Glutamate decarboxylase 2 - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot P48320 (DCE2_MOUSE)

Last modified November 3, 2009. Version 83. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Glutamate decarboxylase 2
    EC=4.1.1.15
Alternative name(s):
    Glutamate decarboxylase 65 kDa isoform
    65 kDa glutamic acid decarboxylase
    GAD-65

Gene names

Name:	Gad2
Synonyms:	Gad65

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Catalyzes the production of GABA.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO₂.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm › cytosol By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse › presynaptic cell membrane; Lipid-anchor By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.
Post-translational modification	Phosphorylated; which does not affect kinetic parameters or subcellular location By similarity. Palmitoylated; which is required for presynaptic clustering By similarity.
Sequence similarities	Belongs to the group II decarboxylase family.

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Ontologies

Keywords
Biological process	Neurotransmitter biosynthesis
Cellular component	Cell junction Cell membrane Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane Synapse
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
PTM	Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell axon Inferred from direct assay. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 585

585

Glutamate decarboxylase 2

PRO_0000146969

Regions

Region

181 – 183

Substrate binding By similarity

Sites

Binding site

558

Substrate By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

396

N6-(pyridoxal phosphate)lysine By similarity

Lipidation

S-palmitoyl cysteine By similarity

Lipidation

S-palmitoyl cysteine By similarity

Experimental info

Sequence conflict

259

F → S in BAA22893. Ref.2

Sequence conflict

319

I → S in S67454. Ref.4

Sequence conflict

325

K → E in BAA22893. Ref.2

Sequence conflict

499

P → S in BAA22893. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P48320-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C2F486E85123B057
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FASTA

585

65,224

        10         20         30         40         50         60 
MASPGSGFWS FGSEDGSADP ENPGTARAWC QVAQKFTGGI GNKLCALLYG DSGKPAEGGG 

        70         80         90        100        110        120 
SVTSRAATGK VACTCDQKPC NCPKGDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ 

       130        140        150        160        170        180 
YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL THCQTTLKYA IKTGHPRYFN 

       190        200        210        220        230        240 
QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS 

       250        260        270        280        290        300 
PGGAISNMYA MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 

       310        320        330        340        350        360 
LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW 

       370        380        390        400        410        420 
MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQSCNQ 

       430        440        450        460        470        480 
MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH IDKCLELAEY 

       490        500        510        520        530        540 
LYTIIKNREG YEMVFDGKPQ HTNVCFWFVP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY 

       550        560        570        580 
GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of a murine cDNA encoding glutamate decarboxylase (GAD65)." Lee D.S., Tian J., Phan T., Kaufman D.L. Biochim. Biophys. Acta 1216:157-160(1993) [PubMed: 8218409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures." Asada H., Kawamura Y., Maruyama K., Kume H., Ding R.G., Ji F.Y., Kanbara N., Kuzume H., Sanbo M., Yagi T., Obata K. Biochem. Biophys. Res. Commun. 229:891-895(1996) [PubMed: 8954991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[4]	"Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat." Faulkner-Jones B.E., Cram D.S., Kun J., Harrison L.C. Endocrinology 133:2962-2972(1993) [PubMed: 8243324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 175-379. Tissue: Brain.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	L16980 mRNA. Translation: AAA93049.1. D42051 mRNA. Translation: BAA22893.1. BC018380 mRNA. Translation: AAH18380.1. S67454 mRNA. No translation available.
IPI	IPI00318522.
PIR	S38533.
RefSeq	NP_032104.2.
UniGene	Mm.4784
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P48320.
Proteomic databases
PRIDE	P48320.
Genome annotation databases
Ensembl	ENSMUST00000028123; ENSMUSP00000028123; ENSMUSG00000026787; Mus musculus. [Genome view]
GeneID	14417.
KEGG	mmu:14417.
UCSC	uc008ink.1. mouse.
Organism-specific databases
CTD	14417.
MGI	MGI:95634. Gad2.
Phylogenomic databases
HOGENOM	P48320.
HOVERGEN	P48320.
OMA	WRAKGTT.
Enzyme and pathway databases
BRENDA	4.1.1.15. 244.
Gene expression databases
ArrayExpress	P48320.
Bgee	P48320.
CleanEx	MM_GAD2.
Genevestigator	P48320.
GermOnline	ENSMUSG00000026787. Mus musculus.
Family and domain databases
InterPro	IPR002129. PyrdxlP-dep_de-COase. [Graphical view]
PANTHER	PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
PROSITE	PS00392. DDC_GAD_HDC_YDC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	286001.
SOURCE	Search...

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Entry information

Entry name

DCE2_MOUSE

Accession

Primary (citable) accession number: P48320
Secondary accession number(s): O35519

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents