P48354 (GYRA_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	MSMEG_0006

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 842	842	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145242
Sites
	Active site	130	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48354 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 50AE5DBE80ED45DD	FASTA	842	93,183
	10 20 30 40 50 60 MTDTTLPPEG EAHDRIEPVD IQQEMQRSYI DYAMSVIVGR ALPEVRDGLK PVHRRVLYAM 70 80 90 100 110 120 YDSGFRPDRS HAKSARSVAE TMGNYHPHGD ASIYDTLVRM AQPWSLRYPL VDGQGNFGSP 130 140 150 160 170 180 GNDPPAAMRY TEARLTPLAM EMLREIDEET VDFIPNYDGR VQEPTVLPSR FPNLLANGSG 190 200 210 220 230 240 GIAVGMATNI PPHNLGELAE AVYWCLENYE ADEEATCEAV MERVKGPDFP TSGLIVGTQG 250 260 270 280 290 300 IEDTYKTGRG SIKMRGVVEI EEDSRGRTSI VITELPYQVN HDNFITSIAE QVRDGKLAGI 310 320 330 340 350 360 SNIEDQSSDR VGLRIVVELK RDAVAKVVLN NLYKHTQLQT SFGANMLSIV DGVPRTLRLD 370 380 390 400 410 420 QLIRLYVDHQ LDVIVRRTRY RLRKANERAH ILRGLVKALD ALDEVIALIR ASQTVDIARA 430 440 450 460 470 480 GLIELLDIDD IQAQAILDMQ LRRLAALERQ KIVDDLAKIE AEIADLEDIL AKPERQRGIV 490 500 510 520 530 540 RDELKEIVDK HGDARRTRIV PADGEVSDED LIAREDVVVT ITETGYAKRT KTDLYRSQKR 550 560 570 580 590 600 GGKGVQGAGL KQDDMVNHFF VCSTHDWILF FTTQGRVYRA KAYELPEASR TARGQHVANL 610 620 630 640 650 660 LAFQPEERIA QVIQIKSYED APYLVLATRN GLVKKSKLSD FDSNRSGGIV AINLREGDEL 670 680 690 700 710 720 VGAVLCSAED DLLLVSANGQ SIRFSATDEA LRPMGRATSG VQGMRFNEDD RLLSLNVVRP 730 740 750 760 770 780 DTYLLVATSG GYAKRTSIDE YSVQGRGGKG ILTIQYDRKR GSLVGALIVD DDTELYAITS 790 800 810 820 830 840 TGGVIRTAAR QVRKAGRQTK GVRLMNLAEG DTLIAIARNA DEDEAAESIS ESDADTAESP EA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis, purification, and study of inhibition by 4-quinolones of the DNA gyrase from Mycobacterium smegmatis." Revel-Viravau V., Truong Q.C., Moreau N., Jarlier V., Sougakoff W. Antimicrob. Agents Chemother. 40:2054-2061(1996) [PubMed: 8878580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94224 Genomic DNA. Translation: CAA63918.1. CP000480 Genomic DNA. Translation: ABK69994.1.
RefSeq	YP_884429.1. NC_008596.1.
3D structure databases
ProteinModelPortal	P48354.
ModBase	Search...
Protein-protein interaction databases
STRING	P48354.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000044470; EBMYCP00000042807; EBMYCG00000044465.
GeneID	4535988.
GenomeReviews	Gene locus MSMEG_0006 in contig CP000480_GR.
KEGG	msm:MSMEG_0006.
NMPDR	fig|246196.1.peg.6.
PATRIC	18072480. VBIMycSme59918_0006.
TIGR	MSMEG_0006.
Phylogenomic databases
eggNOG	COG0188.
GeneTree	EBGT00050000016653.
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_0006-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCS2

Accession

Primary (citable) accession number: P48354
Secondary accession number(s): A0QNE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program