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Protein

Chalcone synthase A

Gene

CHSA

Organism
Ipomoea purpurea (Common morning glory) (Pharbitis purpurea)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701PROSITE-ProRule annotation

GO - Molecular functioni

  1. naringenin-chalcone synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase A (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase A
Short name:
CHS-A
Gene namesi
Name:CHSA
OrganismiIpomoea purpurea (Common morning glory) (Pharbitis purpurea)
Taxonomic identifieri4121 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Chalcone synthase APRO_0000215992Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP48397.
SMRiP48397. Positions 15-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48397-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTTVTVLTD TWSHRAKRLE GDAKILAIGT ATPANWVDQT TYPDFYFRIT
60 70 80 90 100
NSEHLLEHKE KFRRICNKSK IMKRHLVITE ELLKKNPNLC TYNEASLNTR
110 120 130 140 150
QDILVSEVPK LGKDAAMKAI KEWGRPISEI THLVFCTTSG VDMPGADFQL
160 170 180 190 200
TKLLGLNSSV KRLMMYQQGC NAGAAMLRLA KDLAENNKGG RVLVVCSEVM
210 220 230 240 250
LSVFRGPSLQ QEDNLLAQCL FGDGAAAVIV GTEPRPGLET PLFELVSAAQ
260 270 280 290 300
TTIPDTDSYL KLQLREMGLT FHCSKAVPSL ITQNVEDCLV KAFEPFGISD
310 320 330 340 350
WNSIFWILHP GGNAILDGVE EKLGLEPEKL RASRDVLSQY GNLTSACVLF
360 370 380 390
ILDEVRKKSK KDEQMTTGEG LEWGVVFGFG PGLTIDTVVI RSVPIN
Length:396
Mass (Da):43,805
Last modified:July 15, 1999 - v2
Checksum:i2C06EA046DFCAC7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 196HRAKRL → RREKRN(PubMed:7724563)Curated
Sequence conflicti114 – 1141D → E in AAB02620. (PubMed:7724563)Curated
Sequence conflicti190 – 1901G → A in AAB02620. (PubMed:7724563)Curated
Sequence conflicti225 – 2251A → S in AAB02620. (PubMed:7724563)Curated
Sequence conflicti285 – 2851V → I in AAB02620. (PubMed:7724563)Curated
Sequence conflicti362 – 3632DE → QQ in AAB02620. (PubMed:7724563)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15946 Genomic DNA. Translation: AAB02620.1.
U84905 Genomic DNA. Translation: AAB62582.1.
U84907 Genomic DNA. Translation: AAB62584.1.
U84908 Genomic DNA. Translation: AAB62585.1.
U84909 Genomic DNA. Translation: AAB62586.1.
PIRiT10958.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15946 Genomic DNA. Translation: AAB02620.1.
U84905 Genomic DNA. Translation: AAB62582.1.
U84907 Genomic DNA. Translation: AAB62584.1.
U84908 Genomic DNA. Translation: AAB62585.1.
U84909 Genomic DNA. Translation: AAB62586.1.
PIRiT10958.

3D structure databases

ProteinModelPortaliP48397.
SMRiP48397. Positions 15-395.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolution of the chalcone synthase gene family in the genus Ipomoea."
    Durbin M.L., Learn G.H., Huttley G.A., Clegg M.T.
    Proc. Natl. Acad. Sci. U.S.A. 92:3338-3342(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide polymorphism in the chalcone synthase-A locus and evolution of the chalcone synthase multigene family of common morning glory (Ipomoea purpurea)."
    Huttley G.A., Durbin M.L., Glover D.E., Clegg M.T.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: IMEX16, IMEX19, IMEX22 and IMEX8.

Entry informationi

Entry nameiCHSA_IPOPU
AccessioniPrimary (citable) accession number: P48397
Secondary accession number(s): O04984
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: October 1, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.