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P48456

- PP2B1_DROME

UniProt

P48456 - PP2B1_DROME

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit 1

Gene

CanA1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 Fe3+ ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi130 – 1301IronBy similarity
    Metal bindingi132 – 1321IronBy similarity
    Metal bindingi158 – 1581IronBy similarity
    Metal bindingi158 – 1581ZincBy similarity
    Metal bindingi190 – 1901ZincBy similarity
    Active sitei191 – 1911Proton donorBy similarity
    Metal bindingi239 – 2391ZincBy similarity
    Metal bindingi321 – 3211ZincBy similarity

    GO - Molecular functioni

    1. calcium-dependent protein serine/threonine phosphatase activity Source: FlyBase
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. medium-term memory Source: FlyBase
    2. neurotransmitter secretion Source: FlyBase
    3. protein dephosphorylation Source: FlyBase
    4. sleep Source: FlyBase
    5. vesicle-mediated transport Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Calmodulin-binding, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_184321. Ca2+ pathway.
    REACT_203584. FCERI mediated Ca+2 mobilization.
    REACT_213958. DARPP-32 events.
    SignaLinkiP48456.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit 1 (EC:3.1.3.16)
    Alternative name(s):
    Calmodulin-dependent calcineurin A1 subunit
    Gene namesi
    Name:CanA1
    Synonyms:CNA1, PpD14
    ORF Names:CG1455
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0010015. CanA1.

    Subcellular locationi

    GO - Cellular componenti

    1. calcineurin complex Source: FlyBase
    2. synaptic vesicle Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Serine/threonine-protein phosphatase 2B catalytic subunit 1PRO_0000058830Add
    BLAST

    Proteomic databases

    PaxDbiP48456.
    PRIDEiP48456.

    Expressioni

    Gene expression databases

    BgeeiP48456.

    Interactioni

    Subunit structurei

    Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

    Protein-protein interaction databases

    BioGridi68520. 14 interactions.
    IntActiP48456. 4 interactions.
    MINTiMINT-339738.

    Structurei

    3D structure databases

    ProteinModelPortaliP48456.
    SMRiP48456. Positions 1-412.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063087.
    InParanoidiP48456.
    KOiK04348.
    OMAiSACCEFL.
    OrthoDBiEOG7BZVSC.
    PhylomeDBiP48456.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 2 hits.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48456-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSATSTRSSV TRKSSLSKSS SSDKSAKSSS NSSKSPTAAS GNKQKMQYTK    50
    TRERMVDDVP LPPTHKLTMS EVYDDPKTGK PNFDALRQHF LLEGRIEEAV 100
    ALRIITEGAA LLREEKNMID VEAPITVCGD IHGQFFDLVK LFEVGGPPAT 150
    TRYLFLGDYV DRGYFSIECV LYLWSLKITY PTTLSLLRGN HECRHLTEYF 200
    TFKQECIIKY SESIYDACME AFDCLPLAAL LNQQFLCIHG GLSPEIFTLD 250
    DIKTLNRFRE PPAYGPMCDL LWSDPLEDFG NEKTNEFFSH NSVRGCSYFF 300
    SYSACCEFLQ KNNLLSIVRA HEAQDAGYRM YRKNQVTGFP SLITIFSAPN 350
    YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF TWSLPFVGEK 400
    VTEMLVNILN ICSDDELVAG PDDELEEELR KKIVLVPANA SNNNNNNNTP 450
    SKPASMSALR KEIIRNKIRA IGKMSRVFSI LREESESVLQ LKGLTPTGAL 500
    PVGALSGGRD SLKEALQGLT ASSHIHSFAE AKGLDAVNER MPPRRPLLMS 550
    ASSSSITTVT RSSSSSSNNN NNNSNTSSTT TTKDISNTSS NDTATVTKTS 600
    RTTVKSATTS NVRAGFTAKK FP 622
    Length:622
    Mass (Da):69,133
    Last modified:August 30, 2005 - v2
    Checksum:iFAD967D2123EFAE9
    GO

    Sequence cautioni

    The sequence AAA28410.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti459 – 4591L → V in AAA28410. (PubMed:1331060)Curated
    Sequence conflicti622 – 6221P → S in AAA28410. (PubMed:1331060)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF57105.3.
    AY128480 mRNA. Translation: AAM75073.1.
    M97012 mRNA. Translation: AAA28410.1. Different initiation.
    S39996 Genomic DNA. Translation: AAB22466.1.
    PIRiB44307.
    RefSeqiNP_001247378.2. NM_001260449.2.
    NP_524600.3. NM_079861.4.
    UniGeneiDm.1902.

    Genome annotation databases

    EnsemblMetazoaiFBtr0085732; FBpp0085094; FBgn0010015.
    GeneIDi43670.
    KEGGidme:Dmel_CG1455.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF57105.3 .
    AY128480 mRNA. Translation: AAM75073.1 .
    M97012 mRNA. Translation: AAA28410.1 . Different initiation.
    S39996 Genomic DNA. Translation: AAB22466.1 .
    PIRi B44307.
    RefSeqi NP_001247378.2. NM_001260449.2.
    NP_524600.3. NM_079861.4.
    UniGenei Dm.1902.

    3D structure databases

    ProteinModelPortali P48456.
    SMRi P48456. Positions 1-412.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 68520. 14 interactions.
    IntActi P48456. 4 interactions.
    MINTi MINT-339738.

    Proteomic databases

    PaxDbi P48456.
    PRIDEi P48456.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0085732 ; FBpp0085094 ; FBgn0010015 .
    GeneIDi 43670.
    KEGGi dme:Dmel_CG1455.

    Organism-specific databases

    CTDi 43670.
    FlyBasei FBgn0010015. CanA1.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063087.
    InParanoidi P48456.
    KOi K04348.
    OMAi SACCEFL.
    OrthoDBi EOG7BZVSC.
    PhylomeDBi P48456.

    Enzyme and pathway databases

    Reactomei REACT_184321. Ca2+ pathway.
    REACT_203584. FCERI mediated Ca+2 mobilization.
    REACT_213958. DARPP-32 events.
    SignaLinki P48456.

    Miscellaneous databases

    GenomeRNAii 43670.
    NextBioi 835180.

    Gene expression databases

    Bgeei P48456.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 2 hits.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    4. "Molecular cloning and characterization of the genes encoding the two subunits of Drosophila melanogaster calcineurin."
      Guerini D., Montell C., Klee C.B.
      J. Biol. Chem. 267:22542-22549(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-622.
    5. "Polymerase chain reactions using Saccharomyces, Drosophila and human DNA predict a large family of protein serine/threonine phosphatases."
      Chen M.X., Chen Y.H., Cohen P.T.W.
      FEBS Lett. 306:54-58(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 134-157.

    Entry informationi

    Entry nameiPP2B1_DROME
    AccessioniPrimary (citable) accession number: P48456
    Secondary accession number(s): Q8MQN3, Q9TY69, Q9VA20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3