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P48453

- PP2BB_MOUSE

UniProt

P48453 - PP2BB_MOUSE

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform

Gene

Ppp3cb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (09 Nov 2004)
      Previous versions | rss
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    Functioni

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 Fe3+ ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991IronBy similarity
    Metal bindingi101 – 1011IronBy similarity
    Metal bindingi127 – 1271IronBy similarity
    Metal bindingi127 – 1271ZincBy similarity
    Metal bindingi159 – 1591ZincBy similarity
    Active sitei160 – 1601Proton donorBy similarity
    Metal bindingi208 – 2081ZincBy similarity
    Metal bindingi290 – 2901ZincBy similarity

    GO - Molecular functioni

    1. calcium-dependent protein serine/threonine phosphatase activity Source: Reactome
    2. calcium ion binding Source: UniProtKB
    3. calmodulin binding Source: UniProtKB
    4. calmodulin-dependent protein phosphatase activity Source: UniProtKB
    5. drug binding Source: UniProtKB
    6. enzyme binding Source: UniProtKB
    7. protein binding Source: IntAct
    8. protein phosphatase 2B binding Source: UniProtKB
    9. protein serine/threonine phosphatase activity Source: MGI

    GO - Biological processi

    1. calcium ion-dependent exocytosis Source: UniProtKB
    2. cellular response to drug Source: UniProtKB
    3. heart development Source: MGI
    4. locomotion involved in locomotory behavior Source: MGI
    5. negative regulation of T cell mediated cytotoxicity Source: MGI
    6. positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
    7. protein dephosphorylation Source: UniProtKB
    8. protein phosphorylation Source: UniProtKB
    9. regulation of gene expression Source: MGI
    10. regulation of insulin secretion Source: UniProtKB
    11. response to cytokine Source: MGI
    12. social behavior Source: Ensembl
    13. T cell differentiation Source: MGI
    14. T cell homeostasis Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Calmodulin-binding, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_203795. DARPP-32 events.
    REACT_221970. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (EC:3.1.3.16)
    Alternative name(s):
    CAM-PRP catalytic subunit
    Calmodulin-dependent calcineurin A subunit beta isoform
    Gene namesi
    Name:Ppp3cb
    Synonyms:Calnb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:107163. Ppp3cb.

    Subcellular locationi

    GO - Cellular componenti

    1. calcineurin complex Source: UniProtKB
    2. cytosol Source: Reactome
    3. plasma membrane Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 525524Serine/threonine-protein phosphatase 2B catalytic subunit beta isoformPRO_0000058826Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP48453.
    PaxDbiP48453.
    PRIDEiP48453.

    PTM databases

    PhosphoSiteiP48453.

    Expressioni

    Tissue specificityi

    Two isoforms in Ehrlich ascites tumor (EAT) is demonstrated by polymerase chain reaction specific primers to the catalytic and calmodulin-binding domain, respectively. Isoform 1 is of medium abundance in EAT cells.1 Publication

    Gene expression databases

    ArrayExpressiP48453.
    BgeeiP48453.
    GenevestigatoriP48453.

    Interactioni

    Subunit structurei

    Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Akap5D3YVF03EBI-642618,EBI-7091108
    Akap6Q9WVC72EBI-642618,EBI-7559840From a different organism.

    Protein-protein interaction databases

    BioGridi202345. 2 interactions.
    IntActiP48453. 8 interactions.
    MINTiMINT-1676111.

    Structurei

    3D structure databases

    ProteinModelPortaliP48453.
    SMRiP48453. Positions 24-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 310309CatalyticAdd
    BLAST
    Regioni256 – 2627Calcineurin B binding-site 1Sequence Analysis
    Regioni305 – 3106Calcineurin B binding-site 2Sequence Analysis
    Regioni401 – 42323Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni474 – 49623Inhibitory domainAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 2010Poly-Pro

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063087.
    HOGENOMiHOG000172699.
    HOVERGENiHBG002819.
    InParanoidiP48453.
    KOiK04348.
    OMAiAHTTENH.
    OrthoDBiEOG7BZVSC.
    TreeFamiTF105557.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: P48453-1) [UniParc]FASTAAdd to Basket

    Also known as: 2B2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP    50
    RVDVLKNHLV KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI 100
    HGQFFDLMKL FEVGGSPANT RYLFLGDYVD RGYFSIECVL YLWVLKILYP 150
    STLFLLRGNH ECRHLTEYFT FKQECKIKYS ERVYEACMEA FDSLPLAALL 200
    NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL WSDPSEDFGN 250
    EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY 300
    RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY 350
    WLPNFMDVFT WSLPFVGEKV TEMLVNVLSI CSDDELMTEG EDQFDVGSAA 400
    ARKEIIRNKI RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLAGG 450
    RQTLQSATVE AIEAEKAIRG FSPPHRICSF EEAKGLDRIN ERMPPRKDAV 500
    QQDGFNSLNT AHTTENHGTG NHSAQ 525
    Length:525
    Mass (Da):59,173
    Last modified:November 9, 2004 - v2
    Checksum:i5E73BA3100BE2337
    GO
    Isoform 1 (identifier: P48453-2) [UniParc]FASTAAdd to Basket

    Also known as: 2B1

    The sequence of this isoform differs from the canonical sequence as follows:
         387-406: MTEGEDQFDVGSAAARKEII → GSEEDGFDGATAAARKEVI

    Show »
    Length:524
    Mass (Da):58,929
    Checksum:iD5348F544E5ACB35
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti481 – 4811E → G in AAA37411. (PubMed:1659808)Curated
    Sequence conflicti523 – 5242SA → TP in AAA37411. (PubMed:1659808)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei387 – 40620MTEGE…RKEII → GSEEDGFDGATAAARKEVI in isoform 1. 1 PublicationVSP_011856Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC066000 mRNA. Translation: AAH66000.1.
    M81483 mRNA. Translation: AAA37411.1.
    CCDSiCCDS26846.1. [P48453-1]
    PIRiJT0976.
    RefSeqiNP_032940.1. NM_008914.2. [P48453-1]
    UniGeneiMm.274432.

    Genome annotation databases

    EnsembliENSMUST00000159027; ENSMUSP00000125722; ENSMUSG00000021816. [P48453-1]
    GeneIDi19056.
    KEGGimmu:19056.
    UCSCiuc007sjx.2. mouse. [P48453-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC066000 mRNA. Translation: AAH66000.1 .
    M81483 mRNA. Translation: AAA37411.1 .
    CCDSi CCDS26846.1. [P48453-1 ]
    PIRi JT0976.
    RefSeqi NP_032940.1. NM_008914.2. [P48453-1 ]
    UniGenei Mm.274432.

    3D structure databases

    ProteinModelPortali P48453.
    SMRi P48453. Positions 24-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202345. 2 interactions.
    IntActi P48453. 8 interactions.
    MINTi MINT-1676111.

    PTM databases

    PhosphoSitei P48453.

    Proteomic databases

    MaxQBi P48453.
    PaxDbi P48453.
    PRIDEi P48453.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000159027 ; ENSMUSP00000125722 ; ENSMUSG00000021816 . [P48453-1 ]
    GeneIDi 19056.
    KEGGi mmu:19056.
    UCSCi uc007sjx.2. mouse. [P48453-1 ]

    Organism-specific databases

    CTDi 5532.
    MGIi MGI:107163. Ppp3cb.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063087.
    HOGENOMi HOG000172699.
    HOVERGENi HBG002819.
    InParanoidi P48453.
    KOi K04348.
    OMAi AHTTENH.
    OrthoDBi EOG7BZVSC.
    TreeFami TF105557.

    Enzyme and pathway databases

    Reactomei REACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_203795. DARPP-32 events.
    REACT_221970. Ca2+ pathway.

    Miscellaneous databases

    ChiTaRSi PPP3CB. mouse.
    NextBioi 295546.
    PROi P48453.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48453.
    Bgeei P48453.
    Genevestigatori P48453.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit."
      Giri P.R., Higuchi S., Kincaid R.L.
      Biochem. Biophys. Res. Commun. 181:252-258(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-525 (ISOFORM 2).
    3. "Dephosphorylation of the small heat shock protein hsp25 by calcium/calmodulin-dependent (type 2B) protein phosphatase."
      Gaestel M., Benndorf R., Hayess K., Priemer E., Engel K.
      J. Biol. Chem. 267:21607-21611(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-416 (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPP2BB_MOUSE
    AccessioniPrimary (citable) accession number: P48453
    Secondary accession number(s): Q6NZR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: November 9, 2004
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3