ID   RFX1_MOUSE              Reviewed;         963 AA.
AC   P48377; Q6PGH8;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   24-JAN-2024, entry version 156.
DE   RecName: Full=MHC class II regulatory factor RFX1;
DE   AltName: Full=Regulatory factor X 1;
DE   AltName: Full=Transcription factor RFX1;
GN   Name=Rfx1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=BALB/cJ; TISSUE=Spleen;
RX   PubMed=8289803; DOI=10.1128/mcb.14.2.1230-1244.1994;
RA   Reith W., Ucla C., Barras E., Gaud A., Durand B., Herrero-Sanchez C.,
RA   Kobr M., Mach B.;
RT   "RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel
RT   family of homodimeric and heterodimeric DNA-binding proteins.";
RL   Mol. Cell. Biol. 14:1230-1244(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   SUBUNIT.
RX   PubMed=15229132; DOI=10.1095/biolreprod.104.032268;
RA   Horvath G.C., Kistler W.S., Kistler M.K.;
RT   "RFX2 is a potential transcriptional regulatory factor for histone H1t and
RT   other genes expressed during the meiotic phase of spermatogenesis.";
RL   Biol. Reprod. 71:1551-1559(2004).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Lung;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Regulatory factor essential for MHC class II genes
CC       expression. Binds to the X boxes of MHC class II genes (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; binds DNA as a homodimer (By similarity).
CC       Heterodimer; heterodimerizes with RFX2 and RFX3 (PubMed:15229132).
CC       {ECO:0000250|UniProtKB:P22670, ECO:0000269|PubMed:15229132}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00858}.
CC   -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00858}.
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DR   EMBL; X76088; CAA53702.1; -; mRNA.
DR   EMBL; CH466525; EDL10930.1; -; Genomic_DNA.
DR   EMBL; BC057018; AAH57018.1; -; mRNA.
DR   CCDS; CCDS22468.1; -.
DR   PIR; A55926; A55926.
DR   RefSeq; NP_033081.3; NM_009055.4.
DR   AlphaFoldDB; P48377; -.
DR   SMR; P48377; -.
DR   BioGRID; 202872; 4.
DR   STRING; 10090.ENSMUSP00000005600; -.
DR   GlyGen; P48377; 9 sites, 1 O-linked glycan (9 sites).
DR   iPTMnet; P48377; -.
DR   PhosphoSitePlus; P48377; -.
DR   SwissPalm; P48377; -.
DR   EPD; P48377; -.
DR   jPOST; P48377; -.
DR   MaxQB; P48377; -.
DR   PaxDb; 10090-ENSMUSP00000005600; -.
DR   PeptideAtlas; P48377; -.
DR   ProteomicsDB; 255188; -.
DR   Pumba; P48377; -.
DR   Antibodypedia; 26599; 200 antibodies from 26 providers.
DR   DNASU; 19724; -.
DR   Ensembl; ENSMUST00000005600.6; ENSMUSP00000005600.6; ENSMUSG00000031706.8.
DR   GeneID; 19724; -.
DR   KEGG; mmu:19724; -.
DR   UCSC; uc009mlv.2; mouse.
DR   AGR; MGI:105982; -.
DR   CTD; 5989; -.
DR   MGI; MGI:105982; Rfx1.
DR   VEuPathDB; HostDB:ENSMUSG00000031706; -.
DR   eggNOG; KOG3712; Eukaryota.
DR   GeneTree; ENSGT01050000244879; -.
DR   HOGENOM; CLU_010393_1_0_1; -.
DR   InParanoid; P48377; -.
DR   OMA; CCLEDER; -.
DR   OrthoDB; 2915941at2759; -.
DR   PhylomeDB; P48377; -.
DR   TreeFam; TF321340; -.
DR   BioGRID-ORCS; 19724; 3 hits in 77 CRISPR screens.
DR   ChiTaRS; Rfx1; mouse.
