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P48454 (PP2BC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform

EC=3.1.3.16
Alternative name(s):
CAM-PRP catalytic subunit
Calcineurin, testis-specific catalytic subunit
Calmodulin-dependent calcineurin A subunit gamma isoform
Gene names
Name:PPP3CC
Synonyms:CALNA3, CNA3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 Fe3+ ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Tissue specificity

Testis.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P48454-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P48454-2)

The sequence of this isoform differs from the canonical sequence as follows:
     442-451: Missing.
Isoform 3 (identifier: P48454-3)

The sequence of this isoform differs from the canonical sequence as follows:
     380-380: E → EDHYIPSYQK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform
PRO_0000058828

Sites

Active site1471Proton donor By similarity
Metal binding861Iron By similarity
Metal binding881Iron By similarity
Metal binding1141Iron By similarity
Metal binding1141Zinc By similarity
Metal binding1461Zinc By similarity
Metal binding1951Zinc By similarity
Metal binding2771Zinc By similarity

Natural variations

Alternative sequence3801E → EDHYIPSYQK in isoform 3.
VSP_045211
Alternative sequence442 – 45110Missing in isoform 2.
VSP_037946
Natural variant5011A → V.
Corresponds to variant rs28764007 [ dbSNP | Ensembl ].
VAR_061758

Experimental info

Sequence conflict485 – 4862HA → YP in AAB23769. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 1, 2009. Version 3.
Checksum: 1C3AA7996660D575

FASTA51258,129
        10         20         30         40         50         60 
MSGRRFHLST TDRVIKAVPF PPTQRLTFKE VFENGKPKVD VLKNHLVKEG RLEEEVALKI 

        70         80         90        100        110        120 
INDGAAILRQ EKTMIEVDAP ITVCGDIHGQ FFDLMKLFEV GGSPSNTRYL FLGDYVDRGY 

       130        140        150        160        170        180 
FSIECVLYLW SLKINHPKTL FLLRGNHECR HLTDYFTFKQ ECRIKYSEQV YDACMETFDC 

       190        200        210        220        230        240 
LPLAALLNQQ FLCVHGGMSP EITSLDDIRK LDRFTEPPAF GPVCDLLWSD PSEDYGNEKT 

       250        260        270        280        290        300 
LEHYTHNTVR GCSYFYSYPA VCEFLQNNNL LSIIRAHEAQ DAGYRMYRKS QATGFPSLIT 

       310        320        330        340        350        360 
IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM 

       370        380        390        400        410        420 
LVNVLNICSD DELISDDEAE GSTTVRKEII RNKIRAIGKM ARVFSILRQE SESVLTLKGL 

       430        440        450        460        470        480 
TPTGTLPLGV LSGGKQTIET ATVEAVEARE AIRGFSLQHK IRSFEEARGL DRINERMPPR 

       490        500        510 
KDSIHAGGPM KSVTSAHSHA AHRSDQGKKA HS 

« Hide

Isoform 2 [UniParc].

Checksum: 8ED89CC321A6DF0B
Show »

FASTA50257,073
Isoform 3 [UniParc].

Checksum: BC240A119202E701
Show »

FASTA52159,262

References

« Hide 'large scale' references
[1]"Molecular cloning and chromosomal mapping of the human gene for the testis-specific catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A)."
Muramatsu T., Kincaid R.L.
Biochem. Biophys. Res. Commun. 188:265-271(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Testis.
[2]"Isoform specific expression of calcineurin in endothelial cells."
Bako E., Aydonian A., Verin A.D., Garcia J.G.N.
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Endothelial cell.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Tongue.
[4]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymphoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S46622 mRNA. Translation: AAB23769.1.
AY007249 mRNA. Translation: AAG02563.1.
AK299415 mRNA. Translation: BAG61397.1.
AC037459 Genomic DNA. No translation available.
AC087854 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63677.1.
CH471080 Genomic DNA. Translation: EAW63679.1.
BC004864 mRNA. Translation: AAH04864.1.
CCDSCCDS34859.1. [P48454-1]
CCDS59093.1. [P48454-3]
CCDS59094.1. [P48454-2]
PIRJC1283.
RefSeqNP_001230903.1. NM_001243974.1. [P48454-3]
NP_001230904.1. NM_001243975.1. [P48454-2]
NP_005596.2. NM_005605.4. [P48454-1]
UniGeneHs.731683.

3D structure databases

ProteinModelPortalP48454.
SMRP48454. Positions 8-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111525. 10 interactions.
IntActP48454. 7 interactions.
STRING9606.ENSP00000240139.

Chemistry

BindingDBP48454.

PTM databases

PhosphoSiteP48454.

Polymorphism databases

DMDM257051041.

Proteomic databases

MaxQBP48454.
PaxDbP48454.
PRIDEP48454.

Protocols and materials databases

DNASU5533.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000240139; ENSP00000240139; ENSG00000120910. [P48454-1]
ENST00000289963; ENSP00000289963; ENSG00000120910. [P48454-2]
ENST00000397775; ENSP00000380878; ENSG00000120910. [P48454-3]
GeneID5533.
KEGGhsa:5533.
UCSCuc003xbs.3. human. [P48454-1]
uc003xbt.3. human. [P48454-2]
uc011kzi.2. human.

Organism-specific databases

CTD5533.
GeneCardsGC08P022298.
H-InvDBHIX0201288.
HGNCHGNC:9316. PPP3CC.
HPACAB024950.
HPA023396.
MIM114107. gene.
neXtProtNX_P48454.
PharmGKBPA33680.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172699.
HOVERGENHBG002819.
KOK04348.
OMAKINYPKT.
PhylomeDBP48454.
TreeFamTF105557.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_578. Apoptosis.

Gene expression databases

ArrayExpressP48454.
BgeeP48454.
CleanExHS_PPP3CC.
GenevestigatorP48454.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPPP3CC. human.
GeneWikiPPP3CC.
GenomeRNAi5533.
NextBio21434.
PROP48454.
SOURCESearch...

Entry information

Entry namePP2BC_HUMAN
AccessionPrimary (citable) accession number: P48454
Secondary accession number(s): B4DRT5, Q9BSS6, Q9H4M5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 1, 2009
Last modified: July 9, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM