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Protein

Probable NADPH:adrenodoxin oxidoreductase, mitochondrial

Gene

ARH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 reduced adrenodoxin + NADP+ = 2 oxidized adrenodoxin + NADPH.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261FAD; via amide nitrogenBy similarity
Binding sitei48 – 481FADBy similarity
Binding sitei56 – 561FAD; via amide nitrogenBy similarity
Binding sitei100 – 1001FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei235 – 2351NADPBy similarity
Binding sitei407 – 4071FAD; via amide nitrogenBy similarity
Binding sitei414 – 4141NADP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi177 – 1804NADPBy similarity
Nucleotide bindingi223 – 2242NADPBy similarity
Nucleotide bindingi414 – 4163FADBy similarity

GO - Molecular functioni

  • NADPH-adrenodoxin reductase activity Source: SGD

GO - Biological processi

  • cellular iron ion homeostasis Source: SGD
  • ubiquinone biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciMetaCyc:YDR376W-MONOMER.
YEAST:YDR376W-MONOMER.
ReactomeiR-SCE-2395516. Electron transport from NADPH to Ferredoxin.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADPH:adrenodoxin oxidoreductase, mitochondrial (EC:1.18.1.6)
Short name:
AR
Short name:
Adrenodoxin reductase
Alternative name(s):
Ferredoxin--NADP(+) reductase
Short name:
Ferredoxin reductase
Gene namesi
Name:ARH1
Ordered Locus Names:YDR376W
ORF Names:D9481.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR376W.
SGDiS000002784. ARH1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: SGD
  • mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 493Probable NADPH:adrenodoxin oxidoreductase, mitochondrialPRO_0000019425
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP48360.

Interactioni

Protein-protein interaction databases

BioGridi32435. 6 interactions.
DIPiDIP-1201N.
IntActiP48360. 2 interactions.
MINTiMINT-383441.

Structurei

3D structure databases

ProteinModelPortaliP48360.
SMRiP48360. Positions 16-59.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000013574.
HOGENOMiHOG000249250.
InParanoidiP48360.
KOiK18914.
OMAiMAPRCWR.
OrthoDBiEOG7HTHTK.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR021163. Ferredox_Rdtase_adrenod.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000362. FNR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48360-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFVQIRHIS SQINRKTVSI VGSGPSGFYT AYHLLKKSPI PLNVTIWEKL
60 70 80 90 100
PVPFGLSRYG VAPDHPEVKN CEETFTTCAE EFSSPTNQKH KFSFVGGITI
110 120 130 140 150
GKEILLKELL DNQDAVILSY GCTGDRKLNI PGELGTKGVF SSREFVNWYN
160 170 180 190 200
GHPDFAKDKR FTDFDWSKVS KVGIIGNGNV ALDITRVLIS NQIDEIWENT
210 220 230 240 250
DISSLALNLL RRAPVKDVKL IARRDFVHSK FTNKELRELW ELEKYGIRGR
260 270 280 290 300
IDPKFFQKEM FDPSKYDRAF NRRVEMCSEY LKPFNERSKK NYKKAPPPSS
310 320 330 340 350
GYDKFWELDY LKTPLKINRD DFGAINSLSL CNNRLNEDNS LQPLKDVNNI
360 370 380 390 400
MTYKVDLLIT SLGYAGVPMP EFSKLSIGFD KDHIANKQGR VLTSSGEIFP
410 420 430 440 450
HLYASGWIRK GSQGVIASTM QDAFEVGDRV IQDLVVSGAL SLENSIDLSN
460 470 480 490
IKHTTWKDWE RINKKELLRG KKEHKTRSKF LTFEELWNGV EGI
Length:493
Mass (Da):56,237
Last modified:February 1, 1996 - v1
Checksum:i9B0600BEA5A18CF3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331H → Y in AAC49500 (PubMed:8890749).Curated
Sequence conflicti132 – 1321G → S in AAA69523 (PubMed:9818721).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28038 Genomic DNA. Translation: AAA69523.1.
U38689 Genomic DNA. Translation: AAC49500.1.
U28373 Genomic DNA. Translation: AAB64812.1.
S47189 Genomic DNA. Translation: AAC09006.1.
BK006938 Genomic DNA. Translation: DAA12218.1.
PIRiS61171.
RefSeqiNP_010664.3. NM_001180684.3.

Genome annotation databases

EnsemblFungiiYDR376W; YDR376W; YDR376W.
GeneIDi851982.
KEGGisce:YDR376W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28038 Genomic DNA. Translation: AAA69523.1.
U38689 Genomic DNA. Translation: AAC49500.1.
U28373 Genomic DNA. Translation: AAB64812.1.
S47189 Genomic DNA. Translation: AAC09006.1.
BK006938 Genomic DNA. Translation: DAA12218.1.
PIRiS61171.
RefSeqiNP_010664.3. NM_001180684.3.

3D structure databases

ProteinModelPortaliP48360.
SMRiP48360. Positions 16-59.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32435. 6 interactions.
DIPiDIP-1201N.
IntActiP48360. 2 interactions.
MINTiMINT-383441.

Proteomic databases

MaxQBiP48360.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR376W; YDR376W; YDR376W.
GeneIDi851982.
KEGGisce:YDR376W.

Organism-specific databases

EuPathDBiFungiDB:YDR376W.
SGDiS000002784. ARH1.

Phylogenomic databases

GeneTreeiENSGT00390000013574.
HOGENOMiHOG000249250.
InParanoidiP48360.
KOiK18914.
OMAiMAPRCWR.
OrthoDBiEOG7HTHTK.

Enzyme and pathway databases

BioCyciMetaCyc:YDR376W-MONOMER.
YEAST:YDR376W-MONOMER.
ReactomeiR-SCE-2395516. Electron transport from NADPH to Ferredoxin.

Miscellaneous databases

PROiP48360.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR021163. Ferredox_Rdtase_adrenod.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000362. FNR. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ARH1 of Saccharomyces cerevisiae: a new essential gene that codes for a protein homologous to the human adrenodoxin reductase."
    Manzella L., Barros M.H., Nobrega F.G.
    Yeast 14:839-846(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A gene encoding a yeast equivalent of mammalian NADPH-adrenodoxin oxidoreductases."
    Lacour T., Dumas B.L.
    Gene 174:289-292(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: FL200.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "BCS1, a novel gene required for the expression of functional Rieske iron-sulfur protein in Saccharomyces cerevisiae."
    Nobrega F.G., Nobrega M.P., Tzagoloff A.
    EMBO J. 11:3821-3829(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiADRO_YEAST
AccessioniPrimary (citable) accession number: P48360
Secondary accession number(s): D6VT08, P32840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.