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P48452

- PP2BA_BOVIN

UniProt

P48452 - PP2BA_BOVIN

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Gene

PPP3CA

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1 By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 Fe3+ ion per subunit.
    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi90 – 901Iron1 Publication
    Metal bindingi92 – 921Iron1 Publication
    Metal bindingi118 – 1181Iron1 Publication
    Metal bindingi118 – 1181Zinc1 Publication
    Metal bindingi150 – 1501Zinc1 Publication
    Active sitei151 – 1511Proton donorBy similarity
    Metal bindingi199 – 1991Zinc1 Publication
    Metal bindingi281 – 2811Zinc1 Publication

    GO - Molecular functioni

    1. calmodulin-dependent protein phosphatase activity Source: UniProtKB
    2. drug binding Source: Ensembl
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. calcineurin-NFAT signaling cascade Source: Ensembl
    2. calcium ion transport Source: Ensembl
    3. cellular response to drug Source: Ensembl
    4. dephosphorylation Source: UniProtKB
    5. G1/S transition of mitotic cell cycle Source: Ensembl
    6. multicellular organismal response to stress Source: Ensembl
    7. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    8. protein dephosphorylation Source: UniProtKB
    9. protein import into nucleus Source: Ensembl
    10. regulation of excitatory postsynaptic membrane potential Source: Ensembl
    11. regulation of synaptic transmission Source: Ensembl
    12. response to calcium ion Source: UniProtKB
    13. skeletal muscle fiber development Source: Ensembl
    14. transition between fast and slow fiber Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Calmodulin-binding, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_205490. DARPP-32 events.
    REACT_209727. Ca2+ pathway.
    REACT_216829. FCERI mediated Ca+2 mobilization.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (EC:3.1.3.16)
    Alternative name(s):
    CAM-PRP catalytic subunit
    Calmodulin-dependent calcineurin A subunit alpha isoform
    Gene namesi
    Name:PPP3CA
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 6

    Subcellular locationi

    Cell membrane By similarity. Cell membranesarcolemma By similarity. Nucleus By similarity
    Note: Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.By similarity

    GO - Cellular componenti

    1. calcineurin complex Source: Ensembl
    2. cytosol Source: Reactome
    3. mitochondrion Source: Ensembl
    4. nucleus Source: UniProtKB-SubCell
    5. sarcolemma Source: UniProtKB-SubCell
    6. Z disc Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 521520Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoformPRO_0000058821Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei224 – 2241Nitrated tyrosineBy similarity

    Keywords - PTMi

    Acetylation, Nitration

    Proteomic databases

    PRIDEiP48452.

    Interactioni

    Subunit structurei

    Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria. Interacts with CHP1 and CHP2 By similarity. Interacts with CMYA5; this interaction represses calcineurin activity in muscle By similarity. Interacts with CIB1; the interaction increases upon cardiomyocytes hypertrophy By similarity.By similarity

    Protein-protein interaction databases

    IntActiP48452. 1 interaction.

    Structurei

    Secondary structure

    1
    521
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi31 – 344
    Helixi43 – 519
    Helixi58 – 7417
    Beta strandi77 – 815
    Beta strandi83 – 886
    Helixi95 – 10511
    Turni108 – 1103
    Beta strandi113 – 1153
    Beta strandi120 – 1234
    Helixi126 – 13914
    Turni141 – 1433
    Beta strandi144 – 1463
    Helixi154 – 1596
    Helixi162 – 1698
    Helixi172 – 18211
    Beta strandi188 – 1914
    Turni192 – 1943
    Beta strandi195 – 1973
    Helixi209 – 2135
    Beta strandi218 – 2203
    Beta strandi223 – 2253
    Helixi226 – 2327
    Beta strandi239 – 2413
    Beta strandi247 – 2504
    Turni252 – 2554
    Beta strandi256 – 2605
    Helixi262 – 27211
    Beta strandi275 – 2795
    Beta strandi287 – 2904
    Beta strandi295 – 30511
    Helixi311 – 3133
    Beta strandi319 – 3257
    Beta strandi328 – 3347
    Helixi344 – 3463
    Helixi349 – 36921

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TCOX-ray2.50A18-392[»]
    2F2OX-ray2.17A/B389-413[»]
    2F2PX-ray2.60A/B389-413[»]
    ProteinModelPortaliP48452.
    SMRiP48452. Positions 21-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP48452.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 301300CatalyticAdd
    BLAST
    Regioni247 – 2537Calcineurin B binding-site 1Sequence Analysis
    Regioni296 – 3016Calcineurin B binding-site 2Sequence Analysis
    Regioni392 – 41423Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni465 – 48723Inhibitory domainAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063087.
    HOGENOMiHOG000172699.
    HOVERGENiHBG002819.
    InParanoidiP48452.
    KOiK04348.
    OMAiHALFILE.
    OrthoDBiEOG7BZVSC.
    TreeFamiTF105557.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P48452-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL    50
    MKEGRLEETV ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK 100
    LFEVGGSPAN TRYLFLGDYV DRGYFSIECV LYLWALKILY PKTLFLLRGN 150
    HECRHLTEYF TFKQECKIKY SERVYDACMD AFDCLPLAAL MNQQFLCVHG 200
    GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG NEKTQEHFTH 250
    NTVRGCSYFY SYPAVCEFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP 300
    SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF 350
    TWSLPFVGEK VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI 400
    RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLSGG KQTLQSATVE 450
    AIEADEAIKG FSPQHKITSF EEAKGLDRIN ERMPPRRDAM PSDANLNSIN 500
    KALASETNGT DSNGSNSSNI Q 521
    Length:521
    Mass (Da):58,672
    Last modified:February 1, 1996 - v1
    Checksum:iF3F153F22AB56BDF
    GO
    Isoform 2 (identifier: P48452-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         448-457: Missing.

    Show »
    Length:511
    Mass (Da):57,643
    Checksum:iC383F9EF7B41D775
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti367 – 3671N → D in ABB22788. (PubMed:15967565)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei448 – 45710Missing in isoform 2. 1 PublicationVSP_018561

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33868 mRNA. Translation: AAC48473.1.
    DQ231569 mRNA. Translation: ABB22788.1.
    BC123668 mRNA. Translation: AAI23669.1.
    PIRiA56968.
    RefSeqiNP_777212.1. NM_174787.2. [P48452-1]
    XP_005207750.1. XM_005207693.1. [P48452-2]
    UniGeneiBt.3966.

    Genome annotation databases

    EnsembliENSBTAT00000021305; ENSBTAP00000021305; ENSBTAG00000016005. [P48452-1]
    ENSBTAT00000056385; ENSBTAP00000047848; ENSBTAG00000016005. [P48452-2]
    GeneIDi286852.
    KEGGibta:286852.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33868 mRNA. Translation: AAC48473.1 .
    DQ231569 mRNA. Translation: ABB22788.1 .
    BC123668 mRNA. Translation: AAI23669.1 .
    PIRi A56968.
    RefSeqi NP_777212.1. NM_174787.2. [P48452-1 ]
    XP_005207750.1. XM_005207693.1. [P48452-2 ]
    UniGenei Bt.3966.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TCO X-ray 2.50 A 18-392 [» ]
    2F2O X-ray 2.17 A/B 389-413 [» ]
    2F2P X-ray 2.60 A/B 389-413 [» ]
    ProteinModelPortali P48452.
    SMRi P48452. Positions 21-372.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48452. 1 interaction.

    Proteomic databases

    PRIDEi P48452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000021305 ; ENSBTAP00000021305 ; ENSBTAG00000016005 . [P48452-1 ]
    ENSBTAT00000056385 ; ENSBTAP00000047848 ; ENSBTAG00000016005 . [P48452-2 ]
    GeneIDi 286852.
    KEGGi bta:286852.

    Organism-specific databases

    CTDi 5530.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063087.
    HOGENOMi HOG000172699.
    HOVERGENi HBG002819.
    InParanoidi P48452.
    KOi K04348.
    OMAi HALFILE.
    OrthoDBi EOG7BZVSC.
    TreeFami TF105557.

    Enzyme and pathway databases

    Reactomei REACT_205490. DARPP-32 events.
    REACT_209727. Ca2+ pathway.
    REACT_216829. FCERI mediated Ca+2 mobilization.

    Miscellaneous databases

    EvolutionaryTracei P48452.
    NextBioi 20806501.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12-FK506 complex."
      Griffith J.P., Kim J.L., Kim E.E., Sintchak M.D., Thomson J.A., Fitzgibbon M.J., Fleming M.A., Caron P.R., Hsiao K., Navia M.A.
      Cell 82:507-522(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH IRON AND ZINC.
      Tissue: Brain.
    2. "Molecular cloning, expression, purification and characterization of calcineurin from bovine cardiac muscle."
      Selvakumar P., Lakshmikuttyamma A., Anderson D.H., Sharma R.K.
      Biochimie 87:975-983(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Heart muscle.
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Hereford.
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiPP2BA_BOVIN
    AccessioniPrimary (citable) accession number: P48452
    Secondary accession number(s): Q08DM4, Q309F2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3