P48453 (PP2BB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
EC=3.1.3.16

Alternative name(s):
CAM-PRP catalytic subunit
Calmodulin-dependent calcineurin A subunit beta isoform

Gene names

Name:	Ppp3cb
Synonyms:	Calnb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 Fe3+ ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.
Tissue specificity	Two isoforms in Ehrlich ascites tumor (EAT) is demonstrated by polymerase chain reaction specific primers to the catalytic and calmodulin-binding domain, respectively. Isoform 1 is of medium abundance in EAT cells. Ref.3
Sequence similarities	Belongs to the PPP phosphatase family. PP-2B subfamily.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Calmodulin-binding Iron Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell differentiation Inferred from mutant phenotype PubMed 12091710. Source: MGI T cell homeostasis Inferred from mutant phenotype PubMed 18097009. Source: MGI calcium ion-dependent exocytosis Inferred from direct assay PubMed 11978799. Source: UniProtKB cellular response to drug Inferred from sequence or structural similarity. Source: UniProtKB heart development Inferred from mutant phenotype PubMed 11904392. Source: MGI negative regulation of T cell mediated cytotoxicity Inferred from mutant phenotype PubMed 18097009. Source: MGI positive regulation of insulin secretion involved in cellular response to glucose stimulus Inferred from mutant phenotype PubMed 11978799. Source: UniProtKB protein dephosphorylation Inferred from mutant phenotype PubMed 11978799. Source: UniProtKB protein phosphorylation Inferred from mutant phenotype PubMed 11978799. Source: UniProtKB regulation of gene expression Inferred from mutant phenotype PubMed 18097009. Source: MGI response to cytokine stimulus Inferred from mutant phenotype PubMed 18097009. Source: MGI social behavior Inferred from electronic annotation. Source: Compara
Cellular_component	calcineurin complex Inferred from sequence or structural similarity. Source: UniProtKB cytosol Traceable author statement. Source: Reactome plasma membrane Inferred from direct assay PubMed 11978799. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB calmodulin binding Inferred from sequence or structural similarity. Source: UniProtKB calmodulin-dependent protein phosphatase activity Inferred from mutant phenotype PubMed 11978799. Source: UniProtKB drug binding Inferred from sequence or structural similarity. Source: UniProtKB protein phosphatase 2B binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: P48453-1)

Also known as: 2B2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P48453-2)

Also known as: 2B1;

The sequence of this isoform differs from the canonical sequence as follows:
     387-406: MTEGEDQFDVGSAAARKEII → GSEEDGFDGATAAARKEVI

