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P48445

- BPL1_YEAST

UniProt

P48445 - BPL1_YEAST

Protein

Biotin--protein ligase

Gene

BPL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase.

    Catalytic activityi

    ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxytransferase] = AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxytransferase].
    ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)].
    ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)].
    ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. biotin-[acetyl-CoA-carboxylase] ligase activity Source: SGD
    3. biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity Source: UniProtKB-EC
    4. biotin-[methylmalonyl-CoA-carboxytransferase] ligase activity Source: UniProtKB-EC
    5. biotin-[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase activity Source: UniProtKB-EC
    6. biotin-[pyruvate-carboxylase] ligase activity Source: SGD

    GO - Biological processi

    1. protein biotinylation Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YDL141W-MONOMER.
    ReactomeiREACT_188774. Biotin transport and metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin--protein ligase (EC:6.3.4.-)
    Alternative name(s):
    Biotin apo-protein ligase
    Including the following 4 domains:
    Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:6.3.4.9)
    Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC:6.3.4.10)
    Alternative name(s):
    Holocarboxylase synthetase
    Short name:
    HCS
    Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11)
    Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15)
    Gene namesi
    Name:BPL1
    Synonyms:ACC2
    Ordered Locus Names:YDL141W
    ORF Names:D2140
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL141w.
    SGDiS000002300. BPL1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 690690Biotin--protein ligasePRO_0000064981Add
    BLAST

    Proteomic databases

    MaxQBiP48445.
    PaxDbiP48445.
    PeptideAtlasiP48445.

    Expressioni

    Gene expression databases

    GenevestigatoriP48445.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi31920. 21 interactions.
    IntActiP48445. 1 interaction.
    MINTiMINT-2780539.
    STRINGi4932.YDL141W.

    Structurei

    3D structure databases

    ProteinModelPortaliP48445.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the biotin--protein ligase family.Curated

    Phylogenomic databases

    eggNOGiCOG0340.
    GeneTreeiENSGT00390000002960.
    HOGENOMiHOG000214585.
    KOiK01942.
    OMAiAVVYCKV.
    OrthoDBiEOG7KQ2BG.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR019197. Biotin-prot_ligase_N.
    IPR004408. Biotin_CoA_COase_ligase.
    IPR003142. BPL_C.
    IPR004143. BPL_LipA_LipB.
    IPR029062. Class_I_gatase-like.
    [Graphical view]
    PANTHERiPTHR12835. PTHR12835. 1 hit.
    PfamiPF02237. BPL_C. 1 hit.
    PF03099. BPL_LplA_LipB. 1 hit.
    PF09825. BPL_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48445-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNVLVYNGPG TTPGSVKHAV ESLRDFLEPY YAVSTVNVKV LQTEPWMSKT    50
    SAVVFPGGAD LPYVQACQPI ISRLKHFVSK QGGVFIGFCA GGYFGTSRVE 100
    FAQGDPTMEV SGSRDLRFFP GTSRGPAYNG FQYNSEAGAR AVKLNLPDGS 150
    QFSTYFNGGA VFVDADKFDN VEILATYAEH PDVPSSDSGK GQSENPAAVV 200
    LCTVGRGKVL LTGPHPEFNV RFMRKSTDKH FLETVVENLK AQEIMRLKFM 250
    RTVLTKTGLN CNNDFNYVRA PNLTPLFMAS APNKRNYLQE MENNLAHHGM 300
    HANNVELCSE LNAETDSFQF YRGYRASYDA ASSSLLHKEP DEVPKTVIFP 350
    GVDEDIPPFQ YTPNFDMKEY FKYLNVQNTI GSLLLYGEVV TSTSTILNNN 400
    KSLLSSIPES TLLHVGTIQV SGRGRGGNTW INPKGVCAST AVVTMPLQSP 450
    VTNRNISVVF VQYLSMLAYC KAILSYAPGF SDIPVRIKWP NDLYALSPTY 500
    YKRKNLKLVN TGFEHTKLPL GDIEPAYLKI SGLLVNTHFI NNKYCLLLGC 550
    GINLTSDGPT TSLQTWIDIL NEERQQLHLD LLPAIKAEKL QALYMNNLEV 600
    ILKQFINYGA AEILPSYYEL WLHSNQIVTL PDHGNTQAMI TGITEDYGLL 650
    IAKELVSGSS TQFTGNVYNL QPDGNTFDIF KSLIAKKVQS 690
    Length:690
    Mass (Da):76,363
    Last modified:February 1, 1996 - v1
    Checksum:iADA6BFE411C656AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27182 Genomic DNA. Translation: AAC49057.1.
    X96876 Genomic DNA. Translation: CAA65617.1.
    Z74189 Genomic DNA. Translation: CAA98714.1.
    BK006938 Genomic DNA. Translation: DAA11717.1.
    PIRiS64646.
    RefSeqiNP_010140.1. NM_001180201.1.

    Genome annotation databases

    EnsemblFungiiYDL141W; YDL141W; YDL141W.
    GeneIDi851414.
    KEGGisce:YDL141W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27182 Genomic DNA. Translation: AAC49057.1 .
    X96876 Genomic DNA. Translation: CAA65617.1 .
    Z74189 Genomic DNA. Translation: CAA98714.1 .
    BK006938 Genomic DNA. Translation: DAA11717.1 .
    PIRi S64646.
    RefSeqi NP_010140.1. NM_001180201.1.

    3D structure databases

    ProteinModelPortali P48445.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31920. 21 interactions.
    IntActi P48445. 1 interaction.
    MINTi MINT-2780539.
    STRINGi 4932.YDL141W.

    Proteomic databases

    MaxQBi P48445.
    PaxDbi P48445.
    PeptideAtlasi P48445.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL141W ; YDL141W ; YDL141W .
    GeneIDi 851414.
    KEGGi sce:YDL141W.

    Organism-specific databases

    CYGDi YDL141w.
    SGDi S000002300. BPL1.

    Phylogenomic databases

    eggNOGi COG0340.
    GeneTreei ENSGT00390000002960.
    HOGENOMi HOG000214585.
    KOi K01942.
    OMAi AVVYCKV.
    OrthoDBi EOG7KQ2BG.

    Enzyme and pathway databases

    BioCyci YEAST:YDL141W-MONOMER.
    Reactomei REACT_188774. Biotin transport and metabolism.

    Miscellaneous databases

    NextBioi 968603.
    PROi P48445.

    Gene expression databases

    Genevestigatori P48445.

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    InterProi IPR019197. Biotin-prot_ligase_N.
    IPR004408. Biotin_CoA_COase_ligase.
    IPR003142. BPL_C.
    IPR004143. BPL_LipA_LipB.
    IPR029062. Class_I_gatase-like.
    [Graphical view ]
    PANTHERi PTHR12835. PTHR12835. 1 hit.
    Pfami PF02237. BPL_C. 1 hit.
    PF03099. BPL_LplA_LipB. 1 hit.
    PF09825. BPL_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae."
      Cronan J.E. Jr., Wallace J.C.
      FEMS Microbiol. Lett. 130:221-230(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
      Woelfl S., Haneman V., Saluz H.P.
      Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBPL1_YEAST
    AccessioniPrimary (citable) accession number: P48445
    Secondary accession number(s): D6VRK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1970 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3