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Reviewed, UniProtKB/Swiss-Prot P48453 (PP2BB_MOUSE)

Last modified January 19, 2010. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
    EC=3.1.3.16
Alternative name(s):
    Calmodulin-dependent calcineurin A subunit beta isoform
    CAM-PRP catalytic subunit
Gene names
Name: Ppp3cb
Synonyms: Calnb
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 Fe3+ ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Tissue specificity

Two isoforms in Ehrlich ascites tumor (EAT) is demonstrated by polymerase chain reaction specific primers to the catalytic and calmodulin-binding domain, respectively. Isoform 1 is of medium abundance in EAT cells. Ref.3

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: P48453-1)

Also known as: 2B2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P48453-2)

Also known as: 2B1;

The sequence of this isoform differs from the canonical sequence as follows:
     387-406: MTEGEDQFDVGSAAARKEII → GSEEDGFDGATAAARKEVI

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 525524Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
PRO_0000058826

Regions

Region2 – 310309Catalytic
Region256 – 2627Calcineurin B binding-site 1 Potential
Region305 – 3106Calcineurin B binding-site 2 Potential
Region401 – 42323Calmodulin-binding Potential
Region474 – 49623Inhibitory domain
Compositional bias11 – 2010Poly-Pro

Sites

Active site1601Proton donor By similarity
Metal binding991Iron By similarity
Metal binding1011Iron By similarity
Metal binding1271Iron By similarity
Metal binding1271Zinc By similarity
Metal binding1591Zinc By similarity
Metal binding2081Zinc By similarity
Metal binding2901Zinc By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Natural variations

Alternative sequence387 – 40620MTEGE…RKEII → GSEEDGFDGATAAARKEVI in isoform 1.
VSP_011856

Experimental info

Sequence conflict4811E → G in AAA37411. Ref.2
Sequence conflict523 – 5242SA → TP in AAA37411. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (2B2) [UniParc].

Last modified November 9, 2004. Version 2.
Checksum: 5E73BA3100BE2337

FASTA52559,173
        10         20         30         40         50         60 
MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP RVDVLKNHLV 

        70         80         90        100        110        120 
KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI HGQFFDLMKL FEVGGSPANT 

       130        140        150        160        170        180 
RYLFLGDYVD RGYFSIECVL YLWVLKILYP STLFLLRGNH ECRHLTEYFT FKQECKIKYS 

       190        200        210        220        230        240 
ERVYEACMEA FDSLPLAALL NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL 

       250        260        270        280        290        300 
WSDPSEDFGN EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY 

       310        320        330        340        350        360 
RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY WLPNFMDVFT 

       370        380        390        400        410        420 
WSLPFVGEKV TEMLVNVLSI CSDDELMTEG EDQFDVGSAA ARKEIIRNKI RAIGKMARVF 

       430        440        450        460        470        480 
SVLREESESV LTLKGLTPTG MLPSGVLAGG RQTLQSATVE AIEAEKAIRG FSPPHRICSF 

       490        500        510        520 
EEAKGLDRIN ERMPPRKDAV QQDGFNSLNT AHTTENHGTG NHSAQ

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Isoform 1 (2B1).

Checksum: D5348F544E5ACB35
Show »

FASTA52458,929

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References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[2]"Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit."
Giri P.R., Higuchi S., Kincaid R.L.
Biochem. Biophys. Res. Commun. 181:252-258(1991) [PubMed: 1659808] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-525 (ISOFORM 2).
[3]"Dephosphorylation of the small heat shock protein hsp25 by calcium/calmodulin-dependent (type 2B) protein phosphatase."
Gaestel M., Benndorf R., Hayess K., Priemer E., Engel K.
J. Biol. Chem. 267:21607-21611(1992) [PubMed: 1328240] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-416 (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC066000 mRNA. Translation: AAH66000.1.
M81483 mRNA. Translation: AAA37411.1.
IPIIPI00112312.
IPI00475109.
PIRJT0976.
RefSeqNP_032940.1.
UniGeneMm.274432

3D structure databases

SMRP48453. Positions 24-381.
ModBaseSearch...

Protein-protein interaction databases

IntActP48453. 2 interactions.
STRINGP48453.

PTM databases

PhosphoSiteP48453.

Proteomic databases

PRIDEP48453.

Genome annotation databases

EnsemblENSMUST00000022355; ENSMUSP00000022355; ENSMUSG00000021816; Mus musculus. [Genome view]
GeneID19056.
KEGGmmu:19056.
UCSCuc007sjx.1. mouse.

Organism-specific databases

CTD19056.
MGIMGI:107163. Ppp3cb.

Phylogenomic databases

eggNOGroNOG04245.
HOGENOMHBG716770.
HOVERGENP48453.
InParanoidP48453.
OMAEDQFDGS.
OrthoDBEOG976NK6.
PhylomeDBP48453.

Enzyme and pathway databases

BRENDA3.1.3.16. 244.

Gene expression databases

ArrayExpressP48453.
BgeeP48453.
GenevestigatorP48453.
GermOnlineENSMUSG00000021816. Mus musculus.

Family and domain databases

InterProIPR004843. M-pesterase.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PANTHERPTHR11668. T_phtase_apaH. 1 hit.
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio295546.
SOURCESearch...

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Entry information

Entry namePP2BB_MOUSE
AccessionPrimary (citable) accession number: P48453
Secondary accession number(s): Q6NZR4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 9, 2004
Last modified: January 19, 2010
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents