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P48453

- PP2BB_MOUSE

UniProt

P48453 - PP2BB_MOUSE

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Protein
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
Gene
Ppp3cb, Calnb
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 1 Fe3+ ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron By similarity
Metal bindingi101 – 1011Iron By similarity
Metal bindingi127 – 1271Iron By similarity
Metal bindingi127 – 1271Zinc By similarity
Metal bindingi159 – 1591Zinc By similarity
Active sitei160 – 1601Proton donor By similarity
Metal bindingi208 – 2081Zinc By similarity
Metal bindingi290 – 2901Zinc By similarity

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. calcium-dependent protein serine/threonine phosphatase activity Source: Reactome
  3. calmodulin binding Source: UniProtKB
  4. calmodulin-dependent protein phosphatase activity Source: UniProtKB
  5. drug binding Source: UniProtKB
  6. enzyme binding Source: UniProtKB
  7. protein binding Source: IntAct
  8. protein phosphatase 2B binding Source: UniProtKB
  9. protein serine/threonine phosphatase activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. T cell differentiation Source: MGI
  2. T cell homeostasis Source: MGI
  3. calcium ion-dependent exocytosis Source: UniProtKB
  4. cellular response to drug Source: UniProtKB
  5. heart development Source: MGI
  6. locomotion involved in locomotory behavior Source: MGI
  7. negative regulation of T cell mediated cytotoxicity Source: MGI
  8. positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  9. protein dephosphorylation Source: UniProtKB
  10. protein phosphorylation Source: UniProtKB
  11. regulation of gene expression Source: MGI
  12. regulation of insulin secretion Source: UniProtKB
  13. response to cytokine Source: MGI
  14. social behavior Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_188202. FCERI mediated Ca+2 mobilization.
REACT_203795. DARPP-32 events.
REACT_221970. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (EC:3.1.3.16)
Alternative name(s):
CAM-PRP catalytic subunit
Calmodulin-dependent calcineurin A subunit beta isoform
Gene namesi
Name:Ppp3cb
Synonyms:Calnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:107163. Ppp3cb.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: UniProtKB
  2. cytosol Source: Reactome
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 525524Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
PRO_0000058826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP48453.
PaxDbiP48453.
PRIDEiP48453.

PTM databases

PhosphoSiteiP48453.

Expressioni

Tissue specificityi

Two isoforms in Ehrlich ascites tumor (EAT) is demonstrated by polymerase chain reaction specific primers to the catalytic and calmodulin-binding domain, respectively. Isoform 1 is of medium abundance in EAT cells.1 Publication

Gene expression databases

ArrayExpressiP48453.
BgeeiP48453.
GenevestigatoriP48453.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Akap5D3YVF03EBI-642618,EBI-7091108
Akap6Q9WVC72EBI-642618,EBI-7559840From a different organism.

Protein-protein interaction databases

BioGridi202345. 2 interactions.
IntActiP48453. 8 interactions.
MINTiMINT-1676111.

Structurei

3D structure databases

ProteinModelPortaliP48453.
SMRiP48453. Positions 24-421.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 310309Catalytic
Add
BLAST
Regioni256 – 2627Calcineurin B binding-site 1 Reviewed prediction
Regioni305 – 3106Calcineurin B binding-site 2 Reviewed prediction
Regioni401 – 42323Calmodulin-binding Reviewed prediction
Add
BLAST
Regioni474 – 49623Inhibitory domain
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 2010Poly-Pro

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
HOVERGENiHBG002819.
InParanoidiP48453.
KOiK04348.
OMAiAHTTENH.
OrthoDBiEOG7BZVSC.
TreeFamiTF105557.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 2 (identifier: P48453-1) [UniParc]FASTAAdd to Basket

