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Protein

14-3-3-like protein GF14 kappa

Gene

GRF8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes. Involved in the regulation of nutrient metabolism (PubMed:22104211). Negative regulator of freezing tolerance that modulates cold-responsive C-repeat-binding factors (CBF) DREB1A AND DREB1B proteins stability by facilitating their ubiquitin-mediated degradation (PubMed:28344081).2 Publications

GO - Molecular functioni

GO - Biological processi

  • regulation of metabolic process Source: UniProtKB
  • response to freezing Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-ATH-1445148. Translocation of GLUT4 to the plasma membrane.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3-like protein GF14 kappa
Alternative name(s):
General regulatory factor 8
Gene namesi
Name:GRF8
Ordered Locus Names:At5g65430
ORF Names:MNA5.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G65430.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Disturbed levels of several metabolites (e.g. beta-alanine, ribose, aspartate, pyroglutamate, glutamate, asparagine, fructose-6-phosphate, myo-inositol, 1,4-diaminobutane, palmitate and shikimate). Enhanced freezing tolerance associated with enhanced cold induction of cold-responsive C-repeat-binding factor (CBF) target genes in the double mutant lacking both GRF6 and GRF8, probably due to the suppression of ubiquitin-mediated degradation DREB1A and DREB1B degradation by the 26S proteasome pathway (PubMed:28344081).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586701 – 24814-3-3-like protein GF14 kappaAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineBy similarity1
Modified residuei112PhosphoserineBy similarity1
Modified residuei193PhosphoserineBy similarity1
Modified residuei214PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48348.
PRIDEiP48348.

PTM databases

iPTMnetiP48348.

Expressioni

Gene expression databases

ExpressionAtlasiP48348. baseline and differential.
GenevisibleiP48348. AT.

Interactioni

Subunit structurei

Interacts with the isocitrate dehydrogenase IDH3, and malate dehydrogenases MDH1 and MDH2.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi21910. 185 interactors.
IntActiP48348. 4 interactors.
MINTiMINT-7147278.
STRINGi3702.AT5G65430.3.

Structurei

3D structure databases

ProteinModelPortaliP48348.
SMRiP48348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
HOGENOMiHOG000240379.
PhylomeDBiP48348.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiView protein in InterPro
IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR036815. 14-3-3_dom_sf.
IPR023410. 14-3-3_domain.
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiView protein in Pfam
PF00244. 14-3-3. 1 hit.
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiView protein in SMART
SM00101. 14_3_3. 1 hit.
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiView protein in PROSITE
PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48348-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATTLSRDQY VYMAKLAEQA ERYEEMVQFM EQLVSGATPA GELTVEERNL
60 70 80 90 100
LSVAYKNVIG SLRAAWRIVS SIEQKEESRK NEEHVSLVKD YRSKVETELS
110 120 130 140 150
SICSGILRLL DSHLIPSATA SESKVFYLKM KGDYHRYLAE FKSGDERKTA
160 170 180 190 200
AEDTMIAYKA AQDVAVADLA PTHPIRLGLA LNFSVFYYEI LNSSEKACSM
210 220 230 240
AKQAFEEAIA ELDTLGEESY KDSTLIMQLL RDNLTLWTSD MQEQMDEA
Length:248
Mass (Da):28,029
Last modified:December 1, 2000 - v2
Checksum:i848897CE6DB6BA37
GO
Isoform 2 (identifier: P48348-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     243-244: Missing.

Note: No experimental confirmation available.
Show »
Length:246
Mass (Da):27,771
Checksum:i00B3F1CB3A37A53A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008971243 – 244Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36447 mRNA. Translation: AAA79700.2.
AF145300 Genomic DNA. Translation: AAD51783.1.
AB011479 Genomic DNA. Translation: BAB11565.1.
CP002688 Genomic DNA. Translation: AED98052.1.
CP002688 Genomic DNA. Translation: AED98053.1.
AY050996 mRNA. Translation: AAK93673.1.
AY079350 mRNA. Translation: AAL85081.1.
AK228019 mRNA. Translation: BAE99981.1.
AY085088 mRNA. Translation: AAM61642.1.
RefSeqiNP_569012.2. NM_125941.2. [P48348-2]
NP_851274.1. NM_180943.2. [P48348-1]
UniGeneiAt.10382.
At.24133.

Genome annotation databases

EnsemblPlantsiAT5G65430.1; AT5G65430.1; AT5G65430. [P48348-1]
AT5G65430.2; AT5G65430.2; AT5G65430. [P48348-2]
GeneIDi836668.
GrameneiAT5G65430.1; AT5G65430.1; AT5G65430.
AT5G65430.2; AT5G65430.2; AT5G65430.
KEGGiath:AT5G65430.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei14338_ARATH
AccessioniPrimary (citable) accession number: P48348
Secondary accession number(s): Q0WSB7, Q9SWP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 1, 2000
Last modified: October 25, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families