P48454 (PP2BC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform
EC=3.1.3.16

Alternative name(s):
CAM-PRP catalytic subunit
Calcineurin, testis-specific catalytic subunit
Calmodulin-dependent calcineurin A subunit gamma isoform

Gene names

Name:	PPP3CC
Synonyms:	CALNA3, CNA3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 Fe3+ ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.
Tissue specificity	Testis.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2B subfamily.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Calmodulin-binding Iron Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P48454-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P48454-2)

The sequence of this isoform differs from the canonical sequence as follows:
     442-451: Missing.

Isoform 3 (identifier: P48454-3)

The sequence of this isoform differs from the canonical sequence as follows:
     380-380: E → EDHYIPSYQK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform		PRO_0000058828
Sites
	Active site	147	1	Proton donor By similarity
	Metal binding	86	1	Iron By similarity
	Metal binding	88	1	Iron By similarity
	Metal binding	114	1	Iron By similarity
	Metal binding	114	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	195	1	Zinc By similarity
	Metal binding	277	1	Zinc By similarity
Natural variations
	Alternative sequence	380	1	E → EDHYIPSYQK in isoform 3.		VSP_045211
	Alternative sequence	442 – 451	10	Missing in isoform 2.		VSP_037946
	Natural variant	501	1	A → V. Corresponds to variant rs28764007 [ dbSNP | Ensembl ].		VAR_061758
Experimental info
	Sequence conflict	485 – 486	2	HA → YP in AAB23769. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 3. Checksum: 1C3AA7996660D575	FASTA	512	58,129
	10 20 30 40 50 60 MSGRRFHLST TDRVIKAVPF PPTQRLTFKE VFENGKPKVD VLKNHLVKEG RLEEEVALKI 70 80 90 100 110 120 INDGAAILRQ EKTMIEVDAP ITVCGDIHGQ FFDLMKLFEV GGSPSNTRYL FLGDYVDRGY 130 140 150 160 170 180 FSIECVLYLW SLKINHPKTL FLLRGNHECR HLTDYFTFKQ ECRIKYSEQV YDACMETFDC 190 200 210 220 230 240 LPLAALLNQQ FLCVHGGMSP EITSLDDIRK LDRFTEPPAF GPVCDLLWSD PSEDYGNEKT 250 260 270 280 290 300 LEHYTHNTVR GCSYFYSYPA VCEFLQNNNL LSIIRAHEAQ DAGYRMYRKS QATGFPSLIT 310 320 330 340 350 360 IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM 370 380 390 400 410 420 LVNVLNICSD DELISDDEAE GSTTVRKEII RNKIRAIGKM ARVFSILRQE SESVLTLKGL 430 440 450 460 470 480 TPTGTLPLGV LSGGKQTIET ATVEAVEARE AIRGFSLQHK IRSFEEARGL DRINERMPPR 490 500 510 KDSIHAGGPM KSVTSAHSHA AHRSDQGKKA HS
	Isoform 2 [UniParc]. Checksum: 8ED89CC321A6DF0B	FASTA	502	57,073
	10 20 30 40 50 60 MSGRRFHLST TDRVIKAVPF PPTQRLTFKE VFENGKPKVD VLKNHLVKEG RLEEEVALKI 70 80 90 100 110 120 INDGAAILRQ EKTMIEVDAP ITVCGDIHGQ FFDLMKLFEV GGSPSNTRYL FLGDYVDRGY 130 140 150 160 170 180 FSIECVLYLW SLKINHPKTL FLLRGNHECR HLTDYFTFKQ ECRIKYSEQV YDACMETFDC 190 200 210 220 230 240 LPLAALLNQQ FLCVHGGMSP EITSLDDIRK LDRFTEPPAF GPVCDLLWSD PSEDYGNEKT 250 260 270 280 290 300 LEHYTHNTVR GCSYFYSYPA VCEFLQNNNL LSIIRAHEAQ DAGYRMYRKS QATGFPSLIT 310 320 330 340 350 360 IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM 370 380 390 400 410 420 LVNVLNICSD DELISDDEAE GSTTVRKEII RNKIRAIGKM ARVFSILRQE SESVLTLKGL 430 440 450 460 470 480 TPTGTLPLGV LSGGKQTIET AIRGFSLQHK IRSFEEARGL DRINERMPPR KDSIHAGGPM 490 500 KSVTSAHSHA AHRSDQGKKA HS
	Isoform 3 [UniParc]. Checksum: BC240A119202E701	FASTA	521	59,262
