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P48441

- IDUA_MOUSE

UniProt

P48441 - IDUA_MOUSE

Protein

Alpha-L-iduronidase

Gene

Idua

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei171 – 1711SubstrateBy similarity
    Active sitei172 – 1721Proton donorPROSITE-ProRule annotation
    Binding sitei254 – 2541SubstrateBy similarity
    Active sitei289 – 2891NucleophileBy similarity
    Binding sitei339 – 3391SubstrateBy similarity
    Binding sitei353 – 3531SubstrateBy similarity

    GO - Molecular functioni

    1. L-iduronidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell morphogenesis Source: MGI
    3. chemical homeostasis Source: MGI
    4. dermatan sulfate catabolic process Source: UniProtKB
    5. limb morphogenesis Source: MGI
    6. lysosome organization Source: MGI
    7. skeletal system morphogenesis Source: MGI

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_198967. HS-GAG degradation.
    REACT_198981. CS/DS degradation.

    Protein family/group databases

    CAZyiGH39. Glycoside Hydrolase Family 39.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-iduronidase (EC:3.2.1.76)
    Gene namesi
    Name:Idua
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96418. Idua.

    Subcellular locationi

    Lysosome By similarity

    GO - Cellular componenti

    1. coated vesicle Source: Ensembl
    2. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 634618Alpha-L-iduronidasePRO_0000012201Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi531 ↔ 567By similarity

    Post-translational modificationi

    N-glycosylation contributes to substrate binding and is required for full enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP48441.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    ArrayExpressiP48441.
    BgeeiP48441.
    CleanExiMM_IDUA.
    GenevestigatoriP48441.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP48441.
    SMRiP48441. Positions 17-632.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni295 – 2962Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 39 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3664.
    HOGENOMiHOG000007042.
    HOVERGENiHBG006121.
    InParanoidiP48441.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR003961. Fibronectin_type3.
    IPR000514. Glyco_hydro_39.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01229. Glyco_hydro_39. 1 hit.
    [Graphical view]
    PRINTSiPR00745. GLHYDRLASE39.
    SUPFAMiSSF49265. SSF49265. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01027. GLYCOSYL_HYDROL_F39. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48441-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTFFAAFLA APLALAESPY LVRVDAARPL RPLLPFWRST GFCPPLPHDQ    50
    ADQYDLSWDQ QLNLAYIGAV PHSGIEQVRI HWLLDLITAR KSPGQGLMYN 100
    FTHLDAFLDL LMENQLLPGF ELMGSPSGYF TDFDDKQQVF EWKDLVSLLA 150
    RRYIGRYGLT HVSKWNFETW NEPDHHDFDN VSMTTQGFLN YYDACSEGLR 200
    IASPTLKLGG PGDSFHPLPR SPMCWSLLGH CANGTNFFTG EVGVRLDYIS 250
    LHKKGAGSSI AILEQEMAVV EQVQQLFPEF KDTPIYNDEA DPLVGWSLPQ 300
    PWRADVTYAA LVVKVIAQHQ NLLFANSSSS MRYVLLSNDN AFLSYHPYPF 350
    SQRTLTARFQ VNNTHPPHVQ LLRKPVLTVM GLMALLDGEQ LWAEVSKAGA 400
    VLDSNHTVGV LASTHHPEGS AAAWSTTVLI YTSDDTHAHP NHSIPVTLRL 450
    RGVPPGLDLV YIVLYLDNQL SSPYSAWQHM GQPVFPSAEQ FRRMRMVEDP 500
    VAEAPRPFPA RGRLTLHRKL PVPSLLLVHV CTRPLKPPGQ VSRLRALPLT 550
    HGQLILVWSD ERVGSKCLWT YEIQFSQKGE EYAPINRRPS TFNLFVFSPD 600
    TAVVSGSYRV RALDYWARPG PFSDPVTYLD VPAS 634
    Length:634
    Mass (Da):71,254
    Last modified:July 27, 2011 - v2
    Checksum:i41A8F5B036B360DB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti359 – 3591F → S in AAC42044. (PubMed:7698753)Curated
    Sequence conflicti423 – 4231A → G in AAC42044. (PubMed:7698753)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34111 mRNA. Translation: AAC42044.1.
    AC161813 Genomic DNA. No translation available.
    PIRiA55683.
    UniGeneiMm.358608.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34111 mRNA. Translation: AAC42044.1 .
    AC161813 Genomic DNA. No translation available.
    PIRi A55683.
    UniGenei Mm.358608.

    3D structure databases

    ProteinModelPortali P48441.
    SMRi P48441. Positions 17-632.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH39. Glycoside Hydrolase Family 39.

    Proteomic databases

    PRIDEi P48441.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:96418. Idua.

    Phylogenomic databases

    eggNOGi COG3664.
    HOGENOMi HOG000007042.
    HOVERGENi HBG006121.
    InParanoidi P48441.

    Enzyme and pathway databases

    Reactomei REACT_198967. HS-GAG degradation.
    REACT_198981. CS/DS degradation.

    Miscellaneous databases

    ChiTaRSi IDUA. mouse.
    PROi P48441.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48441.
    Bgeei P48441.
    CleanExi MM_IDUA.
    Genevestigatori P48441.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR003961. Fibronectin_type3.
    IPR000514. Glyco_hydro_39.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01229. Glyco_hydro_39. 1 hit.
    [Graphical view ]
    PRINTSi PR00745. GLHYDRLASE39.
    SUPFAMi SSF49265. SSF49265. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01027. GLYCOSYL_HYDROL_F39. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine alpha-L-iduronidase: cDNA isolation and expression."
      Clarke L.A.
      Genomics 24:311-316(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
      Strain: C57BL/6 X CBA.
      Tissue: Liver.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiIDUA_MOUSE
    AccessioniPrimary (citable) accession number: P48441
    Secondary accession number(s): E9QM17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3