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Protein

Biotin--protein ligase

Gene

BPL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase.

Miscellaneous

Present with 1970 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxytransferase] = AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxytransferase].
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].

GO - Molecular functioni

GO - Biological processi

  • protein biotinylation Source: SGD

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YDL141W-MONOMER.
ReactomeiR-SCE-196780. Biotin transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin--protein ligase (EC:6.3.4.-)
Alternative name(s):
Biotin apo-protein ligase
Including the following 4 domains:
Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:6.3.4.9)
Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC:6.3.4.10)
Alternative name(s):
Holocarboxylase synthetase
Short name:
HCS
Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11)
Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15)
Gene namesi
Name:BPL1
Synonyms:ACC2
Ordered Locus Names:YDL141W
ORF Names:D2140
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL141W.
SGDiS000002300. BPL1.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649811 – 690Biotin--protein ligaseAdd BLAST690

Proteomic databases

MaxQBiP48445.
PRIDEiP48445.

PTM databases

iPTMnetiP48445.

Interactioni

Subunit structurei

Monomer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
RNT1Q025552EBI-3748,EBI-15673

Protein-protein interaction databases

BioGridi31920. 22 interactors.
IntActiP48445. 8 interactors.
MINTiMINT-2780539.
STRINGi4932.YDL141W.

Structurei

3D structure databases

ProteinModelPortaliP48445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini378 – 606BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST229

Sequence similaritiesi

Belongs to the biotin--protein ligase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002960.
HOGENOMiHOG000214585.
InParanoidiP48445.
KOiK01942.
OMAiFFPGTCR.
OrthoDBiEOG092C16UD.

Family and domain databases

CDDicd16442. BPL. 1 hit.
Gene3Di3.40.50.880. 1 hit.
InterProiView protein in InterPro
IPR019197. Biotin-prot_ligase_N.
IPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LPL_catalytic.
IPR029062. Class_I_gatase-like.
PANTHERiPTHR12835:SF10. PTHR12835:SF10. 1 hit.
PfamiView protein in Pfam
PF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF09825. BPL_N. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
PROSITEiView protein in PROSITE
PS51733. BPL_LPL_CATALYTIC. 1 hit.

Sequencei

Sequence statusi: Complete.

P48445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLVYNGPG TTPGSVKHAV ESLRDFLEPY YAVSTVNVKV LQTEPWMSKT
60 70 80 90 100
SAVVFPGGAD LPYVQACQPI ISRLKHFVSK QGGVFIGFCA GGYFGTSRVE
110 120 130 140 150
FAQGDPTMEV SGSRDLRFFP GTSRGPAYNG FQYNSEAGAR AVKLNLPDGS
160 170 180 190 200
QFSTYFNGGA VFVDADKFDN VEILATYAEH PDVPSSDSGK GQSENPAAVV
210 220 230 240 250
LCTVGRGKVL LTGPHPEFNV RFMRKSTDKH FLETVVENLK AQEIMRLKFM
260 270 280 290 300
RTVLTKTGLN CNNDFNYVRA PNLTPLFMAS APNKRNYLQE MENNLAHHGM
310 320 330 340 350
HANNVELCSE LNAETDSFQF YRGYRASYDA ASSSLLHKEP DEVPKTVIFP
360 370 380 390 400
GVDEDIPPFQ YTPNFDMKEY FKYLNVQNTI GSLLLYGEVV TSTSTILNNN
410 420 430 440 450
KSLLSSIPES TLLHVGTIQV SGRGRGGNTW INPKGVCAST AVVTMPLQSP
460 470 480 490 500
VTNRNISVVF VQYLSMLAYC KAILSYAPGF SDIPVRIKWP NDLYALSPTY
510 520 530 540 550
YKRKNLKLVN TGFEHTKLPL GDIEPAYLKI SGLLVNTHFI NNKYCLLLGC
560 570 580 590 600
GINLTSDGPT TSLQTWIDIL NEERQQLHLD LLPAIKAEKL QALYMNNLEV
610 620 630 640 650
ILKQFINYGA AEILPSYYEL WLHSNQIVTL PDHGNTQAMI TGITEDYGLL
660 670 680 690
IAKELVSGSS TQFTGNVYNL QPDGNTFDIF KSLIAKKVQS
Length:690
Mass (Da):76,363
Last modified:February 1, 1996 - v1
Checksum:iADA6BFE411C656AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27182 Genomic DNA. Translation: AAC49057.1.
X96876 Genomic DNA. Translation: CAA65617.1.
Z74189 Genomic DNA. Translation: CAA98714.1.
BK006938 Genomic DNA. Translation: DAA11717.1.
PIRiS64646.
RefSeqiNP_010140.1. NM_001180201.1.

Genome annotation databases

EnsemblFungiiYDL141W; YDL141W; YDL141W.
GeneIDi851414.
KEGGisce:YDL141W.

Similar proteinsi

Entry informationi

Entry nameiBPL1_YEAST
AccessioniPrimary (citable) accession number: P48445
Secondary accession number(s): D6VRK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 27, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names