P48368 (CRTE_CYAPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Geranylgeranyl pyrophosphate synthase homolog
Short name=GGPP synthase
EC=2.5.1.29

Alternative name(s):
Farnesyltranstransferase

Gene names

Name:

crtE

Encoded on

Plastid; Cyanelle

Organism

Cyanophora paradoxa

Taxonomic identifier

2762 [NCBI]

Taxonomic lineage

Eukaryota › Glaucocystophyceae › Cyanophoraceae › Cyanophora

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.
Subcellular location	Plastid › cyanelle.
Sequence similarities	Belongs to the FPP/GGPP synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Chlorophyll biosynthesis Isoprene biosynthesis Photosynthesis
Cellular component	Cyanelle Plastid
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cyanelle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	farnesyltranstransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Geranylgeranyl pyrophosphate synthase homolog		PRO_0000123996
Sites
	Metal binding	89	1	Magnesium 1 By similarity
	Metal binding	89	1	Magnesium 2 By similarity
	Metal binding	95	1	Magnesium 1 By similarity
	Metal binding	95	1	Magnesium 2 By similarity
	Metal binding	227	1	Magnesium 3 By similarity
	Binding site	50	1	Isopentenyl diphosphate By similarity
	Binding site	53	1	Isopentenyl diphosphate By similarity
	Binding site	82	1	Isopentenyl diphosphate By similarity
	Binding site	100	1	Farnesyl diphosphate By similarity
	Binding site	101	1	Isopentenyl diphosphate By similarity
	Binding site	186	1	Farnesyl diphosphate By similarity
	Binding site	187	1	Farnesyl diphosphate By similarity
	Binding site	224	1	Farnesyl diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48368 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 05950D0E3BA04B34	FASTA	300	33,008
	10 20 30 40 50 60 MKPLQTNFNL LTYLYERKEI VEDTLNKSIP RGNPTFIYDS IRYSLSAGGK RIRPILCLAS 70 80 90 100 110 120 CELAGGTMEM ALPTACALEM IHTMSLIHDD LPAMDNDSYR RGKPTNHIIY GEDLAILAGD 130 140 150 160 170 180 ALLAYAFEFI ATQTKNVPAD LIVKVIAQVA HSVTTSGLVG GQIIDLSSEG KSDTTLETLN 190 200 210 220 230 240 FIHIHKTGAL LEAAVLSGAL LAGAKEKDMN RFLRYAQNIG LAFQIIDDVL DIISTEEKLG 250 260 270 280 290 300 KSIGKDLKTQ KATYPSFWGV EESIKQAELL VEEAKEEILY FDNKAIPLIA IADFIVNRNN

References

[1]

"Nucleotide sequence of the cyanelle DNA from Cyanophora paradoxa." Stirewalt V.L., Michalowski C.B., Loeffelhardt W., Bohnert H.J., Bryant D.A. Plant Mol. Biol. Rep. 13:327-332(1995) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTEX LB 555 / Pringsheim.

[2]

"The complete sequence of the cyanelle genome of Cyanophora paradoxa: the genetic complexity of a primitive plastid." Loeffelhardt W., Stirewalt V.L., Michalowski C.B., Annarella M., Farley J.Y., Schluchter W.M., Chung S., Newmann-Spallart C., Steiner J.M., Jakowitsch J., Bohnert H.J., Bryant D.A. (In) Schenk H.E.A., Herrmann R., Jeon K.W., Mueller N.E., Schwemmler W. (eds.); Eukaryotism and symbiosis, pp.40-48, Springer-Verlag, Heidelberg (1997) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTEX LB 555 / Pringsheim.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30821 Genomic DNA. Translation: AAA81312.1.
PIR	T06969.
RefSeq	NP_043281.1. NC_001675.1.
3D structure databases
ProteinModelPortal	P48368.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	801601.
Phylogenomic databases
ProtClustDB	CHL00151.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS00723. POLYPRENYL_SYNTHASE_1. 1 hit. PS00444. POLYPRENYL_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTE_CYAPA

Accession

Primary (citable) accession number: P48368

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	September 21, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)