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Protein

Glutamate decarboxylase 2

Gene

Gad2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.1.1.15. 3474.
ReactomeiREACT_306908. GABA synthesis.
REACT_319523. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name:
GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene namesi
Name:Gad2
Synonyms:Gad65
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95634. Gad2.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: Ensembl
  • axon Source: MGI
  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • inhibitory synapse Source: MGI
  • perinuclear region of cytoplasm Source: Ensembl
  • presynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: MGI
  • synaptic vesicle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Glutamate decarboxylase 2PRO_0000146969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Lipidationi30 – 301S-palmitoyl cysteineBy similarity
Lipidationi45 – 451S-palmitoyl cysteineBy similarity
Modified residuei396 – 3961N6-(pyridoxal phosphate)lysineBy similarity

Post-translational modificationi

Phosphorylated; which does not affect kinetic parameters or subcellular location.By similarity
Palmitoylated; which is required for presynaptic clustering.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP48320.
PaxDbiP48320.
PRIDEiP48320.

PTM databases

PhosphoSiteiP48320.

Expressioni

Gene expression databases

BgeeiP48320.
CleanExiMM_GAD2.
ExpressionAtlasiP48320. baseline and differential.
GenevestigatoriP48320.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP48320. 2 interactions.
MINTiMINT-4996772.

Structurei

3D structure databases

ProteinModelPortaliP48320.
SMRiP48320. Positions 88-584.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1833Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiP48320.
KOiK01580.
OMAiWRAKGTT.
OrthoDBiEOG7H1JM3.
PhylomeDBiP48320.
TreeFamiTF314688.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPGSGFWS FGSEDGSADP ENPGTARAWC QVAQKFTGGI GNKLCALLYG
60 70 80 90 100
DSGKPAEGGG SVTSRAATGK VACTCDQKPC NCPKGDVNYA FLHATDLLPA
110 120 130 140 150
CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
160 170 180 190 200
DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
210 220 230 240 250
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
260 270 280 290 300
MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
310 320 330 340 350
LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV
360 370 380 390 400
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV
410 420 430 440 450
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
460 470 480 490 500
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYTIIKNREG YEMVFDGKPQ
510 520 530 540 550
HTNVCFWFVP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL
560 570 580
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
Length:585
Mass (Da):65,224
Last modified:February 1, 1996 - v1
Checksum:iC2F486E85123B057
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti259 – 2591F → S in BAA22893 (PubMed:8954991).Curated
Sequence conflicti319 – 3191I → S in S67454 (PubMed:8243324).Curated
Sequence conflicti325 – 3251K → E in BAA22893 (PubMed:8954991).Curated
Sequence conflicti499 – 4991P → S in BAA22893 (PubMed:8954991).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16980 mRNA. Translation: AAA93049.1.
D42051 mRNA. Translation: BAA22893.1.
BC018380 mRNA. Translation: AAH18380.1.
S67454 mRNA. No translation available.
CCDSiCCDS15724.1.
PIRiS38533.
RefSeqiNP_032104.2. NM_008078.2.
UniGeneiMm.4784.

Genome annotation databases

EnsembliENSMUST00000028123; ENSMUSP00000028123; ENSMUSG00000026787.
GeneIDi14417.
KEGGimmu:14417.
UCSCiuc008inj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16980 mRNA. Translation: AAA93049.1.
D42051 mRNA. Translation: BAA22893.1.
BC018380 mRNA. Translation: AAH18380.1.
S67454 mRNA. No translation available.
CCDSiCCDS15724.1.
PIRiS38533.
RefSeqiNP_032104.2. NM_008078.2.
UniGeneiMm.4784.

3D structure databases

ProteinModelPortaliP48320.
SMRiP48320. Positions 88-584.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48320. 2 interactions.
MINTiMINT-4996772.

PTM databases

PhosphoSiteiP48320.

Proteomic databases

MaxQBiP48320.
PaxDbiP48320.
PRIDEiP48320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028123; ENSMUSP00000028123; ENSMUSG00000026787.
GeneIDi14417.
KEGGimmu:14417.
UCSCiuc008inj.1. mouse.

Organism-specific databases

CTDi2572.
MGIiMGI:95634. Gad2.

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiP48320.
KOiK01580.
OMAiWRAKGTT.
OrthoDBiEOG7H1JM3.
PhylomeDBiP48320.
TreeFamiTF314688.

Enzyme and pathway databases

BRENDAi4.1.1.15. 3474.
ReactomeiREACT_306908. GABA synthesis.
REACT_319523. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

ChiTaRSiGad2. mouse.
NextBioi286001.
PROiP48320.
SOURCEiSearch...

Gene expression databases

BgeeiP48320.
CleanExiMM_GAD2.
ExpressionAtlasiP48320. baseline and differential.
GenevestigatoriP48320.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of a murine cDNA encoding glutamate decarboxylase (GAD65)."
    Lee D.S., Tian J., Phan T., Kaufman D.L.
    Biochim. Biophys. Acta 1216:157-160(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures."
    Asada H., Kawamura Y., Maruyama K., Kume H., Ding R.G., Ji F.Y., Kanbara N., Kuzume H., Sanbo M., Yagi T., Obata K.
    Biochem. Biophys. Res. Commun. 229:891-895(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. "Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat."
    Faulkner-Jones B.E., Cram D.S., Kun J., Harrison L.C.
    Endocrinology 133:2962-2972(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 175-379.
    Tissue: Brain.

Entry informationi

Entry nameiDCE2_MOUSE
AccessioniPrimary (citable) accession number: P48320
Secondary accession number(s): O35519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 1, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.