

flw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for cell adhesion in non-muscle tissues and in maintenance of muscle attachment. Vital for larval development.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Manganese 1By similarity1
Metal bindingi65Manganese 1By similarity1
Metal bindingi91Manganese 1By similarity1
Metal bindingi91Manganese 2By similarity1
Metal bindingi123Manganese 2By similarity1
Active sitei124Proton donorBy similarity1
Metal bindingi172Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • beta-catenin destruction complex disassembly Source: FlyBase
  • cell adhesion Source: UniProtKB-KW
  • chromosome segregation Source: FlyBase
  • circadian rhythm Source: Reactome
  • female germline ring canal formation Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • mesoderm development Source: FlyBase
  • negative regulation of JNK cascade Source: FlyBase
  • oocyte nucleus migration involved in oocyte dorsal/ventral axis specification Source: FlyBase
  • ovarian nurse cell to oocyte transport Source: FlyBase
  • positive regulation of canonical Wnt signaling pathway Source: FlyBase
  • protein dephosphorylation Source: FlyBase
  • regulation of actomyosin contractile ring contraction Source: FlyBase
  • striated muscle tissue development Source: FlyBase

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell adhesion
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-350384 Myosin phosphatase dephosphoylates SQH
R-DME-350480 Activation of non-muscle Myosin II
R-DME-538898 Dephosphorylation of TIM
SignaLinkiP48462

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase beta isoform (EC:3.1.3.161 Publication)
Alternative name(s):
Protein flap wing
Gene namesi
Name:flw
Synonyms:PP1-9C, PP1-BETA-9C
ORF Names:CG2096
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000711 flw

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133Y → F: Semi lethal, adult escapers exhibit muscle and wing mutant phenotype; allele flw-6. 1 Publication1
Mutagenesisi284V → A: Muscle and wing mutant phenotype; allele flw-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000587941 – 330Serine/threonine-protein phosphatase beta isoformAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei315Phosphothreonine1 Publication1
Modified residuei316Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48462
PRIDEiP48462

PTM databases

iPTMnetiP48462

Expressioni

Gene expression databases

BgeeiFBgn0000711
ExpressionAtlasiP48462 baseline and differential
GenevisibleiP48462 DM

Interactioni

Subunit structurei

Interacts with Nop17l (PubMed:17007873). Interacts with uri; uri inhibits flw phosphatase activity (PubMed:18412953).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nop17lQ0E9G33EBI-869621,EBI-150380

Protein-protein interaction databases

BioGridi68952, 129 interactors
IntActiP48462, 28 interactors
STRINGi7227.FBpp0071381

Structurei

3D structure databases

ProteinModelPortaliP48462
SMRiP48462
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN85 Eukaryota
ENOG410XPVF LUCA
GeneTreeiENSGT00530000062911
HOGENOMiHOG000263972
InParanoidiP48462
KOiK06269
OMAiDHQEADI
OrthoDBiEOG091G0EKF
PhylomeDBiP48462

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR037981 PPP1CC
IPR006186 Ser/Thr-sp_prot-phosphatase
IPR031675 STPPase_N
PANTHERiPTHR11668:SF204 PTHR11668:SF204, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF16891 STPPase_N, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

P48462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDFDLNVDS LIQRLLEMRS CRTGKQVQMT EAEVRGLCLK SREIFLQQPI
60 70 80 90 100
LLELEAPLII CGDIHGQYTD LLRLFEYGGF PPAANYLFLG DYVDRGKQSL
110 120 130 140 150
ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRYNVKLWKT
160 170 180 190 200
FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ GMEQIRRLMR PTDVPDTGLL
210 220 230 240 250
CDLLWSDPDK DVQGWGENDR GVSFTFGVDV VSKFLNRHEL DLICRAHQVV
260 270 280 290 300
EDGYEFFARR QLVTLFSAPN YCGEFDNAGG MMTVDDTLMC SFQILKPSEK
310 320 330
KAKYLYSGMN SSRPTTPQRS APMLATNKKK
Length:330
Mass (Da):37,740
Last modified:February 1, 1996 - v1
Checksum:i9233DFC06EAE17AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56439 mRNA Translation: CAA39821.1
AJ249214, AJ249215 Genomic DNA Translation: CAB59732.1
AE014298 Genomic DNA Translation: AAF46583.2
PIRiS13828
RefSeqiNP_001259407.1, NM_001272478.1
NP_524738.1, NM_079999.3
UniGeneiDm.7222

Genome annotation databases

EnsemblMetazoaiFBtr0071447; FBpp0071382; FBgn0000711
FBtr0333305; FBpp0305497; FBgn0000711
GeneIDi44289
KEGGidme:Dmel_CG2096

Similar proteinsi

Entry informationi

Entry nameiPP1B_DROME
AccessioniPrimary (citable) accession number: P48462
Secondary accession number(s): Q9W2V5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health