P48438 (GSTF_BRAOT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase EC=2.5.1.18 Alternative name(s): GST class-phi
Organism	Brassica oleracea var. italica (Broccoli)
Taxonomic identifier	36774 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica › Brassica oleracea

Protein attributes

Sequence length	76 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›76	›76	Glutathione S-transferase		PRO_0000185840
Regions
	Domain	‹1 – 40	›40	GST N-terminal
	Domain	41 – ›76	›36	GST C-terminal
Experimental info
	Non-adjacent residues	40 – 41	2
	Non-terminal residue	1	1
	Non-terminal residue	76	1

Sequences

	Sequence		Length	Mass (Da)
	P48438 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F62C58C6AD3543E7	FASTA	76	8,268
	10 20 30 40 50 60 XVAFETVPVD LMKGEHKQPA YLALQPFGTV PAVVDGDYXL LSAVLDVYEA HLHGYLAGDF 70 VSLADLAHLP FTDYLV

References

[1]

"A glutathione S-transferase (GST) isozyme from broccoli with significant sequence homology to the mammalian theta-class of GSTs." Lopez M.F., Patton W.F., Sawlivich W.B., Erdjument-Bromage H., Barry P., Gmyrek K., Hines T., Tempst P., Skea W.M. Biochim. Biophys. Acta 1205:29-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	S43401.
3D structure databases
ProteinModelPortal	P48438.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF_BRAOT

Accession

Primary (citable) accession number: P48438

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program