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P48432

- SOX2_MOUSE

UniProt

P48432 - SOX2_MOUSE

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Protein

Transcription factor SOX-2

Gene

Sox2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi43 – 11169HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI
  3. protein heterodimerization activity Source: MGI
  4. sequence-specific DNA binding Source: MGI
  5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
  6. sequence-specific DNA binding transcription factor activity Source: MGI
  7. transcription factor binding Source: UniProtKB
  8. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. adenohypophysis development Source: MGI
  2. anatomical structure formation involved in morphogenesis Source: MGI
  3. cell fate commitment Source: MGI
  4. cell fate specification Source: MGI
  5. cerebral cortex development Source: MGI
  6. detection of mechanical stimulus involved in equilibrioception Source: MGI
  7. detection of mechanical stimulus involved in sensory perception of sound Source: MGI
  8. diencephalon morphogenesis Source: MGI
  9. embryonic organ development Source: MGI
  10. endodermal cell fate specification Source: MGI
  11. epithelial tube branching involved in lung morphogenesis Source: MGI
  12. forebrain neuron differentiation Source: MGI
  13. inner ear morphogenesis Source: MGI
  14. lens induction in camera-type eye Source: MGI
  15. lung alveolus development Source: MGI
  16. male genitalia development Source: MGI
  17. negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  18. negative regulation of cell differentiation Source: MGI
  19. negative regulation of neuron differentiation Source: MGI
  20. negative regulation of osteoblast differentiation Source: MGI
  21. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  22. negative regulation of Wnt signaling pathway Source: MGI
  23. neuronal stem cell maintenance Source: UniProtKB
  24. neuron fate commitment Source: MGI
  25. olfactory placode formation Source: MGI
  26. pigment biosynthetic process Source: MGI
  27. positive regulation of epithelial cell differentiation Source: MGI
  28. positive regulation of neuroblast proliferation Source: MGI
  29. positive regulation of neuron differentiation Source: MGI
  30. positive regulation of Notch signaling pathway Source: MGI
  31. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  32. regulation of neurogenesis Source: MGI
  33. regulation of transcription, DNA-templated Source: MGI
  34. regulation of transcription from RNA polymerase II promoter Source: MGI
  35. response to organic substance Source: MGI
  36. response to retinoic acid Source: MGI
  37. retina morphogenesis in camera-type eye Source: MGI
  38. sensory perception of sound Source: MGI
  39. stem cell differentiation Source: MGI
  40. stem cell maintenance Source: MGI
  41. tongue development Source: MGI
  42. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SOX-2
Gene namesi
Name:Sox2
Synonyms:Sox-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:98364. Sox2.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nuclear transcription factor complex Source: MGI
  3. nucleus Source: UniProtKB
  4. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi247 – 2471K → R: Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Transcription factor SOX-2PRO_0000048716Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki247 – 247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei253 – 2531PhosphoserineBy similarity

Post-translational modificationi

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP48432.
PRIDEiP48432.

PTM databases

PhosphoSiteiP48432.

Expressioni

Tissue specificityi

Expressed in the brain and retina. A very low level expression is seen in the stomach and lung. Expressed in developing urogenital ridge.

Inductioni

By SRRT.1 Publication

Gene expression databases

CleanExiMM_SOX2.
GenevestigatoriP48432.

Interactioni

Subunit structurei

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NanogQ80Z6410EBI-2313612,EBI-2312517
Pou5f1P202634EBI-2313612,EBI-1606219
Sin3aQ605203EBI-2313612,EBI-349034

Protein-protein interaction databases

BioGridi203406. 23 interactions.
DIPiDIP-54522N.
IntActiP48432. 6 interactions.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi49 – 6214Combined sources
Helixi70 – 8112Combined sources
Helixi86 – 10621Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GT0X-ray2.60D41-120[»]
ProteinModelPortaliP48432.
SMRiP48432. Positions 41-119.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48432.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi19 – 257Poly-Gly

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG321816.
HOGENOMiHOG000231647.
HOVERGENiHBG105663.
InParanoidiP48432.
KOiK16796.
PhylomeDBiP48432.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
IPR022097. TF_SOX.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF12336. SOXp. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48432-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNMMETELK PPGPQQASGG GGGGGNATAA ATGGNQKNSP DRVKRPMNAF
60 70 80 90 100
MVWSRGQRRK MAQENPKMHN SEISKRLGAE WKLLSETEKR PFIDEAKRLR
110 120 130 140 150
ALHMKEHPDY KYRPRRKTKT LMKKDKYTLP GGLLAPGGNS MASGVGVGAG
160 170 180 190 200
LGGGLNQRMD SYAHMNGWSN GSYSMMQEQL GYPQHPGLNA HGAAQMQPMH
210 220 230 240 250
RYVVSALQYN SMTSSQTYMN GSPTYSMSYS QQGTPGMALG SMGSVVKSEA
260 270 280 290 300
SSSPPVVTSS SHSRAPCQAG DLRDMISMYL PGAEVPEPAA PSRLHMAQHY
310
QSGPVPGTAK YGTLPLSHM
Length:319
Mass (Da):34,454
Last modified:July 15, 1999 - v2
Checksum:iC40DE49E524C49F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1553GGL → AGV in CAA63847. (PubMed:8625802)Curated
Sequence conflicti203 – 2031V → D in CAA63847. (PubMed:8625802)Curated
Sequence conflicti310 – 3112KY → IN in CAA63847. (PubMed:8625802)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31967 mRNA. Translation: AAC31791.1.
X94127 Genomic DNA. Translation: CAA63847.1.
CCDSiCCDS38413.1.
PIRiS10949.
RefSeqiNP_035573.3. NM_011443.3.
UniGeneiMm.65396.

Genome annotation databases

GeneIDi20674.
KEGGimmu:20674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31967 mRNA. Translation: AAC31791.1 .
X94127 Genomic DNA. Translation: CAA63847.1 .
CCDSi CCDS38413.1.
PIRi S10949.
RefSeqi NP_035573.3. NM_011443.3.
UniGenei Mm.65396.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GT0 X-ray 2.60 D 41-120 [» ]
ProteinModelPortali P48432.
SMRi P48432. Positions 41-119.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203406. 23 interactions.
DIPi DIP-54522N.
IntActi P48432. 6 interactions.

PTM databases

PhosphoSitei P48432.

Proteomic databases

MaxQBi P48432.
PRIDEi P48432.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 20674.
KEGGi mmu:20674.

Organism-specific databases

CTDi 6657.
MGIi MGI:98364. Sox2.

Phylogenomic databases

eggNOGi NOG321816.
HOGENOMi HOG000231647.
HOVERGENi HBG105663.
InParanoidi P48432.
KOi K16796.
PhylomeDBi P48432.

Miscellaneous databases

EvolutionaryTracei P48432.
NextBioi 299161.
PROi P48432.
SOURCEi Search...

Gene expression databases

CleanExi MM_SOX2.
Genevestigatori P48432.

Family and domain databases

Gene3Di 1.10.30.10. 1 hit.
InterProi IPR009071. HMG_box_dom.
IPR022097. TF_SOX.
[Graphical view ]
Pfami PF00505. HMG_box. 1 hit.
PF12336. SOXp. 1 hit.
[Graphical view ]
SMARTi SM00398. HMG. 1 hit.
[Graphical view ]
SUPFAMi SSF47095. SSF47095. 1 hit.
PROSITEi PS50118. HMG_BOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3."
    Yuan H., Corbi N., Basilico C., Dailey L.
    Genes Dev. 9:2635-2645(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Yuan H., Corbi N., Basilico C., Dailey L.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2."
    Collignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Rastan S., Stevanovic M., Goodfellow P.N., Lovell-Badge R.
    Development 122:509-520(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  4. Cited for: SUMOYLATION AT LYS-247, MUTAGENESIS OF LYS-247, SUBCELLULAR LOCATION, FUNCTION.
  5. "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors."
    Takahashi K., Yamanaka S.
    Cell 126:663-676(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY.
  6. "SOX3 activity during pharyngeal segmentation is required for craniofacial morphogenesis."
    Rizzoti K., Lovell-Badge R.
    Development 134:3437-3448(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SOX3 AND FGFR1.
  7. "Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells."
    Yu H.B., Kunarso G., Hong F.H., Stanton L.W.
    J. Biol. Chem. 284:31327-31335(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZSCAN10.
  8. "Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2."
    Andreu-Agullo C., Maurin T., Thompson C.B., Lai E.C.
    Nature 481:195-198(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiSOX2_MOUSE
AccessioniPrimary (citable) accession number: P48432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3