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Reviewed, UniProtKB/Swiss-Prot P48372 (GYRA_PSEAE)

Last modified November 3, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA gyrase subunit A
    EC=5.99.1.3
Gene names
Name: gyrA
Ordered Locus Names: PA3168
OrganismPseudomonas aeruginosa [Complete proteome] [HAMAP]
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length923 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Subunit structure

Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.

Sequence similarities

Belongs to the topoisomerase gyrA/parC subunit family.

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Ontologies

Keywords
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 923923DNA gyrase subunit A
PRO_0000145245

Sites

Active site1221O-(5'-phospho-DNA)-tyrosine intermediate By similarity

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Sequences

Sequence LengthMass (Da)Tools
P48372-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 0025DFE08CDD1BEC

FASTA923101,135
        10         20         30         40         50         60 
MGELAKEILP VNIEDELKQS YLDYAMSVIV GRALPDARDG LKPVHRRVLY AMSELGNDWN 

        70         80         90        100        110        120 
KPYKKSARVV GDVIGKYHPH GDTAVYDTIV RMAQPFSLRY MLVDGQGNFG SVDGDNAAAM 

       130        140        150        160        170        180 
RYTEVRMAKL AHELLADLEK ETVDWVPNYD GTEQIPAVMP TKIPNLLVNG SSGIAVGMAT 

       190        200        210        220        230        240 
NIPPHNLGEV IDGCLALMDN PDLTVDELMQ YIPGPDFPTA GIINGRAGII EAYRTGRGRI 

       250        260        270        280        290        300 
YIRARAVVEE MEKGGGREQI IITELPYQLN KARLIEKIAE LVKEKKIEGI SELRDESDKD 

       310        320        330        340        350        360 
GMRVVIELRR GEVGEVVLNN LYAQTQLQSV FGINVVALVD GQPRTLNLKD MLEVFVRHRR 

       370        380        390        400        410        420 
EVVTRRTVYE LRKARERGHI LEGQAVALSN IDPVIELIKS SPTPAEAKER LIATAWESSA 

       430        440        450        460        470        480 
VEAMVERAGA DACRPEDLDP QYGLRDGKYY LSPEQAQAIL ELRLHRLTGL EHEKLLSEYQ 

       490        500        510        520        530        540 
EILNLIGELI RILTNPARLM EVIREELEAV KAEFGDARRT EIVASQVDLT IADLITEEDR 

       550        560        570        580        590        600 
VVTISHGGYA KSQPLAAYQA QRRGGKGKSA TGMKDEDYIE HLLVANSHAT LLLFSSKGKV 

       610        620        630        640        650        660 
YWLRTFEIPE ASRTARGRPL VNLLPLDEGE RITAMLQIDL EALQQNGGAD DDLDEAEGAV 

       670        680        690        700        710        720 
LEGEVVEAAE VEEVEGETAE LVAEPTGAYI FMATAFGTVK KTPLVQFSRP RSSGLIALKL 

       730        740        750        760        770        780 
EEGDTLIAAA ITDGAKEVML FSSAGKVIRF AESVVRIMGR NARGVRGMRL GKGQQLISML 

       790        800        810        820        830        840 
IPESGAQILT ASERGFGKRT PLSKFPRRGR GGQGVIAMVT NERNGALIAA VQVQEGEEIM 

       850        860        870        880        890        900 
LISDQGTLVR TRVDEVSLSG RNTQGVTLIK LASDEVLVGL ERVQEPSGGD DEDLPEGEEA 

       910        920 
AESLGESAES ESEPAAEAEG NEE

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequence of Pseudomonas aeruginosa DNA gyrase gyrA gene from strain PAO1 and quinolone-resistant clinical isolates."
Kureishi A., Diver J.M., Beckthold B., Schollaardt T., Bryan L.E.
Antimicrob. Agents Chemother. 38:1944-1952(1994) [PubMed: 7811002] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

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Cross-references

Sequence databases

L29417 Genomic DNA. Translation: AAA68625.1.
AE004091 Genomic DNA. Translation: AAG06556.1.
PIRH83248.
RefSeqNP_251858.1.

3D structure databases

HSSPHSSP built from PDB template 1AB4 based on UniProtKB P09097.
SMRP48372. Positions 30-522.
ModBaseSearch...

Genome annotation databases

GeneID882800.
GenomeReviewsGene locus PA3168 in contig AE004091_GR.
KEGGpae:PA3168.

Organism-specific databases

PseudoCAPPA3168.
CMRSearch...

Phylogenomic databases

HOGENOMP48372.
OMAELGNDWN.

Enzyme and pathway databases

BioCycPAER208964:PA3168-MON.
BRENDA5.99.1.3. 354.

Family and domain databases

InterProIPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
[Graphical view]
Gene3DG3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
ProDomPD000742. DNA_topoisoIV. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGYRA_PSEAE
AccessionPrimary (citable) accession number: P48372
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents