Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prospero homeobox protein 1

Gene

Prox1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.1 Publication1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi573 – 63563Prospero-type homeoboxAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • acinar cell differentiation Source: MGI
  • aorta smooth muscle tissue morphogenesis Source: BHF-UCL
  • atrial cardiac muscle tissue morphogenesis Source: BHF-UCL
  • branching involved in pancreas morphogenesis Source: MGI
  • cell fate determination Source: MGI
  • cerebellar granule cell differentiation Source: BHF-UCL
  • circadian rhythm Source: UniProtKB
  • dentate gyrus development Source: BHF-UCL
  • embryonic retina morphogenesis in camera-type eye Source: BHF-UCL
  • endocardium formation Source: BHF-UCL
  • endothelial cell differentiation Source: MGI
  • hepatocyte cell migration Source: MGI
  • hepatocyte differentiation Source: BHF-UCL
  • hepatocyte proliferation Source: MGI
  • inner ear development Source: MGI
  • kidney development Source: Ensembl
  • lens fiber cell morphogenesis Source: BHF-UCL
  • lens morphogenesis in camera-type eye Source: BHF-UCL
  • lung development Source: Ensembl
  • lymphangiogenesis Source: MGI
  • lymphatic endothelial cell differentiation Source: MGI
  • lymph vessel development Source: MGI
  • negative regulation of bile acid biosynthetic process Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of viral genome replication Source: MGI
  • neuronal stem cell population maintenance Source: BHF-UCL
  • optic placode formation involved in camera-type eye formation Source: BHF-UCL
  • positive regulation of cell cycle Source: BHF-UCL
  • positive regulation of cell cycle checkpoint Source: MGI
  • positive regulation of cell proliferation Source: BHF-UCL
  • positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  • positive regulation of endothelial cell migration Source: MGI
  • positive regulation of endothelial cell proliferation Source: MGI
  • positive regulation of forebrain neuron differentiation Source: BHF-UCL
  • positive regulation of heart growth Source: BHF-UCL
  • positive regulation of neural precursor cell proliferation Source: BHF-UCL
  • positive regulation of sarcomere organization Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of gene expression Source: BHF-UCL
  • regulation of transcription involved in lymphatic endothelial cell fate commitment Source: MGI
  • response to nutrient levels Source: Ensembl
  • skeletal muscle thin filament assembly Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
  • venous blood vessel morphogenesis Source: BHF-UCL
  • ventricular cardiac muscle tissue morphogenesis Source: BHF-UCL
  • ventricular cardiac myofibril assembly Source: BHF-UCL
  • ventricular septum morphogenesis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Prospero homeobox protein 1
Alternative name(s):
Homeobox prospero-like protein PROX1
Short name:
PROX-1
Gene namesi
Name:Prox1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97772. Prox1.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: RORG promotes its nuclear localization.1 Publication

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 737737Prospero homeobox protein 1PRO_0000208881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei177 – 1771PhosphoserineBy similarity
Modified residuei179 – 1791PhosphoserineCombined sources
Modified residuei199 – 1991PhosphoserineBy similarity
Modified residuei291 – 2911PhosphoserineCombined sources
Modified residuei295 – 2951PhosphoserineCombined sources
Cross-linki324 – 324Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei511 – 5111PhosphoserineCombined sources
Modified residuei514 – 5141PhosphoserineCombined sources
Modified residuei557 – 5571PhosphoserineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP48437.
PaxDbiP48437.
PeptideAtlasiP48437.
PRIDEiP48437.

PTM databases

iPTMnetiP48437.
PhosphoSiteiP48437.

Expressioni

Tissue specificityi

Expressed in the young neurons of the subventricular region of the CNS, developing eye lens and pancreas. It is also found in the developing liver, heart and skeletal muscle. In the eye, expressed in the lens and retina at postnatal day 10. In the retina, localized to the inner nuclear layer. In the lens, localized to epithelial and fiber cells.2 Publications

Inductioni

Expressed in a circadian manner in the liver with a peak at ZT4.1 Publication

Gene expression databases

BgeeiENSMUSG00000010175.
CleanExiMM_PROX1.
GenevisibleiP48437. MM.

Interactioni

Subunit structurei

Interacts with RORA and RORG (via AF-2 motif).1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202396. 2 interactions.
DIPiDIP-58960N.
IntActiP48437. 1 interaction.
MINTiMINT-4108644.
STRINGi10090.ENSMUSP00000010319.

Structurei

3D structure databases

ProteinModelPortaliP48437.
SMRiP48437. Positions 575-737.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2828Interaction with RORG1 PublicationAdd
BLAST
Regioni636 – 737102Prospero-likeAdd
BLAST
Regioni723 – 7297Essential for nuclear localization, interaction with RORG, repression of RORG transcriptional activator activity1 Publication

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi215 – 2195Poly-Gln

Domaini

The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.1 Publication

Sequence similaritiesi

Belongs to the Prospero homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3779. Eukaryota.
ENOG410ZE21. LUCA.
GeneTreeiENSGT00530000063507.
HOGENOMiHOG000115708.
HOVERGENiHBG053693.
InParanoidiP48437.
KOiK20211.
OMAiSCFMSRN.
OrthoDBiEOG091G02KS.
PhylomeDBiP48437.
TreeFamiTF316638.

Family and domain databases

Gene3Di1.10.10.500. 1 hit.
InterProiIPR023082. Homeo_prospero_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF05044. HPD. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.

Sequencei

Sequence statusi: Complete.

P48437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDHDSTALL SRQTKRRRVD IGVKRTVGTA SAFFAKARAT FFSAMNPQGS
60 70 80 90 100
EQDVEYSVVQ HADGEKSNVL RKLLKRANSY EDAMMPFPGA TIISQLLKNN
110 120 130 140 150
MNKNGGTEPS FQASGLSSTG SEVHQEDICS NSSRDSPPEC LSPFGRPTMS
160 170 180 190 200
QFDVDRLCDE HLRAKRARVE NIIRGMSHSP SVALRGNENE REMAPQSVSP
210 220 230 240 250
RESYRENKRK QKLPQQQQQS FQQLVSARKE QKREERRQLK QQLEDMQKQL
260 270 280 290 300
RQLQEKFYQV YDSTDSENDE DGDLSEDSMR SEILDARAQD SVGRSDNEMC
310 320 330 340 350
ELDPGQFIDR ARALIREQEM AENKPKREGS NKERDHGPNS LQPEGKHLAE
360 370 380 390 400
TLKQELNTAM SQVVDTVVKV FSAKPSRQVP QVFPPLQIPQ ARFAVNGENH
410 420 430 440 450
NFHTANQRLQ CFGDVIIPNP LDTFGSVQMP SSTDQTEALP LVVRKNSSEQ
460 470 480 490 500
SASGPATGGH HQPLHQSPLS ATAGFTTPSF RHPFPLPLMA YPFQSPLGAP
510 520 530 540 550
SGSFSGKDRA SPESLDLTRD TTSLRTKMSS HHLSHHPCSP AHPPSTAEGL
560 570 580 590 600
SLSLIKSECG DLQDMSDISP YSGSAMQEGL SPNHLKKAKL MFFYTRYPSS
610 620 630 640 650
NMLKTYFSDV KFNRCITSQL IKWFSNFREF YYIQMEKYAR QAINDGVTST
660 670 680 690 700
EELSITRDCE LYRALNMHYN KANDFEVPER FLEVAQITLR EFFNAIIAGK
710 720 730
DVDPSWKKAI YKVICKLDSE VPEIFKSPNC LQELLHE
Length:737
Mass (Da):83,126
Last modified:April 27, 2001 - v2
Checksum:i834DF6963FF5C9B5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti685 – 6851A → V in BAC35190 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061576 mRNA. Translation: AAC32824.1.
AK052889 mRNA. Translation: BAC35190.1.
AK143179 mRNA. Translation: BAE25293.1.
BC051411 mRNA. Translation: AAH51411.1.
CCDSiCCDS35822.1.
PIRiJE0269.
RefSeqiNP_032963.1. NM_008937.2.
XP_006497191.1. XM_006497128.2.
UniGeneiMm.132579.
Mm.392678.

Genome annotation databases

EnsembliENSMUST00000010319; ENSMUSP00000010319; ENSMUSG00000010175.
ENSMUST00000175916; ENSMUSP00000135703; ENSMUSG00000010175.
ENSMUST00000177288; ENSMUSP00000135066; ENSMUSG00000010175.
GeneIDi19130.
KEGGimmu:19130.
UCSCiuc007ebc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061576 mRNA. Translation: AAC32824.1.
AK052889 mRNA. Translation: BAC35190.1.
AK143179 mRNA. Translation: BAE25293.1.
BC051411 mRNA. Translation: AAH51411.1.
CCDSiCCDS35822.1.
PIRiJE0269.
RefSeqiNP_032963.1. NM_008937.2.
XP_006497191.1. XM_006497128.2.
UniGeneiMm.132579.
Mm.392678.

3D structure databases

ProteinModelPortaliP48437.
SMRiP48437. Positions 575-737.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202396. 2 interactions.
DIPiDIP-58960N.
IntActiP48437. 1 interaction.
MINTiMINT-4108644.
STRINGi10090.ENSMUSP00000010319.

PTM databases

iPTMnetiP48437.
PhosphoSiteiP48437.

Proteomic databases

MaxQBiP48437.
PaxDbiP48437.
PeptideAtlasiP48437.
PRIDEiP48437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010319; ENSMUSP00000010319; ENSMUSG00000010175.
ENSMUST00000175916; ENSMUSP00000135703; ENSMUSG00000010175.
ENSMUST00000177288; ENSMUSP00000135066; ENSMUSG00000010175.
GeneIDi19130.
KEGGimmu:19130.
UCSCiuc007ebc.2. mouse.

Organism-specific databases

CTDi5629.
MGIiMGI:97772. Prox1.

Phylogenomic databases

eggNOGiKOG3779. Eukaryota.
ENOG410ZE21. LUCA.
GeneTreeiENSGT00530000063507.
HOGENOMiHOG000115708.
HOVERGENiHBG053693.
InParanoidiP48437.
KOiK20211.
OMAiSCFMSRN.
OrthoDBiEOG091G02KS.
PhylomeDBiP48437.
TreeFamiTF316638.

Miscellaneous databases

PROiP48437.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010175.
CleanExiMM_PROX1.
GenevisibleiP48437. MM.

Family and domain databases

Gene3Di1.10.10.500. 1 hit.
InterProiIPR023082. Homeo_prospero_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF05044. HPD. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPROX1_MOUSE
AccessioniPrimary (citable) accession number: P48437
Secondary accession number(s): O88478, Q3UPV1, Q543D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.