P48457 (PP2B_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit
EC=3.1.3.16

Alternative name(s):
Calmodulin-dependent calcineurin A subunit

Gene names

Name:	cnaA
ORF Names:	AN8820

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 Fe3+ ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2B subfamily.
Sequence caution	The sequence AAA57873.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence EAA60108.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Ligand	Calmodulin-binding Iron Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Inferred from electronic annotation. Source: EnsemblFungi cellular ion homeostasis Inferred from electronic annotation. Source: EnsemblFungi fungal-type cell wall organization Inferred from electronic annotation. Source: EnsemblFungi
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Serine/threonine-protein phosphatase 2B catalytic subunit		PRO_0000058834
Sites
	Active site	173	1	Proton donor By similarity
	Metal binding	112	1	Iron By similarity
	Metal binding	114	1	Iron By similarity
	Metal binding	140	1	Iron By similarity
	Metal binding	140	1	Zinc By similarity
	Metal binding	172	1	Zinc By similarity
	Metal binding	221	1	Zinc By similarity
	Metal binding	303	1	Zinc By similarity
Experimental info
	Sequence conflict	386 – 387	2	ML → IV in AAA57873. Ref.1
	Sequence conflict	541	1	M → MVRRIR in AAA57873. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48457 [UniParc]. Last modified November 16, 2011. Version 3. Checksum: ECD795FCBBDF4991	FASTA	549	63,007
	10 20 30 40 50 60 MDRNLARAVA DKQPVPEIDF TLHVMEDGTQ VSTLERVVKE VQAPALNKPS DDQFWDPEEP 70 80 90 100 110 120 TKPNLQFLKQ HFYREGRLTE DQALWIIQAG TQILKSEPNL LEMDAPITVC GDVHGQYYDL 130 140 150 160 170 180 MKLFEVGGDP AETRYLFLGD YVDRGYFSIE CVLYLWALKI WYPNTLWLLR GNHECRHLTD 190 200 210 220 230 240 YFTFKLECKH KYSERIYEAC IESFCALPLA AVMNKQFLCI HGGLSPELHT LEDIKSIDRF 250 260 270 280 290 300 REPPTHGLMC DILWADPLED FGQEKTGDYF IHNSVRGCSY FFSYPAACAF LEKNNLLSVI 310 320 330 340 350 360 RAHEAQDAGY RMYRKTRTTG FPSVMTIFSA PNYLDVYNNK AAVLKYENNV MNIRQFNCTP 370 380 390 400 410 420 HPYWLPNFMD VFTWSLPFVG EKITDMLIAI LNTCSKEELE DETPSTISPA EPSPPMPMDT 430 440 450 460 470 480 VDTESTEFKR RAIKNKILAI GRLSRVFQVL REESERVTEL KTAAGGRLPA GTLMLGAEGI 490 500 510 520 530 540 KQAITNFEDA RKVDLQNERL PPSHDEVVRR SEEERRIALD RAQHEADNDT GLATVARRIS MKIPSTTRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The calmodulin-dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans." Rasmussen C.D., Garen C., Brining S., Kincaid R.L., Means R.L., Means A.R. EMBO J. 13:2545-2552(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13919 Genomic DNA. Translation: AAA57873.1. Different initiation. AACD01000162 Genomic DNA. Translation: EAA60108.1. Different initiation. BN001303 Genomic DNA. Translation: CBF77949.1.
PIR	S46322.
RefSeq	XP_682089.1. XM_676997.1.
3D structure databases
ProteinModelPortal	P48457.
ModBase	Search...
Protein-protein interaction databases
STRING	162425.CADANIAP00006237.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2868443.
KEGG	ani:AN8820.2.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172699.
KO	K04348.
OMA	VIQECTS.
OrthoDB	EOG45XC4G.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2B_EMENI

Accession

Primary (citable) accession number: P48457
Secondary accession number(s): C8V9L3, Q5ASB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 16, 2011
Last modified:	April 3, 2013
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program