Reviewed, UniProtKB/Swiss-Prot P48347 (14310_ARATH)

Names and origin

Protein names

Recommended name:
    14-3-3-like protein GF14 epsilon
Alternative name(s):
    General regulatory factor 10

Gene names

Name:	GRF10
Ordered Locus Names:	At1g22300
ORF Names:	T16E15.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein domain specific binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P48347-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P48347-2)

The sequence of this isoform differs from the canonical sequence as follows:
     254-254: N → V

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	14-3-3-like protein GF14 epsilon		PRO_0000058672
Natural variations
	Alternative sequence	254	1	N → V in isoform 2.		VSP_008972

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 037405C341845C25	FASTA	254	28,915
	10 20 30 40 50 60 MENEREKQVY LAKLSEQTER YDEMVEAMKK VAQLDVELTV EERNLVSVGY KNVIGARRAS 70 80 90 100 110 120 WRILSSIEQK EESKGNDENV KRLKNYRKRV EDELAKVCND ILSVIDKHLI PSSNAVESTV 130 140 150 160 170 180 FFYKMKGDYY RYLAEFSSGA ERKEAADQSL EAYKAAVAAA ENGLAPTHPV RLGLALNFSV 190 200 210 220 230 240 FYYEILNSPE SACQLAKQAF DDAIAELDSL NEESYKDSTL IMQLLRDNLT LWTSDLNEEG 250 DERTKGADEP QDEN
	Isoform 2 [UniParc]. Checksum: 15F405C341845C25		254	28,900
	10 20 30 40 50 60 MENEREKQVY LAKLSEQTER YDEMVEAMKK VAQLDVELTV EERNLVSVGY KNVIGARRAS 70 80 90 100 110 120 WRILSSIEQK EESKGNDENV KRLKNYRKRV EDELAKVCND ILSVIDKHLI PSSNAVESTV 130 140 150 160 170 180 FFYKMKGDYY RYLAEFSSGA ERKEAADQSL EAYKAAVAAA ENGLAPTHPV RLGLALNFSV 190 200 210 220 230 240 FYYEILNSPE SACQLAKQAF DDAIAELDSL NEESYKDSTL IMQLLRDNLT LWTSDLNEEG 250 DERTKGADEP QDEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis 14-3-3 multigene family." Wu K., Rooney M.F., Ferl R.J. Plant Physiol. 114:1421-1431(1997) [PubMed: 9276953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Columbia.
[2]	"Sequences of five Arabidopsis general regulatory factor (GRF) genes encoding 14-3-3 proteins." Chung H.-J., Shanker S., Ferl R.J. (er) Plant Gene Register PGR99-114 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Cross-references

Sequence databases
EMBLGenBankDDBJ	U36446 mRNA. Translation: AAA79699.1. AF145302 Genomic DNA. Translation: AAD51785.1. AC068562 Genomic DNA. Translation: AAF87261.1. AF334382 mRNA. Translation: AAG50088.1. AY054505 mRNA. Translation: AAK96696.1. AY058834 mRNA. Translation: AAL24222.1. AY062838 mRNA. Translation: AAL32916.1. AY081674 mRNA. Translation: AAM10236.1. AY087580 mRNA. Translation: AAM65122.1.
PIR	H86355.
RefSeq	NP_564167.1.
UniGene	At.24553
3D structure databases
HSSP	HSSP built from PDB template 1O9E based on UniProtKB P93343.
SMR	P48347. Positions 4-233.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48347.
Genome annotation databases
GeneID	838837.
GenomeReviews	Gene locus AT1G22300 in contig CT485782_GR.
KEGG	ath:AT1G22300.
NMPDR	fig|3702.1.peg.2596.
Organism-specific databases
TAIR	At1g22300.
Gene expression databases
ArrayExpress	P48347.
Family and domain databases
InterPro	IPR000308. 14-3-3. [Graphical view]
Gene3D	G3DSA:1.20.190.20. 14-3-3. 1 hit.
PANTHER	PTHR18860. 14-3-3. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
ProDom	PD000600. 14-3-3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

14310_ARATH

Accession

Primary (citable) accession number: P48347
Secondary accession number(s): Q9LME5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)