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P48320 (DCE2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate decarboxylase 2

EC=4.1.1.15
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name=GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene names
Name:Gad2
Synonyms:Gad65
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the production of GABA.

Catalytic activity

L-glutamate = 4-aminobutanoate + CO2.

Cofactor

Pyridoxal phosphate.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane; Lipid-anchor By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.

Post-translational modification

Phosphorylated; which does not affect kinetic parameters or subcellular location By similarity.

Palmitoylated; which is required for presynaptic clustering By similarity.

Sequence similarities

Belongs to the group II decarboxylase family.

Ontologies

Keywords
   Biological processNeurotransmitter biosynthesis
   Cellular componentCell junction
Cell membrane
Cytoplasm
Cytoplasmic vesicle
Golgi apparatus
Membrane
Synapse
   LigandPyridoxal phosphate
   Molecular functionDecarboxylase
Lyase
   PTMLipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamate decarboxylation to succinate

Inferred from electronic annotation. Source: Ensembl

neurotransmitter biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

response to drug

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

anchored component of membrane

Inferred from electronic annotation. Source: Ensembl

axon

Inferred from direct assay PubMed 15479642. Source: MGI

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

presynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

synapse

Inferred from direct assay PubMed 15479642. Source: MGI

synaptic vesicle membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionglutamate binding

Inferred from electronic annotation. Source: Ensembl

glutamate decarboxylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585Glutamate decarboxylase 2
PRO_0000146969

Regions

Region181 – 1833Substrate binding By similarity

Sites

Binding site5581Substrate By similarity

Amino acid modifications

Modified residue31Phosphoserine By similarity
Modified residue61Phosphoserine By similarity
Modified residue101Phosphoserine By similarity
Modified residue131Phosphoserine By similarity
Modified residue3961N6-(pyridoxal phosphate)lysine By similarity
Lipidation301S-palmitoyl cysteine By similarity
Lipidation451S-palmitoyl cysteine By similarity

Experimental info

Sequence conflict2591F → S in BAA22893. Ref.2
Sequence conflict3191I → S in S67454. Ref.4
Sequence conflict3251K → E in BAA22893. Ref.2
Sequence conflict4991P → S in BAA22893. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P48320 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C2F486E85123B057

FASTA58565,224
        10         20         30         40         50         60 
MASPGSGFWS FGSEDGSADP ENPGTARAWC QVAQKFTGGI GNKLCALLYG DSGKPAEGGG 

        70         80         90        100        110        120 
SVTSRAATGK VACTCDQKPC NCPKGDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ 

       130        140        150        160        170        180 
YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL THCQTTLKYA IKTGHPRYFN 

       190        200        210        220        230        240 
QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS 

       250        260        270        280        290        300 
PGGAISNMYA MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 

       310        320        330        340        350        360 
LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW 

       370        380        390        400        410        420 
MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQSCNQ 

       430        440        450        460        470        480 
MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH IDKCLELAEY 

       490        500        510        520        530        540 
LYTIIKNREG YEMVFDGKPQ HTNVCFWFVP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY 

       550        560        570        580 
GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of a murine cDNA encoding glutamate decarboxylase (GAD65)."
Lee D.S., Tian J., Phan T., Kaufman D.L.
Biochim. Biophys. Acta 1216:157-160(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Brain.
[2]"Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures."
Asada H., Kawamura Y., Maruyama K., Kume H., Ding R.G., Ji F.Y., Kanbara N., Kuzume H., Sanbo M., Yagi T., Obata K.
Biochem. Biophys. Res. Commun. 229:891-895(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[4]"Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat."
Faulkner-Jones B.E., Cram D.S., Kun J., Harrison L.C.
Endocrinology 133:2962-2972(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 175-379.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L16980 mRNA. Translation: AAA93049.1.
D42051 mRNA. Translation: BAA22893.1.
BC018380 mRNA. Translation: AAH18380.1.
S67454 mRNA. No translation available.
PIRS38533.
RefSeqNP_032104.2. NM_008078.2.
UniGeneMm.4784.

3D structure databases

ProteinModelPortalP48320.
SMRP48320. Positions 88-584.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP48320. 1 interaction.
MINTMINT-4996772.

PTM databases

PhosphoSiteP48320.

Proteomic databases

PaxDbP48320.
PRIDEP48320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028123; ENSMUSP00000028123; ENSMUSG00000026787.
GeneID14417.
KEGGmmu:14417.
UCSCuc008inj.1. mouse.

Organism-specific databases

CTD2572.
MGIMGI:95634. Gad2.

Phylogenomic databases

eggNOGCOG0076.
HOGENOMHOG000005382.
HOVERGENHBG004980.
InParanoidP48320.
KOK01580.
OMAWRAKGTT.
OrthoDBEOG7H1JM3.
PhylomeDBP48320.
TreeFamTF314688.

Gene expression databases

BgeeP48320.
CleanExMM_GAD2.
GenevestigatorP48320.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGAD2. mouse.
NextBio286001.
PROP48320.
SOURCESearch...

Entry information

Entry nameDCE2_MOUSE
AccessionPrimary (citable) accession number: P48320
Secondary accession number(s): O35519
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot