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Protein

Prospero homeobox protein 1

Gene

Prox1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.1 Publication1 Publication

GO - Molecular functioni

GO - Biological processi

  • acinar cell differentiation Source: MGI
  • aorta smooth muscle tissue morphogenesis Source: BHF-UCL
  • atrial cardiac muscle tissue morphogenesis Source: BHF-UCL
  • branching involved in pancreas morphogenesis Source: MGI
  • cell fate determination Source: MGI
  • cerebellar granule cell differentiation Source: BHF-UCL
  • circadian rhythm Source: UniProtKB
  • dentate gyrus development Source: BHF-UCL
  • embryonic retina morphogenesis in camera-type eye Source: BHF-UCL
  • endocardium formation Source: BHF-UCL
  • endothelial cell differentiation Source: MGI
  • hepatocyte cell migration Source: MGI
  • hepatocyte differentiation Source: BHF-UCL
  • hepatocyte proliferation Source: MGI
  • inner ear development Source: MGI
  • kidney development Source: Ensembl
  • lens fiber cell morphogenesis Source: BHF-UCL
  • lens morphogenesis in camera-type eye Source: BHF-UCL
  • lung development Source: Ensembl
  • lymphangiogenesis Source: MGI
  • lymphatic endothelial cell differentiation Source: MGI
  • lymph vessel development Source: MGI
  • negative regulation of bile acid biosynthetic process Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • negative regulation of viral genome replication Source: MGI
  • neuronal stem cell population maintenance Source: BHF-UCL
  • optic placode formation involved in camera-type eye formation Source: BHF-UCL
  • positive regulation of cell cycle Source: BHF-UCL
  • positive regulation of cell cycle checkpoint Source: MGI
  • positive regulation of cell proliferation Source: BHF-UCL
  • positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  • positive regulation of endothelial cell migration Source: MGI
  • positive regulation of endothelial cell proliferation Source: MGI
  • positive regulation of forebrain neuron differentiation Source: BHF-UCL
  • positive regulation of heart growth Source: BHF-UCL
  • positive regulation of neural precursor cell proliferation Source: BHF-UCL
  • positive regulation of sarcomere organization Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of gene expression Source: BHF-UCL
  • regulation of transcription involved in lymphatic endothelial cell fate commitment Source: MGI
  • response to nutrient levels Source: Ensembl
  • skeletal muscle thin filament assembly Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
  • venous blood vessel morphogenesis Source: BHF-UCL
  • ventricular cardiac muscle tissue morphogenesis Source: BHF-UCL
  • ventricular cardiac myofibril assembly Source: BHF-UCL
  • ventricular septum morphogenesis Source: BHF-UCL

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Prospero homeobox protein 1
Alternative name(s):
Homeobox prospero-like protein PROX1
Short name:
PROX-1
Gene namesi
Name:Prox1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97772 Prox1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002088811 – 737Prospero homeobox protein 1Add BLAST737

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177PhosphoserineBy similarity1
Modified residuei179PhosphoserineCombined sources1
Modified residuei199PhosphoserineBy similarity1
Modified residuei291PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Cross-linki324Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei511PhosphoserineCombined sources1
Modified residuei514PhosphoserineCombined sources1
Modified residuei557PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP48437
PaxDbiP48437
PeptideAtlasiP48437
PRIDEiP48437

PTM databases

iPTMnetiP48437
PhosphoSitePlusiP48437

Expressioni

Tissue specificityi

Expressed in the young neurons of the subventricular region of the CNS, developing eye lens and pancreas. It is also found in the developing liver, heart and skeletal muscle. In the eye, expressed in the lens and retina at postnatal day 10. In the retina, localized to the inner nuclear layer. In the lens, localized to epithelial and fiber cells.2 Publications

Inductioni

Expressed in a circadian manner in the liver with a peak at ZT4.1 Publication

Gene expression databases

BgeeiENSMUSG00000010175
CleanExiMM_PROX1
GenevisibleiP48437 MM

Interactioni

Subunit structurei

Interacts with RORA and RORG (via AF-2 motif).1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202396, 3 interactors
DIPiDIP-58960N
IntActiP48437, 3 interactors
MINTiP48437
STRINGi10090.ENSMUSP00000010319

Structurei

3D structure databases

ProteinModelPortaliP48437
SMRiP48437
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini577 – 635Prospero-type homeoPROSITE-ProRule annotationAdd BLAST59
Domaini636 – 735ProsperoPROSITE-ProRule annotationAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 28Interaction with RORG1 PublicationAdd BLAST28
Regioni577 – 735Homeo-ProsperoPROSITE-ProRule annotationAdd BLAST159
Regioni723 – 729Essential for nuclear localization, interaction with RORG, repression of RORG transcriptional activator activity1 Publication7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi215 – 219Poly-Gln5

Domaini

The Prospero-type homeodomain and the adjacent Prospero domain act as a single structural unit, the Homeo-Prospero domain (Potential). The Prospero-type homeodomain is essential for repression of RORG transcriptional activator activity (PubMed:23723244).PROSITE-ProRule annotation1 Publication

Sequence similaritiesi

Belongs to the Prospero homeodomain family.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3779 Eukaryota
ENOG410ZE21 LUCA
GeneTreeiENSGT00530000063507
HOGENOMiHOG000115708
HOVERGENiHBG053693
InParanoidiP48437
KOiK20211
OMAiSCFMSRN
OrthoDBiEOG091G02KS
PhylomeDBiP48437
TreeFamiTF316638

Family and domain databases

Gene3Di1.10.10.500, 1 hit
InterProiView protein in InterPro
IPR023082 Homeo_prospero_dom
IPR037131 Homeo_prospero_dom_sf
IPR009057 Homeobox-like_sf
IPR007738 Prox1
PANTHERiPTHR12198:SF6 PTHR12198:SF6, 1 hit
PfamiView protein in Pfam
PF05044 HPD, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS51818 HOMEO_PROSPERO, 1 hit

Sequencei

Sequence statusi: Complete.

P48437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDHDSTALL SRQTKRRRVD IGVKRTVGTA SAFFAKARAT FFSAMNPQGS
60 70 80 90 100
EQDVEYSVVQ HADGEKSNVL RKLLKRANSY EDAMMPFPGA TIISQLLKNN
110 120 130 140 150
MNKNGGTEPS FQASGLSSTG SEVHQEDICS NSSRDSPPEC LSPFGRPTMS
160 170 180 190 200
QFDVDRLCDE HLRAKRARVE NIIRGMSHSP SVALRGNENE REMAPQSVSP
210 220 230 240 250
RESYRENKRK QKLPQQQQQS FQQLVSARKE QKREERRQLK QQLEDMQKQL
260 270 280 290 300
RQLQEKFYQV YDSTDSENDE DGDLSEDSMR SEILDARAQD SVGRSDNEMC
310 320 330 340 350
ELDPGQFIDR ARALIREQEM AENKPKREGS NKERDHGPNS LQPEGKHLAE
360 370 380 390 400
TLKQELNTAM SQVVDTVVKV FSAKPSRQVP QVFPPLQIPQ ARFAVNGENH
410 420 430 440 450
NFHTANQRLQ CFGDVIIPNP LDTFGSVQMP SSTDQTEALP LVVRKNSSEQ
460 470 480 490 500
SASGPATGGH HQPLHQSPLS ATAGFTTPSF RHPFPLPLMA YPFQSPLGAP
510 520 530 540 550
SGSFSGKDRA SPESLDLTRD TTSLRTKMSS HHLSHHPCSP AHPPSTAEGL
560 570 580 590 600
SLSLIKSECG DLQDMSDISP YSGSAMQEGL SPNHLKKAKL MFFYTRYPSS
610 620 630 640 650
NMLKTYFSDV KFNRCITSQL IKWFSNFREF YYIQMEKYAR QAINDGVTST
660 670 680 690 700
EELSITRDCE LYRALNMHYN KANDFEVPER FLEVAQITLR EFFNAIIAGK
710 720 730
DVDPSWKKAI YKVICKLDSE VPEIFKSPNC LQELLHE
Length:737
Mass (Da):83,126
Last modified:April 27, 2001 - v2
Checksum:i834DF6963FF5C9B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti685A → V in BAC35190 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061576 mRNA Translation: AAC32824.1
AK052889 mRNA Translation: BAC35190.1
AK143179 mRNA Translation: BAE25293.1
BC051411 mRNA Translation: AAH51411.1
CCDSiCCDS35822.1
PIRiJE0269
RefSeqiNP_032963.1, NM_008937.2
XP_006497191.1, XM_006497128.3
UniGeneiMm.132579
Mm.392678

Genome annotation databases

EnsembliENSMUST00000010319; ENSMUSP00000010319; ENSMUSG00000010175
ENSMUST00000175916; ENSMUSP00000135703; ENSMUSG00000010175
ENSMUST00000177288; ENSMUSP00000135066; ENSMUSG00000010175
GeneIDi19130
KEGGimmu:19130
UCSCiuc007ebc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPROX1_MOUSE
AccessioniPrimary (citable) accession number: P48437
Secondary accession number(s): O88478, Q3UPV1, Q543D8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health