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Protein

Flavonoid 3',5'-hydroxylase 1

Gene

CYP75A1

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin.

Catalytic activityi

A flavanone + 2 NADPH + 2 O2 = a 3',5'-dihydroxyflavanone + 2 NADP+ + 2 H2O.

Cofactori

hemeBy similarity

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi443Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12020.
BRENDAi1.14.13.88. 4700.
UniPathwayiUPA00009.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonoid 3',5'-hydroxylase 1 (EC:1.14.13.88)
Short name:
F3'5'H
Alternative name(s):
CYPLXXVA1
Cytochrome P450 75A1
Gene namesi
Name:CYP75A1
Synonyms:HF1
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521291 – 506Flavonoid 3',5'-hydroxylase 1Add BLAST506

Expressioni

Tissue specificityi

Flowers.

Structurei

3D structure databases

ProteinModelPortaliP48418.
SMRiP48418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

P48418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLLTELGAA TSIFLIAHII ISTLISKTTG RHLPPGPRGW PVIGALPLLG
60 70 80 90 100
AMPHVSLAKM AKKYGAIMYL KVGTCGMAVA STPDAAKAFL KTLDINFSNR
110 120 130 140 150
PPNAGATHLA YNAQDMVFAH YGPRWKLLRK LSNLHMLGGK ALENWANVRA
160 170 180 190 200
NELGHMLKSM SDMSREGQRV VVAEMLTFAM ANMIGQVMLS KRVFVDKGVE
210 220 230 240 250
VNEFKDMVVE LMTIAGYFNI GDFIPCLAWM DLQGIEKRMK RLHKKFDALL
260 270 280 290 300
TKMFDEHKAT TYERKGKPDF LDVVMENGDN SEGERLSTTN IKALLLNLFT
310 320 330 340 350
AGTDTSSSAI EWALAEMMKN PAILKKAQAE MDQVIGRNRR LLESDIPNLP
360 370 380 390 400
YLRAICKETF RKHPSTPLNL PRISNEPCIV DGYYIPKNTR LSVNIWAIGR
410 420 430 440 450
DPQVWENPLE FNPERFLSGR NSKIDPRGND FELIPFGAGR RICAGTRMGI
460 470 480 490 500
VMVEYILGTL VHSFDWKLPS EVIELNMEEA FGLALQKAVP LEAMVTPRLQ

LDVYVP
Length:506
Mass (Da):56,548
Last modified:February 1, 1996 - v1
Checksum:iFCF3F9623BD82B3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22545 mRNA. Translation: CAA80266.1.
D14588 mRNA. Translation: BAA03438.1.
AF081575 Genomic DNA. Translation: AAC32274.1.
AY245545 mRNA. Translation: AAO91941.1.
X71130 mRNA. Translation: CAA50442.1.
PIRiS32110.
S38985.

Similar proteinsi

Entry informationi

Entry nameiC75A1_PETHY
AccessioniPrimary (citable) accession number: P48418
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families