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Protein

Protein sak1

Gene

sak1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates cyclic AMP-dependent protein kinase-mediated exit from the mitotic cell cycle.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi101 – 17676RFX-type winged-helixPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: GOC
  • positive regulation of transcription from RNA polymerase II promoter Source: GOC
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sak1
Gene namesi
Name:sak1
ORF Names:SPAC3G9.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3G9.14.
PomBaseiSPAC3G9.14. sak1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 766766Protein sak1PRO_0000215293Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei223 – 2231Phosphoserine1 Publication
Modified residuei224 – 2241Phosphoserine1 Publication
Modified residuei227 – 2271Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48383.

PTM databases

iPTMnetiP48383.
SwissPalmiP48383.

Interactioni

Protein-protein interaction databases

BioGridi279843. 9 interactions.
MINTiMINT-4690483.

Structurei

3D structure databases

ProteinModelPortaliP48383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi726 – 7327Poly-Gln

Sequence similaritiesi

Belongs to the RFX family.PROSITE-ProRule annotation
Contains 1 RFX-type winged-helix DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP48383.
KOiK09175.
OMAiYFSAFHG.
OrthoDBiEOG741ZBV.
PhylomeDBiP48383.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003150. DNA-bd_RFX.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF02257. RFX_DNA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51526. RFX_DBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPSDLPGQI PLSRSDMNVQ DQLDPVQRFD THFMLPQEEN FLNRPSITSE
60 70 80 90 100
SAHPRGSDLE QETELKRLAL EHEHYSLESL AEKLRMDHVS ANSEKFRQVF
110 120 130 140 150
GICWLKRACE EQQDAAVQRN QIYAHYVEIC NSLHIKPLNS ASFGKLVRLL
160 170 180 190 200
FPSIKTRRLG MRGHSKYHYC GIKLRGQDSF RRLRTFSDSS LSPVSCSSFP
210 220 230 240 250
KPIPNHFEND VSSIQNTNQR VESSPASVNA AAIVRKSAVT PSSDPYNSPP
260 270 280 290 300
PSIPLLGSQT NLQLAPSFAA PQAHPLPSHL SQSNVPPQLS HSSVPSPAPP
310 320 330 340 350
RSVSQPTYFS QPMPQFSSSF VPGTSSIVPT LHPASAQEDF NLQHSLFFKL
360 370 380 390 400
KLKFLPPHKL PWIPSLDVSS FSLPPIDYYL NGPYDNVEAK SALMNIYSSH
410 420 430 440 450
CITLIESVRY MHLKQFLSEI SNFPNSLSPS LLALLSSPYF TKWIERSDTV
460 470 480 490 500
MYREILKLLF PMTLQVVPPP VLVLLRHLAE NLVNHISSIY ASHSSCLLQV
510 520 530 540 550
KSETAAIFSN LLSRLLRVND TAHAAARFLA NPADRHLICN DWERFVSTRF
560 570 580 590 600
IVHRELMCND KEAVAALDEW YSILSTCSNP SELLDPLKDK HEASDTSMNR
610 620 630 640 650
VELRQIDGVL DRMADFFLEL PSRFPSCSPR MFLLCLGALQ TSVLREITVS
660 670 680 690 700
GGEAFGALWV IRCWVDEYMT WVAEIGGYLD DSYDELEQHH ANFHNKAGIS
710 720 730 740 750
QSNIPPHLQE HRQSQQHFQQ DIEALQSQQQ QQATKNSLME AAYQNAQKQK
760
EDDYISIVFD TNGACS
Length:766
Mass (Da):86,405
Last modified:December 15, 1998 - v2
Checksum:i1792B52FF8FA605E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti728 – 76639QQQQQ…NGACS → TTTTTSH in AAA67937 (PubMed:7862141).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19978 Genomic DNA. Translation: AAA67937.1.
CU329670 Genomic DNA. Translation: CAA15923.1.
PIRiT11650.
T52535.
RefSeqiNP_594086.1. NM_001019499.2.

Genome annotation databases

EnsemblFungiiSPAC3G9.14.1; SPAC3G9.14.1:pep; SPAC3G9.14.
GeneIDi2543421.
KEGGispo:SPAC3G9.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19978 Genomic DNA. Translation: AAA67937.1.
CU329670 Genomic DNA. Translation: CAA15923.1.
PIRiT11650.
T52535.
RefSeqiNP_594086.1. NM_001019499.2.

3D structure databases

ProteinModelPortaliP48383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279843. 9 interactions.
MINTiMINT-4690483.

PTM databases

iPTMnetiP48383.
SwissPalmiP48383.

Proteomic databases

MaxQBiP48383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC3G9.14.1; SPAC3G9.14.1:pep; SPAC3G9.14.
GeneIDi2543421.
KEGGispo:SPAC3G9.14.

Organism-specific databases

EuPathDBiFungiDB:SPAC3G9.14.
PomBaseiSPAC3G9.14. sak1.

Phylogenomic databases

InParanoidiP48383.
KOiK09175.
OMAiYFSAFHG.
OrthoDBiEOG741ZBV.
PhylomeDBiP48383.

Miscellaneous databases

NextBioi20804436.
PROiP48383.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003150. DNA-bd_RFX.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF02257. RFX_DNA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51526. RFX_DBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sak1+ gene of Schizosaccharomyces pombe encodes an RFX family DNA-binding protein that positively regulates cyclic AMP-dependent protein kinase-mediated exit from the mitotic cell cycle."
    Wu S.Y., McLeod M.
    Mol. Cell. Biol. 15:1479-1488(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223; SER-224 AND SER-227, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSAK1_SCHPO
AccessioniPrimary (citable) accession number: P48383
Secondary accession number(s): O42876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 15, 1998
Last modified: April 13, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.