Reviewed, UniProtKB/Swiss-Prot P48349 (14336_ARATH)

Names and origin

Protein names

Recommended name:
    14-3-3-like protein GF14 lambda
Alternative name(s):
    General regulatory factor 6
    14-3-3-like protein RCI2
    14-3-3-like protein AFT1

Gene names

Name:	GRF6
Synonyms:	AFT1, RCI2
Ordered Locus Names:	At5g10450
ORF Names:	F12B17_200

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes By similarity.
Induction	By cold.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Biological process	Stress response
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein domain specific binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BZR1	Q8S307	5	EBI-1633785,EBI-1803261
OMT1	Q9FK25	1	EBI-1633785,EBI-1633761

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P48349-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	14-3-3-like protein GF14 lambda		PRO_0000058668
Experimental info
	Sequence conflict	241 – 248	8	MQEQMDEA → YAGADGRGLRI in CAA52238. Ref.1
	Sequence conflict	243 – 248	6	EQMDEA → TNQMHHIRDIKEHVKTEITA KPCVLSYYYSM in CAB89398. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 4017D23098E74891	FASTA	248	27,976
	10 20 30 40 50 60 MAATLGRDQY VYMAKLAEQA ERYEEMVQFM EQLVTGATPA EELTVEERNL LSVAYKNVIG 70 80 90 100 110 120 SLRAAWRIVS SIEQKEESRK NDEHVSLVKD YRSKVESELS SVCSGILKLL DSHLIPSAGA 130 140 150 160 170 180 SESKVFYLKM KGDYHRYMAE FKSGDERKTA AEDTMLAYKA AQDIAAADMA PTHPIRLGLA 190 200 210 220 230 240 LNFSVFYYEI LNSSDKACNM AKQAFEEAIA ELDTLGEESY KDSTLIMQLL RDNLTLWTSD MQEQMDEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators." Jarillo J.A., Capel J., Leyva A., Martinez Zapater J.M., Salinas J. Plant Mol. Biol. 25:693-704(1994) [PubMed: 7520301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and expression of an Arabidopsis 14-3-3-like protein gene." Zhang H., Wang J., Hwang I., Goodman H.M. Biochim. Biophys. Acta 1266:113-116(1995) [PubMed: 7718616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[3]	"The Arabidopsis 14-3-3 multigene family." Wu K., Rooney M.F., Ferl R.J. Plant Physiol. 114:1421-1431(1997) [PubMed: 9276953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[4]	"Sequences of five Arabidopsis general regulatory factor (GRF) genes encoding 14-3-3 proteins." Chung H.-J., Shanker S., Ferl R.J. (er) Plant Gene Register PGR99-114 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X74141 mRNA. Translation: CAA52238.1. U02565 mRNA. Translation: AAA74737.1. U68545 mRNA. Translation: AAB08482.1. AF145298 Genomic DNA. Translation: AAD51781.1. AL353995 Genomic DNA. Translation: CAB89398.1. AY052293 mRNA. Translation: AAK96486.1. AY061918 mRNA. Translation: AAL31245.1.
PIR	S47970. S53727. T49994.
RefSeq	NP_568229.1.
UniGene	At.24269 At.71461
3D structure databases
HSSP	HSSP built from PDB template 1O9E based on UniProtKB P93343.
SMR	P48349. Positions 3-240.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48349.
Genome annotation databases
GeneID	830909.
GenomeReviews	Gene locus AT5G10450 in contig BA000015_GR.
NMPDR	fig|3702.1.peg.23175.
Organism-specific databases
TAIR	At5g10450.
Gene expression databases
ArrayExpress	P48349.
GermOnline	AT5G10450. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000308. 14-3-3. [Graphical view]
Gene3D	G3DSA:1.20.190.20. 14-3-3. 1 hit.
PANTHER	PTHR18860. 14-3-3. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
ProDom	PD000600. 14-3-3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

14336_ARATH

Accession

Primary (citable) accession number: P48349
Secondary accession number(s): P42647, Q9LX97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)