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P48320

- DCE2_MOUSE

UniProt

P48320 - DCE2_MOUSE

Protein

Glutamate decarboxylase 2

Gene

Gad2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the production of GABA.

    Catalytic activityi

    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Pyridoxal phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei558 – 5581SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate binding Source: Ensembl
    2. glutamate decarboxylase activity Source: UniProtKB-EC
    3. pyridoxal phosphate binding Source: Ensembl

    GO - Biological processi

    1. glutamate decarboxylation to succinate Source: Ensembl
    2. neurotransmitter biosynthetic process Source: UniProtKB-KW
    3. response to drug Source: Ensembl

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Neurotransmitter biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_198640. GABA synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase 2 (EC:4.1.1.15)
    Alternative name(s):
    65 kDa glutamic acid decarboxylase
    Short name:
    GAD-65
    Glutamate decarboxylase 65 kDa isoform
    Gene namesi
    Name:Gad2
    Synonyms:Gad65
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:95634. Gad2.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane By similarity; Lipid-anchor By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: Ensembl
    2. axon Source: MGI
    3. cell junction Source: UniProtKB-KW
    4. cytosol Source: UniProtKB-SubCell
    5. Golgi membrane Source: UniProtKB-SubCell
    6. perinuclear region of cytoplasm Source: Ensembl
    7. presynaptic membrane Source: UniProtKB-SubCell
    8. synapse Source: MGI
    9. synaptic vesicle membrane Source: Ensembl

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585Glutamate decarboxylase 2PRO_0000146969Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31PhosphoserineBy similarity
    Modified residuei6 – 61PhosphoserineBy similarity
    Modified residuei10 – 101PhosphoserineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity
    Lipidationi30 – 301S-palmitoyl cysteineBy similarity
    Lipidationi45 – 451S-palmitoyl cysteineBy similarity
    Modified residuei396 – 3961N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    Phosphorylated; which does not affect kinetic parameters or subcellular location.By similarity
    Palmitoylated; which is required for presynaptic clustering.By similarity

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP48320.
    PaxDbiP48320.
    PRIDEiP48320.

    PTM databases

    PhosphoSiteiP48320.

    Expressioni

    Gene expression databases

    BgeeiP48320.
    CleanExiMM_GAD2.
    GenevestigatoriP48320.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiP48320. 2 interactions.
    MINTiMINT-4996772.

    Structurei

    3D structure databases

    ProteinModelPortaliP48320.
    SMRiP48320. Positions 88-584.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni181 – 1833Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    HOGENOMiHOG000005382.
    HOVERGENiHBG004980.
    InParanoidiP48320.
    KOiK01580.
    OMAiWRAKGTT.
    OrthoDBiEOG7H1JM3.
    PhylomeDBiP48320.
    TreeFamiTF314688.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view]
    PfamiPF00282. Pyridoxal_deC. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48320-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPGSGFWS FGSEDGSADP ENPGTARAWC QVAQKFTGGI GNKLCALLYG    50
    DSGKPAEGGG SVTSRAATGK VACTCDQKPC NCPKGDVNYA FLHATDLLPA 100
    CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA 150
    DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA 200
    NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA 250
    MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 300
    LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV 350
    ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV 400
    PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV 450
    DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYTIIKNREG YEMVFDGKPQ 500
    HTNVCFWFVP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL 550
    GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL 585
    Length:585
    Mass (Da):65,224
    Last modified:February 1, 1996 - v1
    Checksum:iC2F486E85123B057
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti259 – 2591F → S in BAA22893. (PubMed:8954991)Curated
    Sequence conflicti319 – 3191I → S in S67454. (PubMed:8243324)Curated
    Sequence conflicti325 – 3251K → E in BAA22893. (PubMed:8954991)Curated
    Sequence conflicti499 – 4991P → S in BAA22893. (PubMed:8954991)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16980 mRNA. Translation: AAA93049.1.
    D42051 mRNA. Translation: BAA22893.1.
    BC018380 mRNA. Translation: AAH18380.1.
    S67454 mRNA. No translation available.
    CCDSiCCDS15724.1.
    PIRiS38533.
    RefSeqiNP_032104.2. NM_008078.2.
    UniGeneiMm.4784.

    Genome annotation databases

    EnsembliENSMUST00000028123; ENSMUSP00000028123; ENSMUSG00000026787.
    GeneIDi14417.
    KEGGimmu:14417.
    UCSCiuc008inj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16980 mRNA. Translation: AAA93049.1 .
    D42051 mRNA. Translation: BAA22893.1 .
    BC018380 mRNA. Translation: AAH18380.1 .
    S67454 mRNA. No translation available.
    CCDSi CCDS15724.1.
    PIRi S38533.
    RefSeqi NP_032104.2. NM_008078.2.
    UniGenei Mm.4784.

    3D structure databases

    ProteinModelPortali P48320.
    SMRi P48320. Positions 88-584.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48320. 2 interactions.
    MINTi MINT-4996772.

    PTM databases

    PhosphoSitei P48320.

    Proteomic databases

    MaxQBi P48320.
    PaxDbi P48320.
    PRIDEi P48320.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028123 ; ENSMUSP00000028123 ; ENSMUSG00000026787 .
    GeneIDi 14417.
    KEGGi mmu:14417.
    UCSCi uc008inj.1. mouse.

    Organism-specific databases

    CTDi 2572.
    MGIi MGI:95634. Gad2.

    Phylogenomic databases

    eggNOGi COG0076.
    HOGENOMi HOG000005382.
    HOVERGENi HBG004980.
    InParanoidi P48320.
    KOi K01580.
    OMAi WRAKGTT.
    OrthoDBi EOG7H1JM3.
    PhylomeDBi P48320.
    TreeFami TF314688.

    Enzyme and pathway databases

    Reactomei REACT_198640. GABA synthesis.

    Miscellaneous databases

    ChiTaRSi GAD2. mouse.
    NextBioi 286001.
    PROi P48320.
    SOURCEi Search...

    Gene expression databases

    Bgeei P48320.
    CleanExi MM_GAD2.
    Genevestigatori P48320.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view ]
    Pfami PF00282. Pyridoxal_deC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of a murine cDNA encoding glutamate decarboxylase (GAD65)."
      Lee D.S., Tian J., Phan T., Kaufman D.L.
      Biochim. Biophys. Acta 1216:157-160(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures."
      Asada H., Kawamura Y., Maruyama K., Kume H., Ding R.G., Ji F.Y., Kanbara N., Kuzume H., Sanbo M., Yagi T., Obata K.
      Biochem. Biophys. Res. Commun. 229:891-895(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    4. "Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat."
      Faulkner-Jones B.E., Cram D.S., Kun J., Harrison L.C.
      Endocrinology 133:2962-2972(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 175-379.
      Tissue: Brain.

    Entry informationi

    Entry nameiDCE2_MOUSE
    AccessioniPrimary (citable) accession number: P48320
    Secondary accession number(s): O35519
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3