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P48370 (GYRA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A
EC=5.99.1.3
Gene names
Name:gyrA
Ordered Locus Names:HP_0701
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897

Subunit structure

Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.

Subcellular location

Cytoplasm Potential HAMAP MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 827827DNA gyrase subunit A HAMAP MF_01897
PRO_0000145236

Sites

Active site1261O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Sequence conflict4 – 52NS → RL in AAA74376. Ref.1
Sequence conflict1911M → I in AAA74376. Ref.1
Sequence conflict1991V → A in AAA74376. Ref.1
Sequence conflict2081G → E in AAA74376. Ref.1
Sequence conflict3541H → R in AAA74376. Ref.1
Sequence conflict3971R → Q in AAA74376. Ref.1
Sequence conflict4031Q → P in AAA74376. Ref.1
Sequence conflict4951N → S in AAA74376. Ref.1
Sequence conflict5241A → V in AAA74376. Ref.1
Sequence conflict6101D → N in AAA74376. Ref.1
Sequence conflict6321E → G in AAA74376. Ref.1
Sequence conflict6791E → D in AAA74376. Ref.1
Sequence conflict6831I → M in AAA74376. Ref.1
Sequence conflict686 – 6872TT → NA in AAA74376. Ref.1
Sequence conflict6941K → R in AAA74376. Ref.1
Sequence conflict7331G → E in AAA74376. Ref.1
Sequence conflict8191T → N Ref.1
Sequence conflict821 – 8222SA → PT Ref.1
Sequence conflict8241Missing Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48370 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 3631E6AFCF4279F2

FASTA82792,500
        10         20         30         40         50         60 
MQDNSVNETK NIVEVGIDSS IEESYLAYSM SVIIGRALPD ARDGLKPVHR RILYAMHELG 

        70         80         90        100        110        120 
LTSKVAYKKS ARIVGDVIGK YHPHGDNAVY DALVRMAQDF SMRLELVDGQ GNFGSIDGDN 

       130        140        150        160        170        180 
AAAMRYTEAR MTKASEEILR DIDKDTIDFV PNYDDTLKEP DILPSRLPNL LVNGANGIAV 

       190        200        210        220        230        240 
GMATSIPPHR MDEIIDALVH VLENPNAGLD EILEFVKGPD FPTGGIIYGK AGIIEAYKTG 

       250        260        270        280        290        300 
RGRVKVRAKV HVEKTKNKEI IVLDEMPFQT NKAKLVEQIS DLAREKQIEG ISEVRDESDR 

       310        320        330        340        350        360 
EGIRVVIELK RDAMSEIVLN HLYKLTTMET TFSIILLAIY NKEPKIFTLL ELLHLFLNHR 

       370        380        390        400        410        420 
KTIIIRRTIF ELEKAKARAH ILEGYLIALD NIDEIVRLIK TSQSPEAAKN ALMERFTLSE 

       430        440        450        460        470        480 
IQSKAILEMR LQRLTGLERD KIKEEYQNLL ELIDDLNGIL KSEDRLNGVV KTELLEVKEQ 

       490        500        510        520        530        540 
FSSPRRTEIQ ESYENIDIED LIANEPMVVS MSYKGYVKRV DLKAYEKQNR GGKGKLSGST 

       550        560        570        580        590        600 
YEDDFIENFF VANTHDILLF ITNKGQLYHL KVYKIPEASR IAMGKAIVNL ISLAPDEKIM 

       610        620        630        640        650        660 
ATLSTKDFSD ERSLAFFTKN GVVKRTNLSE FESNRSCGIR AIVLDEGDEL VSAKVVDKNA 

       670        680        690        700        710        720 
KHLLIASHLG IFIKFPLEEV REIGRTTRGV IGIKLNENDF VVGAVVISDD GNKLLSVSEN 

       730        740        750        760        770        780 
GLGKQTLAEA YRGQSRGGKG VIGMKLTQKT GNLVGVISVD DENLDLMILT ASAKMIRVSI 

       790        800        810        820 
KDIRETGRNA SGVKLINTAD KVMYVNSCPK EEEPENLETS SAQNLFE

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the gyrA gene and characterization of ciprofloxacin-resistant mutants of Helicobacter pylori."
Moore R.A., Beckthold B., Wong S., Kureishi A., Bryan L.E.
Antimicrob. Agents Chemother. 39:107-111(1995) [PubMed: 7695290] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: UC946.
[2]"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. expand/collapse author list , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.
Nature 388:539-547(1997) [PubMed: 9252185] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L29481 Genomic DNA. Translation: AAA74376.1.
AE000511 Genomic DNA. Translation: AAD07753.1.
PIRE64607.
RefSeqNP_207495.1. NC_000915.1.

3D structure databases

ProteinModelPortalP48370.
SMRP48370. Positions 34-490.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-3353N.
MINTMINT-185350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID898803.
GenomeReviewsGene locus HP_0701 in contig AE000511_GR.
KEGGhpy:HP0701.
NMPDRfig|85962.1.peg.693.
PATRIC20592689. VBIHelPyl33062_0733.
TIGRHP_0701.

Phylogenomic databases

HOGENOMHBG635183.
OMATSIPPHR.
ProtClustDBPRK05560.

Family and domain databases

HAMAPMF_01897. GyrA.
[Tree]
InterProIPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013760. Topo_IIA_cen.
[Graphical view]
Gene3DG3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KOK02469.
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01063. GyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGYRA_HELPY
AccessionPrimary (citable) accession number: P48370
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents