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Protein

Serine/threonine-protein phosphatase beta isoform

Gene

flw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cell adhesion in non-muscle tissues and in maintenance of muscle attachment. Vital for larval development.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Manganese 1By similarity1
Metal bindingi65Manganese 1By similarity1
Metal bindingi91Manganese 1By similarity1
Metal bindingi91Manganese 2By similarity1
Metal bindingi123Manganese 2By similarity1
Active sitei124Proton donorBy similarity1
Metal bindingi172Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • myosin phosphatase activity Source: FlyBase
  • protein serine/threonine phosphatase activity Source: FlyBase

GO - Biological processi

  • beta-catenin destruction complex disassembly Source: FlyBase
  • cell adhesion Source: UniProtKB-KW
  • chromosome segregation Source: FlyBase
  • female germline ring canal formation Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • mesoderm development Source: FlyBase
  • negative regulation of JNK cascade Source: FlyBase
  • oocyte nucleus migration involved in oocyte dorsal/ventral axis specification Source: FlyBase
  • ovarian nurse cell to oocyte transport Source: FlyBase
  • positive regulation of canonical Wnt signaling pathway Source: FlyBase
  • protein dephosphorylation Source: FlyBase
  • regulation of actomyosin contractile ring contraction Source: FlyBase
  • striated muscle tissue development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

SignaLinkiP48462.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase beta isoform (EC:3.1.3.161 Publication)
Alternative name(s):
Protein flap wing
Gene namesi
Name:flw
Synonyms:PP1-9C, PP1-BETA-9C
ORF Names:CG2096
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000711. flw.

Subcellular locationi

GO - Cellular componenti

  • protein phosphatase type 1 complex Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133Y → F: Semi lethal, adult escapers exhibit muscle and wing mutant phenotype; allele flw-6. 1 Publication1
Mutagenesisi284V → A: Muscle and wing mutant phenotype; allele flw-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000587941 – 330Serine/threonine-protein phosphatase beta isoformAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei315Phosphothreonine1 Publication1
Modified residuei316Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48462.
PRIDEiP48462.

PTM databases

iPTMnetiP48462.

Expressioni

Gene expression databases

BgeeiFBgn0000711.
GenevisibleiP48462. DM.

Interactioni

Subunit structurei

Interacts with Nop17l (PubMed:17007873). Interacts with uri; uri inhibits flw phosphatase activity (PubMed:18412953).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nop17lQ0E9G33EBI-869621,EBI-150380

Protein-protein interaction databases

BioGridi68952. 92 interactors.
IntActiP48462. 28 interactors.
STRINGi7227.FBpp0071381.

Structurei

3D structure databases

ProteinModelPortaliP48462.
SMRiP48462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN85. Eukaryota.
ENOG410XPVF. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000263972.
InParanoidiP48462.
KOiK06269.
OrthoDBiEOG091G0EKF.
PhylomeDBiP48462.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDFDLNVDS LIQRLLEMRS CRTGKQVQMT EAEVRGLCLK SREIFLQQPI
60 70 80 90 100
LLELEAPLII CGDIHGQYTD LLRLFEYGGF PPAANYLFLG DYVDRGKQSL
110 120 130 140 150
ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRYNVKLWKT
160 170 180 190 200
FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ GMEQIRRLMR PTDVPDTGLL
210 220 230 240 250
CDLLWSDPDK DVQGWGENDR GVSFTFGVDV VSKFLNRHEL DLICRAHQVV
260 270 280 290 300
EDGYEFFARR QLVTLFSAPN YCGEFDNAGG MMTVDDTLMC SFQILKPSEK
310 320 330
KAKYLYSGMN SSRPTTPQRS APMLATNKKK
Length:330
Mass (Da):37,740
Last modified:February 1, 1996 - v1
Checksum:i9233DFC06EAE17AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56439 mRNA. Translation: CAA39821.1.
AJ249214, AJ249215 Genomic DNA. Translation: CAB59732.1.
AE014298 Genomic DNA. Translation: AAF46583.2.
PIRiS13828.
RefSeqiNP_001259407.1. NM_001272478.1.
NP_524738.1. NM_079999.3.
UniGeneiDm.7222.

Genome annotation databases

EnsemblMetazoaiFBtr0071447; FBpp0071382; FBgn0000711.
FBtr0333305; FBpp0305497; FBgn0000711.
GeneIDi44289.
KEGGidme:Dmel_CG2096.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56439 mRNA. Translation: CAA39821.1.
AJ249214, AJ249215 Genomic DNA. Translation: CAB59732.1.
AE014298 Genomic DNA. Translation: AAF46583.2.
PIRiS13828.
RefSeqiNP_001259407.1. NM_001272478.1.
NP_524738.1. NM_079999.3.
UniGeneiDm.7222.

3D structure databases

ProteinModelPortaliP48462.
SMRiP48462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68952. 92 interactors.
IntActiP48462. 28 interactors.
STRINGi7227.FBpp0071381.

PTM databases

iPTMnetiP48462.

Proteomic databases

PaxDbiP48462.
PRIDEiP48462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0071447; FBpp0071382; FBgn0000711.
FBtr0333305; FBpp0305497; FBgn0000711.
GeneIDi44289.
KEGGidme:Dmel_CG2096.

Organism-specific databases

CTDi44289.
FlyBaseiFBgn0000711. flw.

Phylogenomic databases

eggNOGiENOG410IN85. Eukaryota.
ENOG410XPVF. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000263972.
InParanoidiP48462.
KOiK06269.
OrthoDBiEOG091G0EKF.
PhylomeDBiP48462.

Enzyme and pathway databases

SignaLinkiP48462.

Miscellaneous databases

ChiTaRSiflw. fly.
GenomeRNAii44289.
PROiP48462.

Gene expression databases

BgeeiFBgn0000711.
GenevisibleiP48462. DM.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP1B_DROME
AccessioniPrimary (citable) accession number: P48462
Secondary accession number(s): Q9W2V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.