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Protein

Glutamate decarboxylase 2

Gene

GAD2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei558SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-SSC-888568. GABA synthesis.
R-SSC-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name:
GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene namesi
Name:GAD2
Synonyms:GAD65
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001469701 – 585Glutamate decarboxylase 2Add BLAST585

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei10PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Lipidationi30S-palmitoyl cysteineBy similarity1
Lipidationi45S-palmitoyl cysteineBy similarity1
Modified residuei396N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Phosphorylated; which does not affect kinetic parameters or subcellular location.By similarity
Palmitoylated; which is required for presynaptic clustering.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP48321.
PeptideAtlasiP48321.
PRIDEiP48321.

Expressioni

Gene expression databases

GenevisibleiP48321. SS.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011796.

Structurei

3D structure databases

ProteinModelPortaliP48321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 183Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiKOG0629. Eukaryota.
COG0076. LUCA.
GeneTreeiENSGT00760000119205.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiP48321.
KOiK01580.
OMAiWRAKGTT.
OrthoDBiEOG091G07ZU.
TreeFamiTF314688.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPGSGFWS FGSEDGSGDP ENSGTARAWC QVAQKFTGGI GNKLCALLYG
60 70 80 90 100
DAEKPAESGG SQPPRTTSRK ATCACNQKPC NCPKAEVNYA FLHATDLLPA
110 120 130 140 150
CDGERPTLAF LQDVMDILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
160 170 180 190 200
DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
210 220 230 240 250
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
260 270 280 290 300
MLIARFKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
310 320 330 340 350
LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV
360 370 380 390 400
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV
410 420 430 440 450
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
460 470 480 490 500
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYNIIKNREG YEMVFDGKPQ
510 520 530 540 550
HTNVCFWYVP PSLRVLDNNE ERMSRLSKVA PVIKARMMES GTTMVSYQPL
560 570 580
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
Length:585
Mass (Da):65,388
Last modified:February 1, 1996 - v1
Checksum:i4FF2810637671B6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31848 mRNA. Translation: BAA06635.1.
AY973276 mRNA. Translation: AAY28733.1.
PIRiJC4064.
RefSeqiNP_999060.2. NM_213895.2.
UniGeneiSsc.5021.

Genome annotation databases

EnsembliENSSSCT00000012104; ENSSSCP00000011796; ENSSSCG00000011060.
GeneIDi396929.
KEGGissc:396929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31848 mRNA. Translation: BAA06635.1.
AY973276 mRNA. Translation: AAY28733.1.
PIRiJC4064.
RefSeqiNP_999060.2. NM_213895.2.
UniGeneiSsc.5021.

3D structure databases

ProteinModelPortaliP48321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011796.

Proteomic databases

PaxDbiP48321.
PeptideAtlasiP48321.
PRIDEiP48321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000012104; ENSSSCP00000011796; ENSSSCG00000011060.
GeneIDi396929.
KEGGissc:396929.

Organism-specific databases

CTDi2572.

Phylogenomic databases

eggNOGiKOG0629. Eukaryota.
COG0076. LUCA.
GeneTreeiENSGT00760000119205.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiP48321.
KOiK01580.
OMAiWRAKGTT.
OrthoDBiEOG091G07ZU.
TreeFamiTF314688.

Enzyme and pathway databases

ReactomeiR-SSC-888568. GABA synthesis.
R-SSC-888590. GABA synthesis, release, reuptake and degradation.

Gene expression databases

GenevisibleiP48321. SS.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCE2_PIG
AccessioniPrimary (citable) accession number: P48321
Secondary accession number(s): Q506P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.