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Protein

Glutamate decarboxylase 2

Gene

Gad2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei558SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processNeurotransmitter biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.1.1.15 3474
ReactomeiR-MMU-888568 GABA synthesis
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name:
GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene namesi
Name:Gad2
Synonyms:Gad65
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95634 Gad2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001469691 – 585Glutamate decarboxylase 2Add BLAST585

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei10PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Lipidationi30S-palmitoyl cysteineBy similarity1
Lipidationi45S-palmitoyl cysteineBy similarity1
Modified residuei396N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Phosphorylated; which does not affect kinetic parameters or subcellular location.By similarity
Palmitoylated; which is required for presynaptic clustering.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP48320
PeptideAtlasiP48320
PRIDEiP48320

PTM databases

iPTMnetiP48320
PhosphoSitePlusiP48320
SwissPalmiP48320

Expressioni

Gene expression databases

BgeeiENSMUSG00000026787
CleanExiMM_GAD2
ExpressionAtlasiP48320 baseline and differential
GenevisibleiP48320 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP48320, 2 interactors
MINTiP48320
STRINGi10090.ENSMUSP00000028123

Structurei

3D structure databases

ProteinModelPortaliP48320
SMRiP48320
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 183Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiKOG0629 Eukaryota
COG0076 LUCA
GeneTreeiENSGT00760000119205
HOGENOMiHOG000005382
HOVERGENiHBG004980
InParanoidiP48320
KOiK01580
OMAiWRAKGTT
OrthoDBiEOG091G07ZU
PhylomeDBiP48320
TreeFamiTF314688

Family and domain databases

Gene3Di3.40.640.10, 1 hit
InterProiView protein in InterPro
IPR002129 PyrdxlP-dep_de-COase
IPR015424 PyrdxlP-dep_Trfase
IPR015421 PyrdxlP-dep_Trfase_major
IPR021115 Pyridoxal-P_BS
PfamiView protein in Pfam
PF00282 Pyridoxal_deC, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00392 DDC_GAD_HDC_YDC, 1 hit

Sequencei

Sequence statusi: Complete.

P48320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPGSGFWS FGSEDGSADP ENPGTARAWC QVAQKFTGGI GNKLCALLYG
60 70 80 90 100
DSGKPAEGGG SVTSRAATGK VACTCDQKPC NCPKGDVNYA FLHATDLLPA
110 120 130 140 150
CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
160 170 180 190 200
DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
210 220 230 240 250
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
260 270 280 290 300
MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
310 320 330 340 350
LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV
360 370 380 390 400
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV
410 420 430 440 450
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
460 470 480 490 500
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYTIIKNREG YEMVFDGKPQ
510 520 530 540 550
HTNVCFWFVP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL
560 570 580
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
Length:585
Mass (Da):65,224
Last modified:February 1, 1996 - v1
Checksum:iC2F486E85123B057
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti259F → S in BAA22893 (PubMed:8954991).Curated1
Sequence conflicti319I → S in S67454 (PubMed:8243324).Curated1
Sequence conflicti325K → E in BAA22893 (PubMed:8954991).Curated1
Sequence conflicti499P → S in BAA22893 (PubMed:8954991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16980 mRNA Translation: AAA93049.1
D42051 mRNA Translation: BAA22893.1
BC018380 mRNA Translation: AAH18380.1
S67454 mRNA No translation available.
CCDSiCCDS15724.1
PIRiS38533
RefSeqiNP_032104.2, NM_008078.2
UniGeneiMm.4784

Genome annotation databases

EnsembliENSMUST00000028123; ENSMUSP00000028123; ENSMUSG00000026787
GeneIDi14417
KEGGimmu:14417
UCSCiuc008inj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDCE2_MOUSE
AccessioniPrimary (citable) accession number: P48320
Secondary accession number(s): O35519
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health