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P48321

- DCE2_PIG

UniProt

P48321 - DCE2_PIG

Protein

Glutamate decarboxylase 2

Gene

GAD2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the production of GABA.

    Catalytic activityi

    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Pyridoxal phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei558 – 5581SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate decarboxylase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. carboxylic acid metabolic process Source: InterPro
    2. neurotransmitter biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Neurotransmitter biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_207345. GABA synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase 2 (EC:4.1.1.15)
    Alternative name(s):
    65 kDa glutamic acid decarboxylase
    Short name:
    GAD-65
    Glutamate decarboxylase 65 kDa isoform
    Gene namesi
    Name:GAD2
    Synonyms:GAD65
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 10

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane By similarity; Lipid-anchor By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.By similarity

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. cell junction Source: UniProtKB-KW
    3. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    4. cytosol Source: UniProtKB-SubCell
    5. Golgi membrane Source: UniProtKB-SubCell
    6. presynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585Glutamate decarboxylase 2PRO_0000146970Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31PhosphoserineBy similarity
    Modified residuei6 – 61PhosphoserineBy similarity
    Modified residuei10 – 101PhosphoserineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity
    Lipidationi30 – 301S-palmitoyl cysteineBy similarity
    Lipidationi45 – 451S-palmitoyl cysteineBy similarity
    Modified residuei396 – 3961N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    Phosphorylated; which does not affect kinetic parameters or subcellular location.By similarity
    Palmitoylated; which is required for presynaptic clustering.By similarity

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000011796.

    Structurei

    3D structure databases

    ProteinModelPortaliP48321.
    SMRiP48321. Positions 88-584.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni181 – 1833Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    GeneTreeiENSGT00730000110441.
    HOGENOMiHOG000005382.
    HOVERGENiHBG004980.
    KOiK01580.
    OMAiWRAKGTT.
    OrthoDBiEOG7H1JM3.
    TreeFamiTF314688.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view]
    PfamiPF00282. Pyridoxal_deC. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48321-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPGSGFWS FGSEDGSGDP ENSGTARAWC QVAQKFTGGI GNKLCALLYG    50
    DAEKPAESGG SQPPRTTSRK ATCACNQKPC NCPKAEVNYA FLHATDLLPA 100
    CDGERPTLAF LQDVMDILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA 150
    DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA 200
    NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA 250
    MLIARFKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 300
    LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV 350
    ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV 400
    PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV 450
    DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYNIIKNREG YEMVFDGKPQ 500
    HTNVCFWYVP PSLRVLDNNE ERMSRLSKVA PVIKARMMES GTTMVSYQPL 550
    GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL 585
    Length:585
    Mass (Da):65,388
    Last modified:February 1, 1996 - v1
    Checksum:i4FF2810637671B6B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31848 mRNA. Translation: BAA06635.1.
    AY973276 mRNA. Translation: AAY28733.1.
    PIRiJC4064.
    RefSeqiNP_999060.2. NM_213895.2.
    UniGeneiSsc.5021.

    Genome annotation databases

    EnsembliENSSSCT00000012104; ENSSSCP00000011796; ENSSSCG00000011060.
    GeneIDi396929.
    KEGGissc:396929.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31848 mRNA. Translation: BAA06635.1 .
    AY973276 mRNA. Translation: AAY28733.1 .
    PIRi JC4064.
    RefSeqi NP_999060.2. NM_213895.2.
    UniGenei Ssc.5021.

    3D structure databases

    ProteinModelPortali P48321.
    SMRi P48321. Positions 88-584.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000011796.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000012104 ; ENSSSCP00000011796 ; ENSSSCG00000011060 .
    GeneIDi 396929.
    KEGGi ssc:396929.

    Organism-specific databases

    CTDi 2572.

    Phylogenomic databases

    eggNOGi COG0076.
    GeneTreei ENSGT00730000110441.
    HOGENOMi HOG000005382.
    HOVERGENi HBG004980.
    KOi K01580.
    OMAi WRAKGTT.
    OrthoDBi EOG7H1JM3.
    TreeFami TF314688.

    Enzyme and pathway databases

    Reactomei REACT_207345. GABA synthesis.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view ]
    Pfami PF00282. Pyridoxal_deC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences of two porcine glutamic acid decarboxylases (65- and 67-kDa GAD)."
      Suzuki R., Asami N., Amann E., Wagatsuma M.
      Gene 152:257-260(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. Yu H., Li J.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiDCE2_PIG
    AccessioniPrimary (citable) accession number: P48321
    Secondary accession number(s): Q506P9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3