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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform

Gene

Ppp3cb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991IronBy similarity
Metal bindingi101 – 1011IronBy similarity
Metal bindingi127 – 1271IronBy similarity
Metal bindingi127 – 1271ZincBy similarity
Metal bindingi159 – 1591ZincBy similarity
Active sitei160 – 1601Proton donorBy similarity
Metal bindingi208 – 2081ZincBy similarity
Metal bindingi290 – 2901ZincBy similarity

GO - Molecular functioni

GO - Biological processi

  • calcineurin-NFAT signaling cascade Source: MGI
  • calcium ion-dependent exocytosis Source: UniProtKB
  • cellular response to drug Source: UniProtKB
  • heart development Source: MGI
  • locomotion involved in locomotory behavior Source: MGI
  • lymphangiogenesis Source: MGI
  • negative regulation of T cell mediated cytotoxicity Source: MGI
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • positive regulation of NFAT protein import into nucleus Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein dephosphorylation Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • regulation of gene expression Source: MGI
  • regulation of insulin secretion Source: UniProtKB
  • response to cytokine Source: MGI
  • social behavior Source: Ensembl
  • T cell differentiation Source: MGI
  • T cell homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_325290. DARPP-32 events.
REACT_331940. Ca2+ pathway.
REACT_348733. FCERI mediated Ca+2 mobilization.
REACT_362087. CLEC7A (Dectin-1) induces NFAT activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (EC:3.1.3.16)
Alternative name(s):
CAM-PRP catalytic subunit
Calmodulin-dependent calcineurin A subunit beta isoform
Gene namesi
Name:Ppp3cb
Synonyms:Calnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:107163. Ppp3cb.

Subcellular locationi

GO - Cellular componenti

  • calcineurin complex Source: UniProtKB
  • cytosol Source: Reactome
  • plasma membrane Source: UniProtKB
  • T-tubule Source: BHF-UCL
  • Z disc Source: BHF-UCL
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 525524Serine/threonine-protein phosphatase 2B catalytic subunit beta isoformPRO_0000058826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP48453.
PaxDbiP48453.
PRIDEiP48453.

PTM databases

PhosphoSiteiP48453.

Expressioni

Tissue specificityi

Two isoforms in Ehrlich ascites tumor (EAT) is demonstrated by polymerase chain reaction specific primers to the catalytic and calmodulin-binding domain, respectively. Isoform 1 is of medium abundance in EAT cells.1 Publication

Gene expression databases

BgeeiP48453.
ExpressionAtlasiP48453. baseline and differential.
GenevestigatoriP48453.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Akap5D3YVF03EBI-642618,EBI-7091108
Akap6Q9WVC72EBI-642618,EBI-7559840From a different organism.

Protein-protein interaction databases

BioGridi202345. 2 interactions.
IntActiP48453. 8 interactions.
MINTiMINT-1676111.

Structurei

3D structure databases

ProteinModelPortaliP48453.
SMRiP48453. Positions 24-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 310309CatalyticAdd
BLAST
Regioni256 – 2627Calcineurin B binding-site 1Sequence Analysis
Regioni305 – 3106Calcineurin B binding-site 2Sequence Analysis
Regioni401 – 42323Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni474 – 49623Inhibitory domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 2010Poly-Pro

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
HOVERGENiHBG002819.
InParanoidiP48453.
KOiK04348.
OMAiAHTTENH.
OrthoDBiEOG7BZVSC.
TreeFamiTF105557.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P48453-1) [UniParc]FASTAAdd to basket

Also known as: 2B2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPEPARAA PPPPPPPPPP LGADRVVKAV PFPPTHRLTS EEVFDMDGIP
60 70 80 90 100
RVDVLKNHLV KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI
110 120 130 140 150
HGQFFDLMKL FEVGGSPANT RYLFLGDYVD RGYFSIECVL YLWVLKILYP
160 170 180 190 200
STLFLLRGNH ECRHLTEYFT FKQECKIKYS ERVYEACMEA FDSLPLAALL
210 220 230 240 250
NQQFLCVHGG LSPEIHTLDD IRRLDRFKEP PAFGPMCDLL WSDPSEDFGN
260 270 280 290 300
EKSQEHFSHN TVRGCSYFYN YPAVCEFLQN NNLLSIIRAH EAQDAGYRMY
310 320 330 340 350
RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY
360 370 380 390 400
WLPNFMDVFT WSLPFVGEKV TEMLVNVLSI CSDDELMTEG EDQFDVGSAA
410 420 430 440 450
ARKEIIRNKI RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLAGG
460 470 480 490 500
RQTLQSATVE AIEAEKAIRG FSPPHRICSF EEAKGLDRIN ERMPPRKDAV
510 520
QQDGFNSLNT AHTTENHGTG NHSAQ
Length:525
Mass (Da):59,173
Last modified:November 9, 2004 - v2
Checksum:i5E73BA3100BE2337
GO
Isoform 1 (identifier: P48453-2) [UniParc]FASTAAdd to basket

Also known as: 2B1

The sequence of this isoform differs from the canonical sequence as follows:
     387-406: MTEGEDQFDVGSAAARKEII → GSEEDGFDGATAAARKEVI

Show »
Length:524
Mass (Da):58,929
Checksum:iD5348F544E5ACB35
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti481 – 4811E → G in AAA37411 (PubMed:1659808).Curated
Sequence conflicti523 – 5242SA → TP in AAA37411 (PubMed:1659808).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei387 – 40620MTEGE…RKEII → GSEEDGFDGATAAARKEVI in isoform 1. 1 PublicationVSP_011856Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC066000 mRNA. Translation: AAH66000.1.
M81483 mRNA. Translation: AAA37411.1.
CCDSiCCDS26846.1. [P48453-1]
PIRiJT0976.
RefSeqiNP_032940.1. NM_008914.2. [P48453-1]
UniGeneiMm.274432.

Genome annotation databases

EnsembliENSMUST00000159027; ENSMUSP00000125722; ENSMUSG00000021816. [P48453-1]
GeneIDi19056.
KEGGimmu:19056.
UCSCiuc007sjx.2. mouse. [P48453-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC066000 mRNA. Translation: AAH66000.1.
M81483 mRNA. Translation: AAA37411.1.
CCDSiCCDS26846.1. [P48453-1]
PIRiJT0976.
RefSeqiNP_032940.1. NM_008914.2. [P48453-1]
UniGeneiMm.274432.

3D structure databases

ProteinModelPortaliP48453.
SMRiP48453. Positions 24-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202345. 2 interactions.
IntActiP48453. 8 interactions.
MINTiMINT-1676111.

PTM databases

PhosphoSiteiP48453.

Proteomic databases

MaxQBiP48453.
PaxDbiP48453.
PRIDEiP48453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000159027; ENSMUSP00000125722; ENSMUSG00000021816. [P48453-1]
GeneIDi19056.
KEGGimmu:19056.
UCSCiuc007sjx.2. mouse. [P48453-1]

Organism-specific databases

CTDi5532.
MGIiMGI:107163. Ppp3cb.

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
HOVERGENiHBG002819.
InParanoidiP48453.
KOiK04348.
OMAiAHTTENH.
OrthoDBiEOG7BZVSC.
TreeFamiTF105557.

Enzyme and pathway databases

ReactomeiREACT_325290. DARPP-32 events.
REACT_331940. Ca2+ pathway.
REACT_348733. FCERI mediated Ca+2 mobilization.
REACT_362087. CLEC7A (Dectin-1) induces NFAT activation.

Miscellaneous databases

ChiTaRSiPpp3cb. mouse.
NextBioi295546.
PROiP48453.
SOURCEiSearch...

Gene expression databases

BgeeiP48453.
ExpressionAtlasiP48453. baseline and differential.
GenevestigatoriP48453.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit."
    Giri P.R., Higuchi S., Kincaid R.L.
    Biochem. Biophys. Res. Commun. 181:252-258(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-525 (ISOFORM 2).
  3. "Dephosphorylation of the small heat shock protein hsp25 by calcium/calmodulin-dependent (type 2B) protein phosphatase."
    Gaestel M., Benndorf R., Hayess K., Priemer E., Engel K.
    J. Biol. Chem. 267:21607-21611(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 320-416 (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.

Entry informationi

Entry nameiPP2BB_MOUSE
AccessioniPrimary (citable) accession number: P48453
Secondary accession number(s): Q6NZR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 9, 2004
Last modified: May 27, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.