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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner (PubMed:8878580) to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Enzyme regulationi

Inhibited by 4-quinoline drugs (nalidixic acid, ciprofloxacin, ofloxacin), although it is much less sensitive than the corresponding enzyme from E.coli (PubMed:8878580).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei130O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • DNA binding Source: UniProtKB-HAMAP
  • DNA supercoiling activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
Ordered Locus Names:MSMEG_0006, MSMEI_0008
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452421 – 842DNA gyrase subunit AAdd BLAST842

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains (PubMed:8878580). In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi246196.MSMEG_0006.

Structurei

3D structure databases

ProteinModelPortaliP48354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi538 – 544GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
HOGENOMiHOG000076278.
KOiK02469.
OMAiEMKSAYI.
OrthoDBiPOG091H025U.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.90.199.10. 1 hit.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiIPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

P48354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTTLPPEG EAHDRIEPVD IQQEMQRSYI DYAMSVIVGR ALPEVRDGLK
60 70 80 90 100
PVHRRVLYAM YDSGFRPDRS HAKSARSVAE TMGNYHPHGD ASIYDTLVRM
110 120 130 140 150
AQPWSLRYPL VDGQGNFGSP GNDPPAAMRY TEARLTPLAM EMLREIDEET
160 170 180 190 200
VDFIPNYDGR VQEPTVLPSR FPNLLANGSG GIAVGMATNI PPHNLGELAE
210 220 230 240 250
AVYWCLENYE ADEEATCEAV MERVKGPDFP TSGLIVGTQG IEDTYKTGRG
260 270 280 290 300
SIKMRGVVEI EEDSRGRTSI VITELPYQVN HDNFITSIAE QVRDGKLAGI
310 320 330 340 350
SNIEDQSSDR VGLRIVVELK RDAVAKVVLN NLYKHTQLQT SFGANMLSIV
360 370 380 390 400
DGVPRTLRLD QLIRLYVDHQ LDVIVRRTRY RLRKANERAH ILRGLVKALD
410 420 430 440 450
ALDEVIALIR ASQTVDIARA GLIELLDIDD IQAQAILDMQ LRRLAALERQ
460 470 480 490 500
KIVDDLAKIE AEIADLEDIL AKPERQRGIV RDELKEIVDK HGDARRTRIV
510 520 530 540 550
PADGEVSDED LIAREDVVVT ITETGYAKRT KTDLYRSQKR GGKGVQGAGL
560 570 580 590 600
KQDDMVNHFF VCSTHDWILF FTTQGRVYRA KAYELPEASR TARGQHVANL
610 620 630 640 650
LAFQPEERIA QVIQIKSYED APYLVLATRN GLVKKSKLSD FDSNRSGGIV
660 670 680 690 700
AINLREGDEL VGAVLCSAED DLLLVSANGQ SIRFSATDEA LRPMGRATSG
710 720 730 740 750
VQGMRFNEDD RLLSLNVVRP DTYLLVATSG GYAKRTSIDE YSVQGRGGKG
760 770 780 790 800
ILTIQYDRKR GSLVGALIVD DDTELYAITS TGGVIRTAAR QVRKAGRQTK
810 820 830 840
GVRLMNLAEG DTLIAIARNA DEDEAAESIS ESDADTAESP EA
Length:842
Mass (Da):93,183
Last modified:February 1, 1996 - v1
Checksum:i50AE5DBE80ED45DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94224 Genomic DNA. Translation: CAA63918.1.
CP000480 Genomic DNA. Translation: ABK69994.1.
CP001663 Genomic DNA. Translation: AFP36493.1.
RefSeqiWP_003891334.1. NZ_CP009494.1.
YP_884429.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK69994; ABK69994; MSMEG_0006.
AFP36493; AFP36493; MSMEI_0008.
GeneIDi4535988.
KEGGimsb:LJ00_00030.
msg:MSMEI_0008.
msm:MSMEG_0006.
PATRICi18072480. VBIMycSme59918_0006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94224 Genomic DNA. Translation: CAA63918.1.
CP000480 Genomic DNA. Translation: ABK69994.1.
CP001663 Genomic DNA. Translation: AFP36493.1.
RefSeqiWP_003891334.1. NZ_CP009494.1.
YP_884429.1. NC_008596.1.

3D structure databases

ProteinModelPortaliP48354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_0006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK69994; ABK69994; MSMEG_0006.
AFP36493; AFP36493; MSMEI_0008.
GeneIDi4535988.
KEGGimsb:LJ00_00030.
msg:MSMEI_0008.
msm:MSMEG_0006.
PATRICi18072480. VBIMycSme59918_0006.

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
HOGENOMiHOG000076278.
KOiK02469.
OMAiEMKSAYI.
OrthoDBiPOG091H025U.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.90.199.10. 1 hit.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiIPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGYRA_MYCS2
AccessioniPrimary (citable) accession number: P48354
Secondary accession number(s): A0QNE1, I7FC85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.