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P48318

- DCE1_MOUSE

UniProt

P48318 - DCE1_MOUSE

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Protein

Glutamate decarboxylase 1

Gene

Gad1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661SubstrateBy similarity

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. carboxylic acid metabolic process Source: InterPro
  2. neurotransmitter biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_198640. GABA synthesis.
REACT_245631. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 1 (EC:4.1.1.15)
Alternative name(s):
67 kDa glutamic acid decarboxylase
Short name:
GAD-67
Glutamate decarboxylase 67 kDa isoform
Gene namesi
Name:Gad1
Synonyms:Gad67
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:95632. Gad1.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: MGI
  2. axon terminus Source: MGI
  3. cell cortex Source: MGI
  4. cytoplasm Source: MGI
  5. mitochondrion Source: MGI
  6. neuron projection terminus Source: MGI
  7. presynaptic active zone Source: MGI
  8. synapse Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Glutamate decarboxylase 1PRO_0000146964Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei404 – 4041N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiP48318.
PaxDbiP48318.
PRIDEiP48318.

PTM databases

PhosphoSiteiP48318.

Expressioni

Gene expression databases

BgeeiP48318.
CleanExiMM_GAD1.
ExpressionAtlasiP48318. baseline and differential.
GenevestigatoriP48318.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP48318. 2 interactions.
MINTiMINT-4092862.

Structurei

3D structure databases

ProteinModelPortaliP48318.
SMRiP48318. Positions 92-592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1913Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiP48318.
KOiK01580.
OMAiTNLRPPG.
OrthoDBiEOG7H1JM3.
PhylomeDBiP48318.
TreeFamiTF314688.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48318-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL
60 70 80 90 100
QRTNSLEEKS RLVSAFRERQ SSKNLLSCEN SDQGARFRRT ETDFSNLFAQ
110 120 130 140 150
DLLPAKNGEE QTAQFLLEVV DILLNYVRKT FDRSTKVLDF HHPHQLLEGM
160 170 180 190 200
EGFNLELSDH PESLEQILVD CRDTLKYGVR TGHPRFFNQL STGLDIIGLA
210 220 230 240 250
GEWLTSTANT NMFTYEIAPV FVLMEQITLK KMREIVGWSN KDGDGIFSPG
260 270 280 290 300
GAISNMYSIM AARYKYFPEV KTKGMAAVPK LVLFTSEHSH YSIKKAGAAL
310 320 330 340 350
GFGTDNVILI KCNERGKIIP ADLEAKILDA KQKGYVPLYV NATAGTTVYG
360 370 380 390 400
AFDPIQEIAD ICEKYNLWLH VDAAWGGGLL MSRKHRHKLS GIERANSVTW
410 420 430 440 450
NPHKMMGVLL QCSAILVKEK GILQGCNQMC AGYLFQPDKQ YDVSYDTGDK
460 470 480 490 500
AIQCGRHVDI FKFWLMWKAK GTVGFENQIN KCLELADYLY AKIKNREEFE
510 520 530 540 550
MVFDGEPEHT NVCFWYIPQS LRGVPDSPER REKLHRVAPK IKALMMESGT
560 570 580 590
TMVGYQPQGD KANFFRMVIS NPAATQSDID FLIEEIERLG QDL
Length:593
Mass (Da):66,648
Last modified:December 13, 2002 - v2
Checksum:i82D1AAF216F25100
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331R → H in CAA90277. (PubMed:12106047)Curated
Sequence conflicti234 – 2341E → K in S67453. (PubMed:8243324)Curated
Sequence conflicti258 – 2581S → T in CAA90277. (PubMed:12106047)Curated
Sequence conflicti360 – 3601D → S in CAA90277. (PubMed:12106047)Curated
Sequence conflicti461 – 4611F → N in CAA90277. (PubMed:12106047)Curated
Sequence conflicti554 – 5541G → A in CAA90277. (PubMed:12106047)Curated
Sequence conflicti575 – 5751T → S in CAA90277. (PubMed:12106047)Curated
Sequence conflicti583 – 5831I → T in CAA90277. (PubMed:12106047)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49976 mRNA. Translation: CAA90277.1.
Y12257 mRNA. Translation: CAA72934.1.
AF483492 mRNA. Translation: AAL90766.1.
AF483493 mRNA. Translation: AAL90767.1.
BC027059 mRNA. Translation: AAH27059.1.
S67453 mRNA. No translation available.
CCDSiCCDS16108.1.
PIRiS61534.
RefSeqiNP_032103.2. NM_008077.4.
XP_006498828.1. XM_006498765.1.
XP_006498829.1. XM_006498766.1.
UniGeneiMm.272120.

Genome annotation databases

EnsembliENSMUST00000094934; ENSMUSP00000092539; ENSMUSG00000070880.
GeneIDi14415.
KEGGimmu:14415.
UCSCiuc008jzk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49976 mRNA. Translation: CAA90277.1 .
Y12257 mRNA. Translation: CAA72934.1 .
AF483492 mRNA. Translation: AAL90766.1 .
AF483493 mRNA. Translation: AAL90767.1 .
BC027059 mRNA. Translation: AAH27059.1 .
S67453 mRNA. No translation available.
CCDSi CCDS16108.1.
PIRi S61534.
RefSeqi NP_032103.2. NM_008077.4.
XP_006498828.1. XM_006498765.1.
XP_006498829.1. XM_006498766.1.
UniGenei Mm.272120.

3D structure databases

ProteinModelPortali P48318.
SMRi P48318. Positions 92-592.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P48318. 2 interactions.
MINTi MINT-4092862.

PTM databases

PhosphoSitei P48318.

Proteomic databases

MaxQBi P48318.
PaxDbi P48318.
PRIDEi P48318.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000094934 ; ENSMUSP00000092539 ; ENSMUSG00000070880 .
GeneIDi 14415.
KEGGi mmu:14415.
UCSCi uc008jzk.1. mouse.

Organism-specific databases

CTDi 2571.
MGIi MGI:95632. Gad1.

Phylogenomic databases

eggNOGi COG0076.
HOGENOMi HOG000005382.
HOVERGENi HBG004980.
InParanoidi P48318.
KOi K01580.
OMAi TNLRPPG.
OrthoDBi EOG7H1JM3.
PhylomeDBi P48318.
TreeFami TF314688.

Enzyme and pathway databases

Reactomei REACT_198640. GABA synthesis.
REACT_245631. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

NextBioi 285997.
PROi P48318.
SOURCEi Search...

Gene expression databases

Bgeei P48318.
CleanExi MM_GAD1.
ExpressionAtlasi P48318. baseline and differential.
Genevestigatori P48318.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view ]
Pfami PF00282. Pyridoxal_deC. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular identification of the 62 kd form of glutamic acid decarboxylase from the mouse."
    Katarova Z., Szabo G., Mugnaini E., Greenspan R.
    Eur. J. Neurosci. 2:190-202(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Aust G., Steinbrenner H., Thamm B., Rost A.K., Seissler J.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
    Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
    Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ILS and ISS.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  5. "Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat."
    Faulkner-Jones B.E., Cram D.S., Kun J., Harrison L.C.
    Endocrinology 133:2962-2972(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 198-403.
    Tissue: Brain.

Entry informationi

Entry nameiDCE1_MOUSE
AccessioniPrimary (citable) accession number: P48318
Secondary accession number(s): O08685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 13, 2002
Last modified: November 26, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3