P48371 (GYRA_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	916 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 916	916	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145244
Sites
	Active site	130	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48371 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 6392550101C261CF	FASTA	916	101,530
	10 20 30 40 50 60 MTDATIRHDH KFALETLPVS LEDEMRKSYL DYAMSVIVGR ALPDVRDGLK PVHRRVLYAM 70 80 90 100 110 120 HELKNNWNAA YKKSARIVGD VIGKYHPHGD SAVYDTIVRM AQNFAMRYVL IDGQGNFGSV 130 140 150 160 170 180 DGLAAAAMRY TEIRMAKISH EMLADIEEET VNFGPNYDGS EHEPLVLPTR FPTLLVNGSS 190 200 210 220 230 240 GIAVGMATNI PPHNLTDTIN ACLRLLDEPK TEIDELIDII QAPDFPTGAT IYGLGGVREG 250 260 270 280 290 300 YKTGRGRVVM RGKTHIEPIG KNGERERIVI DEIPYQVNKA KLVEKIGDLV REKTLEGISE 310 320 330 340 350 360 LRDESDKSGM RVVIELKRNE NAEVVLNQLY KLTPLQDSFG INMVVLVDGQ PRLLNLKQIL 370 380 390 400 410 420 SEFLRHRREV VTRRTLFRLK KARHEGHIAE RKAVALSNID EIIKLIKESP NAAEAKEKLL 430 440 450 460 470 480 ARPWASSLVE EMLTRSGLDL EMMRPEGLVA NIGLKKQGYY LSEIQADAIL RMSLRNLTGL 490 500 510 520 530 540 DQKEIIESYK NLMGKIIDFV DILSKPERIT QIIRDELEEI KTNYGDERRS EINPFGGDIA 550 560 570 580 590 600 DEDLIPQREM VVTLTHGGYI KTQPTTDYQA QRRGGRGKQA AATKDEDFIE TLFVANTHDY 610 620 630 640 650 660 LMCFTNLGKC HWIKVYKLPE GGRNSRGRPI NNVIQLEEGE KVSAILAVRE FPEDQYVFFA 670 680 690 700 710 720 TAQGMVKKVQ LSAFKNVRAQ GIKAIALKEG DYLVGAAQTG GADDIMLFSN LGKAIRFNEY 730 740 750 760 770 780 WEKSGNDEAE DADIETEISD DLEDETADNE NTLPSGKNGV RPSGRGSGGL RGMRLPADGK 790 800 810 820 830 840 IVSLITFAPE TEESGLQVLT ATANGYGKRT PIADYSRKNK GGQGSIAINT GERNGDLVAA 850 860 870 880 890 900 TLVGETDDLM LITSGGVLIR TKVEQIRETG RAAAGVKLIN LDEGETLVSL ERVAEDESEL 910 SGASVISNVT EPEAEN

References

[1]	"Neisseria gonorrhoeae acquires mutations in analogous regions of gyrA and parC in fluoroquinolone-resistant isolates." Belland R.J., Morrison S.G., Ison C., Huang W.M. Mol. Microbiol. 14:371-380(1994) [PubMed: 7830580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MS11.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U08817 Genomic DNA. Translation: AAA82128.1.
PIR	S60779.
3D structure databases
ProteinModelPortal	P48371.
SMR	P48371. Positions 38-533.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_NEIGO

Accession

Primary (citable) accession number: P48371

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program