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Protein

Nonsense-mediated mRNA decay protein 3

Gene

UPF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nonsense-mediated decay of mRNAs containing premature stop codons.

GO - Molecular functioni

GO - Biological processi

  • DNA recombination Source: SGD
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay

Enzyme and pathway databases

BioCyciYEAST:G3O-30785-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nonsense-mediated mRNA decay protein 3
Alternative name(s):
Up-frameshift suppressor 3
Gene namesi
Name:UPF3
Synonyms:SUA6
Ordered Locus Names:YGR072W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR072W.
SGDiS000003304. UPF3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleolus Source: SGD
  • nucleus Source: SGD
  • polysome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Nonsense-mediated mRNA decay protein 3PRO_0000215298Add
BLAST

Proteomic databases

MaxQBiP48412.

Interactioni

Protein-protein interaction databases

BioGridi33315. 128 interactions.
DIPiDIP-2294N.
IntActiP48412. 2 interactions.
MINTiMINT-499301.

Structurei

3D structure databases

ProteinModelPortaliP48412.
SMRiP48412. Positions 86-190.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 3117Nuclear localization signalSequence analysisAdd
BLAST
Motifi58 – 7518Nuclear localization signalSequence analysisAdd
BLAST
Motifi284 – 30017Nuclear localization signalSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the RENT3 family.Curated

Phylogenomic databases

InParanoidiP48412.
KOiK14328.
OMAiLSPYVKK.
OrthoDBiEOG092C5M8L.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR005120. Nonsense_mediated_decay_UPF3.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PfamiPF03467. Smg4_UPF3. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.

Sequencei

Sequence statusi: Complete.

P48412-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVAGELKN SEGKKKGRGN RYHNKNRGKS KNETVDPKKN ENKVNNATNA
60 70 80 90 100
THNNSKGRRN NKKRNREYYN YKRKARLGKS TENEGFKLVI RLLPPNLTAD
110 120 130 140 150
EFFAILRDNN NDDGDKQDIQ GKLKYSDWCF FEGHYSSKVF KNSTYSRCNF
160 170 180 190 200
LFDNLSDLEK CANFIKTCKF IDNKDNITIP DMKLSPYVKK FTQTSKKDAA
210 220 230 240 250
LVGTIEEDEI FKTFMNSMKQ LNENDEYSFQ DFSVLKSLEK EFSKSIELEN
260 270 280 290 300
KIAERTERVL TELVGTGDKV KNKNKKKKNK NAKKKFKEEE ASAKIPKKKR
310 320 330 340 350
NRGKKKRENR EKSTISKTKN SNVVIIEEAG KEVLKQRKKK MLLQEKLKIS
360 370 380
NSSQPQSSSA QTQPSFQPKE NLFVPRVKIL HRDDTKK
Length:387
Mass (Da):44,924
Last modified:February 1, 1996 - v1
Checksum:i665A572D9A393133
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261N → D in AAT92769 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41153 Genomic DNA. Translation: AAC41671.1.
Z72857 Genomic DNA. Translation: CAA97074.1.
AY692750 Genomic DNA. Translation: AAT92769.1.
BK006941 Genomic DNA. Translation: DAA08166.1.
PIRiS62140.
RefSeqiNP_011586.1. NM_001181201.1.

Genome annotation databases

EnsemblFungiiYGR072W; YGR072W; YGR072W.
GeneIDi852963.
KEGGisce:YGR072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41153 Genomic DNA. Translation: AAC41671.1.
Z72857 Genomic DNA. Translation: CAA97074.1.
AY692750 Genomic DNA. Translation: AAT92769.1.
BK006941 Genomic DNA. Translation: DAA08166.1.
PIRiS62140.
RefSeqiNP_011586.1. NM_001181201.1.

3D structure databases

ProteinModelPortaliP48412.
SMRiP48412. Positions 86-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33315. 128 interactions.
DIPiDIP-2294N.
IntActiP48412. 2 interactions.
MINTiMINT-499301.

Proteomic databases

MaxQBiP48412.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR072W; YGR072W; YGR072W.
GeneIDi852963.
KEGGisce:YGR072W.

Organism-specific databases

EuPathDBiFungiDB:YGR072W.
SGDiS000003304. UPF3.

Phylogenomic databases

InParanoidiP48412.
KOiK14328.
OMAiLSPYVKK.
OrthoDBiEOG092C5M8L.

Enzyme and pathway databases

BioCyciYEAST:G3O-30785-MONOMER.

Miscellaneous databases

PROiP48412.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR005120. Nonsense_mediated_decay_UPF3.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PfamiPF03467. Smg4_UPF3. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUPF3_YEAST
AccessioniPrimary (citable) accession number: P48412
Secondary accession number(s): D6VUK5, E9P8W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.