Also known as: 2B2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP    50
RVDVLKNHLV KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI 100
HGQFFDLMKL FEVGGSPANT RYLFLGDYVD RGYFSIECVL YLWVLKILYP 150
STLFLLRGNH ECRHLTEYFT FKQECKIKYS ERVYEACMEA FDSLPLAALL 200
NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL WSDPSEDFGN 250
EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY 300
RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY 350
WLPNFMDVFT WSLPFVGEKV TEMLVNVLSI CSDDELMTEG EDQFDVGSAA 400
ARKEIIRNKI RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLAGG 450
RQTLQSATVE AIEAEKAIRG FSPPHRICSF EEAKGLDRIN ERMPPRKDAV 500
QQDGFNSLNT AHTTENHGTG NHSAQ 525
Length:525
Mass (Da):59,173
Last modified:November 9, 2004 - v2
Checksum:i5E73BA3100BE2337
GO
Isoform 1 (identifier: P48453-2) [UniParc]FASTAAdd to Basket

Also known as: 2B1

The sequence of this isoform differs from the canonical sequence as follows:
     387-406: MTEGEDQFDVGSAAARKEII → GSEEDGFDGATAAARKEVI

Show »
Length:524
Mass (Da):58,929
Checksum:iD5348F544E5ACB35
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei387 – 40620MTEGE…RKEII → GSEEDGFDGATAAARKEVI in isoform 1.
VSP_011856Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti481 – 4811E → G in AAA37411. 1 Publication
Sequence conflicti523 – 5242SA → TP in AAA37411. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC066000 mRNA. Translation: AAH66000.1.
M81483 mRNA. Translation: AAA37411.1.
CCDSiCCDS26846.1. [P48453-1]
PIRiJT0976.
RefSeqiNP_032940.1. NM_008914.2. [P48453-1]
UniGeneiMm.274432.

Genome annotation databases

EnsembliENSMUST00000159027; ENSMUSP00000125722; ENSMUSG00000021816. [P48453-1]
GeneIDi19056.
KEGGimmu:19056.
UCSCiuc007sjx.2. mouse. [P48453-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC066000 mRNA. Translation: AAH66000.1 .
M81483 mRNA. Translation: AAA37411.1 .
CCDSi CCDS26846.1. [P48453-1 ]
PIRi JT0976.
RefSeqi NP_032940.1. NM_008914.2. [P48453-1 ]
UniGenei Mm.274432.

3D structure databases

ProteinModelPortali P48453.
SMRi P48453. Positions 24-421.
ModBasei Search...

Protein-protein interaction databases

BioGridi 202345. 2 interactions.
IntActi P48453. 8 interactions.
MINTi MINT-1676111.

PTM databases

PhosphoSitei P48453.

Proteomic databases

MaxQBi P48453.
PaxDbi P48453.
PRIDEi P48453.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000159027 ; ENSMUSP00000125722 ; ENSMUSG00000021816 . [P48453-1 ]
GeneIDi 19056.
KEGGi mmu:19056.
UCSCi uc007sjx.2. mouse. [P48453-1 ]

Organism-specific databases

CTDi 5532.
MGIi MGI:107163. Ppp3cb.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00530000063087.
HOGENOMi HOG000172699.
HOVERGENi HBG002819.
InParanoidi P48453.
KOi K04348.
OMAi AHTTENH.
OrthoDBi EOG7BZVSC.
TreeFami TF105557.

Enzyme and pathway databases

Reactomei REACT_188202. FCERI mediated Ca+2 mobilization.
REACT_203795. DARPP-32 events.
REACT_221970. Ca2+ pathway.

Miscellaneous databases

ChiTaRSi PPP3CB. mouse.
NextBioi 295546.
PROi P48453.
SOURCEi Search...

Gene expression databases

ArrayExpressi P48453.
Bgeei P48453.
Genevestigatori P48453.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit."
    Giri P.R., Higuchi S., Kincaid R.L.
    Biochem. Biophys. Res. Commun. 181:252-258(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-525 (ISOFORM 2).
  3. "Dephosphorylation of the small heat shock protein hsp25 by calcium/calmodulin-dependent (type 2B) protein phosphatase."
    Gaestel M., Benndorf R., Hayess K., Priemer E., Engel K.
    J. Biol. Chem. 267:21607-21611(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-416 (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.

Entry informationi

Entry nameiPP2BB_MOUSE
AccessioniPrimary (citable) accession number: P48453
Secondary accession number(s): Q6NZR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 9, 2004
Last modified: September 3, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi