P48369 (GYRA_AERSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Aeromonas salmonicida

Taxonomic identifier

645 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	922 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 922	922	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145218
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48369 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 8894965DC4217077	FASTA	922	101,334
	10 20 30 40 50 60 MSDLAREITP INIEEELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLF AMNELGNDWN 70 80 90 100 110 120 KPYKKSARVV GDVIGKYHPH GDSAVYDTIV RLAQDFSMRY MLVDGQGNFG SVDGDSAAAM 130 140 150 160 170 180 RYTEVRMARI SHELLADLDK ETVDWVPNYD GTEMIPAVMP TKVPNLLVNG SSGIAVGMAW 190 200 210 220 230 240 NIPPHNLTEI VNGCLALIEN GNLTIDELMT YITGPDFPTG AIINGRAGIV QAYRTGRGSV 250 260 270 280 290 300 YVRAKAEVEV DDKTSRETII IVHELPYQVN KARLIEKIAE LVKEKKVEGI SALRDESDKD 310 320 330 340 350 360 ACRIVIEIKR GESGEIVLNN LYKHTQLQTT FGINMVALDN NQPKVMNLKD ILDAFLLHRR 370 380 390 400 410 420 EVVTRRTVFE LRKARDRAHI LEGLAVALAN IDPIIELIRH SDTPADAKAK LVARGWELGN 430 440 450 460 470 480 VAAMLEKAGD DAARPEWLEP EFGIREGQYF LTEQQAQAIL DLRLHKLTGL EHGKILEEYQ 490 500 510 520 530 540 SLLDLIAELL FILASPERLM EVIRDELLAV REQYGDERPP EISASSAEIN IEDLITPEDV 550 560 570 580 590 600 VVTLSHQGYV KYQPITDYEA QRRGGRGKSA TRIKEENFVE RLLVANTHDT ILCFSTRGKV 610 620 630 640 650 660 YWLKVYQLPE ASRGARGRPI INLLPLEEGE RITAILPVKE YADDKYVFFA TADGTVKKTS 670 680 690 700 710 720 LSAFSRPLSS GIRAINLKEG DELIGVDITD GSNEIMLFSD AGKVVRFNEG SAAPMQPMLM 730 740 750 760 770 780 SSDDVDGDDE SVIDAGNDDD GSDNGEGSES TESKGTFKGV RPMGRTAGGV RGIRLLNGDK 790 800 810 820 830 840 VVSLIVPRGE GAILTATENG YGKGTALTEY PTKSRGTQGV RSIKVDEDGK VSIDQVDDTD 850 860 870 880 890 900 QIMLITNGGT LVRTRVSEVS IIGRNTGGVR LIRTGEDETV VGLQRIAESY EEENDVMAID 910 920 GEVSEGTDTA PDAGSAAADP EE

References

[1]	Oppegaard H. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2148/89.
[2]	"gyrA mutations in quinolone-resistant isolates of the fish pathogen Aeromonas salmonicida." Oppegaard H., Sorum H. Antimicrob. Agents Chemother. 38:2460-2464(1994) [PubMed: 7840589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-179. Strain: ATCC 14174 / NBRC 12659 / NCIMB 833.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L47978 Genomic DNA. Translation: AAB41037.1. L42453 Genomic DNA. Translation: AAA87239.1.
PIR	I39542.
3D structure databases
ProteinModelPortal	P48369.
SMR	P48369. Positions 30-523, 536-888.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_AERSA

Accession

Primary (citable) accession number: P48369

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program