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Protein

14-3-3-like protein GF14 epsilon

Gene

GRF10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes.

GO - Molecular functioni

  • ATP binding Source: TAIR
  • protein domain specific binding Source: InterPro

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: TAIR
  • response to abscisic acid Source: TAIR

Enzyme and pathway databases

ReactomeiR-ATH-1445148. Translocation of GLUT4 to the plasma membrane.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3-like protein GF14 epsilon
Alternative name(s):
General regulatory factor 10
Gene namesi
Name:GRF10
Ordered Locus Names:At1g22300
ORF Names:T16E15.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G22300.
TAIRilocus:2196506. AT1G22300.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586721 – 25414-3-3-like protein GF14 epsilonAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineBy similarity1
Modified residuei188PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48347.
PRIDEiP48347.

PTM databases

iPTMnetiP48347.

Expressioni

Gene expression databases

ExpressionAtlasiP48347. baseline and differential.
GenevisibleiP48347. AT.

Interactioni

Subunit structurei

Interacts with DREB1A and DREB1B in the nucleus.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TOC34Q410092EBI-1803304,EBI-638506From Pisum sativum.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi24076. 80 interactors.
IntActiP48347. 4 interactors.
MINTiMINT-2584273.
STRINGi3702.AT1G22300.1.

Structurei

3D structure databases

ProteinModelPortaliP48347.
SMRiP48347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
HOGENOMiHOG000240379.
InParanoidiP48347.
KOiK06630.
OMAiESKGHEQ.
PhylomeDBiP48347.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiView protein in InterPro
IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiView protein in Pfam
PF00244. 14-3-3. 1 hit.
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiView protein in SMART
SM00101. 14_3_3. 1 hit.
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiView protein in PROSITE
PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48347-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENEREKQVY LAKLSEQTER YDEMVEAMKK VAQLDVELTV EERNLVSVGY
60 70 80 90 100
KNVIGARRAS WRILSSIEQK EESKGNDENV KRLKNYRKRV EDELAKVCND
110 120 130 140 150
ILSVIDKHLI PSSNAVESTV FFYKMKGDYY RYLAEFSSGA ERKEAADQSL
160 170 180 190 200
EAYKAAVAAA ENGLAPTHPV RLGLALNFSV FYYEILNSPE SACQLAKQAF
210 220 230 240 250
DDAIAELDSL NEESYKDSTL IMQLLRDNLT LWTSDLNEEG DERTKGADEP

QDEN
Length:254
Mass (Da):28,915
Last modified:February 1, 1996 - v1
Checksum:i037405C341845C25
GO
Isoform 2 (identifier: P48347-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-254: N → V

Show »
Length:254
Mass (Da):28,900
Checksum:i15F405C341845C25
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008972254N → V in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36446 mRNA. Translation: AAA79699.1.
AF145302 Genomic DNA. Translation: AAD51785.1.
AC068562 Genomic DNA. Translation: AAF87261.1.
CP002684 Genomic DNA. Translation: AEE30225.1.
CP002684 Genomic DNA. Translation: AEE30226.1.
AF334382 mRNA. Translation: AAG50088.1.
AY054505 mRNA. Translation: AAK96696.1.
AY058834 mRNA. Translation: AAL24222.1.
AY062838 mRNA. Translation: AAL32916.1.
AY081674 mRNA. Translation: AAM10236.1.
AY087580 mRNA. Translation: AAM65122.1.
PIRiH86355.
RefSeqiNP_564167.1. NM_102081.4. [P48347-1]
NP_849698.1. NM_179367.2. [P48347-2]
UniGeneiAt.24553.
At.67728.

Genome annotation databases

EnsemblPlantsiAT1G22300.1; AT1G22300.1; AT1G22300. [P48347-1]
AT1G22300.2; AT1G22300.2; AT1G22300. [P48347-2]
GeneIDi838837.
GrameneiAT1G22300.1; AT1G22300.1; AT1G22300.
AT1G22300.2; AT1G22300.2; AT1G22300.
KEGGiath:AT1G22300.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei14310_ARATH
AccessioniPrimary (citable) accession number: P48347
Secondary accession number(s): Q9LME5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: August 30, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families