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P48457 (PP2B_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit

EC=3.1.3.16
Alternative name(s):
Calmodulin-dependent calcineurin A subunit
Gene names
Name:cnaA
ORF Names:AN8820
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 Fe3+ ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Sequence caution

The sequence AAA57873.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence EAA60108.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Serine/threonine-protein phosphatase 2B catalytic subunit
PRO_0000058834

Sites

Active site1731Proton donor By similarity
Metal binding1121Iron By similarity
Metal binding1141Iron By similarity
Metal binding1401Iron By similarity
Metal binding1401Zinc By similarity
Metal binding1721Zinc By similarity
Metal binding2211Zinc By similarity
Metal binding3031Zinc By similarity

Experimental info

Sequence conflict386 – 3872ML → IV in AAA57873. Ref.1
Sequence conflict5411M → MVRRIR in AAA57873. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48457 [UniParc].

Last modified November 16, 2011. Version 3.
Checksum: ECD795FCBBDF4991

FASTA54963,007
        10         20         30         40         50         60 
MDRNLARAVA DKQPVPEIDF TLHVMEDGTQ VSTLERVVKE VQAPALNKPS DDQFWDPEEP 

        70         80         90        100        110        120 
TKPNLQFLKQ HFYREGRLTE DQALWIIQAG TQILKSEPNL LEMDAPITVC GDVHGQYYDL 

       130        140        150        160        170        180 
MKLFEVGGDP AETRYLFLGD YVDRGYFSIE CVLYLWALKI WYPNTLWLLR GNHECRHLTD 

       190        200        210        220        230        240 
YFTFKLECKH KYSERIYEAC IESFCALPLA AVMNKQFLCI HGGLSPELHT LEDIKSIDRF 

       250        260        270        280        290        300 
REPPTHGLMC DILWADPLED FGQEKTGDYF IHNSVRGCSY FFSYPAACAF LEKNNLLSVI 

       310        320        330        340        350        360 
RAHEAQDAGY RMYRKTRTTG FPSVMTIFSA PNYLDVYNNK AAVLKYENNV MNIRQFNCTP 

       370        380        390        400        410        420 
HPYWLPNFMD VFTWSLPFVG EKITDMLIAI LNTCSKEELE DETPSTISPA EPSPPMPMDT 

       430        440        450        460        470        480 
VDTESTEFKR RAIKNKILAI GRLSRVFQVL REESERVTEL KTAAGGRLPA GTLMLGAEGI 

       490        500        510        520        530        540 
KQAITNFEDA RKVDLQNERL PPSHDEVVRR SEEERRIALD RAQHEADNDT GLATVARRIS 


MKIPSTTRR 

« Hide

References

« Hide 'large scale' references
[1]"The calmodulin-dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans."
Rasmussen C.D., Garen C., Brining S., Kincaid R.L., Means R.L., Means A.R.
EMBO J. 13:2545-2552(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U13919 Genomic DNA. Translation: AAA57873.1. Different initiation.
AACD01000162 Genomic DNA. Translation: EAA60108.1. Different initiation.
BN001303 Genomic DNA. Translation: CBF77949.1.
PIRS46322.
RefSeqXP_682089.1. XM_676997.1.

3D structure databases

ProteinModelPortalP48457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING162425.CADANIAP00006237.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00006237; CADANIAP00006237; CADANIAG00006237.
GeneID2868443.
KEGGani:AN8820.2.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172699.
KOK04348.
OMARTKPNIQ.
OrthoDBEOG77M8X9.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2B_EMENI
AccessionPrimary (citable) accession number: P48457
Secondary accession number(s): C8V9L3, Q5ASB0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 16, 2011
Last modified: April 16, 2014
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families