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Protein

Transcription factor SOX-2

Gene

Sox2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi43 – 111HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • chromatin binding Source: MGI
  • chromatin DNA binding Source: MGI
  • DNA binding Source: MGI
  • miRNA binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • adenohypophysis development Source: MGI
  • anatomical structure formation involved in morphogenesis Source: MGI
  • cell cycle arrest Source: MGI
  • cell fate commitment Source: MGI
  • cell fate specification Source: MGI
  • cerebral cortex development Source: MGI
  • detection of mechanical stimulus involved in equilibrioception Source: MGI
  • detection of mechanical stimulus involved in sensory perception of sound Source: MGI
  • diencephalon morphogenesis Source: MGI
  • embryonic organ development Source: MGI
  • endodermal cell fate specification Source: MGI
  • epithelial tube branching involved in lung morphogenesis Source: MGI
  • forebrain neuron differentiation Source: MGI
  • inner ear morphogenesis Source: MGI
  • lens induction in camera-type eye Source: MGI
  • lung alveolus development Source: MGI
  • male genitalia development Source: MGI
  • negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  • negative regulation of cell differentiation Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of osteoblast differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of Wnt signaling pathway Source: MGI
  • neuronal stem cell population maintenance Source: UniProtKB
  • neuron fate commitment Source: MGI
  • olfactory placode formation Source: MGI
  • osteoblast differentiation Source: MGI
  • pigment biosynthetic process Source: MGI
  • positive regulation of epithelial cell differentiation Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of neuroblast proliferation Source: MGI
  • positive regulation of neuron differentiation Source: MGI
  • positive regulation of Notch signaling pathway Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of neurogenesis Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to growth factor Source: MGI
  • response to organic substance Source: MGI
  • response to retinoic acid Source: MGI
  • retina morphogenesis in camera-type eye Source: MGI
  • sensory perception of sound Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • stem cell differentiation Source: MGI
  • stem cell population maintenance Source: MGI
  • tongue development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SOX-2
Gene namesi
Name:Sox2
Synonyms:Sox-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98364. Sox2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • nuclear transcription factor complex Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi247K → R: Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000487161 – 319Transcription factor SOX-2Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei253PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP48432.
PaxDbiP48432.
PeptideAtlasiP48432.
PRIDEiP48432.

PTM databases

iPTMnetiP48432.
PhosphoSitePlusiP48432.

Expressioni

Tissue specificityi

Expressed in the brain and retina. A very low level expression is seen in the stomach and lung. Expressed in developing urogenital ridge.

Inductioni

By SRRT.1 Publication

Gene expression databases

CleanExiMM_SOX2.

Interactioni

Subunit structurei

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NanogQ80Z6410EBI-2313612,EBI-2312517
Pou5f1P202634EBI-2313612,EBI-1606219
Sin3aQ605203EBI-2313612,EBI-349034

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi203406. 24 interactors.
DIPiDIP-54522N.
IntActiP48432. 6 interactors.
STRINGi10090.ENSMUSP00000096755.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi49 – 62Combined sources14
Helixi70 – 81Combined sources12
Helixi86 – 106Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GT0X-ray2.60D41-120[»]
ProteinModelPortaliP48432.
SMRiP48432.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48432.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi19 – 25Poly-Gly7

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IPZI. Eukaryota.
ENOG411009V. LUCA.
HOGENOMiHOG000231647.
HOVERGENiHBG105663.
InParanoidiP48432.
KOiK16796.
PhylomeDBiP48432.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
IPR032643. SOX-2.
IPR022097. SOX_fam.
[Graphical view]
PANTHERiPTHR10270:SF231. PTHR10270:SF231. 1 hit.
PfamiPF00505. HMG_box. 1 hit.
PF12336. SOXp. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNMMETELK PPGPQQASGG GGGGGNATAA ATGGNQKNSP DRVKRPMNAF
60 70 80 90 100
MVWSRGQRRK MAQENPKMHN SEISKRLGAE WKLLSETEKR PFIDEAKRLR
110 120 130 140 150
ALHMKEHPDY KYRPRRKTKT LMKKDKYTLP GGLLAPGGNS MASGVGVGAG
160 170 180 190 200
LGGGLNQRMD SYAHMNGWSN GSYSMMQEQL GYPQHPGLNA HGAAQMQPMH
210 220 230 240 250
RYVVSALQYN SMTSSQTYMN GSPTYSMSYS QQGTPGMALG SMGSVVKSEA
260 270 280 290 300
SSSPPVVTSS SHSRAPCQAG DLRDMISMYL PGAEVPEPAA PSRLHMAQHY
310
QSGPVPGTAK YGTLPLSHM
Length:319
Mass (Da):34,454
Last modified:July 15, 1999 - v2
Checksum:iC40DE49E524C49F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153 – 155GGL → AGV in CAA63847 (PubMed:8625802).Curated3
Sequence conflicti203V → D in CAA63847 (PubMed:8625802).Curated1
Sequence conflicti310 – 311KY → IN in CAA63847 (PubMed:8625802).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31967 mRNA. Translation: AAC31791.1.
X94127 Genomic DNA. Translation: CAA63847.1.
CCDSiCCDS38413.1.
PIRiS10949.
RefSeqiNP_035573.3. NM_011443.4.
UniGeneiMm.65396.

Genome annotation databases

GeneIDi20674.
KEGGimmu:20674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31967 mRNA. Translation: AAC31791.1.
X94127 Genomic DNA. Translation: CAA63847.1.
CCDSiCCDS38413.1.
PIRiS10949.
RefSeqiNP_035573.3. NM_011443.4.
UniGeneiMm.65396.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GT0X-ray2.60D41-120[»]
ProteinModelPortaliP48432.
SMRiP48432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203406. 24 interactors.
DIPiDIP-54522N.
IntActiP48432. 6 interactors.
STRINGi10090.ENSMUSP00000096755.

PTM databases

iPTMnetiP48432.
PhosphoSitePlusiP48432.

Proteomic databases

MaxQBiP48432.
PaxDbiP48432.
PeptideAtlasiP48432.
PRIDEiP48432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20674.
KEGGimmu:20674.

Organism-specific databases

CTDi6657.
MGIiMGI:98364. Sox2.

Phylogenomic databases

eggNOGiENOG410IPZI. Eukaryota.
ENOG411009V. LUCA.
HOGENOMiHOG000231647.
HOVERGENiHBG105663.
InParanoidiP48432.
KOiK16796.
PhylomeDBiP48432.

Miscellaneous databases

EvolutionaryTraceiP48432.
PROiP48432.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SOX2.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
IPR032643. SOX-2.
IPR022097. SOX_fam.
[Graphical view]
PANTHERiPTHR10270:SF231. PTHR10270:SF231. 1 hit.
PfamiPF00505. HMG_box. 1 hit.
PF12336. SOXp. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSOX2_MOUSE
AccessioniPrimary (citable) accession number: P48432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.