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Protein

Prefoldin subunit 3

Gene

PAC10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

GO - Molecular functioni

  • tubulin binding Source: SGD

GO - Biological processi

  • protein folding Source: InterPro
  • tubulin complex assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30790-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prefoldin subunit 3
Alternative name(s):
Genes involved in microtubule biogenesis protein 2
Gim complex subunit 2
Short name:
GimC subunit 2
Gene namesi
Name:PAC10
Synonyms:GIM2, PFD3
Ordered Locus Names:YGR078C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR078C.
SGDiS000003310. PAC10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • polysome Source: SGD
  • prefoldin complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Prefoldin subunit 3PRO_0000153659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP48363.
PeptideAtlasiP48363.

Interactioni

Subunit structurei

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GIM3P539004EBI-13239,EBI-13246
GIM5Q044936EBI-13239,EBI-13253
YKE2P525536EBI-13239,EBI-13260

GO - Molecular functioni

  • tubulin binding Source: SGD

Protein-protein interaction databases

BioGridi33320. 349 interactions.
DIPiDIP-6837N.
IntActiP48363. 11 interactions.
MINTiMINT-623878.

Structurei

3D structure databases

ProteinModelPortaliP48363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prefoldin subunit alpha family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018904.
HOGENOMiHOG000184744.
InParanoidiP48363.
OMAiDEQHSKY.
OrthoDBiEOG7DRJFG.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR016655. PFD3.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PANTHERiPTHR12409. PTHR12409. 1 hit.
PfamiPF02996. Prefoldin. 1 hit.
[Graphical view]
PIRSFiPIRSF016396. Prefoldin_subunit_3. 1 hit.
SUPFAMiSSF46579. SSF46579. 1 hit.

Sequencei

Sequence statusi: Complete.

P48363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTLFNSTEK NARGIPQAPF IENVNEIIKD PSDFELCFNK FQERLSKYKF
60 70 80 90 100
MQESKLATIK QLKTRIPDLE NTLKICQSLR NHSDEGDESD EPILLHYQLN
110 120 130 140 150
DTLYTKAQVD IPEDRADLKV GLWLGADVML EYPIDEAIEL LKKKLADSEQ
160 170 180 190
SLTVSTEDVE FLRENITTME VNCARLYNWD VQRRQDLKQA QEGTKNLKI
Length:199
Mass (Da):23,115
Last modified:February 1, 1996 - v1
Checksum:i884D7449C7AD6233
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29137 Genomic DNA. Translation: AAA70038.1.
Z72863 Genomic DNA. Translation: CAA97080.1.
AY557774 Genomic DNA. Translation: AAS56100.1.
BK006941 Genomic DNA. Translation: DAA08171.1.
PIRiS59741.
RefSeqiNP_011592.3. NM_001181207.3.

Genome annotation databases

EnsemblFungiiYGR078C; YGR078C; YGR078C.
GeneIDi852969.
KEGGisce:YGR078C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29137 Genomic DNA. Translation: AAA70038.1.
Z72863 Genomic DNA. Translation: CAA97080.1.
AY557774 Genomic DNA. Translation: AAS56100.1.
BK006941 Genomic DNA. Translation: DAA08171.1.
PIRiS59741.
RefSeqiNP_011592.3. NM_001181207.3.

3D structure databases

ProteinModelPortaliP48363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33320. 349 interactions.
DIPiDIP-6837N.
IntActiP48363. 11 interactions.
MINTiMINT-623878.

Proteomic databases

MaxQBiP48363.
PeptideAtlasiP48363.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR078C; YGR078C; YGR078C.
GeneIDi852969.
KEGGisce:YGR078C.

Organism-specific databases

EuPathDBiFungiDB:YGR078C.
SGDiS000003310. PAC10.

Phylogenomic databases

GeneTreeiENSGT00390000018904.
HOGENOMiHOG000184744.
InParanoidiP48363.
OMAiDEQHSKY.
OrthoDBiEOG7DRJFG.

Enzyme and pathway databases

BioCyciYEAST:G3O-30790-MONOMER.

Miscellaneous databases

NextBioi972753.
PROiP48363.

Family and domain databases

Gene3Di1.10.287.370. 1 hit.
InterProiIPR016655. PFD3.
IPR009053. Prefoldin.
IPR004127. Prefoldin_subunit_alpha.
[Graphical view]
PANTHERiPTHR12409. PTHR12409. 1 hit.
PfamiPF02996. Prefoldin. 1 hit.
[Graphical view]
PIRSFiPIRSF016396. Prefoldin_subunit_3. 1 hit.
SUPFAMiSSF46579. SSF46579. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Geiser J.R., Hoyt M.A.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "A novel protein complex promoting formation of functional alpha- and gamma-tubulin."
    Geissler S., Siegers K., Schiebel E.
    EMBO J. 17:952-966(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system."
    Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U.
    EMBO J. 18:75-84(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPFD3_YEAST
AccessioniPrimary (citable) accession number: P48363
Secondary accession number(s): D6VUL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.