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform		PRO_0000058826
Regions
	Region	1 – 310	310	Catalytic
	Region	256 – 262	7	Calcineurin B binding-site 1 Potential
	Region	305 – 310	6	Calcineurin B binding-site 2 Potential
	Region	401 – 423	23	Calmodulin-binding Potential
	Region	474 – 496	23	Inhibitory domain
	Compositional bias	11 – 20	10	Poly-Pro
Sites
	Active site	160	1	Proton donor By similarity
	Metal binding	99	1	Iron By similarity
	Metal binding	101	1	Iron By similarity
	Metal binding	127	1	Iron By similarity
	Metal binding	127	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	208	1	Zinc By similarity
	Metal binding	290	1	Zinc By similarity
Natural variations
	Alternative sequence	387 – 406	20	MTEGE…RKEII → GSEEDGFDGATAAARKEVI in isoform 1.		VSP_011856
Experimental info
	Sequence conflict	481	1	E → G in AAA37411. Ref.2
	Sequence conflict	523 – 524	2	SA → TP in AAA37411. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 (2B2) [UniParc]. Last modified November 9, 2004. Version 2. Checksum: 5E73BA3100BE2337	FASTA	525	59,173
	10 20 30 40 50 60 MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP RVDVLKNHLV 70 80 90 100 110 120 KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI HGQFFDLMKL FEVGGSPANT 130 140 150 160 170 180 RYLFLGDYVD RGYFSIECVL YLWVLKILYP STLFLLRGNH ECRHLTEYFT FKQECKIKYS 190 200 210 220 230 240 ERVYEACMEA FDSLPLAALL NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL 250 260 270 280 290 300 WSDPSEDFGN EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY 310 320 330 340 350 360 RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY WLPNFMDVFT 370 380 390 400 410 420 WSLPFVGEKV TEMLVNVLSI CSDDELMTEG EDQFDVGSAA ARKEIIRNKI RAIGKMARVF 430 440 450 460 470 480 SVLREESESV LTLKGLTPTG MLPSGVLAGG RQTLQSATVE AIEAEKAIRG FSPPHRICSF 490 500 510 520 EEAKGLDRIN ERMPPRKDAV QQDGFNSLNT AHTTENHGTG NHSAQ
	Isoform 1 (2B1) [UniParc]. Checksum: D5348F544E5ACB35	FASTA	524	58,929
	10 20 30 40 50 60 MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP RVDVLKNHLV 70 80 90 100 110 120 KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI HGQFFDLMKL FEVGGSPANT 130 140 150 160 170 180 RYLFLGDYVD RGYFSIECVL YLWVLKILYP STLFLLRGNH ECRHLTEYFT FKQECKIKYS 190 200 210 220 230 240 ERVYEACMEA FDSLPLAALL NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL 250 260 270 280 290 300 WSDPSEDFGN EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY 310 320 330 340 350 360 RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY WLPNFMDVFT 370 380 390 400 410 420 WSLPFVGEKV TEMLVNVLSI CSDDELGSEE DGFDGATAAA RKEVIRNKIR AIGKMARVFS 430 440 450 460 470 480 VLREESESVL TLKGLTPTGM LPSGVLAGGR QTLQSATVEA IEAEKAIRGF SPPHRICSFE 490 500 510 520 EAKGLDRINE RMPPRKDAVQ QDGFNSLNTA HTTENHGTGN HSAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[2]	"Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit." Giri P.R., Higuchi S., Kincaid R.L. Biochem. Biophys. Res. Commun. 181:252-258(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-525 (ISOFORM 2).
[3]	"Dephosphorylation of the small heat shock protein hsp25 by calcium/calmodulin-dependent (type 2B) protein phosphatase." Gaestel M., Benndorf R., Hayess K., Priemer E., Engel K. J. Biol. Chem. 267:21607-21611(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-416 (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC066000 mRNA. Translation: AAH66000.1. M81483 mRNA. Translation: AAA37411.1.
IPI	IPI00112312. IPI00475109.
PIR	JT0976.
RefSeq	NP_032940.1. NM_008914.2.
UniGene	Mm.274432.
3D structure databases
ProteinModelPortal	P48453.
SMR	P48453. Positions 24-421.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48453. 2 interactions.
PTM databases
PhosphoSite	P48453.
Proteomic databases
PaxDb	P48453.
PRIDE	P48453.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000159027; ENSMUSP00000125722; ENSMUSG00000021816.
GeneID	19056.
KEGG	mmu:19056.
UCSC	uc007sjx.2. mouse.
Organism-specific databases
CTD	5532.
MGI	MGI:107163. Ppp3cb.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	ENSGT00530000063087.
HOGENOM	HOG000172699.
HOVERGEN	HBG002819.
InParanoid	P48453.
KO	K04348.
OMA	DQFDVKV.
OrthoDB	EOG4PVNZK.
Enzyme and pathway databases
Reactome	REACT_118809. Calcineurin Dephosphorylates Nfatc2.
Gene expression databases
ArrayExpress	P48453.
Bgee	P48453.
Genevestigator	P48453.
GermOnline	ENSMUSG00000021816. Mus musculus.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PPP3CB. mouse.
NextBio	295546.
SOURCE	Search...

Entry information

Entry name

PP2BB_MOUSE

Accession

Primary (citable) accession number: P48453
Secondary accession number(s): Q6NZR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 9, 2004
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program