Reviewed, UniProtKB/Swiss-Prot P48318 (DCE1_MOUSE)

Names and origin

Protein names

Recommended name:
    Glutamate decarboxylase 1
    EC=4.1.1.15
Alternative name(s):
    Glutamate decarboxylase 67 kDa isoform
    67 kDa glutamic acid decarboxylase
    GAD-67

Gene names

Name:	Gad1
Synonyms:	Gad67

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the production of GABA.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the group II decarboxylase family.

Ontologies

Keywords
Biological process	Neurotransmitter biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Gene Ontology (GO)
Biological process	carboxylic acid metabolic process Inferred from electronic annotation. Source: InterPro neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	axon Inferred from direct assay. Source: MGI mitochondrion Inferred from direct assay. Source: MGI synapse Inferred from direct assay. Source: MGI
Molecular function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Glutamate decarboxylase 1		PRO_0000146964
Regions
	Region	189 – 191	3	Substrate binding By similarity
Sites
	Binding site	566	1	Substrate By similarity
Amino acid modifications
	Modified residue	404	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	133	1	R → H in CAA90277. Ref.1
	Sequence conflict	234	1	E → K in S67453. Ref.5
	Sequence conflict	258	1	S → T in CAA90277. Ref.1
	Sequence conflict	360	1	D → S in CAA90277. Ref.1
	Sequence conflict	461	1	F → N in CAA90277. Ref.1
	Sequence conflict	554	1	G → A in CAA90277. Ref.1
	Sequence conflict	575	1	T → S in CAA90277. Ref.1
	Sequence conflict	583	1	I → T in CAA90277. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48318-1 [UniParc]. Last modified December 13, 2002. Version 2. Checksum: 82D1AAF216F25100	FASTA	593	66,648
	10 20 30 40 50 60 MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL QRTNSLEEKS 70 80 90 100 110 120 RLVSAFRERQ SSKNLLSCEN SDQGARFRRT ETDFSNLFAQ DLLPAKNGEE QTAQFLLEVV 130 140 150 160 170 180 DILLNYVRKT FDRSTKVLDF HHPHQLLEGM EGFNLELSDH PESLEQILVD CRDTLKYGVR 190 200 210 220 230 240 TGHPRFFNQL STGLDIIGLA GEWLTSTANT NMFTYEIAPV FVLMEQITLK KMREIVGWSN 250 260 270 280 290 300 KDGDGIFSPG GAISNMYSIM AARYKYFPEV KTKGMAAVPK LVLFTSEHSH YSIKKAGAAL 310 320 330 340 350 360 GFGTDNVILI KCNERGKIIP ADLEAKILDA KQKGYVPLYV NATAGTTVYG AFDPIQEIAD 370 380 390 400 410 420 ICEKYNLWLH VDAAWGGGLL MSRKHRHKLS GIERANSVTW NPHKMMGVLL QCSAILVKEK 430 440 450 460 470 480 GILQGCNQMC AGYLFQPDKQ YDVSYDTGDK AIQCGRHVDI FKFWLMWKAK GTVGFENQIN 490 500 510 520 530 540 KCLELADYLY AKIKNREEFE MVFDGEPEHT NVCFWYIPQS LRGVPDSPER REKLHRVAPK 550 560 570 580 590 IKALMMESGT TMVGYQPQGD KANFFRMVIS NPAATQSDID FLIEEIERLG QDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification of the 62 kd form of glutamic acid decarboxylase from the mouse." Katarova Z., Szabo G., Mugnaini E., Greenspan R. Eur. J. Neurosci. 2:190-202(1990) [PubMed: 12106047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	Aust G., Steinbrenner H., Thamm B., Rost A.K., Seissler J. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[3]	"High-throughput sequence identification of gene coding variants within alcohol-related QTLs." Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M. Mamm. Genome 12:657-663(2001) [PubMed: 11471062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ILS and ISS.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
[5]	"Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat." Faulkner-Jones B.E., Cram D.S., Kun J., Harrison L.C. Endocrinology 133:2962-2972(1993) [PubMed: 8243324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 198-403. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49976 mRNA. Translation: CAA90277.1. Y12257 mRNA. Translation: CAA72934.1. AF483492 mRNA. Translation: AAL90766.1. AF483493 mRNA. Translation: AAL90767.1. BC027059 mRNA. Translation: AAH27059.1. S67453 mRNA. No translation available.
IPI	IPI00318496.
PIR	S61534.
RefSeq	NP_032103.2.
UniGene	Mm.272120
3D structure databases
SMR	P48318. Positions 92-592.
ModBase	Search...
Protein-protein interaction databases
STRING	P48318.
PTM databases
PhosphoSite	P48318.
Proteomic databases
PRIDE	P48318.
Genome annotation databases
Ensembl	ENSMUST00000094934; ENSMUSP00000092539; ENSMUSG00000070880; Mus musculus. [Genome view]
GeneID	14415.
KEGG	mmu:14415.
UCSC	uc008jzk.1. mouse.
Organism-specific databases
CTD	14415.
MGI	MGI:95632. Gad1.
Phylogenomic databases
HOGENOM	P48318.
HOVERGEN	P48318.
OMA	GWSNKDG.
Enzyme and pathway databases
BRENDA	4.1.1.15. 244.
Gene expression databases
ArrayExpress	P48318.
Bgee	P48318.
CleanEx	MM_GAD1.
Genevestigator	P48318.
GermOnline	ENSMUSG00000070880. Mus musculus.
Family and domain databases
InterPro	IPR002129. PyrdxlP-dep_de-COase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
PROSITE	PS00392. DDC_GAD_HDC_YDC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	285997.
SOURCE	Search...

Entry information

Entry name

DCE1_MOUSE

Accession

Primary (citable) accession number: P48318
Secondary accession number(s): O08685

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 13, 2002
Last modified:	November 3, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)