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P48432

- SOX2_MOUSE

UniProt

P48432 - SOX2_MOUSE

Protein

Transcription factor SOX-2

Gene

Sox2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi43 – 11169HMG boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: MGI
    5. sequence-specific DNA binding Source: MGI
    6. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
    7. sequence-specific DNA binding transcription factor activity Source: MGI
    8. transcription factor binding Source: UniProtKB
    9. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. adenohypophysis development Source: MGI
    2. anatomical structure formation involved in morphogenesis Source: MGI
    3. cell fate commitment Source: MGI
    4. cell fate specification Source: MGI
    5. cerebral cortex development Source: MGI
    6. detection of mechanical stimulus involved in equilibrioception Source: MGI
    7. detection of mechanical stimulus involved in sensory perception of sound Source: MGI
    8. diencephalon morphogenesis Source: MGI
    9. embryonic organ development Source: MGI
    10. endodermal cell fate specification Source: MGI
    11. epithelial tube branching involved in lung morphogenesis Source: MGI
    12. forebrain neuron differentiation Source: MGI
    13. inner ear morphogenesis Source: MGI
    14. lens induction in camera-type eye Source: MGI
    15. lung alveolus development Source: MGI
    16. male genitalia development Source: MGI
    17. negative regulation of canonical Wnt signaling pathway Source: UniProtKB
    18. negative regulation of cell differentiation Source: MGI
    19. negative regulation of neuron differentiation Source: MGI
    20. negative regulation of osteoblast differentiation Source: MGI
    21. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    22. negative regulation of Wnt signaling pathway Source: MGI
    23. neuronal stem cell maintenance Source: UniProtKB
    24. neuron fate commitment Source: MGI
    25. olfactory placode formation Source: MGI
    26. pigment biosynthetic process Source: MGI
    27. positive regulation of epithelial cell differentiation Source: MGI
    28. positive regulation of neuroblast proliferation Source: MGI
    29. positive regulation of neuron differentiation Source: MGI
    30. positive regulation of Notch signaling pathway Source: MGI
    31. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    32. regulation of neurogenesis Source: MGI
    33. regulation of transcription, DNA-templated Source: MGI
    34. regulation of transcription from RNA polymerase II promoter Source: MGI
    35. response to organic substance Source: MGI
    36. response to retinoic acid Source: MGI
    37. retina morphogenesis in camera-type eye Source: MGI
    38. sensory perception of sound Source: MGI
    39. stem cell differentiation Source: MGI
    40. stem cell maintenance Source: MGI
    41. tongue development Source: MGI
    42. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor SOX-2
    Gene namesi
    Name:Sox2
    Synonyms:Sox-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:98364. Sox2.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nuclear transcription factor complex Source: MGI
    3. nucleus Source: UniProtKB
    4. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Biotechnological usei

    POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi247 – 2471K → R: Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 319319Transcription factor SOX-2PRO_0000048716Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki247 – 247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
    Modified residuei253 – 2531PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP48432.

    PTM databases

    PhosphoSiteiP48432.

    Expressioni

    Tissue specificityi

    Expressed in the brain and retina. A very low level expression is seen in the stomach and lung. Expressed in developing urogenital ridge.

    Inductioni

    By SRRT.1 Publication

    Gene expression databases

    CleanExiMM_SOX2.
    GenevestigatoriP48432.

    Interactioni

    Subunit structurei

    Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NanogQ80Z6410EBI-2313612,EBI-2312517
    Pou5f1P202634EBI-2313612,EBI-1606219
    Sin3aQ605203EBI-2313612,EBI-349034

    Protein-protein interaction databases

    BioGridi203406. 21 interactions.
    DIPiDIP-54522N.
    IntActiP48432. 6 interactions.

    Structurei

    Secondary structure

    1
    319
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi49 – 6214
    Helixi70 – 8112
    Helixi86 – 10621

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GT0X-ray2.60D41-120[»]
    ProteinModelPortaliP48432.
    SMRiP48432. Positions 41-119.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP48432.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi19 – 257Poly-Gly

    Sequence similaritiesi

    Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG321816.
    HOGENOMiHOG000231647.
    HOVERGENiHBG105663.
    InParanoidiP48432.
    KOiK16796.
    PhylomeDBiP48432.

    Family and domain databases

    Gene3Di1.10.30.10. 1 hit.
    InterProiIPR009071. HMG_box_dom.
    IPR022097. TF_SOX.
    [Graphical view]
    PfamiPF00505. HMG_box. 1 hit.
    PF12336. SOXp. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 1 hit.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 1 hit.
    PROSITEiPS50118. HMG_BOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48432-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNMMETELK PPGPQQASGG GGGGGNATAA ATGGNQKNSP DRVKRPMNAF    50
    MVWSRGQRRK MAQENPKMHN SEISKRLGAE WKLLSETEKR PFIDEAKRLR 100
    ALHMKEHPDY KYRPRRKTKT LMKKDKYTLP GGLLAPGGNS MASGVGVGAG 150
    LGGGLNQRMD SYAHMNGWSN GSYSMMQEQL GYPQHPGLNA HGAAQMQPMH 200
    RYVVSALQYN SMTSSQTYMN GSPTYSMSYS QQGTPGMALG SMGSVVKSEA 250
    SSSPPVVTSS SHSRAPCQAG DLRDMISMYL PGAEVPEPAA PSRLHMAQHY 300
    QSGPVPGTAK YGTLPLSHM 319
    Length:319
    Mass (Da):34,454
    Last modified:July 15, 1999 - v2
    Checksum:iC40DE49E524C49F1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti153 – 1553GGL → AGV in CAA63847. (PubMed:8625802)Curated
    Sequence conflicti203 – 2031V → D in CAA63847. (PubMed:8625802)Curated
    Sequence conflicti310 – 3112KY → IN in CAA63847. (PubMed:8625802)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31967 mRNA. Translation: AAC31791.1.
    X94127 Genomic DNA. Translation: CAA63847.1.
    CCDSiCCDS38413.1.
    PIRiS10949.
    RefSeqiNP_035573.3. NM_011443.3.
    UniGeneiMm.65396.

    Genome annotation databases

    GeneIDi20674.
    KEGGimmu:20674.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31967 mRNA. Translation: AAC31791.1 .
    X94127 Genomic DNA. Translation: CAA63847.1 .
    CCDSi CCDS38413.1.
    PIRi S10949.
    RefSeqi NP_035573.3. NM_011443.3.
    UniGenei Mm.65396.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GT0 X-ray 2.60 D 41-120 [» ]
    ProteinModelPortali P48432.
    SMRi P48432. Positions 41-119.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203406. 21 interactions.
    DIPi DIP-54522N.
    IntActi P48432. 6 interactions.

    PTM databases

    PhosphoSitei P48432.

    Proteomic databases

    PRIDEi P48432.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 20674.
    KEGGi mmu:20674.

    Organism-specific databases

    CTDi 6657.
    MGIi MGI:98364. Sox2.

    Phylogenomic databases

    eggNOGi NOG321816.
    HOGENOMi HOG000231647.
    HOVERGENi HBG105663.
    InParanoidi P48432.
    KOi K16796.
    PhylomeDBi P48432.

    Miscellaneous databases

    EvolutionaryTracei P48432.
    NextBioi 299161.
    PROi P48432.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_SOX2.
    Genevestigatori P48432.

    Family and domain databases

    Gene3Di 1.10.30.10. 1 hit.
    InterProi IPR009071. HMG_box_dom.
    IPR022097. TF_SOX.
    [Graphical view ]
    Pfami PF00505. HMG_box. 1 hit.
    PF12336. SOXp. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 1 hit.
    PROSITEi PS50118. HMG_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3."
      Yuan H., Corbi N., Basilico C., Dailey L.
      Genes Dev. 9:2635-2645(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Yuan H., Corbi N., Basilico C., Dailey L.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2."
      Collignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Rastan S., Stevanovic M., Goodfellow P.N., Lovell-Badge R.
      Development 122:509-520(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129.
    4. Cited for: SUMOYLATION AT LYS-247, MUTAGENESIS OF LYS-247, SUBCELLULAR LOCATION, FUNCTION.
    5. "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors."
      Takahashi K., Yamanaka S.
      Cell 126:663-676(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY.
    6. "SOX3 activity during pharyngeal segmentation is required for craniofacial morphogenesis."
      Rizzoti K., Lovell-Badge R.
      Development 134:3437-3448(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SOX3 AND FGFR1.
    7. "Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells."
      Yu H.B., Kunarso G., Hong F.H., Stanton L.W.
      J. Biol. Chem. 284:31327-31335(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZSCAN10.
    8. "Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2."
      Andreu-Agullo C., Maurin T., Thompson C.B., Lai E.C.
      Nature 481:195-198(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiSOX2_MOUSE
    AccessioniPrimary (citable) accession number: P48432
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3