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P48360 (ADRO_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable NADPH:adrenodoxin oxidoreductase, mitochondrial
Short name=AR
Short name=Adrenodoxin reductase
EC=1.18.1.2
Alternative name(s):
Ferredoxin--NADP(+) reductase
Short name=Ferredoxin reductase
Gene names
Name:ARH1
Ordered Locus Names:YDR376W
ORF Names:D9481.5
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.

Cofactor

FAD By similarity.

Subcellular location

Mitochondrion matrix.

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the ferredoxin--NADP reductase type 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 493Probable NADPH:adrenodoxin oxidoreductase, mitochondrialPRO_0000019425

Regions

Nucleotide binding177 – 1804NADP By similarity
Nucleotide binding223 – 2242NADP By similarity
Nucleotide binding414 – 4163FAD By similarity

Sites

Binding site261FAD; via amide nitrogen By similarity
Binding site481FAD By similarity
Binding site561FAD; via amide nitrogen By similarity
Binding site1001FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding site2351NADP By similarity
Binding site4071FAD; via amide nitrogen By similarity
Binding site4141NADP; via amide nitrogen By similarity

Experimental info

Sequence conflict331H → Y in AAC49500. Ref.2
Sequence conflict1321G → S in AAA69523. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48360 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 9B0600BEA5A18CF3

FASTA49356,237
        10         20         30         40         50         60 
MSFVQIRHIS SQINRKTVSI VGSGPSGFYT AYHLLKKSPI PLNVTIWEKL PVPFGLSRYG 

        70         80         90        100        110        120 
VAPDHPEVKN CEETFTTCAE EFSSPTNQKH KFSFVGGITI GKEILLKELL DNQDAVILSY 

       130        140        150        160        170        180 
GCTGDRKLNI PGELGTKGVF SSREFVNWYN GHPDFAKDKR FTDFDWSKVS KVGIIGNGNV 

       190        200        210        220        230        240 
ALDITRVLIS NQIDEIWENT DISSLALNLL RRAPVKDVKL IARRDFVHSK FTNKELRELW 

       250        260        270        280        290        300 
ELEKYGIRGR IDPKFFQKEM FDPSKYDRAF NRRVEMCSEY LKPFNERSKK NYKKAPPPSS 

       310        320        330        340        350        360 
GYDKFWELDY LKTPLKINRD DFGAINSLSL CNNRLNEDNS LQPLKDVNNI MTYKVDLLIT 

       370        380        390        400        410        420 
SLGYAGVPMP EFSKLSIGFD KDHIANKQGR VLTSSGEIFP HLYASGWIRK GSQGVIASTM 

       430        440        450        460        470        480 
QDAFEVGDRV IQDLVVSGAL SLENSIDLSN IKHTTWKDWE RINKKELLRG KKEHKTRSKF 

       490 
LTFEELWNGV EGI

« Hide

References

« Hide 'large scale' references
[1]"ARH1 of Saccharomyces cerevisiae: a new essential gene that codes for a protein homologous to the human adrenodoxin reductase."
Manzella L., Barros M.H., Nobrega F.G.
Yeast 14:839-846(1998) [PubMed: 9818721] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A gene encoding a yeast equivalent of mammalian NADPH-adrenodoxin oxidoreductases."
Lacour T., Dumas B.L.
Gene 174:289-292(1996) [PubMed: 8890749] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: FL200.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"BCS1, a novel gene required for the expression of functional Rieske iron-sulfur protein in Saccharomyces cerevisiae."
Nobrega F.G., Nobrega M.P., Tzagoloff A.
EMBO J. 11:3821-3829(1992) [PubMed: 1327750] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U28038 Genomic DNA. Translation: AAA69523.1.
U38689 Genomic DNA. Translation: AAC49500.1.
U28373 Genomic DNA. Translation: AAB64812.1.
S47189 Genomic DNA. Translation: AAC09006.1.
BK006938 Genomic DNA. Translation: DAA12218.1.
PIRS61171.
RefSeqNP_010664.1. NM_001180684.1.

3D structure databases

ProteinModelPortalP48360.
SMRP48360. Positions 13-490.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1201N.
IntActP48360. 2 interactions.
MINTMINT-383441.
STRINGP48360.

Proteomic databases

PeptideAtlasP48360.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR376W; YDR376W; YDR376W.
GeneID851982.
KEGGsce:YDR376W.
NMPDRfig|4932.3.peg.1434.

Organism-specific databases

CYGDYDR376w.
SGDS000002784. ARH1.

Phylogenomic databases

eggNOGfuNOG06250.
GeneTreeEFGT00050000005726.
HOGENOMHBG522200.
OMADRIKEVP.
OrthoDBEOG4TQQJR.

Gene expression databases

ArrayExpressP48360.
GenevestigatorP48360.
GermOnlineYDR376W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR021163. Adrenodoxin_Rdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KOK00528.
PIRSFPIRSF000362. FNR. 1 hit.
ProtoNetSearch...

Other

NextBio970130.

Entry information

Entry nameADRO_YEAST
AccessionPrimary (citable) accession number: P48360
Secondary accession number(s): D6VT08, P32840
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents