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P48318

- DCE1_MOUSE

UniProt

P48318 - DCE1_MOUSE

Protein

Glutamate decarboxylase 1

Gene

Gad1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (13 Dec 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the production of GABA.

    Catalytic activityi

    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Pyridoxal phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei566 – 5661SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate binding Source: Ensembl
    2. glutamate decarboxylase activity Source: UniProtKB-EC
    3. pyridoxal phosphate binding Source: Ensembl

    GO - Biological processi

    1. gamma-aminobutyric acid biosynthetic process Source: Ensembl
    2. neurotransmitter biosynthetic process Source: UniProtKB-KW
    3. response to drug Source: Ensembl

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Neurotransmitter biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_198640. GABA synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase 1 (EC:4.1.1.15)
    Alternative name(s):
    67 kDa glutamic acid decarboxylase
    Short name:
    GAD-67
    Glutamate decarboxylase 67 kDa isoform
    Gene namesi
    Name:Gad1
    Synonyms:Gad67
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:95632. Gad1.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: MGI
    2. axon terminus Source: MGI
    3. cell cortex Source: MGI
    4. cytoplasm Source: MGI
    5. mitochondrion Source: MGI
    6. neuron projection terminus Source: MGI
    7. presynaptic active zone Source: MGI
    8. synapse Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 593593Glutamate decarboxylase 1PRO_0000146964Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei404 – 4041N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiP48318.
    PaxDbiP48318.
    PRIDEiP48318.

    PTM databases

    PhosphoSiteiP48318.

    Expressioni

    Gene expression databases

    ArrayExpressiP48318.
    BgeeiP48318.
    CleanExiMM_GAD1.
    GenevestigatoriP48318.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiP48318. 2 interactions.
    MINTiMINT-4092862.

    Structurei

    3D structure databases

    ProteinModelPortaliP48318.
    SMRiP48318. Positions 92-592.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni189 – 1913Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    HOGENOMiHOG000005382.
    HOVERGENiHBG004980.
    InParanoidiP48318.
    KOiK01580.
    OMAiTNLRPPG.
    OrthoDBiEOG7H1JM3.
    PhylomeDBiP48318.
    TreeFamiTF314688.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view]
    PfamiPF00282. Pyridoxal_deC. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48318-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL    50
    QRTNSLEEKS RLVSAFRERQ SSKNLLSCEN SDQGARFRRT ETDFSNLFAQ 100
    DLLPAKNGEE QTAQFLLEVV DILLNYVRKT FDRSTKVLDF HHPHQLLEGM 150
    EGFNLELSDH PESLEQILVD CRDTLKYGVR TGHPRFFNQL STGLDIIGLA 200
    GEWLTSTANT NMFTYEIAPV FVLMEQITLK KMREIVGWSN KDGDGIFSPG 250
    GAISNMYSIM AARYKYFPEV KTKGMAAVPK LVLFTSEHSH YSIKKAGAAL 300
    GFGTDNVILI KCNERGKIIP ADLEAKILDA KQKGYVPLYV NATAGTTVYG 350
    AFDPIQEIAD ICEKYNLWLH VDAAWGGGLL MSRKHRHKLS GIERANSVTW 400
    NPHKMMGVLL QCSAILVKEK GILQGCNQMC AGYLFQPDKQ YDVSYDTGDK 450
    AIQCGRHVDI FKFWLMWKAK GTVGFENQIN KCLELADYLY AKIKNREEFE 500
    MVFDGEPEHT NVCFWYIPQS LRGVPDSPER REKLHRVAPK IKALMMESGT 550
    TMVGYQPQGD KANFFRMVIS NPAATQSDID FLIEEIERLG QDL 593
    Length:593
    Mass (Da):66,648
    Last modified:December 13, 2002 - v2
    Checksum:i82D1AAF216F25100
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti133 – 1331R → H in CAA90277. (PubMed:12106047)Curated
    Sequence conflicti234 – 2341E → K in S67453. (PubMed:8243324)Curated
    Sequence conflicti258 – 2581S → T in CAA90277. (PubMed:12106047)Curated
    Sequence conflicti360 – 3601D → S in CAA90277. (PubMed:12106047)Curated
    Sequence conflicti461 – 4611F → N in CAA90277. (PubMed:12106047)Curated
    Sequence conflicti554 – 5541G → A in CAA90277. (PubMed:12106047)Curated
    Sequence conflicti575 – 5751T → S in CAA90277. (PubMed:12106047)Curated
    Sequence conflicti583 – 5831I → T in CAA90277. (PubMed:12106047)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49976 mRNA. Translation: CAA90277.1.
    Y12257 mRNA. Translation: CAA72934.1.
    AF483492 mRNA. Translation: AAL90766.1.
    AF483493 mRNA. Translation: AAL90767.1.
    BC027059 mRNA. Translation: AAH27059.1.
    S67453 mRNA. No translation available.
    CCDSiCCDS16108.1.
    PIRiS61534.
    RefSeqiNP_032103.2. NM_008077.4.
    XP_006498828.1. XM_006498765.1.
    XP_006498829.1. XM_006498766.1.
    UniGeneiMm.272120.

    Genome annotation databases

    EnsembliENSMUST00000094934; ENSMUSP00000092539; ENSMUSG00000070880.
    GeneIDi14415.
    KEGGimmu:14415.
    UCSCiuc008jzk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49976 mRNA. Translation: CAA90277.1 .
    Y12257 mRNA. Translation: CAA72934.1 .
    AF483492 mRNA. Translation: AAL90766.1 .
    AF483493 mRNA. Translation: AAL90767.1 .
    BC027059 mRNA. Translation: AAH27059.1 .
    S67453 mRNA. No translation available.
    CCDSi CCDS16108.1.
    PIRi S61534.
    RefSeqi NP_032103.2. NM_008077.4.
    XP_006498828.1. XM_006498765.1.
    XP_006498829.1. XM_006498766.1.
    UniGenei Mm.272120.

    3D structure databases

    ProteinModelPortali P48318.
    SMRi P48318. Positions 92-592.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48318. 2 interactions.
    MINTi MINT-4092862.

    PTM databases

    PhosphoSitei P48318.

    Proteomic databases

    MaxQBi P48318.
    PaxDbi P48318.
    PRIDEi P48318.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000094934 ; ENSMUSP00000092539 ; ENSMUSG00000070880 .
    GeneIDi 14415.
    KEGGi mmu:14415.
    UCSCi uc008jzk.1. mouse.

    Organism-specific databases

    CTDi 2571.
    MGIi MGI:95632. Gad1.

    Phylogenomic databases

    eggNOGi COG0076.
    HOGENOMi HOG000005382.
    HOVERGENi HBG004980.
    InParanoidi P48318.
    KOi K01580.
    OMAi TNLRPPG.
    OrthoDBi EOG7H1JM3.
    PhylomeDBi P48318.
    TreeFami TF314688.

    Enzyme and pathway databases

    Reactomei REACT_198640. GABA synthesis.

    Miscellaneous databases

    NextBioi 285997.
    PROi P48318.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48318.
    Bgeei P48318.
    CleanExi MM_GAD1.
    Genevestigatori P48318.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view ]
    Pfami PF00282. Pyridoxal_deC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular identification of the 62 kd form of glutamic acid decarboxylase from the mouse."
      Katarova Z., Szabo G., Mugnaini E., Greenspan R.
      Eur. J. Neurosci. 2:190-202(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. Aust G., Steinbrenner H., Thamm B., Rost A.K., Seissler J.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    3. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
      Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
      Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ILS and ISS.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    5. "Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat."
      Faulkner-Jones B.E., Cram D.S., Kun J., Harrison L.C.
      Endocrinology 133:2962-2972(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 198-403.
      Tissue: Brain.

    Entry informationi

    Entry nameiDCE1_MOUSE
    AccessioniPrimary (citable) accession number: P48318
    Secondary accession number(s): O08685
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: December 13, 2002
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3