P48432 (SOX2_MOUSE)
Reviewed,
UniProtKB/Swiss-Prot
Last modified
August 10, 2010.
Version 83.
History...
Names and origin
| Protein names | Recommended name: Transcription factor SOX-2 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 319 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Ref.4 |
| Subunit structure | Interacts with ZSCAN10. Ref.5 |
| Subcellular location | |
| Tissue specificity | Expressed in the brain and retina. A very low level expression is seen in the stomach and lung. Expressed in developing urogenital ridge. |
| Post-translational modification | Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. |
| Sequence similarities | Contains 1 HMG box DNA-binding domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||
Molecule processing | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 319 | 319 | Transcription factor SOX-2 | PRO_0000048716 | |||||||||||
Regions | |||||||||||||||
| DNA binding | 43 – 111 | 69 | HMG box | ||||||||||||
| Compositional bias | 19 – 25 | 7 | Poly-Gly | ||||||||||||
Amino acid modifications | |||||||||||||||
| Cross-link | 247 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.4 | |||||||||||||
Experimental info | |||||||||||||||
| Mutagenesis | 247 | 1 | K → R: Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization. Ref.4 | ||||||||||||
| Sequence conflict | 153 – 155 | 3 | GGL → AGV in CAA63847. Ref.3 | ||||||||||||
| Sequence conflict | 203 | 1 | V → D in CAA63847. Ref.3 | ||||||||||||
| Sequence conflict | 310 – 311 | 2 | KY → IN in CAA63847. Ref.3 | ||||||||||||
Secondary structure | |||||||||||||||
Helix Strand Turn | |||||||||||||||
| Helix | 49 – 62 | 14 | |||||||||||||
| Helix | 70 – 81 | 12 | |||||||||||||
| Helix | 86 – 106 | 21 | |||||||||||||
Sequences
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References
| [1] | "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." Yuan H., Corbi N., Basilico C., Dailey L. Genes Dev. 9:2635-2645(1995) [PubMed: 7590241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | Yuan H., Corbi N., Basilico C., Dailey L. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. |
| [3] | "A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2." Collignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Rastan S., Stevanovic M., Goodfellow P.N., Lovell-Badge R. Development 122:509-520(1996) [PubMed: 8625802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129. |
| [4] | "Inhibition of DNA binding of Sox2 by the SUMO conjugation." Tsuruzoe S., Ishihara K., Uchimura Y., Watanabe S., Sekita Y., Aoto T., Saitoh H., Yuasa Y., Niwa H., Kawasuji M., Baba H., Nakao M. Biochem. Biophys. Res. Commun. 351:920-926(2006) [PubMed: 17097055] [Abstract] Cited for: SUMOYLATION AT LYS-247, MUTAGENESIS OF LYS-247, SUBCELLULAR LOCATION, FUNCTION. |
| [5] | "Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells." Yu H.B., Kunarso G., Hong F.H., Stanton L.W. J. Biol. Chem. 284:31327-31335(2009) [PubMed: 19740739] [Abstract] Cited for: INTERACTION WITH ZSCAN10. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | U31967 mRNA. Translation: AAC31791.1. X94127 Genomic DNA. Translation: CAA63847.1. | ||||||||||||
| IPI | IPI00319017. | ||||||||||||
| PIR | S10949. | ||||||||||||
| RefSeq | NP_035573.3. | ||||||||||||
| UniGene | Mm.65396. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||
| ProteinModelPortal | P48432. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P48432. 7 interactions. | ||||||||||||
| STRING | P48432. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | P48432. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | P48432. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSMUST00000099151; ENSMUSP00000096755; ENSMUSG00000074637; Mus musculus. [Genome view] | ||||||||||||
| GeneID | 20674. | ||||||||||||
| KEGG | mmu:20674. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 20674. | ||||||||||||
| MGI | MGI:98364. Sox2. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | roNOG07798. | ||||||||||||
| HOGENOM | HBG446398. | ||||||||||||
| HOVERGEN | HBG105663. | ||||||||||||
| InParanoid | P48432. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P48432. | ||||||||||||
| Bgee | P48432. | ||||||||||||
| CleanEx | MM_SOX2. | ||||||||||||
| Genevestigator | P48432. | ||||||||||||
| GermOnline | ENSMUSG00000074637. Mus musculus. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR000910. HMG_HMG1/HMG2. IPR009071. HMG_superfamily. IPR022097. TF_SOX. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:1.10.30.10. HMG-box. 1 hit. | ||||||||||||
| Pfam | PF00505. HMG_box. 1 hit. PF12336. SOXp. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00398. HMG. 1 hit. [Graphical view] | ||||||||||||
| SUPFAM | SSF47095. HMG-box. 1 hit. | ||||||||||||
| PROSITE | PS50118. HMG_BOX_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | SOX2_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P48432 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


