Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P48445

- BPL1_YEAST

UniProt

P48445 - BPL1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Biotin--protein ligase

Gene

BPL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase.

Catalytic activityi

ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxytransferase] = AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxytransferase].
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. biotin-[acetyl-CoA-carboxylase] ligase activity Source: SGD
  3. biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity Source: UniProtKB-EC
  4. biotin-[methylmalonyl-CoA-carboxytransferase] ligase activity Source: UniProtKB-EC
  5. biotin-[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase activity Source: UniProtKB-EC
  6. biotin-[pyruvate-carboxylase] ligase activity Source: SGD

GO - Biological processi

  1. protein biotinylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YDL141W-MONOMER.
ReactomeiREACT_188774. Biotin transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin--protein ligase (EC:6.3.4.-)
Alternative name(s):
Biotin apo-protein ligase
Including the following 4 domains:
Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:6.3.4.9)
Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC:6.3.4.10)
Alternative name(s):
Holocarboxylase synthetase
Short name:
HCS
Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11)
Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15)
Gene namesi
Name:BPL1
Synonyms:ACC2
Ordered Locus Names:YDL141W
ORF Names:D2140
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL141w.
SGDiS000002300. BPL1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Biotin--protein ligasePRO_0000064981Add
BLAST

Proteomic databases

MaxQBiP48445.
PaxDbiP48445.
PeptideAtlasiP48445.

Expressioni

Gene expression databases

GenevestigatoriP48445.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi31920. 22 interactions.
IntActiP48445. 1 interaction.
MINTiMINT-2780539.
STRINGi4932.YDL141W.

Structurei

3D structure databases

ProteinModelPortaliP48445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the biotin--protein ligase family.Curated

Phylogenomic databases

eggNOGiCOG0340.
GeneTreeiENSGT00390000002960.
HOGENOMiHOG000214585.
InParanoidiP48445.
KOiK01942.
OMAiAVVYCKV.
OrthoDBiEOG7KQ2BG.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR019197. Biotin-prot_ligase_N.
IPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LipA_LipB.
IPR029062. Class_I_gatase-like.
[Graphical view]
PANTHERiPTHR12835. PTHR12835. 1 hit.
PfamiPF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF09825. BPL_N. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

P48445-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNVLVYNGPG TTPGSVKHAV ESLRDFLEPY YAVSTVNVKV LQTEPWMSKT
60 70 80 90 100
SAVVFPGGAD LPYVQACQPI ISRLKHFVSK QGGVFIGFCA GGYFGTSRVE
110 120 130 140 150
FAQGDPTMEV SGSRDLRFFP GTSRGPAYNG FQYNSEAGAR AVKLNLPDGS
160 170 180 190 200
QFSTYFNGGA VFVDADKFDN VEILATYAEH PDVPSSDSGK GQSENPAAVV
210 220 230 240 250
LCTVGRGKVL LTGPHPEFNV RFMRKSTDKH FLETVVENLK AQEIMRLKFM
260 270 280 290 300
RTVLTKTGLN CNNDFNYVRA PNLTPLFMAS APNKRNYLQE MENNLAHHGM
310 320 330 340 350
HANNVELCSE LNAETDSFQF YRGYRASYDA ASSSLLHKEP DEVPKTVIFP
360 370 380 390 400
GVDEDIPPFQ YTPNFDMKEY FKYLNVQNTI GSLLLYGEVV TSTSTILNNN
410 420 430 440 450
KSLLSSIPES TLLHVGTIQV SGRGRGGNTW INPKGVCAST AVVTMPLQSP
460 470 480 490 500
VTNRNISVVF VQYLSMLAYC KAILSYAPGF SDIPVRIKWP NDLYALSPTY
510 520 530 540 550
YKRKNLKLVN TGFEHTKLPL GDIEPAYLKI SGLLVNTHFI NNKYCLLLGC
560 570 580 590 600
GINLTSDGPT TSLQTWIDIL NEERQQLHLD LLPAIKAEKL QALYMNNLEV
610 620 630 640 650
ILKQFINYGA AEILPSYYEL WLHSNQIVTL PDHGNTQAMI TGITEDYGLL
660 670 680 690
IAKELVSGSS TQFTGNVYNL QPDGNTFDIF KSLIAKKVQS
Length:690
Mass (Da):76,363
Last modified:February 1, 1996 - v1
Checksum:iADA6BFE411C656AB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U27182 Genomic DNA. Translation: AAC49057.1.
X96876 Genomic DNA. Translation: CAA65617.1.
Z74189 Genomic DNA. Translation: CAA98714.1.
BK006938 Genomic DNA. Translation: DAA11717.1.
PIRiS64646.
RefSeqiNP_010140.1. NM_001180201.1.

Genome annotation databases

EnsemblFungiiYDL141W; YDL141W; YDL141W.
GeneIDi851414.
KEGGisce:YDL141W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U27182 Genomic DNA. Translation: AAC49057.1 .
X96876 Genomic DNA. Translation: CAA65617.1 .
Z74189 Genomic DNA. Translation: CAA98714.1 .
BK006938 Genomic DNA. Translation: DAA11717.1 .
PIRi S64646.
RefSeqi NP_010140.1. NM_001180201.1.

3D structure databases

ProteinModelPortali P48445.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31920. 22 interactions.
IntActi P48445. 1 interaction.
MINTi MINT-2780539.
STRINGi 4932.YDL141W.

Proteomic databases

MaxQBi P48445.
PaxDbi P48445.
PeptideAtlasi P48445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL141W ; YDL141W ; YDL141W .
GeneIDi 851414.
KEGGi sce:YDL141W.

Organism-specific databases

CYGDi YDL141w.
SGDi S000002300. BPL1.

Phylogenomic databases

eggNOGi COG0340.
GeneTreei ENSGT00390000002960.
HOGENOMi HOG000214585.
InParanoidi P48445.
KOi K01942.
OMAi AVVYCKV.
OrthoDBi EOG7KQ2BG.

Enzyme and pathway databases

BioCyci YEAST:YDL141W-MONOMER.
Reactomei REACT_188774. Biotin transport and metabolism.

Miscellaneous databases

NextBioi 968603.
PROi P48445.

Gene expression databases

Genevestigatori P48445.

Family and domain databases

Gene3Di 3.40.50.880. 1 hit.
InterProi IPR019197. Biotin-prot_ligase_N.
IPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LipA_LipB.
IPR029062. Class_I_gatase-like.
[Graphical view ]
PANTHERi PTHR12835. PTHR12835. 1 hit.
Pfami PF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF09825. BPL_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae."
    Cronan J.E. Jr., Wallace J.C.
    FEMS Microbiol. Lett. 130:221-230(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
    Woelfl S., Haneman V., Saluz H.P.
    Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBPL1_YEAST
AccessioniPrimary (citable) accession number: P48445
Secondary accession number(s): D6VRK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3