DR   PRO; PR:P48377; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; P48377; Protein.
DR   Bgee; ENSMUSG00000031706; Expressed in ascending aorta and 219 other cell types or tissues.
DR   ExpressionAtlas; P48377; baseline and differential.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IDA:MGI.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR003150; DNA-bd_RFX.
DR   InterPro; IPR039779; RFX-like.
DR   InterPro; IPR007668; RFX1_trans_act.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12619:SF23; MHC CLASS II REGULATORY FACTOR RFX1; 1.
DR   PANTHER; PTHR12619; RFX TRANSCRIPTION FACTOR FAMILY; 1.
DR   Pfam; PF04589; RFX1_trans_act; 1.
DR   Pfam; PF02257; RFX_DNA_binding; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS51526; RFX_DBD; 1.
DR   Genevisible; P48377; MM.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..963
FT                   /note="MHC class II regulatory factor RFX1"
FT                   /id="PRO_0000215287"
FT   DNA_BIND        423..498
FT                   /note="RFX-type winged-helix"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT   REGION          1..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          105..126
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          174..218
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          361..392
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          899..948
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        17..43
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        44..66
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        905..922
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         54
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P22670"
FT   MOD_RES         962
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P22670"
FT   MOD_RES         963
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P22670"
FT   CONFLICT        169..170
FT                   /note="QH -> HD (in Ref. 1; CAA53702)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   963 AA;  103707 MW;  C868182B206BA9F0 CRC64;
     MATQSYVTEL QAAPQASQPP QAPPQALPQP PPPAAPQPPA AATPQPQYVT ELQSPQPQTQ
     PPGSQKQYVA ELPAAPAPSQ PATPAPSPVA QQYIVVTVSE GAMRASETVS EASPSSTASQ
     TGVPTQVVQQ VQGTQQRLLV QASVQAKPGH VSPLQLTNIQ VPQQAIPTQH LVVQSPAPGT
     KSGQVSLTVH SAQQVHSAPE RSPVQANNST SKTAGTPAAT VQQLQVHSVQ QSVPVTQERS
     VVQATPQTKA GPVQQLTVQG LQPVHVAQEV QQLPQVPVPH VYSSQVQYVE GGDASYTASA
     IRSSTYQYPE TPIYTQTAGT SYYEASGTAA QVSTPATSQT VASSGSVPMY VSGSPIVASS
     SSSEAGASNS SVGAGGNGGG GSSGGGSGGS SGSGAGTYVI QGGYMLGNAS QSYSHTTRAS
     PATVQWLLDN YETAEGVSLP RSTLYCHYLL HCQEQKLEPV NAASFGKLIR SVFMGLRTRR
     LGTRGNSKYH YYGLRIKASS PLLRLMEDQQ HMAMRGQPFS QKQRLKPIQK MEGVANGVAV
     GQQSTGLSDI SAQVQQYQQF LDASRSLPDF AELDLQGKVL PEGVGPGDIK AFQVLYREHC
     EAIVDVMVNL QFTLVETLWK TFWRYNLSQP SEAPPLAVHD EAEKRLPRAS LVLLSKFQPV
     LQWTKHCDNV LYQGLVEILI PDVLRPIPSA LTQAIRNFAK SLESWLTHAM VNIPEEMLRV
     KVAAAGAFAQ TLRRYTSLNH LAQAARAVLQ NTAQINQMLS DLNRVDFANV QEQASWVCRC
     EDRVVQRLEQ DFKVTLQQQN SLEQWAAWLD GVVSQVLKPY QGSSGFPKAA KLFLLKWSFY
     SSMVIRDLTL RSAASFGSFH LIRLLYDEYM YYLIEHRVAQ AKGETPIAVM GEFANLATSL
     NPLDPDKDEE EEEEEESEDE LPQDISLAAG SESPALGPEA LEPPAKLART DTRGLFVQAL
     PSS
//