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P48432 (SOX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor SOX-2
Gene names
Name:Sox2
Synonyms:Sox-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation By similarity. Ref.4 Ref.8

Subunit structure

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1. Ref.6 Ref.7

Subcellular location

Nucleus Ref.4.

Tissue specificity

Expressed in the brain and retina. A very low level expression is seen in the stomach and lung. Expressed in developing urogenital ridge.

Induction

By SRRT. Ref.8

Post-translational modification

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. Ref.4

Biotechnological use

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.5

Sequence similarities

Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenohypophysis development

Inferred from mutant phenotype PubMed 16932809. Source: MGI

anatomical structure formation involved in morphogenesis

Inferred from mutant phenotype PubMed 17522155PubMed 9851841. Source: MGI

cell fate commitment

Inferred from direct assay PubMed 18374910. Source: MGI

cell fate specification

Inferred from mutant phenotype PubMed 12514105. Source: MGI

cerebral cortex development

Inferred from mutant phenotype PubMed 15240551. Source: MGI

detection of mechanical stimulus involved in equilibrioception

Inferred from mutant phenotype PubMed 15846349. Source: MGI

detection of mechanical stimulus involved in sensory perception of sound

Inferred from mutant phenotype PubMed 15846349. Source: MGI

diencephalon morphogenesis

Inferred from mutant phenotype PubMed 15240551. Source: MGI

embryonic organ development

Inferred from mutant phenotype PubMed 17522155. Source: MGI

endodermal cell fate specification

Inferred from sequence orthology PubMed 21245162. Source: MGI

epithelial tube branching involved in lung morphogenesis

Inferred from direct assay PubMed 18374910. Source: MGI

forebrain neuron differentiation

Inferred from mutant phenotype PubMed 15240551. Source: MGI

inner ear morphogenesis

Inferred from mutant phenotype PubMed 15846349. Source: MGI

lens induction in camera-type eye

Inferred from genetic interaction PubMed 17140559. Source: MGI

lung alveolus development

Inferred from direct assay PubMed 18374910. Source: MGI

male genitalia development

Inferred from mutant phenotype PubMed 16932809. Source: MGI

negative regulation of Wnt signaling pathway

Inferred from genetic interaction PubMed 15781477. Source: MGI

negative regulation of canonical Wnt signaling pathway

Inferred from direct assay PubMed 17875931. Source: UniProtKB

negative regulation of cell differentiation

Inferred from mutant phenotype PubMed 17507372. Source: MGI

negative regulation of neuron differentiation

Inferred from direct assay PubMed 16631155. Source: MGI

negative regulation of osteoblast differentiation

Inferred from direct assay PubMed 15781477. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 9649510. Source: MGI

neuron fate commitment

Inferred from mutant phenotype PubMed 17015430. Source: MGI

neuronal stem cell maintenance

Inferred from genetic interaction Ref.8. Source: UniProtKB

olfactory placode formation

Inferred from genetic interaction PubMed 17140559. Source: MGI

pigment biosynthetic process

Inferred from mutant phenotype PubMed 12036291. Source: MGI

positive regulation of Notch signaling pathway

Inferred from direct assay PubMed 16631155. Source: MGI

positive regulation of epithelial cell differentiation

Inferred from direct assay PubMed 18374910. Source: MGI

positive regulation of neuroblast proliferation

Inferred from mutant phenotype PubMed 15240551PubMed 16651659. Source: MGI

positive regulation of neuron differentiation

Inferred from mutant phenotype PubMed 16651659. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16932809Ref.4PubMed 17507372PubMed 7628452. Source: MGI

regulation of neurogenesis

Inferred from genetic interaction PubMed 22513373. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19796622. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 12665572. Source: MGI

response to organic substance

Inferred from direct assay PubMed 18400104. Source: MGI

response to retinoic acid

Inferred from direct assay PubMed 18400104. Source: MGI

retina morphogenesis in camera-type eye

Inferred from mutant phenotype PubMed 16651659. Source: MGI

sensory perception of sound

Inferred from mutant phenotype PubMed 12036291. Source: MGI

stem cell differentiation

Inferred from direct assay PubMed 20123909. Source: MGI

stem cell maintenance

Inferred from mutant phenotype PubMed 16767105PubMed 20720539. Source: MGI

tongue development

Inferred from mutant phenotype PubMed 17015430. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 22344693. Source: GOC

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 12514105. Source: MGI

nuclear transcription factor complex

Inferred from direct assay PubMed 22344693. Source: MGI

nucleus

Inferred from direct assay PubMed 19490090. Source: UniProtKB

transcription factor complex

Inferred from direct assay PubMed 17507372. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12665572PubMed 15082719Ref.3. Source: MGI

chromatin binding

Inferred from direct assay PubMed 15988017. Source: MGI

protein binding

Inferred from physical interaction Ref.7. Source: UniProtKB

protein heterodimerization activity

Inferred from direct assay PubMed 22344693. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 18400104PubMed 7628452PubMed 9649510PubMed 9669521. Source: MGI

