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Protein

Glutamate decarboxylase 1

Gene

Gad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei566SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carboxylic acid metabolic process Source: InterPro
  • locomotory exploration behavior Source: MGI
  • neurotransmitter biosynthetic process Source: UniProtKB-KW
  • social behavior Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.1.1.15. 3474.
ReactomeiR-MMU-888568. GABA synthesis.
R-MMU-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 1 (EC:4.1.1.15)
Alternative name(s):
67 kDa glutamic acid decarboxylase
Short name:
GAD-67
Glutamate decarboxylase 67 kDa isoform
Gene namesi
Name:Gad1
Synonyms:Gad67
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95632. Gad1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • axon terminus Source: MGI
  • cell cortex Source: MGI
  • cytoplasm Source: MGI
  • inhibitory synapse Source: MGI
  • intracellular Source: MGI
  • mitochondrion Source: MGI
  • neuron projection terminus Source: MGI
  • presynaptic active zone Source: MGI
  • synapse Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001469641 – 593Glutamate decarboxylase 1Add BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineCombined sources1
Modified residuei404N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48318.
PeptideAtlasiP48318.
PRIDEiP48318.

PTM databases

iPTMnetiP48318.
PhosphoSitePlusiP48318.
SwissPalmiP48318.

Expressioni

Gene expression databases

BgeeiENSMUSG00000070880.
CleanExiMM_GAD1.
ExpressionAtlasiP48318. baseline and differential.
GenevisibleiP48318. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP48318. 3 interactors.
MINTiMINT-4092862.
STRINGi10090.ENSMUSP00000092539.

Structurei

3D structure databases

ProteinModelPortaliP48318.
SMRiP48318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni189 – 191Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiKOG0629. Eukaryota.
COG0076. LUCA.
GeneTreeiENSGT00760000119205.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiP48318.
KOiK01580.
OMAiTNLRPPG.
OrthoDBiEOG091G07ZU.
PhylomeDBiP48318.
TreeFamiTF314688.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL
60 70 80 90 100
QRTNSLEEKS RLVSAFRERQ SSKNLLSCEN SDQGARFRRT ETDFSNLFAQ
110 120 130 140 150
DLLPAKNGEE QTAQFLLEVV DILLNYVRKT FDRSTKVLDF HHPHQLLEGM
160 170 180 190 200
EGFNLELSDH PESLEQILVD CRDTLKYGVR TGHPRFFNQL STGLDIIGLA
210 220 230 240 250
GEWLTSTANT NMFTYEIAPV FVLMEQITLK KMREIVGWSN KDGDGIFSPG
260 270 280 290 300
GAISNMYSIM AARYKYFPEV KTKGMAAVPK LVLFTSEHSH YSIKKAGAAL
310 320 330 340 350
GFGTDNVILI KCNERGKIIP ADLEAKILDA KQKGYVPLYV NATAGTTVYG
360 370 380 390 400
AFDPIQEIAD ICEKYNLWLH VDAAWGGGLL MSRKHRHKLS GIERANSVTW
410 420 430 440 450
NPHKMMGVLL QCSAILVKEK GILQGCNQMC AGYLFQPDKQ YDVSYDTGDK
460 470 480 490 500
AIQCGRHVDI FKFWLMWKAK GTVGFENQIN KCLELADYLY AKIKNREEFE
510 520 530 540 550
MVFDGEPEHT NVCFWYIPQS LRGVPDSPER REKLHRVAPK IKALMMESGT
560 570 580 590
TMVGYQPQGD KANFFRMVIS NPAATQSDID FLIEEIERLG QDL
Length:593
Mass (Da):66,648
Last modified:December 13, 2002 - v2
Checksum:i82D1AAF216F25100
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133R → H in CAA90277 (PubMed:12106047).Curated1
Sequence conflicti234E → K in S67453 (PubMed:8243324).Curated1
Sequence conflicti258S → T in CAA90277 (PubMed:12106047).Curated1
Sequence conflicti360D → S in CAA90277 (PubMed:12106047).Curated1
Sequence conflicti461F → N in CAA90277 (PubMed:12106047).Curated1
Sequence conflicti554G → A in CAA90277 (PubMed:12106047).Curated1
Sequence conflicti575T → S in CAA90277 (PubMed:12106047).Curated1
Sequence conflicti583I → T in CAA90277 (PubMed:12106047).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49976 mRNA. Translation: CAA90277.1.
Y12257 mRNA. Translation: CAA72934.1.
AF483492 mRNA. Translation: AAL90766.1.
AF483493 mRNA. Translation: AAL90767.1.
BC027059 mRNA. Translation: AAH27059.1.
S67453 mRNA. No translation available.
CCDSiCCDS16108.1.
PIRiS61534.
RefSeqiNP_032103.2. NM_008077.5.
XP_006498828.1. XM_006498765.3.
XP_006498829.1. XM_006498766.3.
UniGeneiMm.272120.

Genome annotation databases

EnsembliENSMUST00000094934; ENSMUSP00000092539; ENSMUSG00000070880.
GeneIDi14415.
KEGGimmu:14415.
UCSCiuc008jzk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49976 mRNA. Translation: CAA90277.1.
Y12257 mRNA. Translation: CAA72934.1.
AF483492 mRNA. Translation: AAL90766.1.
AF483493 mRNA. Translation: AAL90767.1.
BC027059 mRNA. Translation: AAH27059.1.
S67453 mRNA. No translation available.
CCDSiCCDS16108.1.
PIRiS61534.
RefSeqiNP_032103.2. NM_008077.5.
XP_006498828.1. XM_006498765.3.
XP_006498829.1. XM_006498766.3.
UniGeneiMm.272120.

3D structure databases

ProteinModelPortaliP48318.
SMRiP48318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48318. 3 interactors.
MINTiMINT-4092862.
STRINGi10090.ENSMUSP00000092539.

PTM databases

iPTMnetiP48318.
PhosphoSitePlusiP48318.
SwissPalmiP48318.

Proteomic databases

PaxDbiP48318.
PeptideAtlasiP48318.
PRIDEiP48318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094934; ENSMUSP00000092539; ENSMUSG00000070880.
GeneIDi14415.
KEGGimmu:14415.
UCSCiuc008jzk.1. mouse.

Organism-specific databases

CTDi2571.
MGIiMGI:95632. Gad1.

Phylogenomic databases

eggNOGiKOG0629. Eukaryota.
COG0076. LUCA.
GeneTreeiENSGT00760000119205.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiP48318.
KOiK01580.
OMAiTNLRPPG.
OrthoDBiEOG091G07ZU.
PhylomeDBiP48318.
TreeFamiTF314688.

Enzyme and pathway databases

BRENDAi4.1.1.15. 3474.
ReactomeiR-MMU-888568. GABA synthesis.
R-MMU-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

PROiP48318.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070880.
CleanExiMM_GAD1.
ExpressionAtlasiP48318. baseline and differential.
GenevisibleiP48318. MM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCE1_MOUSE
AccessioniPrimary (citable) accession number: P48318
Secondary accession number(s): O08685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 13, 2002
Last modified: November 2, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.