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Protein

5-demethoxyubiquinone hydroxylase, mitochondrial

Gene

clk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 2 iron ions per subunit.UniRule annotation

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron 1UniRule annotation
Metal bindingi60 – 601Iron 1UniRule annotation
Metal bindingi60 – 601Iron 2UniRule annotation
Metal bindingi63 – 631Iron 1UniRule annotation
Metal bindingi112 – 1121Iron 2UniRule annotation
Metal bindingi148 – 1481Iron 1UniRule annotation
Metal bindingi148 – 1481Iron 2UniRule annotation
Metal bindingi151 – 1511Iron 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • adult behavior Source: WormBase
  • cellular respiration Source: WormBase
  • determination of adult lifespan Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of protein phosphorylation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: WormBase
  • oxidative phosphorylation Source: WormBase
  • positive regulation of behavior Source: WormBase
  • positive regulation of developmental process Source: WormBase
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of growth rate Source: WormBase
  • positive regulation of nematode larval development Source: UniProtKB
  • positive regulation of protein oxidation Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  • regulation of reactive oxygen species metabolic process Source: WormBase
  • reproduction Source: WormBase
  • response to drug Source: WormBase
  • ubiquinone biosynthetic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13880.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
5-demethoxyubiquinone hydroxylase, mitochondrialUniRule annotation (EC:1.14.13.-UniRule annotation)
Short name:
DMQ hydroxylaseUniRule annotation
Alternative name(s):
Clock abnormal protein 1
Short name:
Protein clk-1
Ubiquinone biosynthesis monooxygenase COQ7UniRule annotation
Gene namesi
Name:clk-1UniRule annotation
ORF Names:ZC395.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZC395.2; CE01438; WBGene00000536; clk-1.

Subcellular locationi

  • Mitochondrion inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Matrix side UniRule annotation

GO - Cellular componenti

  • extrinsic component of mitochondrial inner membrane Source: UniProtKB-HAMAP
  • mitochondrion Source: WormBase
  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Worms show deregulation timing of a wide range of physiological processes. This leads to an average lengthening of the worm's early cell cycles, the embryonic and postembryonic development, and the period of rhythmic adult behaviors.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi148 – 1481E → K in E2519. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 88MitochondrionSequence analysis
Chaini9 – 1871795-demethoxyubiquinone hydroxylase, mitochondrialPRO_0000079250Add
BLAST

Proteomic databases

EPDiP48376.
PaxDbiP48376.

Expressioni

Gene expression databases

BgeeiWBGene00000536.

Interactioni

Subunit structurei

Component of a multi-subunit COQ enzyme complex.UniRule annotation

Protein-protein interaction databases

STRINGi6239.ZC395.2.2.

Structurei

3D structure databases

ProteinModelPortaliP48376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati28 – 99721Add
BLAST
Repeati100 – 187882Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 1871602 X approximate tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the COQ7 family.UniRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG4061. Eukaryota.
COG2941. LUCA.
GeneTreeiENSGT00390000014520.
HOGENOMiHOG000184972.
InParanoidiP48376.
KOiK06134.
OMAiEGHEQVH.
OrthoDBiEOG091G0N8Z.
PhylomeDBiP48376.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
HAMAPiMF_01658. COQ7. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR011566. Ubq_synth_Coq7.
[Graphical view]
PANTHERiPTHR11237. PTHR11237. 1 hit.
PfamiPF03232. COQ7. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48376-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRVITRGAH TAASRQALIE KIIRVDHAGE LGADRIYAGQ LAVLQGSSVG
60 70 80 90 100
SVIKKMWDEE KEHLDTMERL AAKHNVPHTV FSPVFSVAAY ALGVGSALLG
110 120 130 140 150
KEGAMACTIA VEELIGQHYN DQLKELLADD PETHKELLKI LTRLRDEELH
160 170 180
HHDTGVEHDG MKAPAYSALK WIIQTGCKGA IAIAEKI
Length:187
Mass (Da):20,466
Last modified:February 1, 1996 - v1
Checksum:i4EFF688778CE4151
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080775 Genomic DNA. Translation: CCD66658.1.
PIRiT27545.
RefSeqiNP_498128.1. NM_065727.3.
UniGeneiCel.10672.

Genome annotation databases

EnsemblMetazoaiZC395.2; ZC395.2; WBGene00000536.
GeneIDi175729.
KEGGicel:CELE_ZC395.2.
UCSCiZC395.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080775 Genomic DNA. Translation: CCD66658.1.
PIRiT27545.
RefSeqiNP_498128.1. NM_065727.3.
UniGeneiCel.10672.

3D structure databases

ProteinModelPortaliP48376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZC395.2.2.

Proteomic databases

EPDiP48376.
PaxDbiP48376.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZC395.2; ZC395.2; WBGene00000536.
GeneIDi175729.
KEGGicel:CELE_ZC395.2.
UCSCiZC395.2. c. elegans.

Organism-specific databases

CTDi175729.
WormBaseiZC395.2; CE01438; WBGene00000536; clk-1.

Phylogenomic databases

eggNOGiKOG4061. Eukaryota.
COG2941. LUCA.
GeneTreeiENSGT00390000014520.
HOGENOMiHOG000184972.
InParanoidiP48376.
KOiK06134.
OMAiEGHEQVH.
OrthoDBiEOG091G0N8Z.
PhylomeDBiP48376.

Enzyme and pathway databases

UniPathwayiUPA00232.
BioCyciMetaCyc:MONOMER-13880.

Miscellaneous databases

PROiP48376.

Gene expression databases

BgeeiWBGene00000536.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
HAMAPiMF_01658. COQ7. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR011566. Ubq_synth_Coq7.
[Graphical view]
PANTHERiPTHR11237. PTHR11237. 1 hit.
PfamiPF03232. COQ7. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOQ7_CAEEL
AccessioniPrimary (citable) accession number: P48376
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.