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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform

Gene

PPP3CC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861IronBy similarity
Metal bindingi88 – 881IronBy similarity
Metal bindingi114 – 1141IronBy similarity
Metal bindingi114 – 1141ZincBy similarity
Metal bindingi146 – 1461ZincBy similarity
Active sitei147 – 1471Proton donorBy similarity
Metal bindingi195 – 1951ZincBy similarity
Metal bindingi277 – 2771ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_15334. DARPP-32 events.
REACT_549. Activation of BAD and translocation to mitochondria.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (EC:3.1.3.16)
Alternative name(s):
CAM-PRP catalytic subunit
Calcineurin, testis-specific catalytic subunit
Calmodulin-dependent calcineurin A subunit gamma isoform
Gene namesi
Name:PPP3CC
Synonyms:CALNA3, CNA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:9316. PPP3CC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33680.

Polymorphism and mutation databases

BioMutaiPPP3CC.
DMDMi257051041.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoformPRO_0000058828Add
BLAST

Proteomic databases

MaxQBiP48454.
PaxDbiP48454.
PRIDEiP48454.

PTM databases

DEPODiP48454.
PhosphoSiteiP48454.

Expressioni

Tissue specificityi

Testis.

Gene expression databases

BgeeiP48454.
CleanExiHS_PPP3CC.
ExpressionAtlasiP48454. baseline and differential.
GenevisibleiP48454. HS.

Organism-specific databases

HPAiCAB024950.
HPA023396.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
AMPHP494183EBI-2827192,EBI-7121510

Protein-protein interaction databases

BioGridi111525. 27 interactions.
IntActiP48454. 8 interactions.
STRINGi9606.ENSP00000240139.

Structurei

3D structure databases

ProteinModelPortaliP48454.
SMRiP48454. Positions 8-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
HOVERGENiHBG002819.
InParanoidiP48454.
KOiK04348.
OMAiXTVEAVE.
PhylomeDBiP48454.
TreeFamiTF105557.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48454-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGRRFHLST TDRVIKAVPF PPTQRLTFKE VFENGKPKVD VLKNHLVKEG
60 70 80 90 100
RLEEEVALKI INDGAAILRQ EKTMIEVDAP ITVCGDIHGQ FFDLMKLFEV
110 120 130 140 150
GGSPSNTRYL FLGDYVDRGY FSIECVLYLW SLKINHPKTL FLLRGNHECR
160 170 180 190 200
HLTDYFTFKQ ECRIKYSEQV YDACMETFDC LPLAALLNQQ FLCVHGGMSP
210 220 230 240 250
EITSLDDIRK LDRFTEPPAF GPVCDLLWSD PSEDYGNEKT LEHYTHNTVR
260 270 280 290 300
GCSYFYSYPA VCEFLQNNNL LSIIRAHEAQ DAGYRMYRKS QATGFPSLIT
310 320 330 340 350
IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL
360 370 380 390 400
PFVGEKVTEM LVNVLNICSD DELISDDEAE GSTTVRKEII RNKIRAIGKM
410 420 430 440 450
ARVFSILRQE SESVLTLKGL TPTGTLPLGV LSGGKQTIET ATVEAVEARE
460 470 480 490 500
AIRGFSLQHK IRSFEEARGL DRINERMPPR KDSIHAGGPM KSVTSAHSHA
510
AHRSDQGKKA HS
Length:512
Mass (Da):58,129
Last modified:September 1, 2009 - v3
Checksum:i1C3AA7996660D575
GO
Isoform 2 (identifier: P48454-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     442-451: Missing.

Show »
Length:502
Mass (Da):57,073
Checksum:i8ED89CC321A6DF0B
GO
Isoform 3 (identifier: P48454-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     380-380: E → EDHYIPSYQK

Show »
Length:521
Mass (Da):59,262
Checksum:iBC240A119202E701
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti485 – 4862HA → YP in AAB23769 (PubMed:1339277).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti501 – 5011A → V.
Corresponds to variant rs28764007 [ dbSNP | Ensembl ].
VAR_061758

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei380 – 3801E → EDHYIPSYQK in isoform 3. 1 PublicationVSP_045211
Alternative sequencei442 – 45110Missing in isoform 2. 2 PublicationsVSP_037946

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46622 mRNA. Translation: AAB23769.1.
AY007249 mRNA. Translation: AAG02563.1.
AK299415 mRNA. Translation: BAG61397.1.
AC037459 Genomic DNA. No translation available.
AC087854 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63677.1.
CH471080 Genomic DNA. Translation: EAW63679.1.
BC004864 mRNA. Translation: AAH04864.1.
CCDSiCCDS34859.1. [P48454-1]
CCDS59093.1. [P48454-3]
CCDS59094.1. [P48454-2]
PIRiJC1283.
RefSeqiNP_001230903.1. NM_001243974.1. [P48454-3]
NP_001230904.1. NM_001243975.1. [P48454-2]
NP_005596.2. NM_005605.4. [P48454-1]
UniGeneiHs.731683.

Genome annotation databases

EnsembliENST00000240139; ENSP00000240139; ENSG00000120910. [P48454-1]
ENST00000289963; ENSP00000289963; ENSG00000120910. [P48454-2]
ENST00000397775; ENSP00000380878; ENSG00000120910. [P48454-3]
GeneIDi5533.
KEGGihsa:5533.
UCSCiuc003xbs.3. human. [P48454-1]
uc003xbt.3. human. [P48454-2]
uc011kzi.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46622 mRNA. Translation: AAB23769.1.
AY007249 mRNA. Translation: AAG02563.1.
AK299415 mRNA. Translation: BAG61397.1.
AC037459 Genomic DNA. No translation available.
AC087854 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63677.1.
CH471080 Genomic DNA. Translation: EAW63679.1.
BC004864 mRNA. Translation: AAH04864.1.
CCDSiCCDS34859.1. [P48454-1]
CCDS59093.1. [P48454-3]
CCDS59094.1. [P48454-2]
PIRiJC1283.
RefSeqiNP_001230903.1. NM_001243974.1. [P48454-3]
NP_001230904.1. NM_001243975.1. [P48454-2]
NP_005596.2. NM_005605.4. [P48454-1]
UniGeneiHs.731683.

3D structure databases

ProteinModelPortaliP48454.
SMRiP48454. Positions 8-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111525. 27 interactions.
IntActiP48454. 8 interactions.
STRINGi9606.ENSP00000240139.

PTM databases

DEPODiP48454.
PhosphoSiteiP48454.

Polymorphism and mutation databases

BioMutaiPPP3CC.
DMDMi257051041.

Proteomic databases

MaxQBiP48454.
PaxDbiP48454.
PRIDEiP48454.

Protocols and materials databases

DNASUi5533.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000240139; ENSP00000240139; ENSG00000120910. [P48454-1]
ENST00000289963; ENSP00000289963; ENSG00000120910. [P48454-2]
ENST00000397775; ENSP00000380878; ENSG00000120910. [P48454-3]
GeneIDi5533.
KEGGihsa:5533.
UCSCiuc003xbs.3. human. [P48454-1]
uc003xbt.3. human. [P48454-2]
uc011kzi.2. human.

Organism-specific databases

CTDi5533.
GeneCardsiGC08P022298.
H-InvDBHIX0201288.
HGNCiHGNC:9316. PPP3CC.
HPAiCAB024950.
HPA023396.
MIMi114107. gene.
neXtProtiNX_P48454.
PharmGKBiPA33680.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
HOVERGENiHBG002819.
InParanoidiP48454.
KOiK04348.
OMAiXTVEAVE.
PhylomeDBiP48454.
TreeFamiTF105557.

Enzyme and pathway databases

ReactomeiREACT_15334. DARPP-32 events.
REACT_549. Activation of BAD and translocation to mitochondria.

Miscellaneous databases

ChiTaRSiPPP3CC. human.
GeneWikiiPPP3CC.
GenomeRNAii5533.
NextBioi21434.
PROiP48454.
SOURCEiSearch...

Gene expression databases

BgeeiP48454.
CleanExiHS_PPP3CC.
ExpressionAtlasiP48454. baseline and differential.
GenevisibleiP48454. HS.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and chromosomal mapping of the human gene for the testis-specific catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A)."
    Muramatsu T., Kincaid R.L.
    Biochem. Biophys. Res. Commun. 188:265-271(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Testis.
  2. "Isoform specific expression of calcineurin in endothelial cells."
    Bako E., Aydonian A., Verin A.D., Garcia J.G.N.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Endothelial cell.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Tongue.
  4. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymphoma.

Entry informationi

Entry nameiPP2BC_HUMAN
AccessioniPrimary (citable) accession number: P48454
Secondary accession number(s): B4DRT5, Q9BSS6, Q9H4M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 1, 2009
Last modified: June 24, 2015
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.