P48432 (SOX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor SOX-2

Gene names

Name:	Sox2
Synonyms:	Sox-2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Ref.4
Subunit structure	Interacts with ZSCAN10. Ref.5
Subcellular location	Nucleus Ref.4.
Tissue specificity	Expressed in the brain and retina. A very low level expression is seen in the stomach and lung. Expressed in developing urogenital ridge.
Post-translational modification	Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Sequence similarities	Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Isopeptide bond Ubl conjugation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	adenohypophysis development Inferred from mutant phenotype. Source: MGI cell fate specification Inferred from mutant phenotype. Source: MGI cerebral cortex development Inferred from mutant phenotype. Source: MGI detection of mechanical stimulus involved in equilibrioception Inferred from mutant phenotype. Source: MGI detection of mechanical stimulus involved in sensory perception of sound Inferred from mutant phenotype. Source: MGI diencephalon morphogenesis Inferred from mutant phenotype. Source: MGI epithelial tube branching involved in lung morphogenesis Inferred from direct assay. Source: MGI forebrain neuron differentiation Inferred from mutant phenotype. Source: MGI inner ear morphogenesis Inferred from mutant phenotype. Source: MGI lens induction in camera-type eye Inferred from genetic interaction. Source: MGI lung alveolus development Inferred from direct assay. Source: MGI male genitalia development Inferred from mutant phenotype. Source: MGI negative regulation of neuron differentiation Inferred from direct assay. Source: MGI negative regulation of osteoblast differentiation Inferred from direct assay. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction. Source: MGI neuron fate commitment Inferred from mutant phenotype. Source: MGI olfactory placode formation Inferred from genetic interaction. Source: MGI pigment biosynthetic process Inferred from mutant phenotype. Source: MGI positive regulation of Notch signaling pathway Inferred from direct assay. Source: MGI positive regulation of epithelial cell differentiation Inferred from direct assay. Source: MGI positive regulation of neuroblast proliferation Inferred from mutant phenotype. Source: MGI positive regulation of neuron differentiation Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Ref.4 Inferred from direct assay. Source: MGI response to retinoic acid Inferred from direct assay. Source: MGI retina morphogenesis in camera-type eye Inferred from mutant phenotype. Source: MGI tongue development Inferred from mutant phenotype. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	chromatin binding Inferred from direct assay. Source: MGI protein binding Ref.5 Inferred from physical interaction. Source: UniProtKB transcription factor activity Ref.4 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 319

319

Transcription factor SOX-2

PRO_0000048716

Regions

DNA binding

43 – 111

69

HMG box

Compositional bias

19 – 25

7

Poly-Gly

Amino acid modifications

Cross-link

247

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.4

Experimental info

Mutagenesis

247

1

K → R: Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization. Ref.4

Sequence conflict

153 – 155

3

GGL → AGV in CAA63847. Ref.3

Sequence conflict

203

1

V → D in CAA63847. Ref.3

Sequence conflict

310 – 311

2

KY → IN in CAA63847. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

319

Helix Strand Turn

Details...

Helix

49 – 62

14

Helix

70 – 81

12

Helix

86 – 106

21

Sequences

	Sequence		Length	Mass (Da)
	P48432-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: C40DE49E524C49F1	FASTA	319	34,454
	10 20 30 40 50 60 MYNMMETELK PPGPQQASGG GGGGGNATAA ATGGNQKNSP DRVKRPMNAF MVWSRGQRRK 70 80 90 100 110 120 MAQENPKMHN SEISKRLGAE WKLLSETEKR PFIDEAKRLR ALHMKEHPDY KYRPRRKTKT 130 140 150 160 170 180 LMKKDKYTLP GGLLAPGGNS MASGVGVGAG LGGGLNQRMD SYAHMNGWSN GSYSMMQEQL 190 200 210 220 230 240 GYPQHPGLNA HGAAQMQPMH RYVVSALQYN SMTSSQTYMN GSPTYSMSYS QQGTPGMALG 250 260 270 280 290 300 SMGSVVKSEA SSSPPVVTSS SHSRAPCQAG DLRDMISMYL PGAEVPEPAA PSRLHMAQHY 310 QSGPVPGTAK YGTLPLSHM

References

[1]	"Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." Yuan H., Corbi N., Basilico C., Dailey L. Genes Dev. 9:2635-2645(1995) [PubMed: 7590241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Yuan H., Corbi N., Basilico C., Dailey L. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2." Collignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Rastan S., Stevanovic M., Goodfellow P.N., Lovell-Badge R. Development 122:509-520(1996) [PubMed: 8625802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[4]	"Inhibition of DNA binding of Sox2 by the SUMO conjugation." Tsuruzoe S., Ishihara K., Uchimura Y., Watanabe S., Sekita Y., Aoto T., Saitoh H., Yuasa Y., Niwa H., Kawasuji M., Baba H., Nakao M. Biochem. Biophys. Res. Commun. 351:920-926(2006) [PubMed: 17097055] [Abstract] Cited for: SUMOYLATION AT LYS-247, MUTAGENESIS OF LYS-247, SUBCELLULAR LOCATION, FUNCTION.
[5]	"Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells." Yu H.B., Kunarso G., Hong F.H., Stanton L.W. J. Biol. Chem. 284:31327-31335(2009) [PubMed: 19740739] [Abstract] Cited for: INTERACTION WITH ZSCAN10.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U31967 mRNA. Translation: AAC31791.1.
X94127 Genomic DNA. Translation: CAA63847.1.

IPI

IPI00319017.

PIR

S10949.

RefSeq

NP_035573.3.

UniGene

Mm.65396.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GT0	X-ray	2.60	D	41-120	[»]

ProteinModelPortal

P48432.

ModBase

Search...

Protein-protein interaction databases

IntAct

P48432. 7 interactions.

STRING

P48432.

PTM databases

PhosphoSite

P48432.

Proteomic databases

PRIDE

P48432.

Genome annotation databases

Ensembl

ENSMUST00000099151; ENSMUSP00000096755; ENSMUSG00000074637; Mus musculus. [Genome view]

GeneID

20674.

KEGG

mmu:20674.

Organism-specific databases

CTD

20674.

MGI

MGI:98364. Sox2.

Phylogenomic databases

eggNOG

roNOG07798.

HOGENOM

HBG446398.

HOVERGEN

HBG105663.

InParanoid

P48432.

Gene expression databases

ArrayExpress

P48432.

Bgee

P48432.

CleanEx

MM_SOX2.

Genevestigator

P48432.

GermOnline

ENSMUSG00000074637. Mus musculus.

Family and domain databases

InterPro

IPR000910. HMG_HMG1/HMG2.
IPR009071. HMG_superfamily.
IPR022097. TF_SOX.
[Graphical view]

Gene3D

G3DSA:1.10.30.10. HMG-box. 1 hit.

Pfam

PF00505. HMG_box. 1 hit.
PF12336. SOXp. 1 hit.
[Graphical view]

SMART

SM00398. HMG. 1 hit.
[Graphical view]

SUPFAM

SSF47095. HMG-box. 1 hit.

PROSITE

PS50118. HMG_BOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

SOX2_MOUSE

Accession

Primary (citable) accession number: P48432

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	July 15, 1999
Last modified:	August 10, 2010
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)