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from direct assay PubMed 22344693. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 12665572PubMed 16932809Ref.4PubMed 7628452PubMed 9669521. Source: MGI

transcription factor binding

Inferred from physical interaction PubMed 19816951. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from direct assay PubMed 19328208. Source: UniProtKB

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Transcription factor SOX-2
PRO_0000048716

Regions

DNA binding43 – 11169HMG box
Compositional bias19 – 257Poly-Gly

Amino acid modifications

Modified residue2531Phosphoserine By similarity
Cross-link247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.4

Experimental info

Mutagenesis2471K → R: Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization. Ref.4
Sequence conflict153 – 1553GGL → AGV in CAA63847. Ref.3
Sequence conflict2031V → D in CAA63847. Ref.3
Sequence conflict310 – 3112KY → IN in CAA63847. Ref.3

Secondary structure

....... 319
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P48432 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: C40DE49E524C49F1

FASTA31934,454
        10         20         30         40         50         60 
MYNMMETELK PPGPQQASGG GGGGGNATAA ATGGNQKNSP DRVKRPMNAF MVWSRGQRRK 

        70         80         90        100        110        120 
MAQENPKMHN SEISKRLGAE WKLLSETEKR PFIDEAKRLR ALHMKEHPDY KYRPRRKTKT 

       130        140        150        160        170        180 
LMKKDKYTLP GGLLAPGGNS MASGVGVGAG LGGGLNQRMD SYAHMNGWSN GSYSMMQEQL 

       190        200        210        220        230        240 
GYPQHPGLNA HGAAQMQPMH RYVVSALQYN SMTSSQTYMN GSPTYSMSYS QQGTPGMALG 

       250        260        270        280        290        300 
SMGSVVKSEA SSSPPVVTSS SHSRAPCQAG DLRDMISMYL PGAEVPEPAA PSRLHMAQHY 

       310 
QSGPVPGTAK YGTLPLSHM 

« Hide

References

[1]"Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3."
Yuan H., Corbi N., Basilico C., Dailey L.
Genes Dev. 9:2635-2645(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Yuan H., Corbi N., Basilico C., Dailey L.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2."
Collignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Rastan S., Stevanovic M., Goodfellow P.N., Lovell-Badge R.
Development 122:509-520(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[4]"Inhibition of DNA binding of Sox2 by the SUMO conjugation."
Tsuruzoe S., Ishihara K., Uchimura Y., Watanabe S., Sekita Y., Aoto T., Saitoh H., Yuasa Y., Niwa H., Kawasuji M., Baba H., Nakao M.
Biochem. Biophys. Res. Commun. 351:920-926(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION AT LYS-247, MUTAGENESIS OF LYS-247, SUBCELLULAR LOCATION, FUNCTION.
[5]"Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors."
Takahashi K., Yamanaka S.
Cell 126:663-676(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
[6]"SOX3 activity during pharyngeal segmentation is required for craniofacial morphogenesis."
Rizzoti K., Lovell-Badge R.
Development 134:3437-3448(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SOX3 AND FGFR1.
[7]"Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells."
Yu H.B., Kunarso G., Hong F.H., Stanton L.W.
J. Biol. Chem. 284:31327-31335(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZSCAN10.
[8]"Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2."
Andreu-Agullo C., Maurin T., Thompson C.B., Lai E.C.
Nature 481:195-198(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U31967 mRNA. Translation: AAC31791.1.
X94127 Genomic DNA. Translation: CAA63847.1.
CCDSCCDS38413.1.
PIRS10949.
RefSeqNP_035573.3. NM_011443.3.
UniGeneMm.65396.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GT0X-ray2.60D41-120[»]
ProteinModelPortalP48432.
SMRP48432. Positions 41-119.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203406. 21 interactions.
DIPDIP-54522N.
IntActP48432. 6 interactions.

PTM databases

PhosphoSiteP48432.

Proteomic databases

PRIDEP48432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID20674.
KEGGmmu:20674.

Organism-specific databases

CTD6657.
MGIMGI:98364. Sox2.

Phylogenomic databases

eggNOGNOG321816.
HOGENOMHOG000231647.
HOVERGENHBG105663.
InParanoidP48432.
KOK16796.
PhylomeDBP48432.

Gene expression databases

CleanExMM_SOX2.
GenevestigatorP48432.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
InterProIPR009071. HMG_box_dom.
IPR022097. TF_SOX.
[Graphical view]
PfamPF00505. HMG_box. 1 hit.
PF12336. SOXp. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP48432.
NextBio299161.
PROP48432.
SOURCESearch...

Entry information

Entry nameSOX2_MOUSE
AccessionPrimary (citable) accession number: P48432
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot