Reviewed, UniProtKB/Swiss-Prot P48418 (C75A1_PETHY)

Names and origin

Protein names

Recommended name:
    Flavonoid 3',5'-hydroxylase 1
      Short name=F3'5'H
    EC=1.14.13.88
Alternative name(s):
    Cytochrome P450 75A1
    CYPLXXVA1

Gene names

Name:	CYP75A1
Synonyms:	HF1

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin.
Catalytic activity	A flavanone + NADPH + O2 = a 3'-hydroxyflavanone + NADP+ + H2O. A 3'-hydroxyflavanone + NADPH + O2 = a 3',5'-dihydroxyflavanone + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Subcellular location	Microsome Potential. Endoplasmic reticulum By similarity.
Tissue specificity	Flowers.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavonoid 3',5'-hydroxylase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Flavonoid 3',5'-hydroxylase 1		PRO_0000052129
Sites
	Metal binding	443	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48418-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: FCF3F9623BD82B3E	FASTA	506	56,548
	10 20 30 40 50 60 MMLLTELGAA TSIFLIAHII ISTLISKTTG RHLPPGPRGW PVIGALPLLG AMPHVSLAKM 70 80 90 100 110 120 AKKYGAIMYL KVGTCGMAVA STPDAAKAFL KTLDINFSNR PPNAGATHLA YNAQDMVFAH 130 140 150 160 170 180 YGPRWKLLRK LSNLHMLGGK ALENWANVRA NELGHMLKSM SDMSREGQRV VVAEMLTFAM 190 200 210 220 230 240 ANMIGQVMLS KRVFVDKGVE VNEFKDMVVE LMTIAGYFNI GDFIPCLAWM DLQGIEKRMK 250 260 270 280 290 300 RLHKKFDALL TKMFDEHKAT TYERKGKPDF LDVVMENGDN SEGERLSTTN IKALLLNLFT 310 320 330 340 350 360 AGTDTSSSAI EWALAEMMKN PAILKKAQAE MDQVIGRNRR LLESDIPNLP YLRAICKETF 370 380 390 400 410 420 RKHPSTPLNL PRISNEPCIV DGYYIPKNTR LSVNIWAIGR DPQVWENPLE FNPERFLSGR 430 440 450 460 470 480 NSKIDPRGND FELIPFGAGR RICAGTRMGI VMVEYILGTL VHSFDWKLPS EVIELNMEEA 490 500 FGLALQKAVP LEAMVTPRLQ LDVYVP

References

[1]	"Cloning and expression of cytochrome P450 genes controlling flower colour." Holton T.A., Brugliera F., Lester D.R., Tanaka Y., Hyland C.D., Menting J.G.T., Lu C.-Y., Farcy E., Stevenson T.W., Cornish E.C. Nature 366:276-279(1993) [PubMed: 8232589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Old Glory Blue. Tissue: Petal.
[2]	"Molecular cloning of cDNA encoding flavonoid-3',5'-hydroxylase from Petunia hybrida." Ohbayashi M., Shimada Y., Nakano R., Kiyokawa S., Kikuchi Y. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Falcon Blue. Tissue: Petal.
[3]	"Cloning and sequencing of the genomic flavonoid 3',5'-hydroxylase (F3',5'Hase) gene from Petunia hybrida." Hwang T.S., Chen Y.C., Wu R.Y. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Isolation and characterization of cytochrome P450 genes." Baek S.H. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Carnival violet. Tissue: Petal.
[5]	"The cloning and characterization of a cDNA encoding a cytochrome P450 from the flowers of Petunia hybrida." Toguri T., Azuma M., Ohtani T. Plant Sci. 94:119-126(1993) Cited for: NUCLEOTIDE SEQUENCE OF 1-425. Strain: cv. Blue-star. Tissue: Flower.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22545 mRNA. Translation: CAA80266.1. D14588 mRNA. Translation: BAA03438.1. AF081575 Genomic DNA. Translation: AAC32274.1. AY245545 mRNA. Translation: AAO91941.1. X71130 mRNA. Translation: CAA50442.1.
PIR	S32110. S38985.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12020.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C75A1_PETHY

Accession

Primary (citable) accession number: P48418

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)