Reviewed, UniProtKB/Swiss-Prot P48439 (OST3_YEAST)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3
    EC=2.4.1.119
Alternative name(s):
    Oligosaccharyl transferase subunit OST3
    Oligosaccharyl transferase subunit gamma
    Oligosaccharyl transferase 34 kDa subunit

Gene names

Name:	OST3
Ordered Locus Names:	YOR085W
ORF Names:	YOR3124W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyl transferase (OST) complex, which apears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST3, OST4 and STT3 probably form a subcomplex. May interact directly with OST1, OST2, OST4 and STT3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Miscellaneous	Present with 4030 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the OST3/OST6 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Ref.7 Inferred from physical interaction. Source: SGD protein complex assembly Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Ref.1 Inferred from mutant phenotype. Source: SGD oligosaccharyltransferase complex Ref.2 Ref.7 Ref.11 Inferred from physical interaction. Source: SGD
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.1 Ref.7 Inferred from mutant phenotype. Source: SGD protein binding Ref.9 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
OST4	Q99380	1	EBI-12680,EBI-12689
SBH1	P52870	1	EBI-12680,EBI-16410
STT3	P39007	1	EBI-12680,EBI-18447

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 350	328	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3		PRO_0000020275
Regions
	Transmembrane	186 – 203	18	Potential
	Transmembrane	218 – 237	20	Potential
	Transmembrane	272 – 288	17	Potential
	Transmembrane	310 – 329	20	Potential
	Topological domain	330 – 350	21	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P48439-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: EEA573BC14E99380	FASTA	350	39,483
	10 20 30 40 50 60 MNWLFLVSLV FFCGVSTHPA LAMSSNRLLK LANKSPKKII PLKDSSFENI LAPPHENAYI 70 80 90 100 110 120 VALFTATAPE IGCSLCLELE SEYDTIVASW FDDHPDAKSS NSDTSIFFTK VNLEDPSKTI 130 140 150 160 170 180 PKAFQFFQLN NVPRLFIFKP NSPSILDHSV ISISTDTGSE RMKQIIQAIK QFSQVNDFSL 190 200 210 220 230 240 HLPMDWTPII TSTIITFITV LLFKKQSKLM FSIISSRIIW ATLSTFFIIC MISAYMFNQI 250 260 270 280 290 300 RNTQLAGVGP KGEVMYFLPN EFQHQFAIET QVMVLIYGTL AALVVVLVKG IQFLRSHLYP 310 320 330 340 350 ETKKAYFIDA ILASFCALFI YVFFAALTTV FTIKSPAYPF PLLRLSAPFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional characterization of Ost3p. Loss of the 34-kD subunit of the Saccharomyces cerevisiae oligosaccharyltransferase results in biased underglycosylation of acceptor substrates." Karaoglu D., Kelleher D.J., Gilmore R. J. Cell Biol. 130:567-577(1995) [PubMed: 7622558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-37; 99-103; 123-153 AND 345-350.
[2]	"The oligosaccharyltransferase complex from Saccharomyces cerevisiae. Isolation of the OST6 gene, its synthetic interaction with OST3, and analysis of the native complex." Knauer R., Lehle L. J. Biol. Chem. 274:17249-17256(1999) [PubMed: 10358084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides." Kelleher D.J., Gilmore R. J. Biol. Chem. 269:12908-12917(1994) [PubMed: 8175708] [Abstract] Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[7]	"The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p." Karaoglu D., Kelleher D.J., Gilmore R. J. Biol. Chem. 272:32513-32520(1997) [PubMed: 9405463] [Abstract] Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX, INTERACTION WITH OST4 AND STT3.
[8]	"The oligosaccharyltransferase complex from yeast." Knauer R., Lehle L. Biochim. Biophys. Acta 1426:259-273(1999) [PubMed: 9878773] [Abstract] Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[9]	"Studies on the role of the hydrophobic domain of Ost4p in interactions with other subunits of yeast oligosaccharyl transferase." Kim H., Park H., Montalvo L., Lennarz W.J. Proc. Natl. Acad. Sci. U.S.A. 97:1516-1520(2000) [PubMed: 10677492] [Abstract] Cited for: INTERACTION WITH OST4 AND STT3.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit." Schwarz M., Knauer R., Lehle L. FEBS Lett. 579:6564-6568(2005) [PubMed: 16297388] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[12]	"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast." Spirig U., Bodmer D., Wacker M., Burda P., Aebi M. Glycobiology 15:1396-1406(2005) [PubMed: 16096346] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[13]	"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons." Yan A., Lennarz W.J. Glycobiology 15:1407-1415(2005) [PubMed: 16096345] [Abstract] Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[14]	"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions." Yan A., Wu E., Lennarz W.J. Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed: 15886282] [Abstract] Cited for: TOPOLOGY, INTERACTION WITH OST1; OST2; OST4 AND STT3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U25052 Genomic DNA. Translation: AAC49042.1. X79596 Genomic DNA. Translation: CAA56108.1. X94335 Genomic DNA. Translation: CAA64007.1. Z74993 Genomic DNA. Translation: CAA99280.1. AY558041 Genomic DNA. Translation: AAS56367.1.
PIR	S61646.
RefSeq	NP_014728.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2456N.
IntAct	P48439. 9 interactions.
STRING	P48439.
Genome annotation databases
Ensembl	YOR085W; YOR085W; YOR085W; Saccharomyces cerevisiae. [Genome view]
GeneID	854252.
GenomeReviews	Gene locus YOR085W in contig Y13140_GR.
KEGG	sce:YOR085W.
NMPDR	fig|4932.3.peg.5831.
Organism-specific databases
CYGD	YOR085w.
SGD	S000005611. OST3.
Phylogenomic databases
HOGENOM	P48439.
OMA	QLMDWTK.
Enzyme and pathway databases
BRENDA	2.4.1.119. 250.
Gene expression databases
ArrayExpress	P48439.
Genevestigator	P48439.
GermOnline	YOR085W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006844. OST3_OST6. [Graphical view]
PANTHER	PTHR12692. OST3_OST6. 1 hit.
Pfam	PF04756. OST3_OST6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976171.

Entry information

Entry name

OST3_YEAST

Accession

Primary (citable) accession number: P48439

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)