Reviewed, UniProtKB/Swiss-Prot P48440 (OST48_CHICK)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
      Short name=Oligosaccharyl transferase 48 kDa subunit
      Short name=DDOST 48 kDa subunit
    EC=2.4.1.119
Alternative name(s):
    OST 50 kDa subunit

Gene names

Name:	DDOST
Synonyms:	OST48

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000058094
Regions
	Topological domain	1 – 383	383	Lumenal Potential
	Transmembrane	384 – 404	21	Potential
	Topological domain	405 – 413	9	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P48440-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 5CDC51930F5D4BF1	FASTA	413	45,912
	10 20 30 40 50 60 GPRSLVLLEN LNLRDTHSLF FRSLADRGFE LTFRTADDAG LSLIKYGEFL YDNLIIFSPS 70 80 90 100 110 120 IEDFGGNINV ETITAFIDGG GSVLVAASSD IGDPLRELGS ECGIEFDEER TAVIDHHNYD 130 140 150 160 170 180 ISDPGQHTLI VADAENLLKA PTIVGKKALN PILFRGVGMV ADPDNPLVLD ILTGSSTSYS 190 200 210 220 230 240 FFPDKPITQY PHAVGKNTLL IAGLQARNNA RVVFSGSLDF FSDAFFSSAV QKAAPGSKRY 250 260 270 280 290 300 SQTGNYELAV ALSRWVFKEE GVLRVGAVSH HRVGELAPPN AYTVTDLVEY SIVIEKLSDG 310 320 330 340 350 360 KWIPFDGDDI QLEFVRIDPF VRTFLKRNGG KYSVQFKLPD VYGVFQFKVD YNRLGYTHLY 370 380 390 400 410 SSTQVSVRPL QHTQYERFIP SAYPYYAGAF SMMVGLFMFS IVFLHMKEKE KSD

References

[1]	"Purification and characterization of avian oligosaccharyltransferase. Complete amino acid sequence of the 50-kDa subunit." Kumar V., Heinemann F.S., Ozols J. J. Biol. Chem. 269:13451-13457(1994) [PubMed: 8175777] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Oviduct.

Cross-references

Sequence databases
IPI	IPI00822865.
PIR	A54127.
UniGene	Gga.13565
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P48440.
Genome annotation databases
Ensembl	ENSGALT00000016373; ENSGALP00000016354; ENSGALG00000010073; Gallus gallus. [Genome view] ENSGALT00000039102; ENSGALP00000038313; ENSGALG00000010073; Gallus gallus. [Genome view]
Phylogenomic databases
eggNOG	veNOG07573.
HOGENOM	HBG317312.
HOVERGEN	P48440.
InParanoid	P48440.
PhylomeDB	P48440.
Enzyme and pathway databases
BRENDA	2.4.1.119. 4.
Family and domain databases
InterPro	IPR005013. DDOST_48kDa. [Graphical view]
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_CHICK

Accession

Primary (citable) accession number: P48440

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 19, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)