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P48321

- DCE2_PIG

UniProt

P48321 - DCE2_PIG

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Protein
Glutamate decarboxylase 2
Gene
GAD2, GAD65
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Pyridoxal phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Substrate By similarity

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. carboxylic acid metabolic process Source: InterPro
  2. neurotransmitter biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_207345. GABA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name:
GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene namesi
Name:GAD2
Synonyms:GAD65
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 10

Subcellular locationi

Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane; Lipid-anchor By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity
Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. axon Source: Ensembl
  3. cell junction Source: UniProtKB-KW
  4. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  5. cytosol Source: UniProtKB-SubCell
  6. presynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Glutamate decarboxylase 2
PRO_0000146970Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31Phosphoserine By similarity
Modified residuei6 – 61Phosphoserine By similarity
Modified residuei10 – 101Phosphoserine By similarity
Modified residuei13 – 131Phosphoserine By similarity
Lipidationi30 – 301S-palmitoyl cysteine By similarity
Lipidationi45 – 451S-palmitoyl cysteine By similarity
Modified residuei396 – 3961N6-(pyridoxal phosphate)lysine By similarity

Post-translational modificationi

Phosphorylated; which does not affect kinetic parameters or subcellular location By similarity.
Palmitoylated; which is required for presynaptic clustering By similarity.

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011796.

Structurei

3D structure databases

ProteinModelPortaliP48321.
SMRiP48321. Positions 88-584.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1833Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0076.
GeneTreeiENSGT00730000110441.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
KOiK01580.
OMAiWRAKGTT.
OrthoDBiEOG7H1JM3.
TreeFamiTF314688.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48321-1 [UniParc]FASTAAdd to Basket

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MASPGSGFWS FGSEDGSGDP ENSGTARAWC QVAQKFTGGI GNKLCALLYG    50
DAEKPAESGG SQPPRTTSRK ATCACNQKPC NCPKAEVNYA FLHATDLLPA 100
CDGERPTLAF LQDVMDILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA 150
DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA 200
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA 250
MLIARFKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 300
LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV 350
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV 400
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV 450
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYNIIKNREG YEMVFDGKPQ 500
HTNVCFWYVP PSLRVLDNNE ERMSRLSKVA PVIKARMMES GTTMVSYQPL 550
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL 585
Length:585
Mass (Da):65,388
Last modified:February 1, 1996 - v1
Checksum:i4FF2810637671B6B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31848 mRNA. Translation: BAA06635.1.
AY973276 mRNA. Translation: AAY28733.1.
PIRiJC4064.
RefSeqiNP_999060.2. NM_213895.2.
UniGeneiSsc.5021.

Genome annotation databases

EnsembliENSSSCT00000012104; ENSSSCP00000011796; ENSSSCG00000011060.
GeneIDi396929.
KEGGissc:396929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31848 mRNA. Translation: BAA06635.1 .
AY973276 mRNA. Translation: AAY28733.1 .
PIRi JC4064.
RefSeqi NP_999060.2. NM_213895.2.
UniGenei Ssc.5021.

3D structure databases

ProteinModelPortali P48321.
SMRi P48321. Positions 88-584.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000011796.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000012104 ; ENSSSCP00000011796 ; ENSSSCG00000011060 .
GeneIDi 396929.
KEGGi ssc:396929.

Organism-specific databases

CTDi 2572.

Phylogenomic databases

eggNOGi COG0076.
GeneTreei ENSGT00730000110441.
HOGENOMi HOG000005382.
HOVERGENi HBG004980.
KOi K01580.
OMAi WRAKGTT.
OrthoDBi EOG7H1JM3.
TreeFami TF314688.

Enzyme and pathway databases

Reactomei REACT_207345. GABA synthesis.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view ]
Pfami PF00282. Pyridoxal_deC. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequences of two porcine glutamic acid decarboxylases (65- and 67-kDa GAD)."
    Suzuki R., Asami N., Amann E., Wagatsuma M.
    Gene 152:257-260(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Yu H., Li J.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDCE2_PIG
AccessioniPrimary (citable) accession number: P48321
Secondary accession number(s): Q506P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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