P48390 (CHS1_GERHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase 1
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase 1

Gene names

Name:

CHS1

Organism

Gerbera hybrida (Daisy)

Taxonomic identifier

18101 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Mutisioideae › Mutisieae › Gerbera

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Chalcone synthase 1		PRO_0000215977
Sites
	Active site	167	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48390 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A5D095314D6F9A1D	FASTA	398	43,494
	10 20 30 40 50 60 MASSVDMKAI RDAQRAEGPA TILAIGTATP ANCVYQADYP DYYFRITKSE HMVDLKEKFK 70 80 90 100 110 120 RMCDKSMIRK RYMHITEEYL KQNPNMCAYM APSLDVRQDL VVVEVPKLGK EAAMKAIKEW 130 140 150 160 170 180 GHPKSKITHL IFCTTSGVDM PGADYQLTKL LGLRPSVKRF MMYQQGCFAG GTVLRLAKDL 190 200 210 220 230 240 AENNKGARVL VVCSEITAVT FRGPNDTHLD SLVGQALFGD GAAAVIVGSD PDLTTERPLF 250 260 270 280 290 300 EMVSAAQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIEKALTTAF SPLGINDWNS 310 320 330 340 350 360 IFWIAHPGGP AILDQVELKL GLKEEKLRAT RHVLSEYGNM SSACVLFIID EMRKKSSENG 370 380 390 AGTTGEGLEW GVLFGFGPGL TVETVVLHSV PTTVTVAV

References

[1]

"Chalcone synthase-like genes active during corolla development are differentially expressed and encode enzymes with different catalytic properties in Gerbera hybrida (Asteraceae)." Helariutta Y., Elomaa P., Kotilainen M., Griesbach R.J., Schroeder J., Teeri T.H. Plant Mol. Biol. 28:47-60(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Corolla.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38096 mRNA. Translation: CAA86218.1.
PIR	S56699.
3D structure databases
ProteinModelPortal	P48390.
SMR	P48390. Positions 10-390.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00154.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS1_GERHY

Accession

Primary (citable) accession number: P48390

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program