	10 20 30 40 50 60 MSGRRFHLST TDRVIKAVPF PPTQRLTFKE VFENGKPKVD VLKNHLVKEG RLEEEVALKI 70 80 90 100 110 120 INDGAAILRQ EKTMIEVDAP ITVCGDIHGQ FFDLMKLFEV GGSPSNTRYL FLGDYVDRGY 130 140 150 160 170 180 FSIECVLYLW SLKINHPKTL FLLRGNHECR HLTDYFTFKQ ECRIKYSEQV YDACMETFDC 190 200 210 220 230 240 LPLAALLNQQ FLCVHGGMSP EITSLDDIRK LDRFTEPPAF GPVCDLLWSD PSEDYGNEKT 250 260 270 280 290 300 LEHYTHNTVR GCSYFYSYPA VCEFLQNNNL LSIIRAHEAQ DAGYRMYRKS QATGFPSLIT 310 320 330 340 350 360 IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM 370 380 390 400 410 420 LVNVLNICSD DELISDDEAE DHYIPSYQKG STTVRKEIIR NKIRAIGKMA RVFSILRQES 430 440 450 460 470 480 ESVLTLKGLT PTGTLPLGVL SGGKQTIETA TVEAVEAREA IRGFSLQHKI RSFEEARGLD 490 500 510 520 RINERMPPRK DSIHAGGPMK SVTSAHSHAA HRSDQGKKAH S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and chromosomal mapping of the human gene for the testis-specific catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A)." Muramatsu T., Kincaid R.L. Biochem. Biophys. Res. Commun. 188:265-271(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"Isoform specific expression of calcineurin in endothelial cells." Bako E., Aydonian A., Verin A.D., Garcia J.G.N. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Endothelial cell.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Tongue.
[4]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymphoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S46622 mRNA. Translation: AAB23769.1. AY007249 mRNA. Translation: AAG02563.1. AK299415 mRNA. Translation: BAG61397.1. AC037459 Genomic DNA. No translation available. AC087854 Genomic DNA. No translation available. CH471080 Genomic DNA. Translation: EAW63677.1. CH471080 Genomic DNA. Translation: EAW63679.1. BC004864 mRNA. Translation: AAH04864.1.
IPI	IPI00305491. IPI00413731.
PIR	JC1283.
RefSeq	NP_001230903.1. NM_001243974.1. NP_001230904.1. NM_001243975.1. NP_005596.2. NM_005605.4.
UniGene	Hs.731683.
3D structure databases
ProteinModelPortal	P48454.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48454. 7 interactions.
STRING	9606.ENSP00000240139.
PTM databases
PhosphoSite	P48454.
Polymorphism databases
DMDM	257051041.
Proteomic databases
PaxDb	P48454.
PRIDE	P48454.
Protocols and materials databases
DNASU	5533.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000240139; ENSP00000240139; ENSG00000120910. ENST00000289963; ENSP00000289963; ENSG00000120910. ENST00000397775; ENSP00000380878; ENSG00000120910.
GeneID	5533.
KEGG	hsa:5533.
UCSC	uc003xbr.2. human. uc003xbt.3. human.
Organism-specific databases
CTD	5533.
GeneCards	GC08P022298.
H-InvDB	HIX0201288.
HGNC	HGNC:9316. PPP3CC.
HPA	CAB024950. HPA023396.
MIM	114107. gene.
neXtProt	NX_P48454.
PharmGKB	PA33680.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172699.
HOVERGEN	HBG002819.
KO	K04348.
OMA	DEAEDHY.
OrthoDB	EOG437RDR.
Enzyme and pathway databases
Pathway_Interaction_DB	bcr_5pathway. BCR signaling pathway.
Reactome	REACT_111102. Signal Transduction. REACT_578. Apoptosis.
Gene expression databases
ArrayExpress	P48454.
Bgee	P48454.
CleanEx	HS_PPP3CC.
Genevestigator	P48454.
GermOnline	ENSG00000120910. Homo sapiens.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P48454.
ChEMBL	CHEMBL2694.
ChiTaRS	PPP3CC. human.
GenomeRNAi	5533.
NextBio	21434.
SOURCE	Search...

Entry information

Entry name

PP2BC_HUMAN

Accession

Primary (citable) accession number: P48454
Secondary accession number(s): B4DRT5, Q9BSS6, Q9H4M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.