P48385 (CHSY_CALCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase

Gene names

Name:

CHS

Organism

Callistephus chinensis (China aster) (Callistemma chinense)

Taxonomic identifier

13379 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Astereae › Australasian lineages › Callistephus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Chalcone synthase		PRO_0000215959
Sites
	Active site	167	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48385 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 8B7D4E11B4FAFFC3	FASTA	398	43,541
	10 20 30 40 50 60 MASTIDIAAI REAQRRQGPA TILAIGTATP SNCVYQADYP DYYFRITKSE HMVDLKEKFK 70 80 90 100 110 120 RMCDKSMIRK RYMHLTEEYL KENPSLCEYM APSLDARQDV VVVEVPKLGK EAATKAIKEW 130 140 150 160 170 180 GQPKSKITHL IFCTTSGVDM PGADYQLTKL LGLRPSVKRF MMYQQGCFAG GTVLRLAKDL 190 200 210 220 230 240 AENNKGARVL VVCSEITAVT FRGPNDTHLD SLVGQALFGD GAAAVIVGAD PDLTTERPLF 250 260 270 280 290 300 EMISAAQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIEKALTQAF SPLGITDWNS 310 320 330 340 350 360 IFWIAHPGGP AILDQVELKL GLKEEKMRAT RHVLSEYGNM SSACVLFIID EMRKKSAEDG 370 380 390 AATTGEGLDW GVLFGFGPGL TVETVVLHSL PTTMAIAT

References

[1]	Henkel J., Wassenegger M., Sommer H., Forkmann G. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: L 01. Tissue: Petal.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z67988 mRNA. Translation: CAA91930.1.
3D structure databases
ProteinModelPortal	P48385.
SMR	P48385. Positions 7-390.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00154.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHSY_CALCH

Accession

Primary (citable) accession number: P48385

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 15, 1998
Last modified:	March 6, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program