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P48397 (CHSA_IPOPU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase A

EC=2.3.1.74
Alternative name(s):
Naringenin-chalcone synthase A
Short name=CHS-A
Gene names
Name:CHSA
OrganismIpomoea purpurea (Common morning glory) (Pharbitis purpurea)
Taxonomic identifier4121 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Chalcone synthase A
PRO_0000215992

Sites

Active site1701 By similarity

Experimental info

Sequence conflict14 – 196HRAKRL → RREKRN Ref.1
Sequence conflict1141D → E in AAB02620. Ref.1
Sequence conflict1901G → A in AAB02620. Ref.1
Sequence conflict2251A → S in AAB02620. Ref.1
Sequence conflict2851V → I in AAB02620. Ref.1
Sequence conflict362 – 3632DE → QQ in AAB02620. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48397 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 2C06EA046DFCAC7C

FASTA39643,805
        10         20         30         40         50         60 
MSTTVTVLTD TWSHRAKRLE GDAKILAIGT ATPANWVDQT TYPDFYFRIT NSEHLLEHKE 

        70         80         90        100        110        120 
KFRRICNKSK IMKRHLVITE ELLKKNPNLC TYNEASLNTR QDILVSEVPK LGKDAAMKAI 

       130        140        150        160        170        180 
KEWGRPISEI THLVFCTTSG VDMPGADFQL TKLLGLNSSV KRLMMYQQGC NAGAAMLRLA 

       190        200        210        220        230        240 
KDLAENNKGG RVLVVCSEVM LSVFRGPSLQ QEDNLLAQCL FGDGAAAVIV GTEPRPGLET 

       250        260        270        280        290        300 
PLFELVSAAQ TTIPDTDSYL KLQLREMGLT FHCSKAVPSL ITQNVEDCLV KAFEPFGISD 

       310        320        330        340        350        360 
WNSIFWILHP GGNAILDGVE EKLGLEPEKL RASRDVLSQY GNLTSACVLF ILDEVRKKSK 

       370        380        390 
KDEQMTTGEG LEWGVVFGFG PGLTIDTVVI RSVPIN 

« Hide

References

[1]"Evolution of the chalcone synthase gene family in the genus Ipomoea."
Durbin M.L., Learn G.H., Huttley G.A., Clegg M.T.
Proc. Natl. Acad. Sci. U.S.A. 92:3338-3342(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide polymorphism in the chalcone synthase-A locus and evolution of the chalcone synthase multigene family of common morning glory (Ipomoea purpurea)."
Huttley G.A., Durbin M.L., Glover D.E., Clegg M.T.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IMEX16, IMEX19, IMEX22 and IMEX8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15946 Genomic DNA. Translation: AAB02620.1.
U84905 Genomic DNA. Translation: AAB62582.1.
U84907 Genomic DNA. Translation: AAB62584.1.
U84908 Genomic DNA. Translation: AAB62585.1.
U84909 Genomic DNA. Translation: AAB62586.1.
PIRT10958.

3D structure databases

ProteinModelPortalP48397.
SMRP48397. Positions 15-395.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHSA_IPOPU
AccessionPrimary (citable) accession number: P48397
Secondary accession number(s): O04984
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: February 19, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways